Structure of PDB 7wtv Chain T Binding Site BS01

Receptor Information

>7wtv Chain T (length=144) Species: 9606 (Homo sapiens)

PGVTVKDVNQQEFVRALAAFLKKSGKLKVPEWVDTVKLAKHKELAPYDEN
WFYTRAASTARHLYLRGGAGVGSMTKIYGGRQRNGVMPSHFSRGSKSVAR
RVLQALEGLKMVEKDQDGGRKLTPQGQRDLDRIAGQVAAANKKH

Ligand information

>7wtv Chain 2 (length=1574)

uaccugguugauccugccaguagcauaugcuugucucaaagauuaagcca
ugcaugucuaaguacgcacggccgguacagugaaacugcgaauggcucau
uaaaucaguuaugguuccuuuggucgcucguacuuggauaacugugguaa
uucuagagcuaauacaugccgacgggcgcugacccccuucgcggggggga
ugcgugcauuuaucaguggugacucuagauaaccucgggccgaucgcacg
ccggcggcgacgacccauucgaacgucugcccuaucaacuuucgauggua
gucgccgugccuaccauggugaccacgggugacggggaaucaggguucga
uuccggagagggagccugagaaacggcuaccacauccaaggaaggcagca
ggcgcgcaaauuacccacucccgacccggggagguagugacgaaaaauaa
caauacaggacucuuucgaggcccuguaauuggaaugaguccacuuuaaa
uccuuuaacgaggauccauuggagggcaagucuggugccaauuccagcuc
caauagcguauauuaaaguugcugcaguuaaaaagcucguaguuggaucu
cucucggccgaagcguuuacuuugaaaaaauuagaguguucaaagcaggc
cgccuggauaccgcagcuaggaauaauggaauaggaccgcgguucuauuu
uguugguuuucggaacugaggccaugauuaagagggacggccgggggcau
ucguauugcgccgcuagaggugaaauucuuggaccggcgcaagacggacc
agagcgaaagcauuugccaagaauguuuucauuaaucaagaacgaaaguc
ggagguucgaagacgauaccgucguaguuccgaccauaaacgaugccgac
cggcgaugcggcggcguuauucccaugacccgccgggcagcuuccgggaa
accaaagucuuuggguuccggggggaguaugguugcaaagcugaaacuua
aaggaauugacggaagggcaccaccaggaguggagccugcggcuuaauuu
gacccggcccggacacggacaggauugacagauugauagcucuuucucga
uuccgugggugguggugcauggccguuguuggcugguuaauuccgaugaa
cgagacucuggcaugcuauucuuagagggacaaguggcguucagccaccc
gagauugagcuaacaggucugugaugcccuguccggggcugcacgcgcgc
uacacugacuggcucagcguguccuacgccggcaggcgcggguaacccgu
ugaaccccauucgugauggggaucggggauugcaauuauuccccaugaac
gaggaauucccaguaagugcgggucauaagcuugcguugauuaagucccu
gcccuuuguacacaccgcccgucgcuacuaccgauuggaugguuuaguga
ggcccucggaucggccccgccggggugcccuggcggagcgcugagaagac
ggucgaacuugacuaucuagaggaaguaaaagucgagguuuccguaggug
aaccugcggaaggaucauuaacgg

...<<<<<.[[((((>>>>><<<.<<<<<<...<.<<..<......<<<.
<<<..<<....<<....<<..........>>...>>.>>......<<...
.....<<<.<....<<....<<<<<.........<<.....<<.<<<...
..>>>.>>......>>.........<<<<...<<<<<<....>>>>>>..
.>>>><<..<<<<<...<......>..>>>>>......>>...<<<<.<<
<..>>>>>>>.>>.>>>...>>..>.>>>.<<<....<<<....<<<<<<
<.........>>>>>>>>>>......>>>...<<<.<<<<....>>>>..
..>>>.>>.<<.<<<..........>>>.>>.<.<<<..>>>.>...>>>
>>>.........<<<....<<<<...>>>>..>>>..>...>>.>.....
<<<<<<<<<.<<<....>>>..>>>>>>.>>>......<<..<.......
....>..>>.........<<<<<........<<<<........>>>>.>>
>>>..>>>>>>.>>>.........<.<((.....<.<<...<<<.<<...
<<.<<<..>>>>>...<<<<<<.<.......<<...<.......>.<<<<
.>>>>...>>......>.>>>..>>>........<.<<.<<<<<<<....
...........>>>>>>>.>>.>....>>....<<<<<<..<...<<<<.
.<<<.<<<<<<<<...<<<......>>>......>>>>>>>>.>>>....
...<<....>>...>>>>..>..>>>.>>>...>>>...>>.>....<<<
<<<<...<...<<<<....>>>>...>>>>>>>>..........<<<.<<
.<<<..<.<<<<<<.<<<<......>>>>>>>>>>.>..>>>...<<..)
)>>...>>.....>>>.>.>.<<<......<<<<....>>>>....>>>.
.)))).]]....<<<<<<<<..<<..<<<<<..<<<.<<<<<<.......
..<<<<<....<.<<<<<<.......<<.<<<........>>>.>>....
.>>>>>>..>...<<<.<<.<<<<<<.<<<...<<<......>>>...>>
>....<<<...<...<<<....>>>..>.....<<<<<.....>>>>>..
......>>>...>>.>>>>..>>..>>>....>>>>>...>>.>>>>.>>
>.....<<.<<<........<.<<<.<<<<....>>>>.>>>....>...
......>>>.>>......<.....<<<<<..........>>>>>.....>
.....>>>>>.....<<<<<<<<<.....>>>>>>>>>......>>...>
>>>>>>>......<<<...<<....<<<<.<<....<<<<<<<<.<<<..
<<<<.<<...<<<<<.<<<<<<<<<..>>>>>>>>>...>>>>>...>>.
>>>>..>>>.>>>>>>>>...>>.>>>>.....>>.>>><<<<<<<<<..
..>>>>>>>>>.............

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7wtv The nucleoplasmic phase of pre-40S formation prior to nuclear export.
• Resolution: 3.5 Å
• Binding residue (original residue number in PDB): Q11 Q12 W33 V37 K41 K43 P47 Y48 R56 S59 R62 H63 R67 G71 V72 G73 S74 K77 I78 R82 R84 G86 V87 M88 H91 F92 S96 K97 S98 R101 Q105 R121
• Binding residue (residue number reindexed from 1): Q10 Q11 W32 V36 K40 K42 P46 Y47 R55 S58 R61 H62 R66 G70 V71 G72 S73 K76 I77 R81 R83 G85 V86 M87 H90 F91 S95 K96 S97 R100 Q104 R120

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0017134 fibroblast growth factor binding
• GO:0019901 protein kinase binding
• GO:0042802 identical protein binding

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0002181 cytoplasmic translation
• GO:0002548 monocyte chemotaxis
• GO:0006364 rRNA processing
• GO:0006412 translation
• GO:0007000 nucleolus organization
• GO:0030218 erythrocyte differentiation
• GO:0030490 maturation of SSU-rRNA
• GO:0031640 killing of cells of another organism
• GO:0042274 ribosomal small subunit biogenesis
• GO:0050829 defense response to Gram-negative bacterium
• GO:0060265 positive regulation of respiratory burst involved in inflammatory response
• GO:0060266 negative regulation of respiratory burst involved in inflammatory response
• GO:0061844 antimicrobial humoral immune response mediated by antimicrobial peptide

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0005925 focal adhesion
• GO:0014069 postsynaptic density
• GO:0016020 membrane
• GO:0022626 cytosolic ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:0032040 small-subunit processome
• GO:0070062 extracellular exosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7wtv, PDBe:7wtv, PDBj:7wtv
• PDBsum: 7wtv
• PubMed: 36321656
• UniProt: P39019|RS19_HUMAN Small ribosomal subunit protein eS19 (Gene Name=RPS19)