Structure of PDB 7s9u Chain T Binding Site BS01

Receptor Information

>7s9u Chain T (length=92) Species: 1423 (Bacillus subtilis)

MKDPRDVLKRPVITERSADLMTEKKYTFEVDVRANKTEVKDAVESIFGVK
VDKVNIMNYKGKSKRVGRYTGMTSRRRKAIVKLTADSKEIEI

Ligand information

>7s9u Chain A (length=2436)

uuaaguuagaaagggcgcacgguggaugccuuggcacuaggagccgauga
aggacgggacgaacaccgauaugcuucggggagcuguaagcaagcuuuga
uccggagauuuccgaauggggaaacccaccacucguaauggagugguauc
cauaucugaauucauaggauaugagaaggcagacccggggaacugaaaca
ucuaaguacccggaggaagagaaagcaaaugcgauucccugaguagcggc
gagcgaaacgggaucagcccaaaccaagaggcuugccucuugggguugua
ggacacucuguacggaguuacaaaggaacgagguagaugaagaggucugg
aaaggcccgccauaggagguaacagcccuguagucaaaacuucguucucu
ccugaguggauccugaguacggcggaacacgugaaauuccgucggaaucc
gggaggaccaucucccaaggcuaaauacucccuagugaccgauagugaac
caguaccgugagggaaaggugaaaagcaccccggaaggggagugaaagag
auccugaaaccgugugccuacaaguagucagagcccguuaacgggugaug
gcgugccuuuuguagaaugaaccggcgaguuacgaucucgugcaagguua
agcagaagaugcggagccgcagcgaaagcgagucugaauagggcgcauga
guacguggucguagacccgaaaccaggugaucuacccauguccaggguga
aguucagguaacacugaauggaggcccgaacccacgcacguugaaaagug
cggggaugagguguggguaggggugaaaugccaaucgaaccuggagauag
cugguucucuccgaaauagcuuuagggcuagccucaagguaagagucuug
gagguagagcacugauuggacuaggcgaauucagucaaacuccgaaugcc
aaugacuuauccuugggagucagacugcgagugauaagauccguagucga
aagggaaacagcccagaccgccagcuaaggucccaaaguauacguuaagu
ggaaaaggauguggaguugacucugcgccgaaaauguaccggggcuaaac
guaucaccgaagcugcggacuguucuucgaacagugguaggagagcguuc
uaagggcugugaagccagaccggaaggacugguggagcgcuuagaaguga
gaaugccgguaugaguagcgaaagaggggugagaauccccuccaccgaau
gccuaagguuuccugaggaaggcucguccgcucaggguuagucgggaccu
aagccgaggccgaaaggcguaggcgauggacaacagguugauauuccugu
accaccuccucaccauuugagcaauggggggacgcaggaggauaggguaa
gcgcgguauuggauauccgcguccaagcaguuaggcugggaaauaggcaa
auccguuucccauaagccugagcugugauggcgagcgaaauauaguagcg
aaguuccugauuccacacugccaagaaaagccucuagcgaggugagaggu
gcccguaccgcaaaccgacacagguaggcgaggagagaauccuaagguga
ucgagagaacucucguuaaggaacucggcaaaaugaccccguaacuucgg
gagaaggggugcucuguuagggugcaagcccgagagagccgcagugaaua
ggcccaggcgacuguuuagcaaaaacacaggucucugcgaagccguaagg
cgaaguauaggggcugacgccuaggcgcaacgaucugggcacugucucaa
cgagagacucggugaaauuauaguaccugugaagaugcagguuacccgcg
acaggacggaaagaccccguggagcuuuacugcagccugauauugaaugu
ccuucuaacccgccgcccuuaucgggcggggagacagugucaggugggca
guuugacuggggcggucgccuccuaaaagguaacggaggcgcccaaaggu
ucccucagaaugguuggaaaucauucgcagaguguaaaggcacaagggag
cuugacugcgagaccuacaagucgagcagggacgaaagucgggcuuagug
auccggugguuccgcauggaagggccaucgcucaacggauaaaagcuacc
ccgguccgucgcgggcgcaggaaauuugagaggagcuguccuuaguacga
gaggaccgggauggacgcaccgcugguguaccaguuguucugccaagggc
aucgcuggguagcuaugugcggacgggauaagugcugaaagcaucuaagc
augaagccccccucaagaugagauuucccauuccgcaaggaaguaagauc
ccugaaagaugaucagguugauaggucugagguggaaguguggcgacaca
uggagcugacagauacuaaucgaucgaggacuuaac

<<<<<<......<<<<<<<<<<<....<<<..<<<<<<<<<<<<<.....
.<<<<<<.....((.>>>.....<<<<<<<..<<<)).....>>>.....
>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<
<<<<<<<.........>>>>>>>.>>.>>....<<<<<<.....<.....
.>......>>>>>.>.........<<....>>...<<<<......<<...
..>>.....>>>>...<<<....<<<<<<<<....>>>>>>>>.......
<<<<<<<<.....<<<<.......<<<<<<<<<<.<<<.....<<<<...
...>>>>.((......<<<.....))>>>...>>>...>>>>>>>>>>>>
>>.>>>>>..>>>......<<<<<<<<.........>>>>>>>>......
<<<<<..((((>>>>>..>>>......>>.>>>>>>>.>>..........
.....<<....>>....>>.........<<<<....>>>>..........
...>....>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>
>>...<.<........>.>.<<<<<<....<<<<<<<.<<<<<..<<<..
.<<<.....>>>...>>>...<<....>>..<<<<.....>>>>......
>>>>>.>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<...
.<<<<<<......>>>>>>.....>>>...<<<.<<<<<........>>>
>>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>...>..
>.>>..<<<<<<....<<<<<....>>>>><<.<<<<<<...<<<<<<<<
<......<<...<<<.<<.............>>.>>>..>>.......>>
>>.>>>>>..>>>>>>..>>..<<<<<<<.<<......>>.>>>>>>>..
..<<<......>>>...<<<<.<<<...<<<(((.....<<<<<<.....
......<<..<<<<<<<..>>>>>>>.>>...........))).....>>
>>>>.>>>...>>>>>>><<<<<<<...>>>>>>>....>>>>>><.<<<
<<<<.<<......<<<<<.<<....>>.>>>>>...>>.>>>>>>>..>.
....>>>>>><......<<....<<<<<<.......>>>>>><<<<....
<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<.<<.
..<<<...<<<....>>>...>>>...>>...<<<<<........>>>>>
))<<<<<.<<<<<<......<<...<<<<....<<<<..<<<..<<<.<.
<<<.<.....[....>.>>>.<...<.....<<<<<.<<<<<<<.<<...
..>>>>>>>>>....>>>>>.....>....><..<<...........>>.
..>>.>>>...>>>...>>>>.........>>>>..>>..>>>>>>>>>>
>>>>>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>.
.<<<<....<<<<<<<..<<<........<.....<<<<<....<<..>>
.....>>>>><<<<.....<<<<....>>>>....>>>>...........
.<<<<<<<<<..<<<.........>>>..<<<<<<<.....<<<....>>
>.......>>>>>>>..<<<<..>>>>...>>.>>>>>>>..>.>>>..>
>>>>>>.>>>>>>.....>.....<<<<<........>>>>>..<<<<<<
<<.<<<..............<<<<<<..<<<<<.<<<<<<<<<.<<....
...>>....<<.<<<<<.......>>>>>>>.....>>>>>>>>>..>>>
>>..<<<.......>>><<<<<<...........>>>>>>..<<.....<
<<<<....<<<<<.......>>>>>......<..[.(((.>....>>>>>
.....<<<<..<<<......>>>..>>>>.<<<....>>>.>>)))]...
...<<<<<<<.<<..))))..>>>>>>>>>.............>>>>..>
>...>>>>>>>>>>>....<<<<<<<...<<<<..<<<<<<<.....<..
..>....>>>>>>>.<<<<<...<<<<...<<<<<<<<<<<.]..>>>>>
>..>>>>>...>>>>.>>>>>...<<<.<..<<<<<...........>>>
>>...>>>>.>>>>.......>>>>>>>..<<<<....>>>>....<<<<
<<<<<.......>>>>>....<<<<<<<<.<<<....<<<<<....>>>>
>...>>>..>>>>>.>>>...>>>>....>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7s9u RbgA ensures the correct timing in the maturation of the 50S subunits functional sites.
• Resolution: 3.2 Å
• Binding residue (original residue number in PDB): R16 R33 N35 K36 T37 N55 Y59 K60 R65 Y69 T70 T73 S74 R75 R77 K78 I80 K82
• Binding residue (residue number reindexed from 1): R16 R33 N35 K36 T37 N55 Y59 K60 R65 Y69 T70 T73 S74 R75 R77 K78 I80 K82

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7s9u, PDBe:7s9u, PDBj:7s9u
• PDBsum: 7s9u
• PubMed: 35141754
• UniProt: P42924|RL23_BACSU Large ribosomal subunit protein uL23 (Gene Name=rplW)