Structure of PDB 7qws Chain T Binding Site BS01

Receptor Information

>7qws Chain T (length=159) Species: 9986 (Oryctolagus cuniculus)

TNTKGKRRGTRYMFSRPFRKHGVVPLATYMRIYKKGDIVDIKGMGTVQKG
MPHKCYHGKTGRVYNVTQHAVGIVVNKQVKGKILAKRINVRIEHIKHSKS
RDSFLKRVKENDQKKKEAKEKGTWVQLKRQPAPPREAHFVRTNGKEPELL
EPIPYEFMA

Ligand information

>7qws Chain 5 (length=3493)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggagaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccccgcggcggggcgcgggacau
guggcguacggaagacccgcuccccggcgccgcucguggggggcccaagu
ccuucugaucgaggcccagcccguggacggugugaggccgguagcggccc
ccggcgcgccgggcccgggucuucccggagucggguugcuugggaaugca
gcccaaagcgggugguaaacuccaucuaaggcuaaauaccggcacgagac
cgauagucaacaaguaccguaagggaaaguugaaaagaacuuugaagaga
gaguucaagagggcgugaaaccguuaagagguaaacgggugggguccgcg
caguccgcccgguucaacccggcggcggguccggccgugucggcgggucc
cccgaccggcgaccggccgccgccgggcgcauuuccaccgcggcggugcg
ccgcgaccggcuccgggacggcugggaaggcccggcggggaagguggcuc
ggccgaguguuacagcccccccggcagcagcacucgccgaaucccggggc
cgagggagcgagacccgucgccgcgcucuccccccucgggggggccgggc
caccccucccacggcgcgaccgcucggggcggacuguccccagugcgccc
cgggcgggucgcgccgucgggcccgggggaggggccacgcggcgagcgca
cggggucggcggcgacgucggcuacccacccgacccgucuugaaacacgg
accaaggagucuaacacgugcgcgagucgggggcucgcacgaaagccgcc
guggcgcaaugaaggugaaggccggcgcgcucgccggccgaggugggauc
ccgaggccucuccaguccgccgagggcgcaccaccggcccgucucgcccg
ccgcgccggggagguggagcacgagcgcacguguuaggacccgaaagaug
gugaacuaugccugggcagggcgaagccagaggaaacucugguggagguc
cguagcgguccugacgugcaaaucggucguccgaccuggguauaggggcg
aaagacuaaucgaaccaucuaguagcugguucccuccgaaguuucccuca
ggauagcuggcgcucucgcacacgcaguuuuauccgguaaagcgaaugau
uagaggucuuggggccgaaacgaucucaaccuauucucaaacuuuaaaug
gguaagaagcccggcucgcuggcgugagccgggcguggaauggagugccu
agugggccacuuuugguaagcagaacuggcgcugcgggaugaaccgaacg
ccggguuaaggcgcccgaugccgacgcucaucagaccccagaaaaggugu
ugguugauauagagccgaaucaacuagcccugaaaauggauggcgcugga
gcgucgggcccauacccggccgucgccggcagucgagaguggacgggagc
ccgcccccggagccccgcggacgcuacgccgcgacgaguaggagggccgc
ugcggugagccuugaagccuagggcgcgggcccggguggagccgccgcag
gugcagaucuuggugguaguagcaaauauucaaacgagaacuuugaaggc
cgaaguggagaaggguuccaugugaacagcaguugaacaugggucagucg
guccugagagaugggcgagcgccguuccgaagggacgggcgauggccucc
guugcccucggccgaucgaaagggagucggguucagauccccgaauccgg
aguggcggagaugggcgccgcgaggcguccagugcgguaacgcgaccgau
cccggagaagccggcgggagccccggggagaguucucuuuucuuugugaa
gggcagggcgcccuggaauggguucgccccgagagaggggcccgugccuu
ggaaagcgucgcgguuccggcggcguccggugagcucucgcuggcccuug
aaaauccgggggagaggguguaaaucucgcgccgggccguacccauaucc
gcagcaggucuccaaggugaacagccucuggcauguuggaacaauguagg
uaagggaagucggcaagccggauccguaacuucgggauaaggauuggcuc
uaagggcugggucggucgucggccggcgccuagcagccgacuuagaacug
gugcggaccaggggaauccgacuguuuaauuaaaacaaagcaucgcgaag
gcccgcggcggguguugacgcgaugugauuucugcccagugcucugaaug
ucaaagugaagaaauucaaugaagcgcggguaaacggcgggaguaacuau
gacucucuuaagguagccaaaugccucgucaucuaauuagugacgcgcau
gaauggaugaacgagauucccacugucccuaccuacuauccagcgaaacc
acagccaagggaacgggcuuggcggaaucagcggggaaagaagacccugu
ugagcuugacucuagucuggcacggugaagagacauguuuuuucacugac
ccggugaggcgggggggcgcucgcuucuggcgccaagcgcccggccgcgc
gccggccgggcgcgacccgcuccggggacagugccagguggggaguuuga
cuggggcgguacaccugucaaacgguaacgcagguguccuaaggcgagcu
cagggaggacagaaaccucccguggagcagaagggcaaaagcucgcuuga
ucuugauuuucaguacgaauacagaccgugaaagcggggccucacgaucc
uucugaccuuuuggguuuuaagcaggaggugucagaaaaguuaccacagg
gauaacuggcuuguggcggccaagcguucauagcgacgucgcuuuuugau
ccuucgaugucggcucuuccuaucauugugaagcagaauucaccaagcgu
uggauuguucacccacuaauagggaacgugagcuggguuuagaccgucgu
gagacagguuaguuuuacccuacugaugauguguuguugccaugguaauc
cugcucaguacgagaggaaccgcagguucagacauuugguguaugugcuu
ggcugaggagccaauggggcgaagcuaccaucugugggauuaugacugaa
cgccucuaagucagaaucccgcccaggcggaacgauacggcagcgccgcg
gagccucgguuggccucggauagccggucccccgcgggguccggugcgga
gugcccuucguccugggaaacggggcgcggccggagaggcggccgccccc
ucgcccgucacgcacgccguucguggggaaccuggcgcuaaaccauucgu
agacgaccugcuucugggucgggguuucguacguagcagagcagcucccu
cgcugcgaucuauugaaagucagcccucgacacaaggguuugu

.........................<<<<<<.....<......>......
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>>>>>>..<<<...<<<<...<<<<<<<<<<.....>>>>>>.>>>>...
..>>>>...<<<<<<<<<....<<<<<<<<.<......<<<<<<<<....
<<<<......>>>>.....((......<<<.....))>>>...>.>>>>>
>>..>>>>>>>>>...>>>>>>>>>.......<<<<<<<........>>>
>>>>.....<<<<<..((((((>>>>>..>>>..................
................<<....>>...............<<<......>>
>..................................<<<<<<<<..<<<..
....<<<<<<<......<<<<<<<<<<<...<<<....<<<<<.<....>
>>.>>>......>>>.>>>>>>>>>>>...........>>.>>>>>..<<
<<<<<..<<<<<<<<<<.<<<..........<<<<.<<<<......<<<<
<..>>>>>.......>>>>.>>>>............>>>...>>>>>>>>
>>....................<<<<<..<<<<<<<.>>>>>>>..<.<<
<.<<<<..<<<<<<<<<<<<<<<<<<<<<<<.<<<<....>>>>.>>>>>
>>>>>.>>>>>>>>>>>.>>....>.>>>...>>>..>......>>>>>.
......>>.>>>>>....>>>...>>>>>>>>..<<<<........>>>>
<<<<<<....<<<<<<<<<<<<<..<<<...<<<<........>>>>...
..>>>...<<....>>...<<<<<<<<....>>>>>>>>..<<<<<...<
<<..<<<...(((..[[.>>>..>>>....>>>>>.<<<<<<<<<.<<<<
......>>>>>>>>>>>.>>....>>>>>>>>>>>>>..<<.<..<<<<<
<<.....<<<<<<<<<..<<<<...<<<<<<<....>>>>>>>...>.>>
>...<<<..<<.<<........>>>>.>>>....>>>>>>>>>...<<..
.....>>......>>>>>>...>..>.>>..<<<<<<....<.<<<....
>>>.><<<<<<<<<<............<<<<<<<<<...<<<....<<<.
.<.<<<...<<<.<.........>.>>>.>>>.>..>>>..>.>>...>>
>>>>>...<<<<<<<<<..<...>.>>>>>>>>>...>>...>>>>>>>.
>>>..<<<<.<<<<<.....>>>>>.>>>>....<<<......>>>...<
<<<<<<...<<<(((.......<<<<<.....<<..........<<<.<<
<<<<<<<............>>>>>>>>>>>>..........))).>>...
>>>>>..>>>...>>>>>>><<<<<<.<<<...........]].)))...
...........................>>>>>>>>>....>>>>>>.<.<
<<<<<<<.<<......<<<<.<<<<....>>>>>>>>...>>>>>>>>>>
..>.....>>>>>><<.....<<.....<<<<<<.......>>>>>>.<<
<.....<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<
<<<<...<<....<<<....>>>..........<<.<<<<<<<<<...>>
>>>>>>>>>......>>....>>>..<<<<<........>>>>>))<<<<
....<<<<...<<<<<<<<....>>>>>>>>..<<<....>>>...<..<
<<<<<<...<<<<<<<<<<<<<<<<<<<<<<<..[[[..>>>>>>....<
<<<<.<<<<.<<<<.....<<<.....>>>.....>>>>>>>>..>>>>>
.....<<<<<<<.......>>>>>>>>>>>.>.>>>>>>>>>>>>.....
....>>>>>>>..>............>>>>.>>>>.>>>..>>.....<<
......>>>>>>>.<<<<.....>>>>..>>>....<<<<.....<<<<<
<<.<<.........<.....<<<<<...............>>>>><<.<<
<<<<<<<<<...<<<<<<.>>>>>>........>>>>>..>>>>>>.>>.
...........<<<<<<<<<..<<<.........>>>..<<<<<<<....
<<<<.....>>>>......>>>>>>>...<<..<<<..<<<<<<....<<
<.....<<<<....>>>>.....>>>.>>>>..>>>>>.<<<<.......
..>>>>.........<...........<<<<<.......>>>>>.....>
.....>>....>>.>>>>>>>..>..>>.>>>>>>>..>>>>>>....>>
....<<<<<........>>>>>....<<<<<..<<<.<<......<<<<.
<<<<<<..<<<<<.<<<<<<<<<.<<<<<<<<<<..>>>>>>>>>>...<
<<<<.....<<<<..<<...>>..............<<<<<<<<<<<...
..>>>>>>>>>>>..>>>>..>>>>>....>>>>>>>>>..>>>>>...<
<<.....>>>..<<<<<<...........>>>>>>..<<...<<<<<<<.
..<<<<<<.......>>>>>>.....<<...(((>>....>>>>>>>...
<<<..<<<<.......>>>>..>>>.<<<....>>>.>>)))......<<
<<<<<..<<<..)))))).>>>>>>>>>>.........>>>>..>>>>>>
.......<<.<.<.<<<<<<...<<.......>>...>>>>>>...>>.>
.>........<<<<.<<<<<<..<.<.<<<<<......>>>>>...>.>.
<<<.........>>>.....>>>>>..>...>>>.>.....<<<<<<<..
..>>>.>>>>....>>..>>>.>>>>>.....<<<<<<.<..<<<..<.<
<<<<.................>>>>>.><<<<....<<<<....<<.<<<
<<<<]]].>>>>>..>>.>>...>>>>...>>>><<<<<...<<<<<...
........>>>>>...>>>>>.>>>...>.>>>>>>......<<<<<<.<
<..<<<.....<<.........<<<<<..<<<<..>>>>.>>>>>.....
...>>....<<..<<<<....<<<<.<<<<<<<......>>>>>>>>>>>
...>>>>..>>..............>>>..>>>>>>>>.<<<<<.<....
.........>....<<.<<<<<<<.<<.....<<<<.<..<<<<<.....
.>>>>>..>>>>>....>>....>>>>.>>>.>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7qws Mechanism of signal sequence handover from NAC to SRP on ribosomes during ER-protein targeting.
• Resolution: 3.4 Å
• Binding residue (original residue number in PDB): T2 N3 T4 K5 G6 K7 R8 R9 G10 R12 Y13 M14 F15 S16 F19 H22 G23 D41 G46 T47 Q49 G51 H54 K55 Y57 G59 K60 T61 T68 Q69 H70 K78 V80 L85 K87 R88 I89 N90 R92 K100 S101 F105 V109 K129 R130 R142
• Binding residue (residue number reindexed from 1): T1 N2 T3 K4 G5 K6 R7 R8 G9 R11 Y12 M13 F14 S15 F18 H21 G22 D40 G45 T46 Q48 G50 H53 K54 Y56 G58 K59 T60 T67 Q68 H69 K77 V79 L84 K86 R87 I88 N89 R91 K99 S100 F104 V108 K128 R129 R141

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005783 endoplasmic reticulum
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7qws, PDBe:7qws, PDBj:7qws
• PDBsum: 7qws
• PubMed: 35201867
• UniProt: G1SHQ2|RL21_RABIT Large ribosomal subunit protein eL21 (Gene Name=RPL21)