Structure of PDB 6vwn Chain T Binding Site BS01

Receptor Information

>6vwn Chain T (length=93) Species: 562 (Escherichia coli)

FTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDHDKVMNMQ
AKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA

Ligand information

>6vwn Chain 3 (length=120)

ugccuggcggccguagcgcgguggucccaccugaccccaugccgaacuca
gaagugaaacgccguagcgccgaugguaguguggggucuccccaugcgag
aguagggaacugccaggcaa

<<<<<<<<<......<<<<<<<<....<<.<<<...............>>
>...>>...>>>>>>.>>.<<.......<<<<<<<<...>>>>>>>>...
....>>....>>>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6vwn mRNA stem-loops can pause the ribosome by hindering A-site tRNA binding.
• Resolution: 3.4 Å
• Binding residue (original residue number in PDB): R9 Q12 K14 S17 R18 R19 R21 Y31 Q78 H88
• Binding residue (residue number reindexed from 1): R8 Q11 K13 S16 R17 R18 R20 Y30 Q77 H87

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0008097 5S rRNA binding
• GO:0019843 rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0009314 response to radiation
• GO:0017148 negative regulation of translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6vwn, PDBe:6vwn, PDBj:6vwn
• PDBsum: 6vwn
• PubMed: 32427100
• UniProt: P68919|RL25_ECOLI Large ribosomal subunit protein bL25 (Gene Name=rplY)