Structure of PDB 6hrm Chain T Binding Site BS01

Receptor Information

>6hrm Chain T (length=94) Species: 562 (Escherichia coli)

MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIKAAVQKL
FEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLD

Ligand information

>6hrm Chain 1 (length=4450)

aaauugaagaguuugaucauggcucagauugaacgcuggcggcaggccua
acacaugcaagucgaacgguaacaggaagaagcuugcuucuuugcugacg
aguggcggacgggugaguaaugucugggaaacugccugauggagggggau
aacuacuggaaacgguagcuaauaccgcauaacgucgcaagaccaaagag
ggggaccuucgggccucuugccaucggaugugcccagaugggauuagcua
guaggugggguaacggcucaccuaggcgacgaucccuagcuggucugaga
ggaugaccagccacacuggaacugagacacgguccagacuccuacgggag
gcagcaguggggaauauugcacaaugggcgcaagccugaugcagccaugc
cgcguguaugaagaaggccuucggguuguaaaguacuuucagcggggagg
aagggaguaaaguuaauaccuuugcucauugacguuacccgcagaagaag
caccggcuaacuccgugccagcagccgcgguaauacggagggugcaagcg
uuaaucggaauuacugggcguaaagcgcacgcaggcgguuuguuaaguca
gaugugaaauccccgggcucaaccugggaacugcaucugauacuggcaag
cuugagucucguagagggggguagaauuccagguguagcggugaaaugcg
uagagaucuggaggaauaccgguggcgaaggcggcccccuggacgaagac
ugacgcucaggugcgaaagcguggggagcaaacaggauuagauacccugg
uaguccacgccguaaacgaugucgacuuggagguugugcccuugaggcgu
ggcuuccggagcuaacgcguuaagucgaccgccuggggaguacggccgca
agguuaaaacucaaaugaauugacgggggcccgcacaagcgguggagcau
gugguuuaauucgaugcaacgcgaagaaccuuaccuggucuugacaucca
cggaaguuuucagagaugagaaugugccuucgggaaccgugagacaggug
cugcauggcugucgucagcucguguugugaaauguuggguuaagucccgc
aacgagcgcaacccuuauccuuuguugccagcgguccggccgggaacuca
aaggagacugccagugauaaacuggaggaagguggggaugacgucaaguc
aucauggcccuuacgaccagggcuacacacgugcuacaauggcgcauaca
aagagaagcgaccucgcgagagcaagcggaccucauaaagugcgucguag
uccggauuggagucugcaacucgacuccaugaagucggaaucgcuaguaa
ucguggaucagaaugccacggugaauacguucccgggccuuguacacacc
gcccgucacaccaugggaguggguugcaaaagaaguagguagcuuaaccg
gucaacagccguuugagcuaaccgguacuaaugaaccgugaggcuuaacc
uugagagguuaagcgacuaagcguacacgguggaugcccuggcagucaga
ggcgaugaaggacgugcuaaucugcgauaagcgucgguaaggugauauga
accguuauaaccggcgauuuccgaauggggaaacccaguguguuucgaca
cacuaucauuaacugaauccauagguuaaugaggcgaaccgggggaacug
aaacaucuaaguaccccgaggaaaagaaaucaaccgagauucccccagua
gcggcgagcgaacggggagcagcccagagccugaaucaguguguguguua
guggaagcgucuggaaaggcgcgcgauacagggugacagccccguacaca
aaaaugcacaugcugugagcucgaugaguagggcgggacacgugguaucc
ugucugaauauggggggaccauccuccaaggcuaaauacuccugacugac
cgauagugaaccaguaccgugagggaaaggcgaaaagaaccccggcgagg
ggagugaaaaagaaccugaaaccguguacguacaagcagugggagcacgc
uuaggcgugugacugcguaccuuuuguauaaugggucagcgacuuauauu
cuguagcaagguuaaccgaauaggggagccgaagggaaaccgagucuuaa
cugggcguuaaguugcaggguauagacccgaaacccggugaucuagccau
gggcagguugaagguuggguaacacuaacuggaggaccgaaccgacuaau
guugaaaaauuagcggaugacuuguggcugggggugaaaggccaaucaaa
ccgggagauagcugguucuccccgaaagcuauuuagguagcgccucguga
auucaucuccggggguagagcacuguuucggcaagggggucaucccgacu
uaccaacccgaugcaaacugcgaauaccggagaauguuaucacgggagac
acacggcgggugcuaacguccgucgugaagagggaaacaacccagaccgc
cagcuaaggucccaaagucaugguuaagugggaaacgaugugggaaggcc
cagacagccaggauguuggcuuagaagcagccaucauuuaaagaaagcgu
aauagcucacuggucgagucggccugcgcggaagauguaacggggcuaaa
ccaugcaccgaagcugcggcagcgacgcuuaugcguuguuggguagggga
gcguucuguaagccugcgaaggugugcugugaggcaugcuggagguauca
gaagugcgaaugcugacauaaguaacgauaaagcgggugaaaagcccgcu
cgccggaagaccaaggguuccuguccaacguuaaucggggcagggugagu
cgaccccuaaggcgaggccgaaaggcguagucgaugggaaacagguuaau
auuccuguacuugguguuacugcgaaggggggacggagaaggcuauguug
gccgggcgacgguugucccgguuuaagcguguaggcugguuuuccaggca
aauccggaaaaucaaggcugaggcgugaugacgaggcacuacggugcuga
agcaacaaaugcccugcuuccaggaaaagccucuaagcaucagguaacau
caaaucguaccccaaaccgacacagguggucagguagagaauaccaaggc
gcuugagagaacucgggugaaggaacuaggcaaaauggugccguaacuuc
gggagaaggcacgcugauauguaggugaggucccucgcggauggagcuga
aaucagucgaagauaccagcuggcugcaacuguuuauuaaaaacacagca
cugugcaaacacgaaaguggacguauacggugugacgccugcccggugcc
ggaagguuaauugaugggguuagcgcaagcgaagcucuugaucgaagccc
cgguaaacggcggccguaacuauaacgguccuaagguagcgaaauuccuu
gucggguaaguuccgaccugcacgaauggcguaaugauggccaggcuguc
uccacccgagacucagugaaauugaacucgcugugaagaugcaguguacc
cgcggcaagacggaaagaccccgugaaccuuuacuauagcuugacacuga
acauugagccuugauguguaggauaggugggaggcuuugaaguguggacg
ccagucugcauggagccgaccuugaaauaccacccuuuaauguuugaugu
ucuaacguugacccguaauccggguugcggacagugucugguggguaguu
ugacuggggcggucuccuccuaaagaguaacggaggagcacgaagguugg
cuaauccuggucggacaucaggagguuagugcaauggcauaagccagcuu
gacugcgagcgugacggcgcgagcaggugcgaaagcaggucauagugauc
cggugguucugaauggaagggccaucgcucaacggauaaaagguacuccg
gggauaacaggcugauaccgcccaagaguucauaucgacggcgguguuug
gcaccucgaugucggcucaucacauccuggggcugaaguaggucccaagg
guauggcuguucgccauuuaaagugguacgcgagcuggguuuagaacguc
gugagacaguucggucccuaucugccgugggcgcuggagaacugaggggg
gcugcuccuaguacgagaggaccggaguggacgcaucacugguguucggg
uugucaugccaauggcacugcccgguagcuaaaugcggaagagauaagug
cugaaagcaucuaagcacgaaacuugccccgagaugaguucucccugacc
cuuuaaggguccugaaggaacguugaagacgacgacguugauaggccggg
uguguaaacggacaugguuggagggcgcuuaccacuuugugauucaugac
uggggugaagucguaacaagguaaccguaggggaaccugcgguuggauca

........<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<....
.<<<.<<<..<<<..<<.<......<<<<<<......>>>>>>.....>.
.>>>>>......<<.......<<<<<<<..<<...<<<<<<<.<.<<...
..<<<<<......>>>>>......>>.>.....<<<....>>>....<<<
<<<..<<....>>>>>>>>.>>>>>>>..>>.>>>>>>><<<....<<<.
.<<<<<<<.........>>>>>>>>>>......>>>..<<<<<<<<....
>>>>...>>>>.>>.<<<<<.<.........>>>>>>.<<<<....>>>>
...>>>>>>.........<<<...<<<<<....>>>>.>>>>..>>.>>>
>>>..<<<<......<<<<....>>>>.....>>>>...<.<<<<<....
..<<<<<<<<.<........>>>>>>>.>>.......>>>>>....>..<
<<<<(((...<<<<<.....<<.)))>>.......>>>>>>>>>>..>>>
>>>>>>..........<<<((.....<<<<...<<<.<<<<<<<.<<<<<
<<<<<......<<<<<<.....>>>>>>....>>>>>>>>..>>>>>>>>
>...<<<<<<<<...<<<<<<<....<<<<<<<<...<<<......>>>.
.....>>>>>>>>...........<<....>>.>>>>>>>..>>>.>>>>
>...>>>...>>>>....<<<<<<...<<...<<<<.<.....>.>>>>.
..>>>>>>>>..........<<<<<<.<<<<<<<<<<<<<.....>>>>>
>>>>>>>>..<<..))>>.....>>>>>>.>>>.<<<......<<<<...
.>>>>....>>>..)))).]<<<<<.<<<<<<<.<<.<<<<<<..<<<<<
<<<<<......<<........>>..........<<<<<<<......<<..
<<<..<<<<<<<....>>>>>>>...<......>..>>>...>>.<<<.<
<<..<<<<<<.......<<<<<<<<<....>>>..<<<<......>>>>.
.>>>>>>.....<<<<.<<<<<<<...<<..<<.......>>>>....>>
>>>>>.....<<<<<.....>>>>>........>>>>.........>>>.
..>>>>>>>>>...>>>>>>>...>>.>>>>>>>>.....<<<<<<<...
..<<<..<<...<<<....>>>...>>....>>>.....>>>>>>>....
..<....<<<<<<<........>>>>>>>....>.....>>>>>>....<
<<<<<<.........>>>>>>>......>>...>>>>>>>>>>.>>....
<..<<.<.<<<<.<<<..<<<<<<<<<<<<....<<<<<<.<<<<..<<.
........<<<.....<<...<<<<<.<<<.....<<..<..<<<<<<<<
<....>>>>>>>>>......<<<<<<<<<<<.....[[..[[<<<<<<<[
[<<......<<<<<<....(((.>>>.....<<<<<<<..<<<)))....
>>>......>>>>>>>...>>>....<<<....>>><<<<<<<....>>>
>>>>.<<<<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<
<....>>......>>>>>.>.........<<....>>..<<<<<<.....
<<.....>>....>>>>>>..(((..<<<<......<<<<<<<<<<<<<.
<<<...<<<<<......>>>>>((......<<<.....))>>>...>>>.
..>>>>>>>>>>>>>...>>>>........<<<<<<<<.........>>>
>>>>>......<<<<<..<<<<>>>>>..)))......]].>>>>>>>.]
]...............<<....>>....]].........<<<<.....>>
>>..................>>>>>>>>>>>.......<<<<..<<<<<<
....>>>>>>..>>>>...<.<........>.>.<<<<<<....<<<<<<
<<<<<<<..<<<...<<.....>>...>>>...<<....>>..<<<<...
..>>>>.....>>>>>>>>>>>>>..<<.<...<<<<<<.....<<<<<<
<<<..<<<....<<<<<<......>>>>>>.....>>>...<<<.<<<<<
........>>>>>>>>....>>>>>>>>>...<<<.....>>>......>
>>>>>......>.>>..<<<<<<....<<<<<....>>>>>.<<<<<<<<
...<<<<<<<<<.....<<<...<<<.<<<<...<<.<<<.......>>>
..>>...>>>>.>>>..>>.>.....>>>>>>.>>>...>>>>>>>.>..
.<<<<<<<<<<......>>>>>>>>>>....<<<......>>>...<<<<
.<<<...<<<(((.....<<<<<<...........<<..<<<.....<..
......<<<<<.<<...<<...........>>...>>........<<<..
....>>>..>>>>>....>.....>>>.>>...........))).....>
>>>>>.>>>...>>>>>>><<<<<<<<......>>>>>>>>....>>>>>
><.<<<<<...<<<......<<<<<<<<....>>>>>>>>...>>>..>>
>>>..>.....>>>>>><......<<.....<<<<<<.......>>>>>>
<<<<....<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((
<<<.<<<...<<<...<<<....>>>...>>>...>>>..<<<<<.....
...>>>>>))<<<<<<<<<<<<<...<<<<....<<<<..<<<..<<<<<
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.>>>>>>...>>>...>>>>.........>>>>...>>....>>>>>>>>
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>>>>..>>>>><<<<<<.......>>>>>>........<...........
<<<<<.......>>>>>.....>....>>>>>..>>.>>>>>>>.....>
>.>>>>>>>>.>>>>>>.....>.....<<<<<........>>>>>..<<
<<<<<<.(((.[[......<<<<.[[<<<<..<<<<<.<<<.<<<<<.<<
<<<<...<<..<<<...........<<<<<..<<<......<<..<<<<.
...>>>>..>>...>>>............>>>>>..>>>..>.>..>>>>
>>...<<<.<<<<<<.....>>>>>>>>>....>>>>>.>>>..>>>>>.
.<<<<.....>>>><<<<<<...........>>>>>>..<<...<<<<<<
<...<<<<<<.......>>>>>>.....<<...(((>>....>>>>>>>.
..<<<<..<<<<....>>>>..>>>>.<<<....>>>.>>))).......
<<<<<<<.((..>>>>..))>>>>>>>.............>>>>..]]>>
>>.......<<<<.<.<<<<<<...<<.......>>...>>>>>>...>>
>>.>.......<<<<<.<<<<<<..<<<<<<<<<......>>>>>>..>>
>.<<<<<.....>>>>>.....>>>>>..>...>>>.>>....<<<<<<<
....>>>.>>>>....]]..)))>>>>>>>>....<<<<<<<...<<<<.
.<<<<<<<...............>>>>>>>.<<<<<...<<<<...<<<<
<<<<<<<))).>>>>>>..>>>>>...>>>>.>>>>>...<<<.<..<<<
<<...........>>>>>...>>>>.>>>>.......>>>>>>>..<<<<
<.....>>>>>>....>>..<<<<<.......>>>>>....>>>>>>>>.
.>>.....>>>........>>.>>>>>>>>>>...>>>>>>>>>>>>..>
>>.>>>>..>.>>...>.....<<<<<<<<<....>>>>>>>>>......

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6hrm Controlling orthogonal ribosome subunit interactions enables evolution of new function.
• Resolution: 2.96 Å
• Binding residue (original residue number in PDB): M1 H15 S17 E18 K19 T39 K40 N59 L61 K64 K66 R73 G75 R76 R77 K82 Y84
• Binding residue (residue number reindexed from 1): M1 H15 S17 E18 K19 T39 K40 N59 L61 K64 K66 R73 G75 R76 R77 K82 Y84

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6hrm, PDBe:6hrm, PDBj:6hrm
• PDBsum: 6hrm
• PubMed: 30518861
• UniProt: P0ADZ0|RL23_ECOLI Large ribosomal subunit protein uL23 (Gene Name=rplW)