Structure of PDB 7d5t Chain Sc Binding Site BS01

Receptor Information

>7d5t Chain Sc (length=80) Species: 559292 (Saccharomyces cerevisiae S288C)

LVQDLLHPTAASEARKHKLKTLVQGPRSYFLDVKCPGCLNITTVFSHAQT
AVTCESCSTILCTPTGGKAKLSEGTSFRRK

Ligand information

>7d5t Chain SA (length=1189)

aagauaguuaucugguugauccugccaguagucauaugcuugucucaaag
auuaagccaugcaugucuaaguauaagcaauuuauacagugaaacugcga
aaaaucaguuaucguuuauuugauaguuccuuuacuacaugguauaacug
ugguaauucuagagcuaauacaugcuuaaaaucucgacccuuuggaagag
auguauuuauuagagucuucggacucuuugaugauucauaauaacuuuuc
gaaucgcauggccuugugcuggcgaugguucauucaaauuucugcccuau
caacuuucgaugguaggauaguggccuaccaugguuucaacgggagcagg
cgcgcaaauuacccaauccuaauucagggagguagugacaauaaauaacg
auacagggcccauucgggucuuguaauuggaaugaguacaauguaaauac
cuuaacgaggaacaauuggagggcaagucuggugccagcagccgcgguaa
uuccagcuccaauagcguauauuaagcucguaguugaacugggcccgguu
ggccgguccgauuuucgugacuggccaacggggccuuuccuucugguuua
cuuugaaaaaauuagaguguucaaagcaggcguauugcucgaauauauua
gcauggaauaauagaauaggguuugguucuaucuaggaccaugauuaaua
gggacggucgggggcaucaguauucaauugucagaggugaaauucuugga
uuuauugaagacuaacuacugcgaaagcauuugccaaggacguuuucauu
aaucaagaacguagggaucgggugguguuuuuuuaaugacccacucggca
ccuuggaguauggucgcaaggcugaaacuuaaaggaauugacggaagggc
accaccaggaguggagccugcggcgcgcgcuacacugacggagccagcga
aacuccgucgugcugggaacgaggaauuccuaguaagcgcaagucaucag
cuugcguugauuacgucccugcccuuuguacacaccgcccgucgcuagua
ccgauugaauggcuuagugaggccucaggaucugcuuagagaagggggca
acuccaucucagagcggagaauuuggacaaacuuggucauuuagaggaac
uaaauuccguaggugaaccugcggaaggaucauuaaaga

.................................<<<<<<...<.<.....
.....<<<.<<<..<<....<<.....................>>.>>..
.....<<<..<<..<<....<<<..................<.....<<.
<<<.....>>>.>>......>.........<<<<<..<<....>>..>>>
>><...<<<<<<..<<<<..>>>>..............>>>>>>.....>
...<<<<..<<<.....>>>.>>>>....>>>...>>>>..>>>.<<<..
..<<<....<<<<<<<<.......>>>>>>>>>>>......>>>.>>>>>
>.........<<<....<<<.....>>>..>>>.......>.>.....<<
.<<<<..<<<<....>>>>..>>>>..>>......<<..<..........
.>..>>.........<<<<<<.......<<<....>>>............
......>>>>>>..>>>>>>.....<.<<...<<<.<<...<<<<<.<<<
<<<<<<<<<<<...>>>.>>>>>>>>>>>.>>>>>............<<<
<<.<<...........<<.......>>.<<<.....>>>...........
.>>.>>..>>>............<..<.......>..>......>>....
<<<<<<.<<...<<<<..<<..<<<<<<.<...<<<......>>>.....
.>.>>>>>>..>>.......<<....>>...>>>>..>.>>>>.>>>...
>>>...>>.>..<<<..<<<<<<<<.<<..........>>>>>>>>>>..
>>>..................<<.........>>.....<<<<<<<<<<<
<..<<.<<<<<<..<<<.<<<<.>>>>.>>>.....<<<<<<<.......
..>>>>>>>......<...>.....>>>>>>....<<<<<<<........
.>>>>>>>......>>...>>>>>>>............>>>>>..<<<<.
<<....<<<<<<<<.<<<....<<..........<<<.<<<..<<<....
..>>>..>>>.>>>..........>>....>>>.>>>>>>>>...>>.>>
>>..<<<<<<<<<....>>>>>>>>>.............

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7d5t Cryo-EM structure of 90S preribosome with inactive Utp24 (state F1)
• Resolution: 6.0 Å
• Binding residue (original residue number in PDB): R17 H19 K20 Q26 S30 F47 H49 Q51 T67 G68 G69 K70 K72
• Binding residue (residue number reindexed from 1): R15 H17 K18 Q24 S28 F45 H47 Q49 T65 G66 G67 K68 K70

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0008270 zinc ion binding
• GO:0046872 metal ion binding

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:0044391 ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7d5t, PDBe:7d5t, PDBj:7d5t
• PDBsum: 7d5t
• UniProt: P35997|RS27A_YEAST Small ribosomal subunit protein eS27A (Gene Name=RPS27A)