Structure of PDB 8fl9 Chain SV Binding Site BS01

Receptor Information

>8fl9 Chain SV (length=139) Species: 9606 (Homo sapiens)

MRIEKCYFCSGPIYPGHGMMFVRNDCKVFRFCKSKCHKNFKKKRNPRKVR
WTKAFRKAAGKELTVDNSFEFEKRRNEPIKYQRELWNKTIDAMKRVEEIK
QKRQAKFIMNRLKKNKELQKVQDIKEVKQNIHLIRAPLA

Ligand information

>8fl9 Chain L3 (length=3361)

gcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguca
gcggaggagaagaaacuaaccaggauucccucaguaacggcgagugaaca
gggaagagcccagcgccgaauccccgccccggggcgcgggacauguggcg
uacggaagacccgcuccccggcgccgcgggggcccaaguccuucugaucg
aggcccagcccguggacggugugaggccgguagcggcccccggcgcgccg
ggcccgggucuucccggagucggguugcuugggaaugcagcccaaagcgg
gugguaaacuccaucuaaggcuaaauaccggcacgagaccgauagucaac
aaguaccguaagggaaaguugaaaagaacuuugaagagagaguucaagag
ggcgugaaaccguuaagaggaacgggugggguccgcgcaguccgcccgga
ggauucaacccggcggcggccggccgugucggcggcccggcggaucuggg
accgucccccgaccggcgaccggccgccgccgggcgcauuuccaccgcgg
cggugcgccgcgaccggcuccgggacggcugggaaggcccggcggggaag
guggcucgggcccgaguguuacagcccccccggcagcagcacucgccgaa
ucccggggccgagggaggucgccgcgcucuccccccggggggccgggcca
ccccucccacggcgcgaccgcucggggcggacuguccccagugcgccccg
ggcgggucgcgccgucgggcccggggggccacgcgcgcgucccccgaaga
gggggacggcggagcgagcgcacggggucggcggcgacgucggcuaccca
cccgacccgucuuacacggaccaaggagucuaacacgugcgcgagucggg
ggcucgcacgaaagccgccguggcgcaaugaaggugaaggccgcggccga
ggugggaucccgaggccucuccaguccgccgagggcgcaccaccggcccg
ucucgcccgccgcgccggggagguggagcacgagcgcacguguuaggacc
cgaaagauggugaacuaugccugggcagggcgaagccagaggaaacucug
guggagguccguagcgguccugacgugcaaaucggucguccgaccugggu
auaggggcgaaagacuaaucgaaccaucuaguagcugguucccuccgaag
uuucccucaggauagcuggcgcucuccgcaguuuuauccgguaaagcuuu
aaauggguaagaagcccggcucgcuggcguggagccgggcguggaaugcg
agugccuagugggccacuuuugcagaacuggcgcugcgggaugaaccgaa
cgccggguuaaggcgcccgaugccgacgcucaucagaccccaaaaggugu
ugguugauauagacagcaggacgguggccauggaagucggaauccgcuaa
ggaguguguaacaacucaccugccgaaucaacuagcccugaaaauggaug
gcgcuggagcgucgggcccauacccggccgucgccggcagucgagagugg
acgggagcggcgcgcggacgcuacgccgcgacgaguaggagggccgcugc
ggugagccuugaagccuagggcgcgggcccggguggagccgccgcaggug
cagaucuuggugguaguagcaaauauucaaacgagaacuuugaaggccga
aguggagaaggguuccaugugaacagcaguugaacaugggucagucgguc
cugagagaugggcgagcgccguuccgaagggacgggcgagcccugaucga
aagggagucggguucagauccccgaauccggaguggcggagaugggcgcc
gcgaggcguccagugcgguaacgcgaccgaucccggagaagccggcggga
gccccggggagaguucucuuuucuuugugaagggcagggcgcccuggaau
ggguucgccccgagagaggggcccgugccuuggaaagcgucgcgguuccg
gcggcguccggugagcucucgcuggcccuugaaaauccgggggagagggu
guaaaucucgcgccgggccguacccauauccgcagcaggucuccaaggug
aacagccucuggcauguuggaacaauguagguaagggaagucggcaagcc
ggauccguaacuucgggauaaggauuggcucuaagggcugggucggucgc
ggccggcgccuagcagccgacuuagaacuggugcggaccaggggaauccg
acuguuuaauuaaaacaaagcaucgcgaaggcccgcggcggguguugacg
cgaugugauuucugcccagugcucugaaugucaaagugaagaaauucaau
gaagcgcggguaaacggcaagugaacgagauucccacugucccuaccuac
uauccagcgaaaccacagccaagggcgggcuuggcggaaucagcggggaa
agaagacccuguugagcuugacucuagucuggcacggugaaguuuuuuca
cugacccggugaggcggggggcuucuggcgccaagcgcccggccgcgcgc
cggccgggcgcgacccgcuccggggacagugccagguggggaguuugacu
guacaccugucaaacgguaacgcagguguccuaaggcgagcucagggagg
acagaaaccucccguggagcagaagggcaaaagcucgcuugaucuugauu
uucaguacgaauacagaccgugaaagcggggccucacgauccuucugacc
uuuuggguuuuaagcaggaggugucagaaaaguuaccacagggaucuugu
ggcggccaagcgagcgacgucgcuuuuuggaugucggcucuuccuaucau
ugugaagcagaauucaccaagcguuggauuguucacccacuaauagggaa
cgugagcuggguuuagaccgucgugagacagguuaguuuuacccuacuga
ugauguguuguugccaugguaauccugcucaguacgagaggaaccgcagg
uucagacauuugguguaugugcuuggcugaggagccaauggggcgaagcu
accaucugugggauuaugacugaacgccucuaagucagaaucccgcccag
gcggaacgauagcgccgcggagccucgguuggccucggauagccgguccc
cgggguccggugcggagugcccuucguccugggaaacggggcgcggccgg
agaggcggccgcccccucgcccgucacgcaccgcacguucguggggaacc
uggcgcuaaaccauucguagacgaccugcuucugggucgggguuucguac
guagcagagcagcucccucgcugcgaucuauugaaagucagcccucgaca
caaggguuugu

........................<<<<<<.....<<....>>......>
>>>>.>.........<<....>>..<<<<<<.....<<.....>>....>
>>>>>..<<<..<<<<<...<<<<<<<<<..>>>>>.>>>>.....>>>>
>..<<<<<<<<<<...<<<<<<<<.<<<<<<<<<<....<<<<......>
>>>.....((......<<<.....))>>>...>.>>>>>>>.>>>>>>>>
>>..>>>>>>>>>>.......<<<<<<<........>>>>>>>.....<<
<<<..((((((>>>>>..>>>.............................
.....<<....>>...............<<<......>>>..........
......................<<<<<<<<..<<<.<<...<<<<<<<<.
<<<.....<<<<<<<<<<<<<<<<<<.<<<<<.<<...<<<<<.<<...>
>>>>>>>>>>>>>>>>>...>>>>>>>>>>>>>>......>>>.>>>.>>
>>>...<<<<<<<<<<<<<<<<<<<<<<<...<<..<<<.<<.<<<<...
...<<<<<<..>>>>>>.......>>>>>>.>>>........>>>>>>..
>>>>>>>>>>>............<<<<<<.<<<<<..>>>>>.<<<<<<.
<<<<.<<<<<<<<<<..<<<<<<<<<<<<<...........>.>>>>>>>
>>>>>...>>>>>>>.>>>...>>>>>>>>.>>..<<<<<<<<<......
.>>>>>>>.>>....>>>>>>.....>>>.>>>>>...>>>>>...>>>>
>>>>..<<<<.....>>>><<<<<<....<<<<<<<<<<<<<..<<<...
<<<<........>>>>.....>>>...<<....>>...<<<<..>>>>..
<<<<<...<<<..<<<..((((.((((>>>..>>>....>>>>>.<<<<<
<<<<.<<<<......>>>>>>>>>>>.>>....>>>>>>>>>>>>>..<<
.<..<<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>
>>.....>>>...<<<..<<.<<........>>>>.>>>....>>>>>>>
>>...<<.......>>......>>>>>>...>..>.>>..<<<<<<....
<.<<<....>>>.><<.<<<<<<<...<<<.<<<<<<<<<...<<<.>>>
...>>>>>>>...<<<<<<<<<.<......>>>>>>>>>>....>>>>>>
>>>>>>..>>..<<<<.<<<<..>>>>.>>>>....<<<......>>>..
.<<<<<<<...<<<(((.......<<<<<.....<<........<<<.<<
<<<<<<<<.......<<<<<.<<<...<<.........>>...>>>....
.....<<......>>...>>>>>...>>>>>>>>>>>>>..........)
)).>>...>>>>>..>>>...>>>>>>><<<<<<.<<<..........))
)).)))).................>>>>>>>>>....>>>>>>.<.<<<<
<<<<.<<......<<<<.<<<<....>>>>>>>>...>>>>>>>>>>..>
.....>>>>>><<.....<<.....<<<<<<.......>>>>>>.<<<..
...<<<<<.<<...<<<<<....<<.....>>..>>>>>....((<<<<<
<...<<....<<<....>>>.............<<<<..>>>>...>>..
..>>>..<<<<<........>>>>>))<<<<....<<<<<..<<<<<<<<
....>>>>>>>>..<<<....>>>...<..<<<<<<<...<<<<<<<<<<
<<.<<<<<<<<<<..[[[..>>>>>>....<<<<<.<<<<.<<<<.....
<<<.....>>>.....>>>>>>>>..>>>>>.....<<<<<<<.......
>>>>>>>>>>>...>>>>>>>>>>>>.........>>>>>>>..>.....
.....>>.>>>.>>>>.>>>..>>.....<<......>>>>>>>.<<<<.
....>>>>..>>>....<<<<.....<<<<<<<.<<..............
.<<<<<....<<..>>.....>>>>><<.<<<<<<<<<<<.<<<<<<<..
>>>>>>>......>>>>>..>>>>>>.>>............<<<<<<<<<
..<<<.........>>>..<<<<<<<....<<<<.....>>>>......>
>>>>>>..<....<<<..<<<<<<.<..<<<.....<<<<....>>>>..
...>>>>>>>>..>>>>>....>..>>.>>>>>>>.....>>.>>>>>>>
..>>>>>>....>>...<<<<<<......>>>>>>...<<<<<..<<<..
......<<<<<.<<<<<<..<<<<<.<<<<<<<<<.<<<<<.....>>>>
>...<<<<<.....<<<<................<<<<<<<<<<......
.>>>>>>>>>>..>>>>..>>>>>....>>>>>>>>>..>>>>>...<<.
>>..<<<<<<...........>>>>>>..<<...<<<<<<<...<<<<<<
.......>>>>>>.....<<.[.(((>>....>>>>>>>...<<<.<<<<
<.......>>>>.>>>>.<<<....>>>.>>)))].....<<<<<<<..<
<<..)))))).>>>>>>>>>>.........>>>>..>>>>>>>.......
<<<<<<...<<..>>...>>>>>>.....((...<<<<.<<<<<<..<.<
.<<<<<......>>>>>...>.>.<<<.....))..>>>.....>>>>>.
.>...>>>>......<<<<<<<....>>>.>>>>........>>>.>>>>
>.....<<<<<<.<..<<<..<.<<<<<.................>>>>>
.><<<<....<<<<....<<.<<<<<<<]]].>>>>>..>>.>>...>>>
>...>>>><<<<<...<<<<<...........>>>>>...>>>>>.>>>.
..>.>>>>>>.<<<<<<.<<..<<<<....<<<........<<<<<.<<<
<>>>>.>>>>>.......>>>...<<<..<<<<....<<<<.<<<<<<<.
.....>>>>>>>>>>>...>>>>..>>>..............>>>>..>>
>>>>>>.<<<<<....................<<.<<<<<<<.<<.....
<<<<....<<<<<......>>>>>...>>>>....>>....>>>.>>>>.
>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8fl9 Principles of human pre-60 S biogenesis.
• Resolution: 2.75 Å
• Binding residue (original residue number in PDB): G16 G18 M19 S34 K35 K38 K42 R50 W51 K53 R56 K61 R111 L112 N115 Q122
• Binding residue (residue number reindexed from 1): G16 G18 M19 S34 K35 K38 K42 R50 W51 K53 R56 K61 R111 L112 N115 Q122

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding

Biological Process

• GO:0006412 translation
• GO:0042254 ribosome biogenesis
• GO:0042273 ribosomal large subunit biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0005840 ribosome

External links

• PDB: RCSB:8fl9, PDBe:8fl9, PDBj:8fl9
• PDBsum: 8fl9
• PubMed: 37410842
• UniProt: Q9UHA3|RLP24_HUMAN Probable ribosome biogenesis protein RLP24 (Gene Name=RSL24D1)