Structure of PDB 7n30 Chain SQ Binding Site BS01

Receptor Information

>7n30 Chain SQ (length=80) Species: 83333 (Escherichia coli K-12)

KIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKLHVHDENNEC
GIGDVVEIRECRPLSKTKSWTLVRVVEKAV

Ligand information

>7n30 Chain 16 (length=1526)

aaauugaagaguuugaucauggcucagauugaacgcuggcggcaggccua
acacaugcaagucgaacgguaacaggaagaucuuugcugacgaguggcgg
acgggugaguaaugucugggaaacugccugauggagggggauaacuacug
gaaacgguagcuaauaccgcauaacgucgcaagaccaaagagggggaccu
ucgggccucuugccaucggaugugcccagaugggauuagcuaguaggugg
gguaacggcucaccuaggcgacgaucccuagcuggucugagaggaugacc
agccacacuggaacugagacacgguccagacuccuacgggaggcagcagu
ggggaauauugcacaaugggcgcaagccugaugcagccaugccgcgugua
ugaagaaggccuucggguuguaaaguacuuucagcggggaggaagggagu
aaaguuaauaccuuugcucauugacguuacccgcagaagaagcaccggcu
aacuccgugccagcagccgcgguaauacggagggugcaagcguuaaucgg
aauuacugggcguaaagcgcacgcaggcgguuuguuaagucagaugugaa
auccccgggcucaaccugggaacugcaucugauacuggcaagcuugaguc
ucguagagggggguagaauuccagguguagcggugaaaugcguagagauc
uggaggaauaccgguggcgaaggcggcccccuggacgaagacugacgcuc
aggugcgaaagcguggggagcaaacaggauuagauacccugguaguccac
gccguaaacgaugucgacuuggagguugugcccuugaggcguggcuuccg
gagcuaacgcguuaagucgaccgccuggggaguacggccgcaagguuaaa
acucaaaugaauugacgggggcccgcacaagcgguggagcaugugguuua
auucgaugcaacgcgaagaaccuuaccuggucuugacauccacggaaguu
uucagagaugagaaugugccuucgggaaccgugagacaggugcugcaugg
cugucgucagcucguguugugaaauguuggguuaagucccgcaacgagcg
caacccuuauccuuuguugccagcgguccggccgggaacucaaaggagac
ugccagugauaaacuggaggaagguggggaugacgucaagucaucauggc
ccuuacgaccagggcuacacacgugcuacaauggcgcauacaaagagaag
cgaccucgcgagagcaagcggaccucauaaagugcgucguaguccggauu
ggagucugcaacucgacuccaugaagucggaaucgcuaguaaucguggau
cagaaugccacggugaauacguucccgggccuuguacacaccgcccguca
caccaugggaguggguugcaaaagaaguagguagcuuaaccuucgggagg
gcgcuuaccacuuugugauucaugacuggggugaagucguaacaagguaa
ccguaggggaaccugcgguuggauca

........<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<..<.
.<<<.<<<..<<<..<<.<...<<<<<<<..>>>>.>>>.>..>>>>>..
....<<.......<<<<<<<..<<...<<<<<<<.<.<<.....<<<<<.
.....>>>>>......>>.>.....<<<....>>>....<<<<<<..<<.
...>>>>>>>>.>>>>>>>..>>.>>>>>>><<<....<<<..<<<<<<<
.........>>>>>>>>>>......>>>..<<<<<<<<....>>>>...>
>>>.>>.<<<<<.<.........>>>>>>.<<<<....>>>>...>>>>>
>>........<<<...<<<<<....>>>>.>>>>..>>.>>>>>>..<<<
<......<<<<....>>>>.....>>>>...<.<<<<<......<.<<<<
<<<<.......>>>>>>>>.>........>>>>>....>..<<<<<(((.
..<<<<<.....<<.)))>>.......>>>>>>>>>>..>>>>>>>>>..
........<<<((.....<<<<...<<<.<<<<<<<.<<<<<<<<<<...
...<<<<<<.....>>>>>>....>>>>>>>>..>>>>>>>>>...<<<<
<<<<...<<<<<<<....<<<<<<<<...<<<......>>>......>>>
>>>>>...........<<....>>.>>>>>>>..>>>.>>>>>...>>>.
..>>>>....<<<<<<...<<...<<<<.........>>>>...>>>>>>
>>..........<<<<<<.<<<<<<<<<<<<<.....>>>>>>>>>>>>>
..<<..))>>.....>>>>>>.>>>.<<<......<<<<....>>>>...
.>>>..)))).]<<<<<.<<<<<<<.<<.<<<<<<..<<<<<<<<<<...
...<<........>>..........<<<<<<<......<<<<<<<..<<<
<<<<....>>>>>>>...<<....>>..>>>>>.>>.<<<.<<<..<<<<
<<.......<<<<<<<<<....>>>..<<<<......>>>>..>>>>>>.
....<<<..<<<<<<<...<<..<<<.....>>>>>....>>>>>>>...
..<<<<<.....>>>>>.........>>>.........>>>...>>>>>>
>>>...>>>>>>>...>>.>>>>>>>>.....<<<<<<<.....<<<..<
<...<<<....>>>...>>....>>>.....>>>>>>>......<....<
<<<<<<........>>>>>>>....>.....>>>>>>....<<<<<<<..
.......>>>>>>>......>>...>>>>>>>>>>.>>....<..<<.<.
<<<<.<<<..<<<<<<<<<<<<....<<<<<<.<<<<..<<....>>.>>
>>>>>>>>...>>>>>>>>>>>>..>>>.>>>>..>.>>...>.....<<
<<<<<<<....>>>>>>>>>......

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7n30 Structural basis of early translocation events on the ribosome.
• Resolution: 2.66 Å
• Binding residue (original residue number in PDB): R6 S14 K16 M17 E18 K19 R27 Y34 K36 F37 R40 T41 T42 K43 L44 H45 E63 R65 P66 S68 K69 T70 K71 S72 W73
• Binding residue (residue number reindexed from 1): R3 S11 K13 M14 E15 K16 R24 Y31 K33 F34 R37 T38 T39 K40 L41 H42 E60 R62 P63 S65 K66 T67 K68 S69 W70

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0008270 zinc ion binding
• GO:0019843 rRNA binding
• GO:0070181 small ribosomal subunit rRNA binding

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0046677 response to antibiotic

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7n30, PDBe:7n30, PDBj:7n30
• PDBsum: 7n30
• PubMed: 34234344
• UniProt: P0AG63|RS17_ECOLI Small ribosomal subunit protein uS17 (Gene Name=rpsQ)