Structure of PDB 7wtl Chain SO Binding Site BS01

Receptor Information

>7wtl Chain SO (length=128) Species: 4932 (Saccharomyces cerevisiae)

NSQVFGVARIYASFNDTFVHVTDLSGKETIARVTGGMKVKADRDESSPYA
AMLAAQDVAAKCKEVGITAVHVKIRATGGTRTKTPGPGGQAALRALARSG
LRIGRIEDVTPVPSDSTRKKGGRRGRRL

Ligand information

>7wtl Chain C2 (length=1102)

uaucugguugauccugccaguagucauaugcuugucucaaagauuaagcc
augcaugucuaaguauaagcaaauacagugaaacugcgaauggcucauua
aaucaguuaucguuuauuugauaguuccucuacaugguauaacuguggua
auucuagagcuaauacaugcuuaaaaucucgacccuuuggaagagaugua
uuuauuagauaaaaaaucaaugucuucggacucuuugaugauucauaaua
acuuuucgaaucgcauggccuugugcuggcgaugguucauucaaauuucu
gcccuaucaacuuucgaugguaggauaguggccuaccaugguuucaacgg
guaacggggaauaaggguucgauuccggagagggagccugagaaacggcu
accacauccaaggaaggcagcaggcgcgcaaauuacccaauccuaauuca
gggagguagugacaauaaauaacgauacagggucuuguaauuggaaugag
uacaauguaaauaccuuaacgaggaacaauuggagggcaagucuggugcc
agcagccgcgguaauuccagcuccaauagcguauauuauguugcaguuaa
aaagcucguaguugaacuuugggcccgguuaacggggccuuuccuucugg
cuaaaccaggacuuuuacuuugaaaaaauuagaguguucaaagcaggcgu
auugcucgaauauauuagcauggaauaauagaauaggacguuugguucua
uuuuguugguuucuaggaccaucguaaugauuaauagggacggucggggg
caucaguauucaauugucagaggugaaauucuuggauuuauugaagacua
acuacugcgaaagcauuugccaaggacguuuucauuaaucaagaacgaaa
guuaggggaucgaagaugaucagauaccgucguagucuuaaccauaaacu
augccgacuagggaucgggugguguuuuuuuaaugacccacucggcaccu
uacgagaaaucaaagucuuuggguucuggggggaguauggucgcaaggcu
gaaacuuaaaggaauugagguuuccguaggugaaccugcggaaggaucau
ua

...<<<<<.{{[[[[>>>>>[[[..((((((................[[[
.[[[..<<....<<....<<.......>>...>>.>>......{{.....
...[[[..{{..{{....[[[...............<<....<<.<<<..
...>>>.>>.....>>.........<<<<<..<<....>>..>>>>>[..
.((((((......<<<<<<<<<<<....>>>....<<......>>)))))
).....]...<<<<..<<<.....>>>.>>>>....]]]...}}}}..]]
].<<<....<<<....<<<<<<<<.......>>>>>>>>>>>......>>
>...<<<.<<<<....>>>>....>>>.}}.<<.<<<..........>>>
.>>.<.<<<..>>>.>...]]]]]].........<<<....<<<.....>
>>..>>>...............<<<<<<<<....>>>>>.>>>......<
<..<...........>..>>.........<<<<<........<<<<.<<<
.<<....>>>>>....>>>>.>>>>>..)))))).]]].....[[[{{..
.....{{...[[[.{{....<<<<<<.<<..>>.>>>>>>....<<<<<<
.....>>>>>>...<<<<<.<<.......<<...<.......>..<<<..
...>>>....>>......>>.>>..>>>.........[[[..((((((((
....>>>>>>>>.)))))))).]]].....}}....<<<<<<.<<...<<
<<..<<..<<<<<<.<...<<<......>>>......>.>>>>>>..>>.
......<<....>>...>>>>...>>>>>.>>>...]]]...}}......
<<<<<<..<.<...<<<...........>>>...>>>>>>>>........
..<<<.<<.<<<..<<<<<<<<.<<<........>>>>>>>>>>>..>>>
...<<..}}>>...>>.....>>>.]]].<<<......<<<<....>>>>
....>>>..]]]].}}.....<<<<<<<<<....>>>>>>>>>.......
..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7wtl In vitro structural maturation of an early stage pre-40S particle coupled with U3 snoRNA release and central pseudoknot formation.
• Resolution: 3.3 Å
• Binding residue (original residue number in PDB): R18 N24 D25 F27 H29 G35 K36 E37 T38 R41 T43 G45 M46 D51 R52 R84 V121 P122 S123 D124 S125 T126 R127 K129 G130 G131 R132 R133
• Binding residue (residue number reindexed from 1): R9 N15 D16 F18 H20 G26 K27 E28 T29 R32 T34 G36 M37 D42 R43 R75 V112 P113 S114 D115 S116 T117 R118 K120 G121 G122 R123 R124

Gene Ontology

Molecular Function

• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0002181 cytoplasmic translation
• GO:0006364 rRNA processing
• GO:0006412 translation
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:0030686 90S preribosome
• GO:0032040 small-subunit processome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7wtl, PDBe:7wtl, PDBj:7wtl
• PDBsum: 7wtl
• PubMed: 36263816
• UniProt: P06367|RS14A_YEAST Small ribosomal subunit protein uS11A (Gene Name=RPS14A)