Structure of PDB 7n30 Chain SO Binding Site BS01

Receptor Information

>7n30 Chain SO (length=88) Species: 83333 (Escherichia coli K-12)

SLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFAEHKKDHH
SRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR

Ligand information

>7n30 Chain 16 (length=1526)

aaauugaagaguuugaucauggcucagauugaacgcuggcggcaggccua
acacaugcaagucgaacgguaacaggaagaucuuugcugacgaguggcgg
acgggugaguaaugucugggaaacugccugauggagggggauaacuacug
gaaacgguagcuaauaccgcauaacgucgcaagaccaaagagggggaccu
ucgggccucuugccaucggaugugcccagaugggauuagcuaguaggugg
gguaacggcucaccuaggcgacgaucccuagcuggucugagaggaugacc
agccacacuggaacugagacacgguccagacuccuacgggaggcagcagu
ggggaauauugcacaaugggcgcaagccugaugcagccaugccgcgugua
ugaagaaggccuucggguuguaaaguacuuucagcggggaggaagggagu
aaaguuaauaccuuugcucauugacguuacccgcagaagaagcaccggcu
aacuccgugccagcagccgcgguaauacggagggugcaagcguuaaucgg
aauuacugggcguaaagcgcacgcaggcgguuuguuaagucagaugugaa
auccccgggcucaaccugggaacugcaucugauacuggcaagcuugaguc
ucguagagggggguagaauuccagguguagcggugaaaugcguagagauc
uggaggaauaccgguggcgaaggcggcccccuggacgaagacugacgcuc
aggugcgaaagcguggggagcaaacaggauuagauacccugguaguccac
gccguaaacgaugucgacuuggagguugugcccuugaggcguggcuuccg
gagcuaacgcguuaagucgaccgccuggggaguacggccgcaagguuaaa
acucaaaugaauugacgggggcccgcacaagcgguggagcaugugguuua
auucgaugcaacgcgaagaaccuuaccuggucuugacauccacggaaguu
uucagagaugagaaugugccuucgggaaccgugagacaggugcugcaugg
cugucgucagcucguguugugaaauguuggguuaagucccgcaacgagcg
caacccuuauccuuuguugccagcgguccggccgggaacucaaaggagac
ugccagugauaaacuggaggaagguggggaugacgucaagucaucauggc
ccuuacgaccagggcuacacacgugcuacaauggcgcauacaaagagaag
cgaccucgcgagagcaagcggaccucauaaagugcgucguaguccggauu
ggagucugcaacucgacuccaugaagucggaaucgcuaguaaucguggau
cagaaugccacggugaauacguucccgggccuuguacacaccgcccguca
caccaugggaguggguugcaaaagaaguagguagcuuaaccuucgggagg
gcgcuuaccacuuugugauucaugacuggggugaagucguaacaagguaa
ccguaggggaaccugcgguuggauca

........<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<..<.
.<<<.<<<..<<<..<<.<...<<<<<<<..>>>>.>>>.>..>>>>>..
....<<.......<<<<<<<..<<...<<<<<<<.<.<<.....<<<<<.
.....>>>>>......>>.>.....<<<....>>>....<<<<<<..<<.
...>>>>>>>>.>>>>>>>..>>.>>>>>>><<<....<<<..<<<<<<<
.........>>>>>>>>>>......>>>..<<<<<<<<....>>>>...>
>>>.>>.<<<<<.<.........>>>>>>.<<<<....>>>>...>>>>>
>>........<<<...<<<<<....>>>>.>>>>..>>.>>>>>>..<<<
<......<<<<....>>>>.....>>>>...<.<<<<<......<.<<<<
<<<<.......>>>>>>>>.>........>>>>>....>..<<<<<(((.
..<<<<<.....<<.)))>>.......>>>>>>>>>>..>>>>>>>>>..
........<<<((.....<<<<...<<<.<<<<<<<.<<<<<<<<<<...
...<<<<<<.....>>>>>>....>>>>>>>>..>>>>>>>>>...<<<<
<<<<...<<<<<<<....<<<<<<<<...<<<......>>>......>>>
>>>>>...........<<....>>.>>>>>>>..>>>.>>>>>...>>>.
..>>>>....<<<<<<...<<...<<<<.........>>>>...>>>>>>
>>..........<<<<<<.<<<<<<<<<<<<<.....>>>>>>>>>>>>>
..<<..))>>.....>>>>>>.>>>.<<<......<<<<....>>>>...
.>>>..)))).]<<<<<.<<<<<<<.<<.<<<<<<..<<<<<<<<<<...
...<<........>>..........<<<<<<<......<<<<<<<..<<<
<<<<....>>>>>>>...<<....>>..>>>>>.>>.<<<.<<<..<<<<
<<.......<<<<<<<<<....>>>..<<<<......>>>>..>>>>>>.
....<<<..<<<<<<<...<<..<<<.....>>>>>....>>>>>>>...
..<<<<<.....>>>>>.........>>>.........>>>...>>>>>>
>>>...>>>>>>>...>>.>>>>>>>>.....<<<<<<<.....<<<..<
<...<<<....>>>...>>....>>>.....>>>>>>>......<....<
<<<<<<........>>>>>>>....>.....>>>>>>....<<<<<<<..
.......>>>>>>>......>>...>>>>>>>>>>.>>....<..<<.<.
<<<<.<<<..<<<<<<<<<<<<....<<<<<<.<<<<..<<....>>.>>
>>>>>>>>...>>>>>>>>>>>>..>>>.>>>>..>.>>...>.....<<
<<<<<<<....>>>>>>>>>......

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7n30 Structural basis of early translocation events on the ribosome.
• Resolution: 2.66 Å
• Binding residue (original residue number in PDB): T8 N20 D21 T22 G23 S24 Q28 Q35 H38 L39 H42 H46 K48 D49 H51 S52 R54 G55 S61 K65 Y69
• Binding residue (residue number reindexed from 1): T7 N19 D20 T21 G22 S23 Q27 Q34 H37 L38 H41 H45 K47 D48 H50 S51 R53 G54 S60 K64 Y68

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding
• GO:0070181 small ribosomal subunit rRNA binding

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0006417 regulation of translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7n30, PDBe:7n30, PDBj:7n30
• PDBsum: 7n30
• PubMed: 34234344
• UniProt: P0ADZ4|RS15_ECOLI Small ribosomal subunit protein uS15 (Gene Name=rpsO)