Structure of PDB 7wtq Chain SL Binding Site BS01

Receptor Information

>7wtq Chain SL (length=146) Species: 4932 (Saccharomyces cerevisiae)

STELTVQSERAFQKQPHIFNNPKVKTSKRTKRWYKNAGLGFKTPKTAIEG
SYIDKKCPFTGLVSIRGKILTGTVVSTKMHRTIVIRRAYLHYIPKYNRYE
KRHKNVPVHVSPAFRVQVGDIVTVGQCRPISKTVRFNVVKVSAAAG

Ligand information

>7wtq Chain C2 (length=1255)

uaucugguugauccugccaguagucauaugcuugucucaaagauuaagcc
augcaugucuaaguauaagcaaauacagugaaacugcgaauggcucauua
aaucaguuaucguuuauuugauaguuccucuacaugguauaacuguggua
auucuagagcuaauacaugcuuaaaaucucgacccuuuggaagagaugua
uuuauuagauaaaaaaucaaugucuucggacucuuugaugauucauaaua
acuuuucgaaucgcauggccuugugcuggcgaugguucauucaaauuucu
gcccuaucaacuuucgaugguaggauaguggccuaccaugguuucaacgg
guaacggggaauaaggguucgauuccggagagggagccugagaaacggcu
accacauccaaggaaggcagcaggcgcgcaaauuacccaauccuaauuca
gggagguagugacaauaaauaacgauacagggucuuguaauuggaaugag
uacaauguaaauaccuuaacgaggaacaauuggagggcaagucuggugcc
agcagccgcgguaauuccagcuccaauagcguauauuaaaguuguugcag
uuaaaaagcucguaguugaacuuugggcccgguuaacggggccuuuccuu
cuggcuaaaccaggacuuuuacuuugaaaaaauuagaguguucaaagcag
gcguauugcucgaauauauuagcauggaauaauagaauaggacguuuggu
ucuauuuuguugguuucuaggaccaucguaaugauuaauagggacggucg
ggggcaucaguauucaauugucagaggugaaauucuuggauuuauugaag
acuaacuacugcgaaagcauuugccaaggacguuuucauuaaucaagaac
gaaaguuaggggaucgaagaugaucagauaccgucguagucuuaaccaua
aacuaugccgacuagggaucgggugguguuuuuuuaaugacccacucggc
accuuacgagaaaucaaagucuuuggguucuggggggaguauggucgcaa
ggcugaaacuuaaagaauugacggaagggcgcccuuuguaccgcccgucg
cuaguaccgauugaauggcuuagugaggccucaggaucugcuuagagaag
ggggcaacuccaucucagagcggagaauuuggacaaacuuggucauuuag
aggaacuaaaagucguaacaagguuuccguaggugaaccugcggaaggau
cauua

...<<<<<.[[[[.{>>>>><<<<.<<<<<<...<.<...<......<<<
.<<<..<<....<<....<<.......>>...>>.>>......<<.....
...<<<..<<..<<....<<<...............<<....<<.<<<..
...>>>.>>.....>>.........<<<<<..<<....>>..>>>>><..
.<<<<<<......(((((((.<<<....>>>..............>>>>>
>.....>...<<<<..<<<.....>>>.>>>>....>>>...>>>>..>>
>.<<<....<<<....<<<<<<<<.......>>>>>>>>>>>......>>
>...<<<.<<<<....>>>>....>>>.>>.<<.<<<..........>>>
.>>.<.<<<..>>>.>...>>>>>>.........<<<....<<<.....>
>>..>>>..>....>.>.....<<<<<<<<....>>>>>.>>>......<
<..<...........>..>>.........<<<<<........<<<<.<<<
.<<....>>>>>....>>>>.>>>>>..>>>>>>>>>>.........<<<
[[.....<.<<...<<<.<<....<<<<<<.<<..>>.>>>>>>....<<
<<<<.....>>>>>>...<<<<<.<<.......<<...<.......>..<
<<.....>>>....>>......>>.>>..>>>.........<<<..<<<<
<<<<.....))))))).>>>>>>>>.>>>.....>>....<<<<<<.<<.
..<<<<..<<..<<<<<<.<...<<<..(...>>>......>.>>>>>>.
.>>.......<<....>>...>>>>...>>>>>.>>>...>>>...>>.>
....<<<<<<<...<...<<<<..)......>>>>...>>>>>>>>....
......<<<.<<.<<<..<<<<<<<<.<<<........>>>>>>>>>>>.
.>>>...<<..]]>>...>>.....>>>.>>>.<<<......<<<<....
>>>>....>>>..}]].]]..<<.<<<<<..>>>>>>>.<<<...<<.<.
.<<<<.<<..<<<<<<<<<<.<<<..<.<<..<...<<<<<<<.<<<<.<
<<<....>>>>.>>>>.>>>>>>>...>..>>.>..>>>.>>>>>>>>>>
.>>.>>>>...>.>>......>>><<<<<<<<<....>>>>>>>>>....
.....

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7wtq The nucleoplasmic phase of pre-40S formation prior to nuclear export.
• Resolution: 3.7 Å
• Binding residue (original residue number in PDB): E10 Q14 P17 H18 F20 W34 K36 N37 G39 L40 K57 L63 S65 I66 R67 K69 K79 M80 Y90 H92 K96 Y97 R103 H104 K105 N106 Q127 R129 P130 I131 S132 K133 T134 R136 F137
• Binding residue (residue number reindexed from 1): E9 Q13 P16 H17 F19 W33 K35 N36 G38 L39 K56 L62 S64 I65 R66 K68 K78 M79 Y89 H91 K95 Y96 R102 H103 K104 N105 Q126 R128 P129 I130 S131 K132 T133 R135 F136

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:0030686 90S preribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7wtq, PDBe:7wtq, PDBj:7wtq
• PDBsum: 7wtq
• PubMed: 36321656
• UniProt: P0CX47|RS11A_YEAST Small ribosomal subunit protein uS17A (Gene Name=RPS11A)