Structure of PDB 7n30 Chain SK Binding Site BS01

Receptor Information

>7n30 Chain SK (length=117) Species: 83333 (Escherichia coli K-12)

RKQVSDGVAHIHASFNNTIVTITDRQGNALGWATAGGSGFRGSRKSTPFA
AQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNAAGFRITNITD
VTPIPHNGCRPPKKRRV

Ligand information

>7n30 Chain 16 (length=1526)

aaauugaagaguuugaucauggcucagauugaacgcuggcggcaggccua
acacaugcaagucgaacgguaacaggaagaucuuugcugacgaguggcgg
acgggugaguaaugucugggaaacugccugauggagggggauaacuacug
gaaacgguagcuaauaccgcauaacgucgcaagaccaaagagggggaccu
ucgggccucuugccaucggaugugcccagaugggauuagcuaguaggugg
gguaacggcucaccuaggcgacgaucccuagcuggucugagaggaugacc
agccacacuggaacugagacacgguccagacuccuacgggaggcagcagu
ggggaauauugcacaaugggcgcaagccugaugcagccaugccgcgugua
ugaagaaggccuucggguuguaaaguacuuucagcggggaggaagggagu
aaaguuaauaccuuugcucauugacguuacccgcagaagaagcaccggcu
aacuccgugccagcagccgcgguaauacggagggugcaagcguuaaucgg
aauuacugggcguaaagcgcacgcaggcgguuuguuaagucagaugugaa
auccccgggcucaaccugggaacugcaucugauacuggcaagcuugaguc
ucguagagggggguagaauuccagguguagcggugaaaugcguagagauc
uggaggaauaccgguggcgaaggcggcccccuggacgaagacugacgcuc
aggugcgaaagcguggggagcaaacaggauuagauacccugguaguccac
gccguaaacgaugucgacuuggagguugugcccuugaggcguggcuuccg
gagcuaacgcguuaagucgaccgccuggggaguacggccgcaagguuaaa
acucaaaugaauugacgggggcccgcacaagcgguggagcaugugguuua
auucgaugcaacgcgaagaaccuuaccuggucuugacauccacggaaguu
uucagagaugagaaugugccuucgggaaccgugagacaggugcugcaugg
cugucgucagcucguguugugaaauguuggguuaagucccgcaacgagcg
caacccuuauccuuuguugccagcgguccggccgggaacucaaaggagac
ugccagugauaaacuggaggaagguggggaugacgucaagucaucauggc
ccuuacgaccagggcuacacacgugcuacaauggcgcauacaaagagaag
cgaccucgcgagagcaagcggaccucauaaagugcgucguaguccggauu
ggagucugcaacucgacuccaugaagucggaaucgcuaguaaucguggau
cagaaugccacggugaauacguucccgggccuuguacacaccgcccguca
caccaugggaguggguugcaaaagaaguagguagcuuaaccuucgggagg
gcgcuuaccacuuugugauucaugacuggggugaagucguaacaagguaa
ccguaggggaaccugcgguuggauca

........<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<..<.
.<<<.<<<..<<<..<<.<...<<<<<<<..>>>>.>>>.>..>>>>>..
....<<.......<<<<<<<..<<...<<<<<<<.<.<<.....<<<<<.
.....>>>>>......>>.>.....<<<....>>>....<<<<<<..<<.
...>>>>>>>>.>>>>>>>..>>.>>>>>>><<<....<<<..<<<<<<<
.........>>>>>>>>>>......>>>..<<<<<<<<....>>>>...>
>>>.>>.<<<<<.<.........>>>>>>.<<<<....>>>>...>>>>>
>>........<<<...<<<<<....>>>>.>>>>..>>.>>>>>>..<<<
<......<<<<....>>>>.....>>>>...<.<<<<<......<.<<<<
<<<<.......>>>>>>>>.>........>>>>>....>..<<<<<(((.
..<<<<<.....<<.)))>>.......>>>>>>>>>>..>>>>>>>>>..
........<<<((.....<<<<...<<<.<<<<<<<.<<<<<<<<<<...
...<<<<<<.....>>>>>>....>>>>>>>>..>>>>>>>>>...<<<<
<<<<...<<<<<<<....<<<<<<<<...<<<......>>>......>>>
>>>>>...........<<....>>.>>>>>>>..>>>.>>>>>...>>>.
..>>>>....<<<<<<...<<...<<<<.........>>>>...>>>>>>
>>..........<<<<<<.<<<<<<<<<<<<<.....>>>>>>>>>>>>>
..<<..))>>.....>>>>>>.>>>.<<<......<<<<....>>>>...
.>>>..)))).]<<<<<.<<<<<<<.<<.<<<<<<..<<<<<<<<<<...
...<<........>>..........<<<<<<<......<<<<<<<..<<<
<<<<....>>>>>>>...<<....>>..>>>>>.>>.<<<.<<<..<<<<
<<.......<<<<<<<<<....>>>..<<<<......>>>>..>>>>>>.
....<<<..<<<<<<<...<<..<<<.....>>>>>....>>>>>>>...
..<<<<<.....>>>>>.........>>>.........>>>...>>>>>>
>>>...>>>>>>>...>>.>>>>>>>>.....<<<<<<<.....<<<..<
<...<<<....>>>...>>....>>>.....>>>>>>>......<....<
<<<<<<........>>>>>>>....>.....>>>>>>....<<<<<<<..
.......>>>>>>>......>>...>>>>>>>>>>.>>....<..<<.<.
<<<<.<<<..<<<<<<<<<<<<....<<<<<<.<<<<..<<....>>.>>
>>>>>>>>...>>>>>>>>>>>>..>>>.>>>>..>.>>...>.....<<
<<<<<<<....>>>>>>>>>......

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7n30 Structural basis of early translocation events on the ribosome.
• Resolution: 2.66 Å
• Binding residue (original residue number in PDB): H22 N28 N29 T33 G39 N40 A41 W44 T46 G48 G49 K57 K87 P115 I116 P117 H118 N119 G120 C121 R122 P124 K125 R127 R128 V129
• Binding residue (residue number reindexed from 1): H10 N16 N17 T21 G27 N28 A29 W32 T34 G36 G37 K45 K75 P103 I104 P105 H106 N107 G108 C109 R110 P112 K113 R115 R116 V117

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding
• GO:0070181 small ribosomal subunit rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7n30, PDBe:7n30, PDBj:7n30
• PDBsum: 7n30
• PubMed: 34234344
• UniProt: P0A7R9|RS11_ECOLI Small ribosomal subunit protein uS11 (Gene Name=rpsK)