Structure of PDB 7cpu Chain SG Binding Site BS01

Receptor Information

>7cpu Chain SG (length=204) Species: 10090 (Mus musculus)

MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVV
RISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGC
IVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLDVR
QYVVRKPLNKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTKKNKE
EAAE

Ligand information

>7cpu Chain S2 (length=1656)

uaccugguugauccugccaguagcauaugcuugucucaaagauuaagcca
ugcaugucuaaguacgcacggccgguacagugaaacugcgaauggcucau
uaaaucaguuaugguuccuuuggucgcucacuuggauaacugugguaauu
cuagagcuaauacaugccgacgggcgcauuuaucaguggugacucuagau
aaccgccgaucgcacgcccuggcggcgacgacccauucgaacgucugccc
uaucaacuuucgaugguagucgccgugccuaccauggugaccacggguga
cggggaaucaggguucgauuccggagagggagccugagaaacggcuacca
cauccaaggaaggcagcaggcgcgcaaauuacccacucccgacccgggga
gguagugacgaaaaauaacaauacaggacucuuucgaggcccuguaauug
gaaugaguccacuuuaaauccuuuaacgaggauccauuggagggcaaguc
uggugccagcagccgcgguaauuccagcuccaauagcguauauuaaaguu
gcugcaguuaaaaagcucguaguuggaucuucucgaagcguuuacuuuga
aaaaauuagaguguucaaagcaggcccgagccgccuggauaccgcagcua
ggaauaauggaauaggaccgcgguucuauuuuguugguuuucggaacuga
ggccaugauuaagagggacggccgggggcauucguauugcgccgcuagag
gugaaauucuuggaccggcgcaagacggaccagagcgaaagcauuugcca
agaauguuuucauuaaucaagaacgaaagucggagguucgaagacgauca
gauaccgucguaguuccgaccauaaacgaugccgacuggcgaugcggcgg
cguuauucccaugacccgccgggcagcuuccgggaaaccaaagucuuugg
guuccggggggaguaugguugcaaagcugaaacuuaaaggaauugacgga
agggcaccaccaggaguggagccugcggcuuaauuugacucaacacggga
aaccucacccggcccggacacggacaggauugacagauugauagcucuuu
cucgauuccgugggugguggugcauggccguucuuaguugguggagcgau
uugucugguuaauuccgauaacgaacgagacucuggcaugcuaacuaguu
acgcgacccccgagcggucggcguaacuucuuagagggacaaguggcguu
cagccacccgagauugagcaauaacaggucugugaugcccuuagaugucc
ggggcugcacgcgcgcuacacugacuggcucagcgugugccuacccugcg
ccggcaggcgcggguaacccguugaaccccauucgugauggggaucgggg
auugcaauuauuccccaugaacgaggaauucccaguaagugcgggucaua
agcuugcguugauuaagucccugcccuuuguacacaccgcccgucgcuac
uaccgauuggaugguuuagugaggcccucggaucggccccgccggggucg
gcccacggcccuggcggagcgcugagaagacggucgaacuugacuaucua
gaggaaguaaaagucguaacaagguuuccguaggugaaccugcggaagga
ucauua

...<<<<<.[.((((>>>>><<<.<<<<<<...<.<<..<......<<<.
<<<..<<....<<....<<..........>>...>>.>>......<<..<
.....<<<......<<....<<<.........<<.....<<.<<......
.>>.>>......>>...........<<..<<<<<...<......>..>>>
>>..>>...<<<<.<<<<..>>>>>>>>....>>>...>>....>>>.<<
<....<<<....<<<<<<<.........>>>>>>>>>>......>>>...
<<<.<<<<....>>>>....>>>>>>.<<.<<<..........>>>.>>.
<.<<....>>.>...>>>>>>.........<<<....<<<<...>>>>..
>>>..>...>>.>.....<<.<<<<<<............>>.>>>.>.>>
......<<..<...........>..>>.........<<<<<((......<
<<<.....<<..))>>.......>>>>.>>>>>..>>>>>>.>>>.....
....<.<((.....<.<<...<<<.<<...<.....>....<<<<<<.<.
......<<...<.......>..<<<.......>>>....>>......>.>
>>..>>>........<.<<.<<.........................>>.
>>.>....>>....<<<<<<..<...<<<<..<<..<<<<<<<<...<<<
......>>>......>>>>>>>>..>>.......<<....>>...>>>>.
.>..>>>.>>>...>>>...>>.>....<<<<<<<...<...<<<<.<..
...>.>>>>...>>>>>>>>..........<<<.<<.<<<..<.<<<<<<
.<<<<......>>>>>>>>>>.>..>>>...<<..))>>...>>.....>
>>.>.>.<<<......<<<<....>>>>....>>>..)))).]<<<<<.<
<<<<<<..<<..<<<<<..<<<.<<<<........<<........>>...
.......<<<<<.<....<<<<<<.......<<.<<<........>>>.>
>.....>>>>>>...<<.<<<..<<<<<<<<<....<<<.<<<<<....>
>>...<<<......>>>...>>.>>>....<<<...<...<<<<..<<<<
<<<<<<<<.......>>>>.>>>>>>>>..>>>>..>.....<<<<<...
..>>>>>........>>>....>>>.>>>...>.>>>>>>>.....>.>>
>>>......>>>>.>>>.....<<.<<<........<.......<<<<<<
<<....>>>>>>>>....>.........>>>.>>......<.....<<<<
<..........>>>>>.....>.....>>>>>.....<<<<<<<<.....
..>>>>>>>>......>>...>>>>>>>>>>.>>....<..<<.<..<<<
<.<<....<<<<<<<<.<<<..<<<<.<<...<<<<<.............
....................>>>>>...>>.>>>>..>>>.>>>>>>>>.
..>>.>>>>...>.>>...>.....<<<<<<<<<<..>>>>>>>>>>...
......

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7cpu A male germ-cell-specific ribosome controls male fertility.
• Resolution: 2.82 Å
• Binding residue (original residue number in PDB): K2 N4 P8 Q13 K14 L15 R31 A33 S53 N56 Q59 G60 F61 P62 Q65 G66 V67 L68 R72 R74 L75 L76 C83 Y84 R85 R87 R88 G90 R92 K93 R94 S96 S107 V108 R131 R132 L133 G134 P135 K136 R137 S139 R140 K143 R154 K160 L162 K167 K168 R170 K172 P174 K175 I176 Q177 R178 R183 L185 Q186 H187 K188 R189 R190 I192 A193 K195 K196 R198
• Binding residue (residue number reindexed from 1): K2 N4 P8 Q13 K14 L15 R31 A33 S53 N56 Q59 G60 F61 P62 Q65 G66 V67 L68 R72 R74 L75 L76 C83 Y84 R85 R87 R88 G90 R92 K93 R94 S96 S107 V108 R131 R132 L133 G134 P135 K136 R137 S139 R140 K143 R150 K156 L158 K163 K164 R166 K168 P170 K171 I172 Q173 R174 R179 L181 Q182 H183 K184 R185 R186 I188 A189 K191 K192 R194

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019901 protein kinase binding

Biological Process

• GO:0000082 G1/S transition of mitotic cell cycle
• GO:0000278 mitotic cell cycle
• GO:0001890 placenta development
• GO:0002181 cytoplasmic translation
• GO:0002309 T cell proliferation involved in immune response
• GO:0006364 rRNA processing
• GO:0006412 translation
• GO:0006924 activation-induced cell death of T cells
• GO:0007369 gastrulation
• GO:0008284 positive regulation of cell population proliferation
• GO:0022605 mammalian oogenesis stage
• GO:0031929 TOR signaling
• GO:0033077 T cell differentiation in thymus
• GO:0042274 ribosomal small subunit biogenesis
• GO:0042593 glucose homeostasis
• GO:0043065 positive regulation of apoptotic process
• GO:0043066 negative regulation of apoptotic process
• GO:0048821 erythrocyte development

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005783 endoplasmic reticulum
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0015935 small ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:0030425 dendrite
• GO:0032040 small-subunit processome
• GO:0036464 cytoplasmic ribonucleoprotein granule
• GO:0044297 cell body
• GO:0048471 perinuclear region of cytoplasm
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7cpu, PDBe:7cpu, PDBj:7cpu
• PDBsum: 7cpu
• PubMed: 36517592
• UniProt: P62754|RS6_MOUSE Small ribosomal subunit protein eS6 (Gene Name=Rps6)