Structure of PDB 7qvp Chain SE Binding Site BS01

Receptor Information

>7qvp Chain SE (length=262) Species: 9606 (Homo sapiens)

ARGPKKHLKRVAAPKHWMLDKLTGVFAPRPSTGPHKLRECLPLIIFLRNR
LKYALTGDEVKKICMQRFIKIDGKVRTDITYPAGFMDVISIDKTGENFRL
IYDTKGRFAVHRITPEEAKYKLCKVRKIFVGTKGIPHLVTHDARTIRYPD
PLIKVNDTIQIDLETGKITDFIKFDTGNLCMVTGGANLGRIGVITNRERH
PGSFDVVHVKDANGNSFATRLSNIFVIGKGNKPWISLPRGKGIRLTIAEE
RDKRLAAKQSSG

Ligand information

>7qvp Chain S2 (length=1714)

uaccugguugauccugccaguagcauaugcuugucucaaagauuaagcca
ugcaugucuaaguacgcacggccgguacagugaaacugcgaauggcucau
uaaaucaguuaugguuccuuuggucgcucgcacuuggauaacugugguaa
uucuagagcuaauacaugccgacgggcgcugacccccuucgcggggggga
ugcgugcauuuaucaguggugacucuagauaaccucgggccgaucgcacg
ccccccguggcggcgacgacccauucgaacgucugcccuaucaacuuucg
augguagucgccgugccuaccauggugaccacgggugacggggaaucagg
guucgauuccggagagggagccugagaaacggcuaccacauccaaggaag
gcagcaggcgcgcaaauuacccacucccgacccggggagguagugacgaa
aaauaacaauacaggacucuuucgaggcccuguaauuggaaugaguccac
uuuaaauccuuuaacgaggauccauuggagggcaagucuggugccagcag
ccgcgguaauuccagcuccaauagcguauauuaaaguugcugcaguuaaa
aagcucguaguuggaucuugggagcgggcguccccgccccucucggcgcc
cgaagcguuuacuuugaaaaaauuagaguguucaaagcaggcccgagccg
ccuggauaccgcagcuaggaauaauggaauaggaccgcgguucuauuuug
uugguuuucggaacugaggccaugauuaagagggacggccgggggcauuc
guauugcgccgcuagaggugaaauucuuggaccggcgcaagacggaccag
agcgaaagcauuugccaagaauguuuucauuaaucaagaacgaaagucgg
agguucgaagacgaucagauaccgucguaguuccgaccauaaacgaugcc
gaccggcgaugcggcggcguuauucccaugacccgccgggcagcuuccgg
gaaaccaaagucuuuggguuccggggggaguaugguugcaaagcugaaac
uuaaaggaauugacggaagggcaccaccaggaguggagccugcggcuuaa
uuugacucaacacgggaaaccucacccggcccggacacggacaggauuga
cagauugauagcucuuucucgauuccgugggugguggugcauggccguuc
uuaguugguggagcgauuugucugguuaauuccgauaacgaacgagacuc
uggcaugcuaacuaguuacgcgacccccgagcggucggcguccaacuucu
uagagggacaaguggcguucagccacccgagauugagcaauaacaggucu
gugaugcccuuagauguccggggcugcacgcgcgcuacacugacuggcuc
agcgugugccuacccuacgccggcaggcgcggguaacccguugaacccca
uucgugauggggaucggggauugcaauuauuccccaugaacgaggaauuc
ccaguaagugcgggucauaagcuugcguugauuaagucccugcccuuugu
acacaccgcccgucgcuacuaccgauuggaugguuuagugaggcccucgg
aucggccccgccggggcccuggcggagcgcugagaagacggucgaacuug
acuaucuagaggaaguaaaagucguaacaagguuuccguaggugaaccug
cggaaggaucauua

...<<<<<.[.((((>>>>><<<.<<<<<<...<.<<..<......<<<.
<<<..<<....<<....<<..........>>...>>.>>......<<...
.....<<<.<....<<....<<<<<.........<<.....<<.<<<...
..>>>.>>......>>.........<<<<...<<<<<......>>>>>..
.>>>><<..<<<<<...<......>..>>>>>......>>...<<<<.<<
<<......>>>>>>>>.>>.>>>...>>..>.>>>.<<<....<<<....
<<<<<<<.........>>>>>>>>>>......>>>...<<<.<<<<....
>>>>....>>>.>>.<<.<<<..........>>>.>>.<.<<....>>.>
...>>>>>>.........<<<....<<<<...>>>>..>>>..>...>>.
>.....<<.<<<<<<............>>.>>>>..>>......<<..<.
..........>..>>.........<<<<<((......<<<<.....<<..
))>>.......>>>>.>>>>>..>>>>>>.>>>.........<.<((...
..<.<<...<<<.<<...................................
........<<<<<<.<.......<<...<.......>.<<<<.......>
>>>...>>......>.>>>..>>>........<.<<.<<<<<<<......
.........>>>>>>>.>>.>....>>....<<<<<<..<...<<<<..<
<..<<<<<<<<...<<<......>>>......>>>>>>>>..>>......
.<<....>>...>>>>..>..>>>.>>>...>>>...>>.>....<<<<<
<<...<...<<<<.<.....>.>>>>...>>>>>>>>..........<<<
.<<.<<<..<.<<<<<<.<<<<......>>>>>>>>>>.>..>>>...<<
..))>>...>>.....>>>.>.>.<<<......<<<<....>>>>....>
>>..)))).]<<<<<.<<<<<<<..<<..<<<<<..<<<.<<<<<<....
..<<........>>..........<<<<<......<<<<<<.......<<
.<<<........>>>.>>.....>>>>>>.<.<<.<<<..<<.<<<<<<.
...<<<.<<<<<....>>>...<<<......>>>...>>.>>>....<<<
...<...<<<<..<<<<<<<<<<<<.......>>>>.>>>>..>>>>..>
>>>..>.....<<<<<.....>>>>>........>>>....>>>.>>>..
...>>>>>>>>......>>>>>...>>.>>>>.>>>.....<<.<<<...
.....<.......<<<.<<<<....>>>>.>>>....>.........>>>
.>>......<.....<<<<<..........>>>>>.....>.....>>>>
>.....<<<<<<<<.......>>>>>>>>......>>...>>>>>>>>>>
.>>....<..<<.<..<<<<.<<....<<<<<<<<.<<<..<<<<.<<..
.<<<<<.<....<.....>.....>...>>>>>...>>.>>>>..>>>.>
>>>>>>>...>>.>>>>...>.>>...>.....<<<<<<<<<<..>>>>>
>>>>>.........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7qvp A distinct mammalian disome collision interface harbors K63-linked polyubiquitination of uS10 to trigger hRQT-mediated subunit dissociation.
• Resolution: 3.0 Å
• Binding residue (original residue number in PDB): A2 R3 G4 K6 K7 H8 K10 V12 M19 D21 K22 L23 F27 A28 P29 R30 T33 G34 K37 L38 R49 T57 D59 K62 K63 M66 K75 R77 Y82 K106 R108 K128 V131 G132 K134 A144 T146 R148 K155 N188 E199 H201 G203 A219
• Binding residue (residue number reindexed from 1): A1 R2 G3 K5 K6 H7 K9 V11 M18 D20 K21 L22 F26 A27 P28 R29 T32 G33 K36 L37 R48 T56 D58 K61 K62 M65 K74 R76 Y81 K105 R107 K127 V130 G131 K133 A143 T145 R147 K154 N187 E198 H200 G202 A218

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0008284 positive regulation of cell population proliferation
• GO:0042274 ribosomal small subunit biogenesis
• GO:0045727 positive regulation of translation

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0005925 focal adhesion
• GO:0015935 small ribosomal subunit
• GO:0016020 membrane
• GO:0022626 cytosolic ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:0032040 small-subunit processome
• GO:0036464 cytoplasmic ribonucleoprotein granule
• GO:0045202 synapse
• GO:0070062 extracellular exosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7qvp, PDBe:7qvp, PDBj:7qvp
• PDBsum: 7qvp
• PubMed: 36302773
• UniProt: P62701|RS4X_HUMAN Small ribosomal subunit protein eS4, X isoform (Gene Name=RPS4X)