Structure of PDB 7n30 Chain SE Binding Site BS01

Receptor Information

>7n30 Chain SE (length=155) Species: 83333 (Escherichia coli K-12)

LQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNGRVGFGYGKAREVPAAI
QKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEGTGIIAGGA
MRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
VEEIL

Ligand information

>7n30 Chain 16 (length=1526)

aaauugaagaguuugaucauggcucagauugaacgcuggcggcaggccua
acacaugcaagucgaacgguaacaggaagaucuuugcugacgaguggcgg
acgggugaguaaugucugggaaacugccugauggagggggauaacuacug
gaaacgguagcuaauaccgcauaacgucgcaagaccaaagagggggaccu
ucgggccucuugccaucggaugugcccagaugggauuagcuaguaggugg
gguaacggcucaccuaggcgacgaucccuagcuggucugagaggaugacc
agccacacuggaacugagacacgguccagacuccuacgggaggcagcagu
ggggaauauugcacaaugggcgcaagccugaugcagccaugccgcgugua
ugaagaaggccuucggguuguaaaguacuuucagcggggaggaagggagu
aaaguuaauaccuuugcucauugacguuacccgcagaagaagcaccggcu
aacuccgugccagcagccgcgguaauacggagggugcaagcguuaaucgg
aauuacugggcguaaagcgcacgcaggcgguuuguuaagucagaugugaa
auccccgggcucaaccugggaacugcaucugauacuggcaagcuugaguc
ucguagagggggguagaauuccagguguagcggugaaaugcguagagauc
uggaggaauaccgguggcgaaggcggcccccuggacgaagacugacgcuc
aggugcgaaagcguggggagcaaacaggauuagauacccugguaguccac
gccguaaacgaugucgacuuggagguugugcccuugaggcguggcuuccg
gagcuaacgcguuaagucgaccgccuggggaguacggccgcaagguuaaa
acucaaaugaauugacgggggcccgcacaagcgguggagcaugugguuua
auucgaugcaacgcgaagaaccuuaccuggucuugacauccacggaaguu
uucagagaugagaaugugccuucgggaaccgugagacaggugcugcaugg
cugucgucagcucguguugugaaauguuggguuaagucccgcaacgagcg
caacccuuauccuuuguugccagcgguccggccgggaacucaaaggagac
ugccagugauaaacuggaggaagguggggaugacgucaagucaucauggc
ccuuacgaccagggcuacacacgugcuacaauggcgcauacaaagagaag
cgaccucgcgagagcaagcggaccucauaaagugcgucguaguccggauu
ggagucugcaacucgacuccaugaagucggaaucgcuaguaaucguggau
cagaaugccacggugaauacguucccgggccuuguacacaccgcccguca
caccaugggaguggguugcaaaagaaguagguagcuuaaccuucgggagg
gcgcuuaccacuuugugauucaugacuggggugaagucguaacaagguaa
ccguaggggaaccugcgguuggauca

........<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<..<.
.<<<.<<<..<<<..<<.<...<<<<<<<..>>>>.>>>.>..>>>>>..
....<<.......<<<<<<<..<<...<<<<<<<.<.<<.....<<<<<.
.....>>>>>......>>.>.....<<<....>>>....<<<<<<..<<.
...>>>>>>>>.>>>>>>>..>>.>>>>>>><<<....<<<..<<<<<<<
.........>>>>>>>>>>......>>>..<<<<<<<<....>>>>...>
>>>.>>.<<<<<.<.........>>>>>>.<<<<....>>>>...>>>>>
>>........<<<...<<<<<....>>>>.>>>>..>>.>>>>>>..<<<
<......<<<<....>>>>.....>>>>...<.<<<<<......<.<<<<
<<<<.......>>>>>>>>.>........>>>>>....>..<<<<<(((.
..<<<<<.....<<.)))>>.......>>>>>>>>>>..>>>>>>>>>..
........<<<((.....<<<<...<<<.<<<<<<<.<<<<<<<<<<...
...<<<<<<.....>>>>>>....>>>>>>>>..>>>>>>>>>...<<<<
<<<<...<<<<<<<....<<<<<<<<...<<<......>>>......>>>
>>>>>...........<<....>>.>>>>>>>..>>>.>>>>>...>>>.
..>>>>....<<<<<<...<<...<<<<.........>>>>...>>>>>>
>>..........<<<<<<.<<<<<<<<<<<<<.....>>>>>>>>>>>>>
..<<..))>>.....>>>>>>.>>>.<<<......<<<<....>>>>...
.>>>..)))).]<<<<<.<<<<<<<.<<.<<<<<<..<<<<<<<<<<...
...<<........>>..........<<<<<<<......<<<<<<<..<<<
<<<<....>>>>>>>...<<....>>..>>>>>.>>.<<<.<<<..<<<<
<<.......<<<<<<<<<....>>>..<<<<......>>>>..>>>>>>.
....<<<..<<<<<<<...<<..<<<.....>>>>>....>>>>>>>...
..<<<<<.....>>>>>.........>>>.........>>>...>>>>>>
>>>...>>>>>>>...>>.>>>>>>>>.....<<<<<<<.....<<<..<
<...<<<....>>>...>>....>>>.....>>>>>>>......<....<
<<<<<<........>>>>>>>....>.....>>>>>>....<<<<<<<..
.......>>>>>>>......>>...>>>>>>>>>>.>>....<..<<.<.
<<<<.<<<..<<<<<<<<<<<<....<<<<<<.<<<<..<<....>>.>>
>>>>>>>>...>>>>>>>>>>>>..>>>.>>>>..>.>>...>.....<<
<<<<<<<....>>>>>>>>>......

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7n30 Structural basis of early translocation events on the ribosome.
• Resolution: 2.66 Å
• Binding residue (original residue number in PDB): V21 S22 K23 T24 V25 K26 G27 R29 Y50 K52 R54 K62 R69 H89 T90 F95 Q97 A99 S100 T103 A107 G108 R112 L124 A125 K126 A127 Y128 S130 T131 N132 N135 R138
• Binding residue (residue number reindexed from 1): V11 S12 K13 T14 V15 K16 G17 R19 Y40 K42 R44 K52 R59 H79 T80 F85 Q87 A89 S90 T93 A97 G98 R102 L114 A115 K116 A117 Y118 S120 T121 N122 N125 R128

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0046677 response to antibiotic
• GO:1990145 maintenance of translational fidelity

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0015935 small ribosomal subunit
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7n30, PDBe:7n30, PDBj:7n30
• PDBsum: 7n30
• PubMed: 34234344
• UniProt: P0A7W1|RS5_ECOLI Small ribosomal subunit protein uS5 (Gene Name=rpsE)