Structure of PDB 7wtn Chain SB Binding Site BS01

Receptor Information

>7wtn Chain SB (length=216) Species: 4932 (Saccharomyces cerevisiae)

VVDPFTRKEWFDIKAPSTFENRNVGKTLVNKSTGLKSASDALKGRVVEVC
LADLQGSEDHSFRKIKLRVDEVQGKNLLTNFHGMDFTTDKLRSMVRKWQT
LIEANVTVKTSDDYVLRIFAIAFTRKQANQVKRHSYAQSSHIRAIRKVIS
EILTKEVQGSTLAQLTSKLIPEVINKEIENATKDIFPLQNIHVRKVKLLK
QPKFDVGALMALHGEG

Ligand information

>7wtn Chain C2 (length=1200)

uaucugguugauccugccaguagucauaugcuugucucaaagauuaagcc
augcaugucuaaguauaagcaaauacagugaaacugcgaauggcucauua
aaucaguuaucguuuauuugauaguuccucuacaugguauaacuguggua
auucuagagcuaauacaugcuuaaaaucucgacccuuuggaagagaugua
uuuauuagauaaaaaaucaaugucuucggacucuuugaugauucauaaua
acuuuucgaaucgcauggccuugugcuggcgaugguucauucaaauuucu
gcccuaucaacuuucgaugguaggauaguggccuaccaugguuucaacgg
guaacggggaauaaggguucgauuccggagagggagccugagaaacggcu
accacauccaaggaaggcagcaggcgcgcaaauuacccaauccuaauuca
gggagguagugacaauaaauaacgauacagggucuuguaauuggaaugag
uacaauguaaauaccuuaacgaggaacaauuggagggcaagucuggugcc
agcagccgcgguaauuccagcuccaauagcguauauuaaaguuguugcag
uuaaaaagcucguaguugaacuuugggcccgguuaacggggccuuuccuu
cuggcuaaaccaggacuuuuacuuugaaaaaauuagaguguucaaagcag
gcguauugcucgaauauauuagcauggaauaauagaauaggacguuuggu
ucuauuuuguugguuucuaggaccaucguaaugauuaauagggacggucg
ggggcaucaguauucaauugucagaggugaaauucuuggauuuauugaag
acuaacuacugcgaaagcauuugccaaggacguuuucauuaaucaagaac
gaaaguuaggggaucgaagaugaucagauaccgucguagucuuaaccaua
aacuaugccgacuagggaucgggugguguuuuuuuaaugacccacucggc
accuuacgagaaaucaaagucuuuggguucuggggggaguauggucgcaa
ggcugaaacuuaaaggaauugaccgcgcuaguaccgauugaauggcuuag
ugaggccucaggaucugcagagcggagaauuuggacaaacuuggucauuu
agaggaacuaaaaguagguuuccguaggugaaccugcggaaggaucauua

...<<<<<...[[[[>>>>>[[[[.((((((................[[[
.[[[..<<....<<....<<.......>>...>>.>>......{{.....
...[[[..{{..{{....[[[...............<<....<<.<<<..
...>>>.>>.....>>.........<<<<<..<<....>>..>>>>>[..
.((((((......<<<<<<<<<<<....>>>....<<......>>)))))
).....]...<<<<..<<<.....>>>.>>>>....]]]...}}}}..]]
].<<<....<<<....<<<<<<<<.......>>>>>>>>>>>......>>
>...<<<.<<<<....>>>>....>>>.}}.<<.<<<..........>>>
.>>.<.<<<..>>>.>...]]]]]].........<<<....<<<.....>
>>..>>>...............<<<<<<<<....>>>>>.>>>......<
<..<...........>..>>.........<<<<<........<<<<.<<<
.<<....>>>>>....>>>>.>>>>>..))))))]]]].........[[[
{{.......{{...[[[.{{....<<<<<<.<<..>>.>>>>>>....<<
<<<<.....>>>>>>...<<<<<.<<.......<<...<.......>..<
<<.....>>>....>>......>>.>>..>>>.........[[[..((((
(((.....>>>>>>>>..))))))).]]].....}}....<<<<<<.<<.
..<<<<..<<..<<<<<<.<...<<<......>>>......>.>>>>>>.
.>>.......<<....>>...>>>>...>>>>>.>>>...]]]...}}..
....<<<<<<<...<...<<<<.........>>>>...>>>>>>>>....
......<<<.<<.<<<..<<<<<<<<.<<<........>>>>>>>>>>>.
.>>>...<<..}}>>...>>.....>>>.]]].<<<......<<<<....
>>>>....>>>..]]]]....<<<....<<<<.<<....<<<<<<<<.<<
<..<.<<..<...<<<<.....>>>>...>..>>.>..>>>.>>>>>>>>
...>>.>>>.>.....>>><<<<<<<<<....>>>>>>>>>.........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7wtn The nucleoplasmic phase of pre-40S formation prior to nuclear export.
• Resolution: 3.4 Å
• Binding residue (original residue number in PDB): V114 K116 W117 Q118 L120 E122 N124 R136 F138 Q146 N148 Q149 K151 H153 Y155 Q157 S158 S159 R165 I204 F205 K216
• Binding residue (residue number reindexed from 1): V95 K97 W98 Q99 L101 E103 N105 R117 F119 Q127 N129 Q130 K132 H134 Y136 Q138 S139 S140 R146 I185 F186 K197

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:0030686 90S preribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7wtn, PDBe:7wtn, PDBj:7wtn
• PDBsum: 7wtn
• PubMed: 36321656
• UniProt: P33442|RS3A1_YEAST Small ribosomal subunit protein eS1A (Gene Name=RPS1A)