Structure of PDB 8fn2 Chain S Binding Site BS01

Receptor Information

>8fn2 Chain S (length=114) Species: 224326 (Borreliella burgdorferi B31)

ARAKNGTVHVARRKRILKKTKGFWGTKKSNYKKAKDTLRKGMMYATRDRK
VRKRDFRRLWISRISAALSDTGVTYSRFIEGLLKSNIKINRKILSNLAIE
DVEAFKKIVLEIRR

Ligand information

>8fn2 Chain A (length=2762)

uggucaaaguaauaagagucuauggugaaugccuaggagcuuuaaggcga
agaaggucgugguaagcugcgaaaagcuugggggagaagcaaacauuuau
ugaucccaagauuaccgaauggaguaauccagcuagcaagaugcuagcua
ucuauuauuuaaauaauagaggcgauaccaggggaagugaaccaucuaag
uacccugaggaaaagaaaucaaagagauucccuuaguaguggcgagcgaa
aagggaguagcccaaacuuuaaaugugucaagcugcagagcguugcauuu
augggguuguaggacguuuaggcuuagucuguaauaagcaaaaaaguuac
aaaauauuuauauagaagaauaaucuggaaaguuuaaccaaagaagguga
uaguccuguaauuuaaauguaaauaucuuuuuaaaauguuccugaguagg
acgaggcacgagaaaccuuguuugaagcuggggagaccacucuccaaggc
uaaauacuagaaagcuaccgauagagaagaguaccgugagggaaagguga
aaagaaccccgggaggggagugaaauagaacugaaaccguagacuuacaa
gcagucaaagccguaauuuauugcggugauggcgugccuuuugcauaaug
aaccugcgaguuaucaugucuagcaagauuaaagcauagaagugcuggag
ucgaagcgaaagcgagucuuaaaagggcgauuuaguuagaugugguagac
ccgaagccgagugaucuauuuauggccaggcugaagcuuggguaaaacca
aguggagggccgaacucuagucuguuuaaaaaggcagggaugagcuguga
auaggagugaaaggcuaaacaaacucggagauagcugguucuccccgaaa
uggauuuaaguucagccuuauuuuaguuuaauagagguagagcacuaauu
gagcuagggccugucaaaggguaccaaacucaguuaaacuccgaaugcua
uuaaaugaugaauaggagugagacuaugggcgauaagguucauagucgag
agggaaacaacccagaccaacagcuaaggucucaaaaauguguuaagugg
aaaaggagguuuagguacguaaacagccaggagguuggcuuagaagcagc
cauaccuuuaaagagugcguaauagcucacuggucgaguacuuaagcgcc
gauaauguaacggggcuaaacacauuaccgaagcuuuggaucuuaacgaa
aguuaagaugguaggggagcguucuguaagccagagaaguuaagcuggaa
aguuugauggagguaucagaagugagaaugcagguaugaguaacgaaaaa
augggugagauucccauucgccgaaaaccuaagguuuccuggguaaaggu
cgucuucccaggguuagucggccccuaaggcaaagcugaaaaguguaguc
gaugggaaacggguuaauauucccguaccucuuauaguuucgauggagug
acgcaugagguuaacuacugcuaggcgaugguuguccuaguuuaagcauu
aaggcgaugaucuuaauaggaaaauccguuaagagagcuaagaugugaug
augagugcuauuuagguagcaugaaauguagguagucaaggugccaagaa
auagcuucuaagguuaggcuauaagggaccguaccgcaaaccgacacagg
uaggugggaugaaaauucuaaggcgcgcgagagaauccacguuaaggaac
ucugcaaaauacguacguaacuucgggauaaguacgaccuaagcaauuag
guagcauaaaaaugguccaaacgacuguuuaccaaaaacacaggucucug
caaaucuguaaagagaaguauagggacugacaccugcccggugcuaguaa
acggcuaagguagccggguaaguuccgacgaaugguguaacgauuuggac
gcugucucaacguggagcucggugaaauugaaguaucggugaagaugccg
auuacuugugguuagacggaaagaccccgugaaccuuuacuauagcuugg
uauugagauuugauuaauuaauuagguuucuaacuuauagaaauaugagg
agagugccagguggguaguuugacuggggcggucgccuccuaaagaguaa
cggaggugcgcaaagguuaccuuagagugguuggaaaucacucuguaagu
guaaaggcauaagguagcuuaacuguaagacugacaagucgaacagauac
gaaaguaggucuuagugaucuggcgguggcaaguggaagcgccgucacuu
aacgaauaaaagguacuccggggauaacaggcuuauccuucccaagaguu
cacaucgacggaaggguuuggcaccucgaugucggcucaucgcauccuag
ggcuggagcagguccuaaggguauggcuguucgccauuuaaagcgguacg
cgagcuggguucagaacgucgugagacaguuuggucccuaucugccacaa
gcguuggauauuugagaggagcuaucuuuaguacgagaggaccgagaugg
acgaaccucuagugugccaguuauccugccaaggguaagugcuggguagc
uacguucggaaaggauaaccgcugaaagcaucuaagugggaagccuuccu
caagaugagauauccuuuaaggguccuggaagaauaccagguugauaggu
uagaaguguaaguauagcaauauauuaagcugacuaauacuaauuacccg
uaucuuuggcca

.<<<<<<<<......<<<<<<<<<<<.....<<..<<<<<<<<.<<<<..
....<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>..
...>>>>>>>...>>>....<<<....>>><<<<<<<.....>>>>>>>.
<<<<<<<.<..>.>>>>>>>.>>....<<<<<<.....<<....>>....
..>>>>>.>.........<<...>>..<<<<<<.....<<.....>>...
.>>>>>>..<<<....<<.<<<<<<<.......<<.<<<.....>>>>>>
>.>>......[<<<<.[....<<<<<.>>>.>>.>>>>>.<<<<......
..<.<<<<.<<<<.<<....<<<.<......>.>>>((......<<<...
..))>>>....>>...>>>>>>.>>>.>>>>...]..>>>>.].....<<
<<<<<<.........>>>>>>>>......<<<<<..((((>>>>>..>>>
......>>..>>>>>>.>>..............<<....>>....>>...
......<<<<....>>>>.................>>>>>>>>>>>....
...<<<<..<<<<<<<....>>>>>>>..>>>>...<.<........>.>
.<<<<<<....<<<<<<<<<<<<<..<<<...<<<<<....>>>>>...>
>>...<<....>>..<<<<.....>>>>......>>>>>>>>>>>>>..<
<.<..<<<<<<<.....<<<<<<<<<..<<<....<<<<<<......>>>
>>>.....>>>...<<<<.<<<<........>>>>>>>>....>>>>>>>
>>...<<<.....>>>......>>>>>>...>..>.>>..<<<<<<....
<<<<<....>>>>><<<<<<<<<..<<<<<<<<<.....<<<...<<<<<
<<<...<<<<<<......>>>>.>>...>>>>>>>>..>>.>.....>>>
>>>>>>...>>>>>>>.>>..<<<<<<<<<<......>>>>>>>>>>...
.<<<......>>>...<<<<.<<<...<<<((....<<<<<<<.......
....<<..<<<<<<<<<........<<<<<.<<...<<<.........>>
>....>>.........<<......>>...>>>>>....>>>>>>>>>.>>
............)).....>>>>>>>>>>...>>>>>>><<<<<<<<...
.>>>>>>>>....>>>>>>..<<<<<...<<<......<<<<<<<<....
>>>>>>>>...>>>..>>>>>........>>>>>><......<<.....<
<<<<<.......>>>>>><<<<....<<<<<.<<<..<<<<<<...<<..
...>>.>>>>>>....((<<<.<<<...<<<...<<<....>>>...>>>
...>>>..<<<<<........>>>>>)).<<<<<<<<<.<<...<<<<<.
..<<<<..<<<...<<<<.<<<<<<...[[[[...>>>>>><<..<<<<<
..<<<........<<<............>>>.....>>>..>>>>>...>
>...<<<<<<<....>>>>>>>.....>>>>...>>>...>>>>......
...>>>>>...>>....>>>>>>>>>..>>>.>>>.....<<......>>
>>>>>.<<<.......>>>..>>>>..<<<<....<<<<<<<<.<<....
.....<.....<<<<<...............>>>>><<<<<<....>>>>
>>............<<<<<<<<<..<<<.........>>>..<<<<<<<.
....<<<....>>>.......>>>>>>>.<<<<<..<<..<<<<..>>..
>>>>........<.<<<.......>>>.>....>>>>>..>>.>>>>>>>
..>..>>.>>>>>>>>.>>>>>>.....>.....<<<<<........>>>
>>..<<<<<<<<.<<<.<<......<<<<.<<<<<<..<<<<<.<<<<<<
<<<.<<<<<<<<<<<<..>>>>>>>>>>>>...<<<<<<....>>>>>>.
...>>>>>>>>>..>>>>>..<<<<.....>>>><<<<<<..........
.>>>>>>..<<...<<<<<<<.<<<<<<<<.......>>>>>>>>...<<
.[.(((>>....>>>>>>>...<<<<..<<<<....>>>>..>>>>.<<<
....>>>.>>)))]......<<<<<<<.<<..))))..>>>>>>>>>...
..........>>>>..>>>>>>.......<<<<.<.<<<<<<...<<...
....>>...>>>>>>...>>>>.>.......<<<<<.<<<<<<..<<<<<
<<<<......>>>>>>..>>>.<<<<<.....>>>>>.....>>>>>..>
...>>>.>>....<<<<<<<....>>>.>>>>....>>..>>>>>>>>>>
>....<<<<<<<...<<<<..<<<<<<<...............>>>>>>>
.<<<<<...<<<<...<<<<<<<<<<<]]]]>>>>>>...>>>>>...>>
>>.>>>>>..<<<<....<<<<<...........>>>>>....>>>>>>>
>.......>>>>>>>.....<<<<<<<<<.......>>>>>....<<<<<
<<<.<<<....<<<<<....>>>>>...>>>..>>>>>.>>>...>>>>.
...>>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8fn2 The structure of a hibernating ribosome in a Lyme disease pathogen.
• Resolution: 3.4 Å
• Binding residue (original residue number in PDB): A2 R3 K5 N6 H10 R13 R14 K15 R16 K22 F24 W25 G26 K29 Y32 K33 K34 K36 D37 T38 K41 Y45 R48 D49 R50 K51 R53 K54 R55 F57 R58 R59 W61 I62 Y76 S77 R78 E81 N91 R92 K93
• Binding residue (residue number reindexed from 1): A1 R2 K4 N5 H9 R12 R13 K14 R15 K21 F23 W24 G25 K28 Y31 K32 K33 K35 D36 T37 K40 Y44 R47 D48 R49 K50 R52 K53 R54 F56 R57 R58 W60 I61 Y75 S76 R77 E80 N90 R91 K92

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8fn2, PDBe:8fn2, PDBj:8fn2
• PDBsum: 8fn2
• PubMed: 37907464
• UniProt: O51206|RL20_BORBU Large ribosomal subunit protein bL20 (Gene Name=rplT)