Structure of PDB 7sae Chain S Binding Site BS01

Receptor Information

>7sae Chain S (length=109) Species: 1423 (Bacillus subtilis)

QAKAVARTVRIAPRKARLVMDLIRGKQVGEAVSILNLTPRAASPIIEKVL
KSAIANAEHNYEMDANNLVISQAFVDEGPTLKRFRPRAMGRASQINKRTS
HITIVVSEK

Ligand information

>7sae Chain A (length=2434)

uuaaguuagaaagggcgcacgguggaugccuuggcacuaggagccgauga
aggacgggacgaacaccgauaugcuucggggagcuguaagcaagcuuuga
uccggagauuuccgaauggggaaacccaccacucguaauggagugguauc
cauaucugaauucauaggauaugagaaggcagacccggggaacugaaaca
ucuaaguacccggaggaagagaaagcaaaugcgauucccugaguagcggc
gagcgaaacgggaucagcccaaaccaagaggcuugccucuugggguugua
ggacacucuguacggaguuacaaaggaacgagguagaugaagaggucugg
aaaggcccgccauaggagguaacagcccuguagucaaaacuucguucucu
ccugaguggauccugaguacggcggaacacgugaaauuccgucggaaucc
gggaggaccaucucccaaggcuaaauacucccuagugaccgauagugaac
caguaccgugagggaaaggugaaaagcaccccggaaggggagugaaagag
auccugaaaccgugugccuacaaguagucagagcccguuaacgggugaug
gcgugccuuuuguagaaugaaccggcgaguuacgaucucgugcaagguua
agcagaagaugcggagccgcagcgaaagcgagucugaauagggcgcauga
guacguggucguagacccgaaaccaggugaucuacccauguccaggguga
aguucagguaacacugaauggaggcccgaacccacgcacguugaaaagug
cggggaugagguguggguaggggugaaaugccaaucgaaccuggagauag
cugguucucuccgaaauagcuuuagggcuagccucaagguaagagucuug
gagguagagcacugauuggacuaggaauucagucaaacuccgaaugccaa
ugacuuauccuugggagucagacugcgagugauaagauccguagucgaaa
gggaaacagcccagaccgccagcuaaggucccaaaguauacguuaagugg
aaaaggauguggaguugacucugcgccgaaaauguaccggggcuaaacgu
aucaccgaagcugcggacuguucuucgaacagugguaggagagcguucua
agggcugugaagccagaccggaaggacugguggagcgcuuagaagugaga
augccgguaugaguagcgaaagaggggugagaauccccuccaccgaaugc
cuaagguuuccugaggaaggcucguccgcucaggguuagucgggaccuaa
gccgaggccgaaaggcguaggcgauggacaacagguugauauuccuguac
caccuccucaccauuugagcaauggggggacgcaggaggauaggguaagc
gcgguauuggauauccgcguccaagcaguuaggcugggaaauaggcaaau
ccguuucccauaagccugagcugugauggcgagcgaaauauaguagcgaa
guuccugauuccacacugccaagaaaagccucuagcgaggugagaggugc
ccguaccgcaaaccgacacagguaggcgaggagagaauccuaaggugauc
gagagaacucucguuaaggaacucggcaaaaugaccccguaacuucggga
gaaggggugcucuguuagggugcaagcccgagagagccgcagugaauagg
cccaggcgacuguuuagcaaaaacacaggucucugcgaagccguaaggcg
aaguauaggggcugacgccuaggcgcaacgaucugggcacugucucaacg
agagacucggugaaauuauaguaccugugaagaugcagguuacccgcgac
aggacggaaagaccccguggagcuuuacugcagccugauauugaaugucc
uucuaacccgccgcccuuaucgggcggggagacagugucaggugggcagu
uugacuggggcggucgccuccuaaaagguaacggaggcgcccaaagguuc
ccucagaaugguuggaaaucauucgcagaguguaaaggcacaagggagcu
ugacugcgagaccuacaagucgagcagggacgaaagucgggcuuagugau
ccggugguuccgcauggaagggccaucgcucaacggauaaaagcuacccc
gguccgucgcgggcgcaggaaauuugagaggagcuguccuuaguacgaga
ggaccgggauggacgcaccgcugguguaccaguuguucugccaagggcau
cgcuggguagcuaugugcggacgggauaagugcugaaagcaucuaagcau
gaagccccccucaagaugagauuucccauuccgcaaggaaguaagauccc
ugaaagaugaucagguugauaggucugagguggaaguguggcgacacaug
gagcugacagauacuaaucgaucgaggacuuaac

<<<<<<......<<<<<<<<<<<....<<<..<<<<<<<<<<<<<.....
.<<<<<<.....((.>>>.....<<<<<<<..<<<)).....>>>.....
>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<
<<<<<<<.........>>>>>>>.>>.>>....<<<<<<.....<.....
.>......>>>>>.>.........<<....>>...<<<<......<<...
..>>.....>>>>...<<<....<<<<<<<<<..>>>>>>>>>......<
<<<<<<<<.....<<<<.......<<<<<<<<<<.<<<.....<<<<...
...>>>>.((......<<<.....))>>>...>>>...>>>>>>>>>>>>
>>.>>>>>..>>>>.....<<<<<<<<.........>>>>>>>>......
<<<<<..((((>>>>>..>>>......>>.>>>>>>>.>>..........
.....<<....>>....>>.........<<<<....>>>>..........
...>....>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>
>>...<.<........>.>.<<<<<<....<<<<<<<.<<<<<..<<<..
.<<<.....>>>...>>>...<<....>>..<<<<.....>>>>......
>>>>>.>>>>>>>..<<.....<<<<<<.....<<<<<<<<<..<<<...
.<<<<<<......>>>>>>.....>>>...<<<.<<<<<........>>>
>>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>......
..>>..<<<<<<....<<<<<....>>>>><<<<<<<<<...<<<<<<<<
<.....<<<...<<<...<.........>...>>>..>>.>.....>>>>
.>>>>>..>>>>>>>.>>..<<<<<<<.<<......>>.>>>>>>>....
<<<......>>>...<<<<.<<<...<<<(((.....<<<<<<.......
....<<...<<<<<<..>>>>>>..>>...........))).....>>>>
>>.>>>...>>>>>>><<<<<<<...>>>>>>>....>>>>>><.<<<<<
<<.<<......<<<<<.<<....>>.>>>>>...>>.>>>>>>>..>...
..>>>>>><......<<....<<<<<<.......>>>>>><<<<....<<
<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<.<<...
<<<...<<<....>>>...>>>...>>...<<<<<........>>>>>))
<<<<<.<<<<<<......<<...<<<<....<<<<..<<<..<<<.<.<<
<.<...[.[....>.>>>.<<..<....<.<<<<.<<<<<<<.<<.....
>>>>>>>>>....>>>>..>...>...>><..<<...........>>...
>>.>>>...>>>...>>>>.........>>>>..>>..>>>>>>>>>>>>
>>>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>..<
<<<....<<<<<<<<.<<<..............<<<<<....<<..>>..
...>>>>><<<<<....<<<<....>>>>...>>>>>............<
<<<<<<<<..<<<.........>>>..<<<<<<<.....<<<....>>>.
......>>>>>>>..<<<<..>>>>...>>.>>>>>>>....>>>.>>>>
>>>>.>>>>>>.....>.....<<<<<........>>>>>..<<<<<<<<
.<<................<<<<<..<<<<..<<<<<<<<<.<<<.....
>>>....<<.<<<<<<.....>>>>>>>>.....>>>>>>>>>...>>>>
..<<<.......>>><<<<<<...........>>>>>>..<<...<<<<<
.<<...<<<.<.......>.>>>......<<...(((>>....>>>>>>>
...<<<<..<<<......>>>..>>>>.<<<....>>>.>>)))......
.<<<<<<<.<<..))))..>>>>>>>>>.............>>>>...>.
...>>>>>>>>>>....<<<<<<<...<<<<..<<<<<<<..........
.....>>>>>>>.<<<<<...<<<<...<<<<<<<<<<<.].]>>>>>>.
.>>>>>...>>>>.>>>>>...<<<....<<<<<...........>>>>>
....>>>.>>>>.......>>>>>>>..<<<<....>>>>....<<<<<<
<<<.......>>>>>....<<<<<<<<.<<<....<<<<<....>>>>>.
..>>>..>>>>>.>>>...>>>>....>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7sae RbgA ensures the correct timing in the maturation of the 50S subunits functional sites.
• Resolution: 3.0 Å
• Binding residue (original residue number in PDB): A7 R8 R11 R15 K16 P40 R41 A42 A56 N57 N61 Y62 D77 E78 R86 P87 R88 M90 G91 A93 S94
• Binding residue (residue number reindexed from 1): A6 R7 R10 R14 K15 P39 R40 A41 A55 N56 N60 Y61 D76 E77 R85 P86 R87 M89 G90 A92 S93

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7sae, PDBe:7sae, PDBj:7sae
• PDBsum: 7sae
• PubMed: 35141754
• UniProt: P42060|RL22_BACSU Large ribosomal subunit protein uL22 (Gene Name=rplV)