Structure of PDB 7nhl Chain S Binding Site BS01

Receptor Information

>7nhl Chain S (length=114) Species: 93061 (Staphylococcus aureus subsp. aureus NCTC 8325)

TNHKLIEAVTKSQLRTDLPSFRPGDTLRVHVRIIEGTRERIQVFEGVVIK
RRGGGVSETFTVRKISSGVGVERTFPLHTPKIEKIEVKRRGKVRRAKLYY
LRSLRGKAARIQEI

Ligand information

>7nhl Chain A (length=2914)

uuaaguuauuaagggcgcacgguggaugccuuggcacuagaagccgauga
aggacguuacuaacgacgauaugcuuuggggagcuguaaguaagcuuuga
uccagagauuuccgaauggggaaacccagcaugaguuaugucauguuauc
gauaugugaauacauagcauaucagaaggcacacccggagaacugaaaca
ucuuaguacccggaggaagagaaagaaaauucgauucccuuaguagcggc
gagcgaaaugggaagagcccaaaccaacaagcuugcuuguugggguugua
ggacacucuauacggaguuacaaaggacgacauuagacgaaucaucugga
aagaugaaucaaagaagguaauaauccuguagucgaaaauguugucucuc
uugaguggauccugaguacgacggagcacgugaaauuccgucggaaucug
ggaggaccaucuccuaaggcuaaauacucucuagugaccgauagugaacc
aguaccgugagggaaaggugaaaagcaccccggaaggggagugaaauaga
accugaaaccgugugcuuacaaguagucagagcccguuaaugggugaugg
cgugccuuuuguagaaugaaccggcgaguuacgauuugaugcaagguuaa
gcaguaaauguggagccguagcgaaagcgagucugaauagggcguuuagu
auuuggucguagacccgaaaccaggugaucuacccuuggucagguugaag
uucagguaacacugaauggaggaccgaaccgacuuacguugaaaagugag
cggaugaacugaggguagcggagaaauuccaaucgaaccuggagauagcu
gguucucuccgaaauagcuuuagggcuagccucaagugaugauuauugga
gguagagcacuguuuggacgaggggccccggguuaccgaauucagacaaa
cuccgaaugccaauuaauuuaacuugggagucagaacaugggugauaagg
uccguguucgaaagggaaacagcccagaccaccagcuaaggucccaaaau
auauguuaaguggaaaaggauguggcguugcccagacaacuaggauguug
gcuuagaagcagccaucauuuaaagagugcguaauagcucacuagucgag
ugacacugcgccgaaaauguaccggggcuaaacauauuaccgaagcugug
gauuguccuuuggacaaugguaggagagcguucuaagggcguugaagcau
gaucguaaggacauguggagcgcuuagaagugagaaugccggugugaguc
gcgaaagacgggugagaaucccguccaccgauugacuaagguuuccagag
gaaggcucguccgcucuggguuagucggguccuaagcugaggccgacagg
cguaggcgauggauaacagguugauauuccuguaccaccuauaaucguuu
uaaucgauggggggacgcaguaggauaggcgaagcgugcgauuggauugc
acgucuaagcaguaaggcugaguauuaggcaaauccgguacucguuaagg
cugagcugugauggggagaagacauugagucuucgagucguugauuucac
acugccgagaaaagccucuagauagaaaauaggugcccguaccgcaaacc
gacacagguagucaagaugagaauucuaaggugagcgagcgaacucucgu
uaaggaacucggcaaaaugaccccguaacuucgggagaaggggugcucuu
uaggguuaacgcccagaagagccgcagugaauaggcccaagcgacuguuu
aucaaaaacacaggucucugcuaaaccguaaggugauguauaggggcuga
cgccugcccggugcuggaagguuaagaggagugguuagcuucugcgaagc
uacgaaucgaagccccaguaaacggcggccguaacuauaacgguccuaag
guagcgaaauuccuugucggguaaguuccgacccgcacgaaaggcguaac
gauuugggcacugucucaacgagagacucggugaaaucauaguaccugug
aagaugcagguuacccgcgacaggacggaaagaccccguggagcuuuacu
guagccugauauugaaauucggcacagcuuguacaggauagguaggagcc
uuugaaacgugagcgcuagcuuacguggaggcgcuggugggauacuaccc
uagcuguguuggcuuucuaacccgcaccacuuaucguggugggagacagu
gucaggcgggcaguuugacuggggcggucgccuccuaaaagguaacggag
gcgcucaaagguucccucagaaugguuggaaaucauucauagaguguaaa
ggcauaagggagcuugacugcgagaccuacaagucgagcagggucgaaag
acggacuuagugauccggugguuccgcauggaagggccaucgcucaacgg
auaaaagcuaccccggggauaacaggcuuaucucccccaagaguucacau
cgacggggagguuuggcaccucgaugucggcucaucgcauccuggggcug
uagucggucccaaggguugggcuguucgcccauuaaagcgguacgcgagc
uggguucagaacgucgugagacaguucggucccuauccgucgugggcgua
ggaaauuugagaggagcuguccuuaguacgagaggaccgggauggacaua
ccucugguguaccaguugucgugccaacggcauagcuggguagcuaugug
uggacgggauaagugcugaaagcaucuaagcaugaagccccccucaagau
gagauuucccaacuucgguuauaagaucccucaaagaugaugagguuaau
agguucgagguggaagcauggugacauguggagcugacgaauacuaaucg
aucgaagacuuaau

<<<<<<......<<<<<<<<<<<.....[[.(((<<<<<<<[[<<.....
.<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>.....
>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<
<<<<<<<.........>>>>>>>.>>.>>....<<<<<......<<....
>>.......>>>>.>.........<<....>>..<<<<<......<<...
..>>.....>>>>>..(((....<<<<<<<<....>>>>>>>>......<
<<<<<<<<.....<<<<.......<<<<<<<<<<.<<<...<<<<<....
..>>>>>((......<<<.....))>>>...>>>...>>>>>>>>>>>>>
>.>>>>>..>>>>.....<<<<<<<<.........>>>>>>>>......<
<<<<..<<<<>>>>>..)))......]].>>>>>>>.)))..........
....<<....>>....]].........<<<<....>>>>...........
.......>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>>
>...<.<........>.>.<<<<<<....<<<<<<<.<<<<<..<<<...
<<<.....>>>...>>>...<<....>>..<<<<.....>>>>.....>>
>>>.>>>>>>>..<<.<...<<<<<<.....<<<<<<<<<..<<<....<
<<<<<......>>>>>>.....>>>...<<<.<<<<<........>>>>>
>>>....>>>>>>>>>...<<<.....>>>......>>>>>>......>.
>>..<<<<<<....<<<<<....>>>>><<<<<<<<<...<<<<<<<<<.
....<<<...<<<<<<<<....<.<<<<.>>>>..>....>>>>>>>>..
>>.>.....>>>>.>>>>>..>>>>>>>.>>..<<<<<<<<<<......>
>>>>>>>>>....<<<......>>>...<<<<.<<<...<<<(((.....
<<<<<<...........<<..<<<<.<<<<........<<<<<.<<...<
<<<.......>>>>...>>.........<<......>>...>>>>>....
>>>>.>>>>.>>...........))).....>>>>>>.>>>...>>>>>>
><<<<<<<...>>>>>>>....>>>>>><.<<<<<<<.<<......<<<<
<.<<....>>.>>>>>...>>.>>>>>>>..>.....>>>>>><......
<<....<<<<<<.......>>>>>><<<<....<<<<<.<<...<<<<<<
...<<.....>>.>>>>>>....((<<<<.<<...<<<...<<<....>>
>...>>>...>>...<<<<<........>>>>>))<<<<<<<..<<....
..<<<...<<<<....<<<<..<<<..<<<<<.<<<<<<...(((...>>
>>>><<..<<<<<..<<<<<<<<<<..<<.....>>.>>>>>>.....>>
>>..>>>>>...>>...<<<<<<.....>>>>>>...>>>>>...>>>..
.>>>>.........>>>>..>>>.>>..>>>>>>>>>>>..>>.....<<
......>>>>>>>.<<<.......>>>..>>>>..<<<<....<<<<<<<
<.<<.........<.....<<<<<...............>>>>><<<<<<
<.<<<<....>>>>.>>>>>>>............<<<<<<<<<..<<<..
.......>>>..<<<<<<<.....<<<....>>>.......>>>>>>>.<
<<<<.<<<..<<<<<<<..<<<...<<...<<<<<..<<....>>...>>
>>>...>>...>>>>>>>>..>>>>><<<<<<.......>>>>>>.....
...<...........<<<<<<.....>>>>>>.....>....>>>>>..>
>.>>>>>>>..>..>>.>>>>>>>>.>>>>>>.....>.....<<<<<..
......>>>>>..<<<<<<<<.(((.[[......<<<<.[[<<<<..<<<
<<.<<<<<<<<<.<<<<.<<<<<<<<<<<...........<<<<<..<<<
......<<<<<<<<....>>>>>>>>...>>>............>>>>>.
.>>>>>>>>>>>.>>>>....<<.<<<<<<.....>>>>>>>>.....>>
>>>>>>>..>>>>>..<<<<.....>>>><<<<<<...........>>>>
>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....<<.[.((
(>>....>>>>>>>...<<<<..<<<......>>>..>>>>.<<<....>
>>.>>)))]......<<<<<<<.((..>>>>..))>>>>>>>........
.....>>>>..]]>>>>.......<<<<...<<<<<<...<<.......>
>...>>>>>>....>>>.>.......<<<<<.<<<<<<..<<<<<<<<<.
.....>>>>>>..>>>.<<<<<.....>>>>>.....>>>>>..>...>>
>.>>....<<<<<<<....>>>.>>>>....]]..)))>>>>>>>>....
<<<<<<<...<<<<..<<<<<<<...............>>>>>>>.<<<<
<...<<<<...<<<<<<<<<<<.)))>>>>>>..>>>>>...>>>>.>>>
>>...<<<....<<<<<...........>>>>>....>>>.>>>>.....
..>>>>>>>.<<<....>>>....<<<<<<<<<.......>>>>>....<
<<<<<<<.<<<....<<<<<....>>>>>...>>>..>>>>>.>>>...>
>>>....>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7nhl Structural basis of ABCF-mediated resistance to pleuromutilin, lincosamide, and streptogramin A antibiotics in Gram-positive pathogens.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): T2 R23 K51 R53 G54 G55 T60 R95 R96 A97 K98 Y100 Y101
• Binding residue (residue number reindexed from 1): T1 R22 K50 R52 G53 G54 T59 R94 R95 A96 K97 Y99 Y100

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7nhl, PDBe:7nhl, PDBj:7nhl
• PDBsum: 7nhl
• PubMed: 34117249
• UniProt: Q2FZ42|RL19_STAA8 Large ribosomal subunit protein bL19 (Gene Name=rplS)