Structure of PDB 7m4v Chain S Binding Site BS01

Receptor Information

>7m4v Chain S (length=91) Species: 480119 (Acinetobacter baumannii AB0057)

MNNERIYQVLKGPVFSEKAQVLGDTAGVQVFKVDINATKLEIKKAVEKLF
GVEVVKVNTTITKGKTKRFGRTLGRRSDVKKAYVTLKAGQD

Ligand information

>7m4v Chain A (length=2732)

agucaaguaauuaagugcaugugguggaugccuuggcagucagaggcgau
gaaagacgugauagccugcgaaaagcuccggggaggcggcaaauauccuu
ugauccggagaugucugaaugggggaacccaccuacuuuaagguagguau
ugcaacaugaauacauaguguugcaaggcgaacgaggggaagugaaacau
cucaguacccuuaggaaaagaaaucaauugagauucccucaguagcggcg
agcgaacggggaucagcccauuaaguuauguguguuuuaguggaacgcuc
ugggaagugcgaacguagagggugauauucccguacacgaaagggcacac
auaaugaugacgaguagggcgaggcacgugaaaccuugucugaauauggg
gggaccauccuccaaggcuaaauacuccugacugaccgauagugaaccag
uaccgugagggaaaggcgaaaagaaccccugugaggggagugaaauagau
ccugaaaccgcaugcauacaagcagugggagcaccuucguggugugacug
cguaccuuuuguauaaugggucagcgacuuauauucaguagcgagguuaa
ccguauaggggagccguagagaaaucgagucuuaauagggcguuuaguug
cuggguauagacccgaaaccaggcgaucuauccaugagcagguugaaggu
uggguaacacuaacuggaggaccgaacccacugucguugaaaagccaggg
gaugacuuguggauaggggugaaaggcuaaucaagccuggugauagcugg
uucuccccgaaagcuauuuagguagcgccucggacgaauaccauaggggg
uagagcacuguuucggcaccgaugcaaacuccgaauaccuaugaguacua
uccgggagacagacugcgggugcuaacguccguagucaagaggaaaacaa
uccagaccgccagcuaaggccccaaaaucauaguuaagugggaaacgaug
ugggaaggcauagacagcuauagucgagucggccugcgcggaagauguaa
cggggcuaaaacuaugugccgaagcugcggauguauacuuuguauacgug
guaggggagcguucuguaagccgaugaagguguguugagaagcaugcugg
agguaucagaagugcgaaugcugacgugaguaacgacaaaacgggugaaa
aacccguucgccgaaagaccaaggguuccaguccaacguuaaucggggcu
gggugagucgaccccuaaggcgaggccgaaaggcguagucgaugggaaaa
ugguuaauauuccauuacuucuguguaaugcgaugagaggacggagaagg
cuaaaucagccuggcguugguuguccaggugaaaggauguaggcauguau
cuuaggcaaauccgggguacucuaugcugagaucugauagcaagcuguac
uugugcagcgaagugguugaugccaugcuuccaggaaaagucucuaagcu
ucaguuacacaggaaucguacccgaaaccgacacagguggucaggucgag
uagaccaaggcgcuugagagaacucugcugaaggaacuaggcaaaauggu
accguaacuucgggagaagguacgcuguuguuggugauggaacucgcuuc
cugagcugacgacagccgcagaaaccaggccgcugcaacuguuuauuaaa
aacauagcacucugcaaacacgaaaguggacguauagggugugaugccug
cccggugcuggaagguuaauugaugggguuagcguaagcgaagcucuuga
ucgaagccccaguaaacggcggccguaacuauaacgguccuaagguagcg
aaauuccuugucggguaaguuccgaccugcacgaauggcauaaugauggc
ggcgcugucuccagcagaggcucagugaaaucgaaaucgcugugaagaug
caguguacccgcggcuagacggaaagaccccgugaaccuuuacugcagcu
ugacacugaacuuugauugagguucuaacucugucccgugauccgggacg
aggaccgugucugguggguaguuugacuggggcggucuccuccuaaagag
uaacggaggaguacgaaggugcgcucagcguggucggaaaucacgcguag
aguauaaaggcaaaagcgcgcuuaacugcgagacccacaagucgagcagg
uacgaaaguaggucuuagugauccggugguucuguauggaagggccaucg
cucaacggauaaaagguacucuggggauaacaggcugauaccgcccaaga
guucauaucgacggcgguguuuggcaccucgaugucggcucaucucaucc
uggggcugaagcaggucccaaggguauggcuguucgccauuuaaagaggu
acgcgagcuggguuuagaacgucgugagacaguucggucccuaucuaccg
ugggcgcuggaaauuugagaggaucugcuccuaguacgagaggaccagag
uggacgaaccucugguguaccgguugugacgccagucgcaucgccgggua
gcuauguucggaagggauaaccgcugaaagcaucuaagcgggaagccuac
cucaagauaagauuucccuaggacuuuauguccucuaaagagccguucga
gacuaggacguugauagguuggauguggaagcauagugauaugugaagcu
gaccaauacuaauugcucgugaggcuugacua

<<<<<<<<......<<<<<<<<<<<.....[[..[[<<<<<<<[[<<...
...<<<<<<.[..(((.>>>.....<<<<<<<..<<.)))...]..>>..
...>>>>>>>...>>>....<<<....>>><<<<<<<....>>>>>>>.<
<<<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<<....>
>......>>>>>.>.........<<....>>...<<<<<.....<<....
.>>....>>>>>...<<<...<..<<<<<<<<<<<<<<.<<<...<<<.<
......>.>>>((......<<<.....))>>>...>>>...>>>>>>>>>
>>>>>...>.......<<<<<<<<.........>>>>>>>>......<<<
<<..<<<<>>>>>..>>>......]].>>>>>>>.]].............
..<<....>>....]].........<<<<.....>>>>............
......>>>>>>>>>>>.......<<<<..<<<<<.....>>>>>..>>>
>...<.<........>.>.<<<<<<....<<<<<<<<<<<<<..<<<...
<<.....>>...>>>...<<....>>..<<<<.....>>>>.....>>>>
>>>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<....<<<
<<<......>>>>>>.....>>>...<<<.<<<.<........>.>>>>>
>....>>>>>>>>>...<<<.....>>>......>>>>>>...>..>.>>
..<<<<<<....<<<<<....>>>>>.<<<<<<<....<<<<<<<<<...
..<<<...<<<.<<<<..>>>>.>>>..>>.>.....>>>>>>.>>>...
>>>>>>.>...<<<<<<<<<<......>>>>>>>>>>....<<<......
>>>...<<<<.<<<...<<<(((.....<<<<<<...........<<..<
<<<<..<<........<<<..>>>....>>..>>>>>.>>..........
.)))......>>>>>>.>>>...>>>>>>><<<<<<<<...>>>>>>>>.
...>>>>>><.<<<<<...<<<......<<<<<<<<....>>>>>>>>..
.>>>..>>>>>..>.....>>>>>><......<<.....<<<<<<.....
..>>>>>><<<<....<<<<<.<<...<<<<<<...<<.....>>.>>>>
>>....((<<<.<<<...<<<...<<<....>>>...>>>...>>>..<<
<<<........>>>>>))<<<<<<<<<<<<<...<<<<....<<<<..<<
<..<<<<<<<<<<<...((((...>>>>>>....<<<<...<<<<.<<<<
<<..<<.....>>.>>>>>>....>>>>...>>>>........<<<<<..
....>>>>>....>>>>>...>>>...>>>>.........>>>>...>>.
...>>>>>>>>>>>.>>>..>>.....<<......>>>>>>>.<<<<...
..>>>>..>>>>..<<<<....<<<<<<<<.<<...............<<
<<<.....<..>......>>>>><<<<<<<<<<<<...<<<<.....>>>
>...>>>>>>>>>>>>............<<<<<<<<<..<<<........
.>>>..<<<<<<<.....<<<....>>>.......>>>>>>>.<<<<<.<
<<..<<<<<<<..<<<<.......<<<<<<.<<....>>..>>>>>>...
....>>>>>>>>>..>>>>><<<<<<.......>>>>>>........<..
.........<<<<<<.....>>>>>>.....>....>>>>>..>>.>>>>
>>>.....>>.>>>>>>>>.>>>>>>.....>.....<<<<<........
>>>>>..<<<<<<<.<<<<.[[......<<<<.[[<<<<..<<<<..<<<
.<<<<<.<<<<<<<<..>>>>>>>>...<<<.<<<<<<.....>>>>>>>
>>....>>>>>.>>>...>>>>..<<<<.....>>>><<<<<<.......
....>>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>....
.<<.[.(((>>....>>>>>>>...<<<<..<<<......>>>..>>>>.
<<<....>>>.>>)))]......<<<<<<<.((..>>>>..))>>>>>>>
.............>>>>..]]>>>>.......<<<<...<<<<<<...<<
.......>>...>>>>>>....>>>.>.......<<<<<.<<<<<<..<<
<<<<<<<......>>>>>>..>>>.<<<<<.....>>>>>.....>>>>>
..>...>>>.>>....<<<<<<<....>>>.>>>>....]]..>>>>>>>
>>>>....<<<<<<<...<<<<..<<<<<<.................>>>
>>>.<<<<<...<<<<...<<<<<<<<<<<))))>>>>>>..>>>>>...
>>>>.>>>>>...<<<....<<<<<...........>>>>>....>>>.>
>>>.......>>>>>>>..<<<<<.....>>>>><....<<<<<<<<<..
.....>>>>>....<<<<<<<<..<<....<<<<<....>>>>>...>>.
..>>>>>.>>>...>>>>..>..>>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7m4v Cryo-EM Determination of Eravacycline-Bound Structures of the Ribosome and the Multidrug Efflux Pump AdeJ of Acinetobacter baumannii.
• Resolution: 2.54 Å
• Binding residue (original residue number in PDB): M1 S16 E17 K18 K39 K44 K56 N58 T59 T60 I61 K63 K65 F69 T72 S77 K80 K81 Y83
• Binding residue (residue number reindexed from 1): M1 S16 E17 K18 K39 K44 K56 N58 T59 T60 I61 K63 K65 F69 T72 S77 K80 K81 Y83

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7m4v, PDBe:7m4v, PDBj:7m4v
• PDBsum: 7m4v
• PubMed: 34044590
• UniProt: B7IA37|RL23_ACIB5 Large ribosomal subunit protein uL23 (Gene Name=rplW)