Structure of PDB 5nif Chain S Binding Site BS01

Receptor Information

>5nif Chain S (length=236) Species: 559292 (Saccharomyces cerevisiae S288C)

DRGVSTFSPEGRLFQVEYSLEAIKLGSTAIGIATKEGVVLGVEKRATSPL
LESDSIEKIVEIDRHIGCAMSGLTADARSMIEHARTAAVTHNLYYDEDIN
VESLTQSVCDLALRFGERLMSRPFGVALLIAGHDADDGYQLFHAEPSGTF
YRYNAKAIGSGSEGAQAELLNEWHSSLTLKEAELLVLKILKQVMEEKLDE
NNAQLSCITKQDGFKIYDNEKTAELIKELKEKEAAE

Ligand information

>5nif Chain 3 (length=6) Species: 4932 (Saccharomyces cerevisiae)

WRSYYA

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5nif Crystal structure of a low molecular weight activator Blm-pep with yeast 20S proteasome - insights into the enzyme activation mechanism.
• Resolution: 3.0 Å
• Binding residue (original residue number in PDB): G19 R20 E25 L28 E159 Y165
• Binding residue (residue number reindexed from 1): G11 R12 E17 L20 E145 Y151

Enzymatic activity

• Catalytic site (original residue number in PDB): E51 R53 K66 G80 S174
• Catalytic site (residue number reindexed from 1): E43 R45 K58 G72 S160
• Enzyme Commision number: 3.4.25.1: proteasome endopeptidase complex.

Gene Ontology

Molecular Function

• GO:0003674 molecular_function
• GO:0005515 protein binding

Biological Process

• GO:0006511 ubiquitin-dependent protein catabolic process
• GO:0010499 proteasomal ubiquitin-independent protein catabolic process
• GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process
• GO:0051603 proteolysis involved in protein catabolic process

Cellular Component

• GO:0000502 proteasome complex
• GO:0005634 nucleus
• GO:0005737 cytoplasm
• GO:0005839 proteasome core complex
• GO:0019773 proteasome core complex, alpha-subunit complex
• GO:0034515 proteasome storage granule

External links

• PDB: RCSB:5nif, PDBe:5nif, PDBj:5nif
• PDBsum: 5nif
• PubMed: 28733623
• UniProt: P32379|PSA5_YEAST Proteasome subunit alpha type-5 (Gene Name=PUP2)