Structure of PDB 2xct Chain S Binding Site BS01

Receptor Information

>2xct Chain S (length=666) Species: 158879 (Staphylococcus aureus subsp. aureus N315)

KLADCSSKSPEECEIFLVEGDSAGGSTKSGRDSRTQAILPLRGKILNVEK
ARLDRILNNNEIRQMITAFGTGIGGDFDLAKARYHKIVIMTDADVDGAHI
RTLLLTFFYRFMRPLIEAGYVYIAQPPTGLGEMNADQLWETTMNPEHRAL
LQVKLEDAIEADQTFEMLMGDVVENRRQFIEDNAVYRNITSEMRESFLDY
AMSVIVARALPDVRDGLKPVHRRILYGLNEQGMTPDKSYKKSARIVGDVM
GKYHPHGDSSIYEAMVRMAQDFSYRYPLVDGQGNFGSMDGDGAAAMRFTE
ARMTKITLELLRDINKDTIDFIDNYDGNEREPSVLPARFPNLLANGASGI
AVGMATNIPPHNLTELINGVLSLSKNPDISIAELMEDIEGPDFPTAGLIL
GKSGIRRAYETGRGSIQMRSRAVIEERGGGRQRIVVTEIPFQVNKARMIE
KIAELVRDKKIDGITDLRDETSLRTGVRVVIDVRKDANASVILNNLYKQT
PLQTSFGVNMIALVNGRPKLINLKEALVHYLEHQKTVVRRRTQYNLRKAK
DRAHILEGLRIALDHIDEIISTIRESDTDKVAMESLQQRFKLSEKQAQAI
LDMRLRRLTGLERDKIEAEYNELLNYISELETILADEEVLLQLVRDELTE
IRDRFGDDRRTEIQLG

Ligand information

>2xct Chain V (length=7)

gtgcggt

Receptor-Ligand Complex Structure

Structure summary

• PDB: 2xct Type Iia Topoisomerase Inhibition by a New Class of Antibacterial Agents.
• Resolution: 3.35 Å
• Binding residue (original residue number in PDB): R1033 K1043 H1046 H1079 H1081 S1085 R1092 I1175 R1272
• Binding residue (residue number reindexed from 1): R208 K218 H221 H254 H256 S260 R267 I350 R447

Enzymatic activity

• Enzyme Commision number: 5.6.2.2: DNA topoisomerase (ATP-hydrolyzing).

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003918 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
• GO:0005524 ATP binding

Biological Process

• GO:0006259 DNA metabolic process
• GO:0006265 DNA topological change

External links

• PDB: RCSB:2xct, PDBe:2xct, PDBj:2xct
• PDBsum: 2xct
• PubMed: 20686482
• UniProt: P66937|GYRB_STAAN DNA gyrase subunit B (Gene Name=gyrB);
  Q99XG5|GYRA_STAAN DNA gyrase subunit A (Gene Name=gyrA)