Structure of PDB 6lqt Chain RK Binding Site BS01

Receptor Information

>6lqt Chain RK (length=360) Species: 559292 (Saccharomyces cerevisiae S288C)

KYTTFQGSQNFRLRIVLATLSGKPIKIEKIRSGDLNPGLKDYEVSFLRLI
ESVTNGSVIEISYTGTTVIYRPGIIVGGASTHICPSSKPVGYFVEPMLYL
APFSKKKFSILFKGITASHNDAGIEAIKWGLMPVMEKFGVRECALHTLKR
GSPPLGGGEVHLVVDSLIAQPITMHEIDRPIISSITGVAYSTRVSPSLVN
RMIDGAKKVLKNLQCEVNITADVWRGENSGKSPGWGITLVAQSKQKGWSY
FAEDIGDAGSIPEELGEKVACQLLEEISKSAAVGRNQLPLAIVYMVIGKE
DIGRLRINKEQIDERFIILLRDIKKIFNTEVFLKPVDEADNEDMIATIKG
IGFTNTSKKI

Ligand information

>6lqt Chain SA (length=1155)

aagauaguuaucugguugauccugccaguagucauaugcuugucucaaag
ccaugcaugucuaaguauaagcaauuuauacagugaaacugcgaaucagu
uaucguuuauuugauagcaugguauaacugugguaauucuagagcuaaua
caugcuaaucucgacccuuuggaagagauguauuuauuagagucuucgga
cucuuugaugauucauaauaacuuuucgaaucgcauggccuugugcuggc
gaugguucauucaaauuucugcccuaucaacuuucgaugguaggauagug
gccuaccaugguuucaacggggcaggcgcgcaaauuacccaauccuaauu
cagggagguagugacaauaaauaacgauacagggcccauucgggucuugu
aauuggaaugaguacaauguaaauaccuuaacgaggaacaauuggagggc
aagucuggugccagcagccgcgguaauuccagcuccaauagcguauauug
cucguaguugaacuuugggcccgguuggccgguccggauuuccaacgggg
ccuuuccuuuuuacuuugaaaaaauuagaguguucaaagcaggcguauug
cucgaauauauuagcauggaauaauagaauagguugguucuauuucuagg
accugauuaauagggacggucgggggcaucaguauucaauugucagaggu
gaaauucuuggauuuauugaagacuaacuacugcgaaagcauuugccaag
gacguuuucauuaaucaagaacguagggaucgggugguguuuuuuuaaug
acccacucggcaccuugggggaguauggucgcaaggcugaaacuuaaagg
aauugacggaagggcaccaccaggaguggagccugcggcgcgcgcuacac
ugacggagccagcgaguaacuccgucgugcugacgaggaauuccuaguaa
gcgcaagucaucagcuugcguugauuacgucccugcccuuuguacacacc
gcccgucgcuaguaccgauugaauggcuuagugaggccucaggaucugcu
uagagaagggggcaacuccaucucagagcggagaauuuggacaaacuugg
ucauuuagaggaacuaaaaggguuuccguaggugaaccugcggaaggauc
auuaa

..............................<..<<<<<<...<......<
<<.<<<..<<....<<....<<..........>>...>>.>>....<<<.
.<<..<<....<<<.......<<....<<.<<<.....>>>.>>.....>
>......<<<<<..<<....>>..>>>>><...<<<<<<..<<<<..>>>
>....<<......>>>>>>>>.....>...<<<<..<<<.....>>>.>>
>>....>>>...>>>>..>>>..<<....<<.....<<<<<<<<......
.>>>>>>>>.>>......>>.>>>>>>.........<<<....<<<....
.>>>..>>>.........>.....<<<<<<<..<<<<....>>>>..>>>
>.>>>......<<..<...........>..>>.........<<<<<<...
....<<<....>>>..................>>>>>>..>>>>>>.>.<
.<<...<<<.<<.....<<<<<.<<<<<..<.<...>.>..>>>>>.>>>
>>........<<<<<.<<............<.......>...<<.....>
>.............>>.>>..>>>..........<..<...<..>..>..
>.....>>....<<<<<<.<<...<<<<..<<..<<<<<<.<...<<<..
....>>>......>.>>>>>>..>>.......<<....>>...>>>>..>
.>>>>.>>>...>>>...>>.>..<<<..<<<<<<<<.<<<........>
>>>>>>>>>>..>>>.....................<<.........>>.
....<<<<<<<<<<<<..<<.<<<<<<..<<<.<<<<.>>>>.>>>....
.<<<<<<<...........>>>>>>>.............>>>>>>....<
<<<<<<<.......>>>>>>>>......>>...>>>>>>>..........
..>>>>>..<<<<.<<....<.<<<<.<..<<..<.<<......<<...<
<......<<<<....>>>>......>>...>>......>>.>..>>..>.
>>>>>....>>.>>>>.......<<<<<<<<<....>>>>>>>>>.....
.....

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6lqt Cryo-EM structure of 90 S small ribosomal subunit precursors in transition states.
• Resolution: 4.9 Å
• Binding residue (original residue number in PDB): R199 S201 R231 G232
• Binding residue (residue number reindexed from 1): R193 S195 R225 G226

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0003963 RNA-3'-phosphate cyclase activity
• GO:0004521 RNA endonuclease activity
• GO:0005515 protein binding
• GO:0008047 enzyme activator activity

Biological Process

• GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000479 endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006364 rRNA processing
• GO:0006396 RNA processing
• GO:0030490 maturation of SSU-rRNA
• GO:0042254 ribosome biogenesis
• GO:0042274 ribosomal small subunit biogenesis
• GO:2000232 regulation of rRNA processing

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0030686 90S preribosome
• GO:0032040 small-subunit processome

External links

• PDB: RCSB:6lqt, PDBe:6lqt, PDBj:6lqt
• PDBsum: 6lqt
• PubMed: 32943522
• UniProt: Q08096|RCL1_YEAST RNA 3'-terminal phosphate cyclase-like protein (Gene Name=RCL1)