Structure of PDB 7ls2 Chain R2 Binding Site BS01

Receptor Information

>7ls2 Chain R2 (length=118) Species: 10090 (Mus musculus)

KIRTSPTFRRPKTLRLRRQPKYPRKSAPRRNKLDHYAIIKFPLTTESAMK
KIEDNNTLVFIVDVKANKHQIKQAVKKLYDIDVAKVNTLIRPDGEKKAYV
RLAPDYDALDVANKIGII

Ligand information

>7ls2 Chain A2 (length=3706)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggaaaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccgcgcgucgcggcgugggaaau
guggcguacggaagacccacuccccggcgccgcucguggggggcccaagu
ccuucugaucgaggcccagcccguggacggugugaggccgguagcggccc
ccggcgcgccgggcucgggucuucccggagucggguugcuugggaaugca
gcccaaagcgggugguaaacuccaucuaaggcuaaauaccggcacgagac
cgauagucaacaaguaccguaagggaaaguugaaaagaacuuugaagaga
gaguucaagagggcgugaaaccguuaagagguaaacgggugggguccgcg
caguccgcccggaggauucaacccggcggcguccggccgugccggugguc
cggcggaucuuucccgcgcgggggaccgcccccggccggcgaccggccgc
cgccgggcgcacuuccaccguggcggugcgccgcgaccggcuccgggacg
gccgggaaggcccgguggggaagguggcucggggggggcucaccccgagu
guuacagcccuccggccgcgcuuuucgccgaaucccggggccgaggaaga
ccgucgccgcgcucucccucucccgggugggggccgggccgccccuccca
cggcgcgaccgcuccggggcggacuguccccagugcgccccgggcgucgu
cgcgccgucgggucccgggggcgucggucacgcgugacgaagccgagcgc
acggggucggcggcgaugucggcuacccacccgacccgucuugaaacacg
gaccaaggagucuaacgcgugcgcgagucaggggcucguccgaaagccgc
cguggcgcaaugaaggugaagggccccgcccgggggcccgaggugggauc
ccgaggccucuccaguccgccgagggcgcaccaccggcccgucucgcccg
ccgcgccggggagguggagcacgagcguacgcguuaggacccgaaagaug
gugaacuaugcuugggcagggcgaagccagaggaaacucugguggagguc
cguagcgguccugacgugcaaaucggucguccgaccuggguauaggggcg
aaagacuaaucgaaccaucuaguagcugguucccuccgaaguuucccuca
ggauagcuggcgcucucgcuacgcaguuuuauccgguaaagcgaaugauu
agaggucuuggggccgaaacgaucucaaccuauucucaaacuuuaaaugg
guaagaagcccggcucgcuggcguggagccgggcguggaaugcgagugcc
uagugggccacuuuugguaagcagaacuggcgcugcgggaugaaccgaac
gccggguuaaggcgcccgaugccgacgcucaucagaccccagaaaaggug
uugguugauauagacagcaggacgguggccauggaagucggaauccgcua
aggaguguguaacaacucaccugccgaaucaacuagcccugaaaauggau
ggcgcuggagcgucgggcccauacccggccgucgccggcagucggaacgg
aacgggacgggagcggcgucgggccccgcggagccuacgccgcgacgagu
aggagggccgcugcggugagccuugaagccuagggcgcgggcccgggugg
agccgccgcaggugcagaucuuggugguaguagcaaauauucaaacgaga
acuuugaaggccgaaguggagaaggguuccaugugaacagcaguugaaca
ugggucagucgguccugagagaugggcgagugccguuccgaagggacggg
cgauggccuccguugcccucggccgaucgaaagggagucggguucagauc
cccgaauccggaguggcggagaugggcgccgcgaggcguccagugcggua
acgcgaccgaucccggagaagccggcgggagccucggggagaguucucuu
uucuuugugaagggcagggcgcccuggaauggguucgccccgagagaggg
gcccgugccuuggaaagcgucgcgguuccggcggcguccggugagcucuc
gcuggcccuugaaaauccgggggagaggguguaaaucucgcgccgggccg
uacccauauccgcagcaggucuccaaggugaacagccucuggcauguugg
aacaauguagguaagggaagucggcaagccggauccguaacuucgggaua
aggauuggcucuaagggcugggucggucgcggccggcgccuagcagccga
cuuagaacuggugcggaccaggggaauccgacuguuuaauuaaaacaaag
caucgcgaaggcccgcggcggguguugacgcgaugugauuucugcccagu
gcucugaaugucaaagugaagaaauucaaugaagcgcggguaaacggcgg
gaguaacuaugacucucuuaagguagccaaaugccucgucaucuaauuag
ugacgcgcaugaauggaugaacgagauucccacugucccuaccuacuauc
cagcgaaaccacagccaagggaacgggcuuggcggaaucagcggggaaag
aagacccuguugagcuugacucuagucuggcacggugaagagacaugaga
gguguagaauaagugggaggcccggccgccggugaaauaccacuacucuc
aucguuuuuucacugacccggugaggcgggggggcgagccccgaggggcu
cucgcuucuggcgccaagcguccgucccgcgcgugcgggcgggcgcgacc
cgcuccggggacagugccagguggggaguuugacuggggcgguacaccug
ucaaacgguaacgcagguguccuaaggcgagcucagggaggacagaaacc
ucccguggagcagaagggcaaaagcucgcuugaucuugauuuucaguacg
aauacagaccgugaaagcggggccucacgauccuucugaccuuuuggguu
uuaagcaggaggugucagaaaaguuaccacagggauaacuggcuuguggc
ggccaagcguucauagcgacgucgcuuuuugauccuucgaugucggcucu
uccuaucauugugaagcagaauucaccaagcguuggauuguucacccacu
aauagggaacgugagcuggguuuagaccgucgugagacagguuaguuuua
cccuacugaugauguguuguugccaugguaauccugcucaguacgagagg
aaccgcagguucagacauuugguguaugugcuuggcugaggagccaaugg
ggcgaagcuaccaucugugggauuaugacugaacgccucuaagucagaau
cccgcccaggcggaacgauacggcagcgccgaggagccucgguuggcccc
ggauagccggguccccguccguccccgcucccgggcgucgggaccggggu
ccggugcggagagccguucgucuugggaaacggggugcggccggaaaggg
ggccgcccucucgcccgucacguugacgcacguucguguggaaccuggcg
cuaaaccauucguagacgaccugcuucugggucgggguuucguacguagc
agagcagcucccucgcugcgaucuauugaaagucagcccucgacacaagg
guuugu

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>>..>>>>>>>>>...>>>>>>>>>.......<<<<<<<........>>>
>>>>.....(((((..<<<<<<)))))..]]]..................
................<<....>>...............<<<......>>
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>>>>>>>......>.>.>>>.>>>>>...<<<<<<<.<<<<<<<<<<<<<
<<...<<.<<<<.<<..<<<......<<<<<<<<.<....>.>>>>>>>>
........>>>>>>>>>.......>>>>>>..>>>>>>>>>>>.......
........<<<<<<.<<<<..<<..>>..>>>>...<<<.<<<.<.<<<<
<<<<<<<<<<<<<..<<<<<<..............>>>>>>.>>>>..>>
>>>>>>>>.>>>....>>>..>>>>.....<<.....>>.....>>>>>>
......>>.>>>>>...>>>>>...>>>>>>>>..<<<<........>>>
><<<<<<....<<<<<<<<<<<<<..<<<...<<<<........>>>>..
...>>>...<<....>>...<<<<<<<.....>>>>>>>..<<<<<...<
<<..<<<...(((.[...>>>..>>>....>>>>>.<<<<<<<<<.<<<<
......>>>>>>>>>>>.>>....>>>>>>>>>>>>>..<<.<..<<<<<
<<.....<<<<<<<<<..<<<<...<<<<<<<....>>>>>>>...>.>>
>...<<<..<<.<<........>>>>.>>>....>>>>>>>>>...<<..
.....>>......>>>>>>...>..>.>>..<<<<<<....<.<<<....
>>>.><<<<<<<<<<.......<<<.<<<<<<<<<...<<<....<<<..
<.<<<<...<<<<.<<...>>.>>>>.>>>>.>..>>>...>>>...>>>
>>>>...<<<<<<<<<.<......>>>>>>>>>>....>>>>>>>>>>>>
.>>>..<<<<.<<<<<.....>>>>>.>>>>....<<<......>>>...
<<<<<<<...<<<(((.......<<<<<.....<<..........<<<.<
<<<<<<<<<.......<<<<<.<<<...<<.........>>...>>>...
......<<......>>...>>>>>...>>>>>>>>>>>>>..........
))).>>...>>>>>..>>>...>>>>>>><<<<<<.<<<...........
..].)))...<<.<<.>>>>..................>>>>>>>>>...
.>>>>>>...<<<<<<<..<<......<<<<.<<<<....>>>>>>>>..
.>>.>>>>>>>........>>>>>><<.....<<.....<<<<<<.....
..>>>>>>.<<<.....<<<<<.<<...<<<<<<...<<.....>>.>>>
>>>....((<<<<<<...<<....<<<....>>>..........<<.<<<
<<<<<<...>>>>>>>>>>>......>>....>>>..<<<<<........
>>>>>))<<<<....<<<<<...<<<<<<<....>>>>>>>...<<<...
.>>>...<..<<<<<<<...<<<<<<<<<<<<<.<<<<<<<<<..{{{..
>>>>>>....<<<<<.<<<<.<<<......<<<.....>>>......>>>
>>>>..>>>>>.....<<<<<<<<<..>>.>>>>>>>>>>..>.>>>>>>
>>>>>>.........>>>>>>>..>..........>>.>>>.>>>>.>>>
..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>....<<<
<.....<<<<<<<.<<.........<.....<<<<<....<<..>>....
.>>>>><<.<<<<<<<<<<<..<<<.<<..>>.>>>.......>>>>>..
>>>>>>.>>............<<<<<<<<<..<<<.........>>>..<
<<<<<<....<<<<.....>>>>......>>>>>>>...<<..<<<..<<
<<<.<<..<<<.....<<<<....>>>>.....>>>>>>>>..>>>>><<
<<<<.......>>>>>>........<...........<<<<<.......>
>>>>.....>.....>>....>>.>>>>>>>..>..>>.>>>>>>>..>>
>>>>....>>....<<<<<........>>>>>....[[[[[..{{{.{{.
.....[[[[.{{[[[[..<<<<<..<<<<<<<<.<<<<<<<<<<<<<<<<
<...........<<<<..<<<<.>>>>............>>>>..>>>>>
>>.>>>>>>>>>>...<<<<<.....<<<.<..<.<<<<.<<...>>>>>
>.>..............<<<<<<<.<<<<<....>>>>>>>>>>>>..>>
>>..>>>>>....>>>>>>>>...>>>>>...<<<.....>>>..<<<<<
<...........>>>>>>..<<.....<<<<<...<<<<<<.......>>
>>>>.....<<...(((>>....>>>>>.....<<<.<<<<<.......>
>>>.>>>>.<<<....>>>.>>))).......((((((..(((..>>>>>
>.)))))))))..........]]]]..}}]]]].......<<.<.<.<<<
<<<...<<.......>>...>>>>>>...>>.>.>.......<<<<<.<<
<<<<..<.<.<<<<<......>>>>>...>.>.<<<.........>>>..
...>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>....}}.
.}}}.]]]]].....<<<<<<....<<<..<.<<<<<.............
....>>>>>.><<<<....<<<<....<<.<<<<<<<}}}.>>>>>..>>
.>>...>>>>...>>>><<<<<...<<<<<...........>>>>>...>
>>>>.>>>.....>>>>>>.....<<<<<<.<<<..<<.<....<<<...
.....<<<<<<.<<<<<..<..<.<.........>.>....>..>>>>>>
>>>>>.......>>>...<<<..<<<<......<<.<<<<<<........
>>>>>>>>.....>>>>..>>>...............>.>>..>>>>>>>
>><<<<<.<.............>....<<.<<<<<<<.<<.....<<<<.
...<<<<<......>>>>>...>>>>....>>....>>>>.>>>.>>..>
>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7ls2 Functionally distinct roles for eEF2K in the control of ribosome availability and p-body abundance.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): K39 P44 T45 F46 R47 R48 P49 K50 T51 L52 R53 R55 R56 Q57 P58 K59 Y60 R62 T83 K89 N93 N105 K106 H107 K123 N125 T126 L127 I128 P130 K134 K135 Y137 R139
• Binding residue (residue number reindexed from 1): K1 P6 T7 F8 R9 R10 P11 K12 T13 L14 R15 R17 R18 Q19 P20 K21 Y22 R24 T45 K51 N55 N67 K68 H69 K85 N87 T88 L89 I90 P92 K96 K97 Y99 R101

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding
• GO:1904841 TORC2 complex binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0008150 biological_process
• GO:0140236 translation at presynapse
• GO:0140242 translation at postsynapse

Cellular Component

• GO:0005634 nucleus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0045202 synapse
• GO:0098793 presynapse
• GO:0098794 postsynapse
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7ls2, PDBe:7ls2, PDBj:7ls2
• PDBsum: 7ls2
• PubMed: 34815424
• UniProt: P62751|RL23A_MOUSE Large ribosomal subunit protein uL23 (Gene Name=Rpl23a)