Structure of PDB 7o72 Chain R Binding Site BS01

Receptor Information

>7o72 Chain R (length=261) Species: 559292 (Saccharomyces cerevisiae S288C)

EVFDGNDIENNETKVYEESLDLDLERSNRQVWLVRLPMFLAEKWRDRNNL
HGQELGKIRINKDGSKITLLLNENDNDSIPHEYDLELTKKVVENEYVFTE
QNLKKYQRDRYIPYVKTIPKKTAIVGTVCHECQVMPSMNDPNYHKIVEQR
RNIVKLNNKERITTLDETVGVTMSHTGMSMRSDNSNFLKVGREKAKSNIK
SIRMPKKEILDYLFKLFDEYDYWSLKGLKERTRQPEAHLKECLDKVATLV
KYTLRPEYKKL

Ligand information

>7o72 Chain N (length=65)

agcacgctgtgtatataatagctatggaacgttcgattcacctccgatgt
gtgttgtacatacat

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7o72 Structure of RNA polymerase II pre-initiation complex at 2.9 angstrom defines initial DNA opening.
• Resolution: 3.4 Å
• Binding residue (original residue number in PDB): N288 K290 S291 I292 R293 P325
• Binding residue (residue number reindexed from 1): N198 K200 S201 I202 R203 P235

Enzymatic activity

• Enzyme Commision number: 3.6.4.12: DNA helicase.

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0000993 RNA polymerase II complex binding
• GO:0003677 DNA binding
• GO:0005515 protein binding

Biological Process

• GO:0001174 transcriptional start site selection at RNA polymerase II promoter
• GO:0006355 regulation of DNA-templated transcription
• GO:0006366 transcription by RNA polymerase II
• GO:0006367 transcription initiation at RNA polymerase II promoter
• GO:0006368 transcription elongation by RNA polymerase II
• GO:0051123 RNA polymerase II preinitiation complex assembly

Cellular Component

• GO:0005634 nucleus
• GO:0005674 transcription factor TFIIF complex

External links

• PDB: RCSB:7o72, PDBe:7o72, PDBj:7o72
• PDBsum: 7o72
• PubMed: 34133942
• UniProt: P41896|T2FB_YEAST Transcription initiation factor IIF subunit beta (Gene Name=TFG2)