Structure of PDB 6em5 Chain R Binding Site BS01

Receptor Information

>6em5 Chain R (length=120) Species: 559292 (Saccharomyces cerevisiae S288C)

ANLRTQKRLAASVVGVGKRKVWLDPNETSEIAQANSRNAIRKLVKNGTIV
KKAVTVHSLPSQVVWIRRLRVLRRLLAKYRDAGKIDKHLYHVLYKESKGN
AFKHKRALVEHIIQAKADAQ

Ligand information

>6em5 Chain 1 (length=2438)

guuugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaau
aagcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaa
gcggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguugua
auuuggagagggcaacuuuggggccguuccuugucuauguuccuuggaac
aggacgucauagagggugagaaucccguguggcgaggagugcgguucuuu
guaaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugg
gugguuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaagu
acagugauggaaagaugaaaagaacuuugaaaagagagugaaaaaguacg
ugaaauuguugaaagggaagggcauuugaucagacaggccagcaucaguu
uugguggcaggauaaauccauaggaauguagcuugccucgguaaguauua
uagccugugggaauacugccagcugggacugaggacugcgacguaaguca
aggaugcuggcauaaugguuauaugccgcccgucuugaaacacggaccaa
ggagucuaacgucuaugcgaguguuuggguguaaaacccauacgcguaau
gaaagugaacguagguuggggccucgcaagaggugcacaaucgaccgauc
cugaggauggauuugaguaagagcauagcuguugggacccgaaagauggu
gaacuaugccugaauagggugaagccagaggaaacucugguggaggcucg
guucugacgugcaaaucgaucgucgaauuuggguauaggggcgaaagacu
aaaaccaucuaguagcugguuccugccgaaguuucccucaggauagcaga
agcucguaucaguuuuaugaauguaagaaugaagagcuuuuagugggcca
uuuuugguaagcagaacuggcgaugcgggaugaaccgaacguagaguuaa
ggugccggaauacacgcucaucagacaccacaaaagguguuaguucaucu
agacagccggacgguggccauggaagucggaauccgcuaaggagugugua
acaacucaccggccgaaugaacuagcccugaaaauggauggcgcucaagc
guguuaccuauacucuaccgucaggguugauaugaugcccugacgaguag
gcaggcguggaggucagugacgaagccuagaccguaaggucgggucgaac
ggccucuagugcagaucuuggugguaguagcaaauauucaaaugagaacu
uugaagacugaaguggggaaagguuccacgucaacagcaguuggacgugg
guuagucgauccuaagagauggggaagcuccguuucaaaggccugauuuu
augcaggccaccaucgaaagggaauccgguuaagauuccggaaccuggau
auggauucuucacgguaacguaacugaauguggagacgucggcgcgagcc
cugggaggaguuaucuuuucuucuuaacagcuuaucaccccggaauuggu
uuauccggagauggggucuuauggcuggaagaggccagcaccuuugcugg
cuccggugcgcuugugacggcccgugaaaauccacaggaaggaauaguuu
ucaugccaggucguacugaggucuccaaggugaacagccucuaguugaua
gaauaauguagauaagggaagucggagggucggacgaucaacuuagggaa
ucugaacgagauucccacugucccuaucuacuaucuagcgaaaccacagc
caagggaacgggcuuggcagaaucagcggggacccuguugagcuugacuc
uaguuugacauuauugucagguggggaguaaaguuaccacagggauaacu
ggcuuguggcagucaagcguucauagcgacauugcucggcucuuccuauc
auaccgaagcagaauucgguaagcguuggauuguucacccacuaauaggg
aacgugagcuggguuuagaccgucgugagacagguuaguuuuacccuacu
gaugaauguuaccgcaauaguaauugaacuuaguacgagaggaacaguuc
auucggauaauugguuuuugcggcugucugaucaggcauugccgcgaagc
uaccauccgcuggauuauggcugaacgccucuaagucagaauccaugcua
gaacgcggugaugcuccacacagauggauacgaauaaggcguccuugugg
cgucgcugaaccauagcaggcuagcaacggugcacuuggcggaaaggccu
ugggugcuugcuggcgaauugcaaugcguggggauaaaucauuuguauac
gacuuagauguacaacgggguauuguaagcaguagaguagccuuguuguu
acgaucugcugagauuaagccuuuguugucugauuugu

..........................<<<<<<.....<......>.....
.>>>>>.>...<.....<<....>>..<<<<<......<<.....>>...
..>>>>>..<<<..<........<<.<<<<<<...>>>>>>.>>......
..>....<<<<<<<.<<<<<<<<<<<<<<<<<<<<<....<<<<......
>>>>.(.......<<<......)>>>..>.>>>>>>>>..>>>>>>>>..
..>>>>>>>>>>>........<<<<<<<........>>>>>>>.....<<
<<<<..>>>>>>..>>>....>............................
.<<....>>...............<<<<....>>>>..............
....................<<<<<.<<<<<<.....<<<<<<<.<<<<<
<<<<<<<<<<.....<<<<<<<<<......<<<<<<....>>>>>>....
...>>>>>>>.>.>.>>>>>.>>>>>>>>>>........<<<....>>>.
....>>>>>>>....>>>>>>.>>>>>..<<<<.<....>.>>>><<<<<
<...<<<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>>...<<
....>>.......<<<<<..<<<<<....>>>>>...>>>>>.<<.<<<<
<.......>>>.>>..>>....>>>>>>.>>>>>>>.<<.<..<<<<<<<
...<<<<<<<<<....<<<....<<<<<<<....>>>>>>>.....>>>.
<.<<.<<........>>>>.>........>>>>>>>>>.<<<....>.>>
...>>>>>>>.....>.>>..<<<<<<....<............><<<<<
<<<<....<<<.<<<<<<<..>>>>>.>>>>>..>>>>>>>.>>..<<<<
.<<<<<.....>>>>>.>>>>....<<<......>>>...<<<<<<<..<
<<<(((.....<<<<<<.....<<..........<<<.<<<<<<<<<<..
.....<<<<<.<<....<<.........>>....>>.........<<...
...>>...>>>>>...>>>>>>>>>>>>>..........))).>>...>>
>>>>.>>>>..>>>>>>><<<<<<<<<.........>>>>>>>>>....>
>>>>><.<<<<<<<<.<<......<<<<.<<<<....>>>>>>>>...>>
>>>>>>>>..>.....>>>>>><<.....<<.....<<<<<<.......>
>>>>><<<<.....<<<<<.<<...<<<<<<...<<.....>>.>>>>>>
....((<<<<<<...<<....<<<....>>>........<<<<<<.....
...>>>>>>....>>....>>...<<<<<........>>>>>))<<<<.<
<<<<<<.....<<<....>>>.......<<<<<<...<<<<.<<<<<<<<
<<<<<<<<<<...((..>>>>>><<..<<<<<....<<<<<<....<<<.
....>>>....>>>>>>.....>>>>>>...><<<<<<<......>>>>>
>>>>>>.>.>>>>>>>.>>>>.........>>>>>>..........>.>>
.>>>>>>>>>>>>..>>....>>>>>.<<<<.....>>>>..>>>>..<<
<<.....<<<<<<<.<<.........<<<<<<..>>>>>...>..<<<<<
<<<....>.>>>>>>>.....>>.>>>>>>>..>>>>>>....>>....<
<<<<........>>>>>....<<<<<..<<..<<<<<.<<<<<<<.<<<<
..<<<<<<<<....>>>>>>>>...>>>>.>>>>>..>>>>>>>......
........<<<<<...<<.......>>...>>>>>..<<<..<<<<<..<
.<<<<<<<......>>>>>>>...>.<<<.........>>>.....>>>>
>......>>>.......<<<<<<<....>>>.>>>>.........>>.>>
>>>....<<<<<<<<..<<<<.<.<<<<<<...............>>>>>
>.><<<<<...<<<<...<<<<<<<<<<<))..>>>>>..>>>>>>..>>
>>..>>>>>.<<<<...<<<<<...........>>>>>...>>>>.>>>>
....>>>>>>>>.<<<<<<......<<..........<<<<<<.....>>
>>>>......>>.......<<<<<<...<<<<<.<..<<<......>>>.
.>.>>>>>.>>>>>>............>>>>>>.<<<<<<..........
...........<<<<<<<<<.<<<....<<<.....<<<<........>>
>>....>>>...>>>....>>>>.>>>>>..>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6em5 Visualizing the Assembly Pathway of Nucleolar Pre-60S Ribosomes.
• Resolution: 4.3 Å
• Binding residue (original residue number in PDB): A2 N3 L4 G18 K19 L24 S37 R38 V55 V57 H58 S59 W95 I96 K117 H118 Y120 H121 K125 K128 G129 N130
• Binding residue (residue number reindexed from 1): A1 N2 L3 G17 K18 L23 S36 R37 V54 V56 H57 S58 W65 I66 K87 H88 Y90 H91 K95 K98 G99 N100

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6em5, PDBe:6em5, PDBj:6em5
• PDBsum: 6em5
• PubMed: 29245012
• UniProt: P0CX82|RL19A_YEAST Large ribosomal subunit protein eL19A (Gene Name=RPL19A)