Structure of PDB 4wf9 Chain R Binding Site BS01

Receptor Information

>4wf9 Chain R (length=101) Species: 93061 (Staphylococcus aureus subsp. aureus NCTC 8325)

HIKKGDNVKVIAGKDKGKEGKVIATLPKKDRVVVEGVNIMKKHQKPTQLN
PEGGILETEAAIHVSNVQLLDPKTNEPTRVGYKFVDGKKVRIAKKSGEEI
K

Ligand information

>4wf9 Chain X (length=2712)

uuaaguuauuaagggcgcacgguggaugccuuggcacuagaagccaauga
aggacguuacuaacgacgauaugcuuuggggagcuguaaguaagcuuuga
uccagagauuuccgaauggggaaacccagcaugaguuaugucauguuauc
gauauguauacauagcauaucagaaggcacacccggagaacugaaacauc
uuaguacccggaggaagagaaagaaaauucgauucccuuaguagcggcga
gcgaaacgggaagagcccaaaccaacaagcuugcuuguugggguuguagg
acacucuaugaguuacaaaggacgacauuagacgaaucaucuggaaagau
gaaucaaagaagguaauaauccuguagucgaaaauguugucucucuugag
uggauccugaguacgacggagcacgugaaauuccgucggaaucugggagg
accaucuccuaaggcuaaauacucucuagugaccgauagugaaccaguac
cgugagggaaaggugaaaagcaccccggaaggggagugaaauagaaccug
aaaccgugugcuuacaaguagucagagcccguuaaugggugauggcgugc
cuuuuguagaaugaaccggcgaguuacgauuugaugcaagguuaagcagu
aaauguggagccguagcgaaagcgagucugaauagggcguuuaguauuug
gucguagacccgaaaccaggugaucuacccuuggucagguugaaguucag
gcugaauggaggaccgaaccgacuuacguugaaaagugagcggaugaacu
gaggguagcggagaaauuccaaucgaaccuggagauagcugguucucucc
gaaauagcuuuagggcuagccucaagugaugauuauuggagguagagcac
uguuuggacgaggggguuaccgaauucagacaaacuccgaaugccaauua
auuuaacuugggagucagaacaugggugauaagguccguguucgaaaggg
aaacagcccagaccaccagcuaaggucccaaaauauauguuaaguggaaa
aggauguggcguugcccagacaacuagcucacuagucgagugacacugcg
ccgaaaauguaccggggcuaaacauauuaccgaagcuguggauuguccuu
ggacaaugguaggagagcguucuaagggcguugaagcaugaucguaagga
cauguggagcgcuuagaagugagaaugccggugugaguagcgaaagacgg
gugagaaucccguccaccgauugacuaagguuuccagaggaaggcucguc
cgcucuggguuagucggguccuaagcugaggccgacaggcguaggcgaug
gauaacagguugauauuccuguaccaccuauaaucguaucgaugggggga
cgcaguaggauaggcgaagcgugcgauuggauugcacgucuaagcaguag
gcugaguauauccgguacucguggcugagcugugauggggagaagaaguc
guugauuucacacugccgagaaaagccucuagauagaauaggugcccgua
ccgcaaaccgacacagguagucaagaugagaauucuaaggugagcgagcg
aacucucguuaaggaacucggcaaaaugaccccguaacuucgggagaagg
ggugcucuuuaggguuaacgcccagaagagccgcagugaauaggcccaag
cgacuguuuaucaaaaacacaggucucugcuaaaccgaaggugauguaua
ggggcugacgccugcccgugcuggaagguuaagaggagugguuagcugcg
aagcuacgaaucgaagccccaguaaacggcggccccuaagguagcgaaau
uccuugucggguaaguuccgacccgcacgaaaggcguaacgauuugggca
cugucucaacgagagacucggugaaaucauaguaccugugaagaugcagg
uuacccgcgacaggacggaaagaccccguggagcuuuacuguagccugau
auugaaauucggcuuucuaacccgcaccacuuaucguggugggagacagu
gucaagcgggcaguuugacuggggcggucgccuccuaaaagguaacggag
gcgcucaaagguucccucagaaugguugaaaucauucauagaguguaaag
gcauaagggagcuugacugcgagaccuacaagucgagcagggucgaaaga
cggacuuagugauccggugguuccgcauggaagggccaucgcucaacgga
uaaaagcuaccccggggauaacaggcuuaucucccccaagaguucacauc
gacggggagguuuggcaccucgaugucggcucaucgcauccuggggcugu
agucggucccaaggguugggcuguucgcccauuaaagcgguacgcgagcu
ggguucagaacgucgugagacaguucggucccuauccgucgugggcguag
gaaauuugagaggagcuguccuuaguacgagaggaccgggauggacauac
cucugguguaccaguugucgugccaacggcauagcuggguagcuaugugu
ggacgggauaagugcugaaagcaucuaagcaugaagccccccucaagaug
agauuucccaacuugguuauaagaucccucaaagaugaugagguuaauag
guucgagguggaagcauggugacauguggagcugacgaauacuaaucgau
cgaagacuuaau

<<<<<<......<<<<<<<<<<<.....[[..[[<<<<<<<[[<<.....
.<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>.....
>>>>>>>...>>>....<<<....>>>.<<<<<<......>>>>>>..<<
<<<<<<<.......>>>>>>>.>>.>>....<<<<<......<<....>>
.......>>>>.>.........<<....>>..<<<<<......<<.....
>>.....>>>>>..(((....<<<<<<<<....>>>>>>>>......<<<
<<<<<<...<..........<.<<<<<<<.<<<...<<<<<......>>>
>>((......<<<.....))>>>...>>>...>>>>>>>..>...>.>>>
>>..>>>>.....<<<<<<<<.........>>>>>>>>......<<<<<.
.<<<<>>>>>..)))......]].>>>>>>>.]]...............<
<....>>....]].........<<<<....>>>>................
..>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>>>...<
.<........>.>.<<<<<<....<<<<<<<.<<<<<..<<<...<<<..
...>>>...>>>...<<....>>..<<<<.....>>>>.....>>>>>.>
>>>>>>..<<.<...<<<<<<.....<<<<<<<<<..<<<....<<<<<<
.>>>>>>.....>>>...<<<.<<<<<........>>>>>>>>....>>>
>>>>>>...<<<.....>>>......>>>>>>......>.>>..<<<<<<
....<<<<<....>>>>><<<<<<<<<...<<<<<<<<<.....<<<...
<<<<<<<....<<......>>....>>>>>>>..>>.>.....>>>>.>>
>>>..>>>>>>>.>>..<<<<<<<<<<......>>>>>>>>>>....<<<
......>>>...<<<<.<<<...<<<(((.....<<<<<<..........
.<<..<<<<.<<<<........<<<<......>>>>....>>>>.>>>>.
>>...........))).....>>>>>>.>>>...>>>>>>><<<<<<<..
>>>>>>>....>>>>>>..<<<<<<<.<<......<<<<<.<<....>>.
>>>>>...>>.>>>>>>>........>>>>>><......<<....<<<<<
<.......>>>>>><<<<....<<<<<.<<...<<<<<<...<<.....>
>.>>>>>>....((<<<<.<<.<.<<<...<<<....>>>...>>>..>>
>...<<<<<........>>>>>))<<<<<<<......<<<...<<<<...
.<<<<...<<...<......<<<...{.{...>>>...<....<<<<<.<
<<<<<..<.......>..>>>..>>>..>>>>>.....>...........
..>....>>...>>>>.........>>>.>.>>>...>>>>>>>>>>>..
>>.....<<......>>>>>>>.<<<<....>.>>>..>>>>..<<<<..
..<<<<<<<<.<<...............<<<<<.....<..>......>>
>>><<<<<<<.<<<<....>>>>.>>>>>>>............<<<<<<<
<<..<<<.........>>>..<<<<<<<.....<<<...>>>.......>
>>>>>>.<<<<<..<<.<<<<<<<..<<<...<<...<<<<<..<<.>>.
..>>>>>...>>...>>>>>>>>..>>>>..............<......
.....<<<<<<.....>>>>>>.....>....>>>>>..>>.>>>>>>>.
....>>.>>>>>>>>.>>>>>>.....>.....<<<<<........>>>>
>..<<<<<<<<.(((.[[......<<<<.[[<<<<..<<<<<.<<.<<<<
<<.<<<<.<..>.>>>>....<<.<<<<<<.....>>>>>>>>.....>>
>>>>.>>..>>>>>..<<<<.....>>>><<<<<<...........>>>>
>>..<<...<<<<<<<...<<<<<<......>>>>>>.....<<.[.(((
>>....>>>>>>>...<<<<..<<<......>>>..>>>>.<<<....>>
>.>>)))]......<<<<<<<.((..>>>>..))>>>>>>>.........
....>>>>..]]>>>>.......<<<<.<.<<<<<<...<<.......>>
...>>>>>>...>>>>.>.......<<<<<.<<<<<<..<<<<<<<<<..
....>>>>>>>..>>.<<<<<.....>>>>>.....>>>>>..>...>>>
.>>....<<<<<<<....>>>.>>>>....]]..)))>>>>>>>>....<
<<<<<<...<<<<..<<<<<<<...............>>>>>>>.<<<<<
...<<<<...<<<<<<<<<<..}.}.>>>>>..>>>>>...>>>>.>>>>
>...<<<....<<<<<...........>>>>>....>>>.>>>>......
.>>>>>>>.<<<<.>>>>....<<<<<<<<<.......>>>>>....<<<
<<<<<.<<<....<<<<<....>>>>>...>>>..>>>>>.>>>...>>>
>....>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4wf9 Structural insights into species-specific features of the ribosome from the pathogen Staphylococcus aureus.
• Resolution: 3.427 Å
• Binding residue (original residue number in PDB): I3 A13 G14 K15 T26 P28 M41 K42 K43 H44 V81
• Binding residue (residue number reindexed from 1): I2 A12 G13 K14 T25 P27 M40 K41 K42 H43 V80

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:4wf9, PDBe:4wf9, PDBj:4wf9
• PDBsum: 4wf9
• PubMed: 26464510
• UniProt: Q2FW17|RL24_STAA8 Large ribosomal subunit protein uL24 (Gene Name=rplX)