Structure of PDB 8s8j Chain Q Binding Site BS01

Receptor Information

>8s8j Chain Q (length=141) Species: 284590 (Kluyveromyces lactis NRRL Y-1140)

TVPSVQTFGKKKSATAVAHVKAGKGLIKVNGSPITLVQPEILRFKVYEPL
LLVGLDKFANIDIRVKVTGGGHVSQVYAIRQAIAKGLVAYHQKFVDEQSK
NELKKAFTSYDRTLLIADSRRPEPKKFGGRGARSRFQKSYR

Ligand information

>8s8j Chain 2 (length=1798)

uaucugguugauccugccaguagucauaugcuugucucaaagauuaagcc
augcaugucuaaguauaagcaauuuauacagugaaacugcgaauggcuca
uuaaaucaguuaucguuuauuugauaguuccuuuacuacauggauaucug
ugguaauucuagagcuaauacaugcuuaaaaucucgacccuuuggaagag
auguauuuauuagauaaaaaaucaaugucuucggacuccuugaugauuca
uaauaacuuuucgaaucgcauggccuugugcuggcgaugguucauucaaa
uuucugcccuaucaacuuucgaugguaggauaguggccuaccaugguuuc
aacggguaacggggaauaaggguucgauuccggagagggagccugagaaa
cggcuaccacauccaaggaaggcagcaggcgcgcaaauuacccaauccua
auucagggagguagugacaauaaauaacgauacagggcccauucgggucu
uguaauuggaaugaguacaauguaaauaccuuaacgaggaacaacuggag
ggcaagucuggugccagcagccgcgguaauuccagcuccaguagcguaua
uuaaaguuguugcaguuaaaaagcucguaguugaacuuugggucugguug
uccgguccgacuuuaugucgcgcacugguuuucaaccggaucuuuccuuc
uggcuaaccuguacuccuugugggugcaggcgaaccaggacuuuuacuuu
gaaaaaauuagaguguucaaagcaggcgaaagcucgaauauauuagcaug
gaauaauggaauaggacguuugguucuauuuuguugguuucuaggaccau
cguaaugauuaauagggacggucgggggcaucaguauucaauugucagag
gugaaauucuuggauuuauugaagacuaacuacugcgaaagcauuugcca
aggacguuuucauuaaucaagaacgaaaguuaggggaucgaagaugauca
gauaccgucguagucuuaaccauaaacuaugccgacuagggaucgggugg
uguuuuucuuaugacccacucggcaccuuacgagaaaucaaagucuuugg
guucuggggggaguauggucgcaaggcugaaacuuaaaggaauugacgga
agggcaccaccaggaguggagccugcggcuuaauuugacucaacacgggg
aaacucaccagguccagacacaauaaggauugacagauugagagcucuuu
cuugauuuugugggugguggugcauggccguucuuaguugguggagugau
uugucugcuuaauugcgauaacgaacgagaccuuaaccuacuaaauaggg
uugcuggcacuugccgguugacucuucuuagagggacuaucgguuucaag
ccgauggaaguuugaggcaauaacaggucugugaugcccuuagacguucu
gggccgcacgcgcgcuacacugacggagccagcgaguacaaccuuggccg
agaggucuggguaaucuugugaaacuccgucgugcuggggauagagcauu
guaauuauugcucuucaacgaggaauuccuaguaagcgcaagucaucagc
uugcguugauuacgucccugcccuuuguacacaccgcccgucgcuaguac
cgauugaauggcuuagugaggccucaggauuugcuuagagaagggggcaa
cuccaucucagagcgaagaaucuggucaaacuuggucauuuagaggaacu
aaaagucguaacaagguuuccguaggugaaccugcggaaggaucauua

...<<<<..{.[[[[.>>>><<<<.<<<<<<...<............<<<
.<<<..<<....<<....<<..........>>...>>.>>......<<..
......<<<..<<..<<....<<<................<<.....<<.
<<.......>>.>>......>>........<<<<<..<<....>>..>>>
>><...<<<<<<......(((((((.<<<....>>>....<<......>>
>>>>>>.....>...<<<<..<<<.....>>>.>>>>....>>>...>>>
>..>>>.<<<....<<<....<<<<<<<<.......>>>>>>>>>>>...
...>>>...<<<.<<<<....>>>>....>>>.>>.<<.<<<........
..>>>.>>.<.<<....>>.>...>>>>>>.........<<<....<<<.
....>>>..>>>.........>.....<<<<<<<<<.<.......>..>>
>>>>.>>>......<<..<...........>..>>.........<<<<<(
(......<<<<.....<<..))>>.......>>>>.>>>>>..>>>>>>>
>>>.........<<<[[.....<.<<...<<<.<<....<.<<<<<<<<<
..<<<..<<<.......>>>....>>>.....>>>>>>>>>.>.....<<
<<<....<.<...<<.......>>...>.>....>>>.>>...<<<<<.<
<.......<<...<.......>..<<<....>>>....>>......>>.>
>..>>>.........<<<..<<<<<<<<.....))))))).>>>>>>>>.
>>>.....>>....<<<<<<.<<...<<<<..<<<.<<<<<<.<...<<<
......>>>......>.>>>>>>.>>>.......<<....>>...>>>>.
.>>.>>>.>>>...>>>...>>.>....<<<<<<<...<...<<<<....
.....>>>>...>>>>>>>>..........<<<.<<.<<<..<<<<<<<<
.<<<........>>>>>>>>>>>..>>>...<<..]]>>...>>.....>
>>.>>>.<<<......<<<......>>>....>>>..]]]].}<<<<<.<
<<<<<<..<<.<<<<<<..<<<.<<<<<<......<<........>>...
.......<<<<<.<....<<<<<........<<.<<<........>>>.>
>......>>>>>...<<.<<<..<<.<<<<<<....<<<.<<<<<....>
>>...<<<......>>>...>>.>>>....<<<<<.<<..<<<<..<<<<
<<<<<<<<....>>>>>>.>>>>>>..>>>>.>>....<<<<<<.....>
>>>>>.......>>>>>....>>>.>>>.....>>>>>>>.....>.>>>
>>...>>.>>>>.>>>.....<<<<<<<......<<.....<<<.<<<<.
...>>>>.>>>....>>.......>>>>>>>......<....<<<<<<..
........>>>>>>....>.....>>>>>>....<<<<<<<<.......>
>>>>>>>......>>...>>>>>>>>>>.>>....<..<<.<..<<<<.<
<....<<<<<<<<.<<<..<<<<..<...<<<<<<..<............
.......>...>>>>>>...>..>>>>..>>>.>>>>>>>>...>>.>>>
>...>.>>...>.....<<<<<<<<<....>>>>>>>>>.........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8s8j Structural basis of AUC codon discrimination during translation initiation in yeast.
• Resolution: 4.7 Å
• Binding residue (original residue number in PDB): Q8 T9 F10 K13 K14 S15 H21 L28 G33 E42 R66 K68 T70 G71 G72 G73 H74 V75 S76 Q77 K87 R122 R123 E125 P126 K127 K128 F129 R132 G133 A134 R135 S136 R137 Q139 K140 S141 Y142 R143
• Binding residue (residue number reindexed from 1): Q6 T7 F8 K11 K12 S13 H19 L26 G31 E40 R64 K66 T68 G69 G70 G71 H72 V73 S74 Q75 K85 R120 R121 E123 P124 K125 K126 F127 R130 G131 A132 R133 S134 R135 Q137 K138 S139 Y140 R141

External links

• PDB: RCSB:8s8j, PDBe:8s8j, PDBj:8s8j
• PDBsum: 8s8j
• PubMed: 39193907
• UniProt: Q875N2|RS16_KLULA Small ribosomal subunit protein uS9 (Gene Name=RPS16)