Structure of PDB 8s8f Chain Q Binding Site BS01

Receptor Information

>8s8f Chain Q (length=141) Species: 284590 (Kluyveromyces lactis NRRL Y-1140)

TVPSVQTFGKKKSATAVAHVKAGKGLIKVNGSPITLVQPEILRFKVYEPL
LLVGLDKFANIDIRVKVTGGGHVSQVYAIRQAIAKGLVAYHQKFVDEQSK
NELKKAFTSYDRTLLIADSRRPEPKKFGGRGARSRFQKSYR

Ligand information

>8s8f Chain 2 (length=1789)

uaucugguugauccugccaguagucauaugcuugucucaaagauuaagcc
augcaugucuaaguauaagcaauuuauacagugaaacugcgaauggcuca
uuaaaucaguuaucguuuauuugauaguuccuuuacuacauggauaucug
ugguaauucuagagcuaauacaugcuuaaaaucucgacccuuuggaagag
auguauuuauuagauaaaaaaucaaugucuucggacuccuugaugauuca
uaauaacuuuucgaaucgcauggccuugugcuggcgaugguucauucaaa
uuucugcccuaucaacuuucgaugguaggauaguggccuaccaugguuuc
aacggguaacggggaauaaggguucgauuccggagagggagccugagaaa
cggcuaccacauccaaggaaggcagcaggcgcgcaaauuacccaauccua
auucagggagguagugacaauaaauaacgauacagggcccauucgggucu
uguaauuggaaugaguacaauguaaauaccuuaacgaggaacaacuggag
ggcaagucuggugccagcagccgcgguaauuccagcuccaguagcguaua
uuaaaguuguugcaguuaaaaagcucguaguugaacuuugggucugguug
uccgguccgcgcgcacugguuuucaaccggaucuuuccuucuggcuaacc
uguacuccuugugggugcaggcgaaccaggacuuuuacuuugaaaaaauu
agaguguucaaagcaggcgaaagcucgaauauauuagcauggaauaaugg
aauaggacguuugguucuauuuuguugguuucuaggaccaucguaaugau
uaauagggacggucgggggcaucaguauucaauugucagaggugaaauuc
uuggauuuauugaagacuaacuacugcgaaagcauuugccaaggacguuu
ucauuaaucaagaacgaaaguuaggggaucgaagaugaucagauaccguc
guagucuuaaccauaaacuaugccgacuagggaucgggugguguuuuucu
uaugacccacucggcaccuuacgagaaaucaaagucuuuggguucugggg
ggaguauggucgcaaggcugaaacuuaaaggaauugacggaagggcacca
ccaggaguggagccugcggcuuaauuugacucaacacggggaaacucacc
agguccagacacaauaaggauugacagauugagagcucuuucuugauuuu
gugggugguggugcauggccguucuuaguugguggagugauuugucugcu
uaauugcgauaacgaacgagaccuuaaccuacuaaauaggguugcuggca
cuugccgguugacucuucuuagagggacuaucgguuucaagccgauggaa
guuugaggcaauaacaggucugugaugcccuuagacguucugggccgcac
gcgcgcuacacugacggagccagcgaguacaaccuuggccgagaggucug
gguaaucuugugaaacuccgucgugcuggggauagagcauuguaauuauu
gcucuucaacgaggaauuccuaguaagcgcaagucaucagcuugcguuga
uuacgucccugcccuuuguacacaccgcccgucgcuaguaccgauugaau
ggcuuagugaggccucaggauuugcuuagagaagggggcaacuccaucuc
agagcgaagaaucuggucaaacuuggucauuuagaggaacuaaaagucgu
aacaagguuuccguaggugaaccugcggaaggaucauua

...<<<<<.{.[[[[>>>>><<<<.<<<<<<...<.<...<......<<<
.<<<..<<....<<....<<..........>>...>>.>>......<<..
......<<<..<<..<<....<<<................<<.....<<.
<<<.....>>>.>>......>>........<<<<<..<<....>>..>>>
>><...<<<<<<......(((((((.<<<....>>>....<........>
>>>>>>.....>...<<<<..<<<.....>>>.>>>>....>>>...>>>
>..>>>.<<<....<<<....<<<<<<<<.......>>>>>>>>>>>...
...>>>...<<<.<<<<....>>>>....>>>.>>.<<.<<<........
..>>>.>>.<.<<....>>.>...>>>>>>.........<<<....<<<.
....>>>..>>>..>....>.>.....<<.<<<<<<<..........>>>
>>>>..>>......<<..<...........>..>>.........<<<<<(
(......<<<<.....<<..))>>.......>>>>.>>>>>..>>>>>>>
>>>.........<<<[[.....<.<<...<<<.<<....<<<<<<<<<<<
.<<<<<.<..>...>>>>>....>>>>>>>>>>>....<<<<<<....<.
<<.<<<.......>>>.>>.>....>>>>>>...<<<<<.<<.......<
<...<.......>..<<<....>>>....>>......>>.>>..>>>...
......<<<..<<<<<<<<.....))))))).>>>>>>>>.>>>.....>
>....<<<<<<.<<...<<<<..<<<.<<<<<<.<...<<<......>>>
......>.>>>>>>.>>>.......<<....>>...>>>>...>>>>>.>
>>...>>>...>>.>....<<<<<<<...<...<<<<.........>>>>
...>>>>>>>>..........<<<.<<.<<<..<<<<<<<<.<<<.....
...>>>>>>>>>>>..>>>...<<..]]>>...>>.....>>>.>>>.<<
<......<<<<....>>>>....>>>..]]]].}<<<<<.<<<<<<<..<
<.<<<<<<..<<<.<<<<<<......<<........>>..........<<
<<<.<....<<<<<........<<.<<<........>>>.>>......>>
>>>...<<.<<<..<<.<<<<<<....<<<.<<<<......>>...<<<.
.....>>>...>>.>>>....<<<<<.<<..<<<<..<<<<<<<<<<<<.
...>>>>>>.>>>>>>..>>>>.>>....<<<<<<.....>>>>>>....
...>>>>>....>>>.>>>.....>>>>>>>.....>.>>>>>...>>.>
>>>.>>>.....<<<<<<<......<<.....<<<.<<<<....>>>>.>
>>....>>.......>>>>>>>......<....<<<<<<..........>
>>>>>....>.....>>>>>>....<<<<<<<<.......>>>>>>>>..
....>>...>>>>>>>>>>.>>....<..<<.<..<<<<.<<....<<<<
<<<<.<<<..<<<<..<...<<<<<<........................
..>>>>>>...>..>>>>..>>>.>>>>>>>>...>>.>>>>...>.>>.
..>.....<<<<<<<<<....>>>>>>>>>.........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8s8f Structural basis of AUC codon discrimination during translation initiation in yeast.
• Resolution: 3.95 Å
• Binding residue (original residue number in PDB): Q8 T9 F10 K12 K14 S15 K26 L28 Q40 P41 I43 R66 T70 G71 G72 G73 H74 V75 S76 K87 I118 S121 R122 R123 P124 E125 P126 K127 K128 F129 R132 G133 R135 S136 F138 Q139 K140 S141 R143
• Binding residue (residue number reindexed from 1): Q6 T7 F8 K10 K12 S13 K24 L26 Q38 P39 I41 R64 T68 G69 G70 G71 H72 V73 S74 K85 I116 S119 R120 R121 P122 E123 P124 K125 K126 F127 R130 G131 R133 S134 F136 Q137 K138 S139 R141

External links

• PDB: RCSB:8s8f, PDBe:8s8f, PDBj:8s8f
• PDBsum: 8s8f
• PubMed: 39193907
• UniProt: Q875N2|RS16_KLULA Small ribosomal subunit protein uS9 (Gene Name=RPS16)