Structure of PDB 8eui Chain Q Binding Site BS01

Receptor Information

>8eui Chain Q (length=186) Species: 4896 (Schizosaccharomyces pombe)

GIDIERHHVKKSQRSKPASENVYLKLLVKLYRFLARRTDSRFNKAILKRL
FQSKTNRPPISISKIAALTSRKSASSQNKTTVVVGTVTDDERMLTVPKLS
IAALRFTKSARARILKAGGEVLTLDQLALRAPTGSNTVLVRGKKHAREAY
RHFGFGPHKHKAPYVRSEGRKFERARGRRKSRAFKV

Ligand information

>8eui Chain 1 (length=2855)

uugaccucaaaucagguaggacuacgcgcugaacuuaagcauaucaauaa
gcgcaggaaaagaaaauaaccaugauucccucaguaacggcgagugaagc
gggaaaagcucaaauuugaaaucuggcaacauuucuuuuguuguccgagu
uguaauuucaagaagcugcuuugaguguagacgaucggucuaaguuccuu
ggaacaggacgucagagagggugagaaccccgucuuuggucgauuggaua
ugccauauaaagcgcuuucgaagagucgaguuguuugggaaugcagcucu
aaaugggugguaaauuucaucuaaagcuaaauauuggcgagagaccgaua
gcgaacaaguagagugaucgaaagaugaaaagaacuuugaaaagagaguu
aaauaguacgugaaauugcugaaagggaagcauuggaaaucagucuuaua
ggucagcaucaguuuucgggggcggaaaaagaauaagggaagguggcugu
uuauagcccuuguuguaauacguccacuggggacugaggacugcggcuug
ccaaggaugcugacauaaugguuuucaauggcccgucuugaaacacggac
caaggagucuagcaucuaugcgaguguuugggugaaaaacccauccgcga
aaugaaagugaaugcaggugggaacgcccuggcgugcaccaucgaccgac
ccggaaguuugucaauggaaggguuugaguaagagcauagcuguugggac
ccgaaagauggugaacuaugccugaauagggugaagccagaggaaacucu
gguggaggcucguagagauucugacgugcaaaucgaucuucaaauuuggg
uauaggggcgaaagacuaaucgaaccaucuaguagcugguuccugccgaa
guuucccucaggauagcagaaacucagaucaguuuuaugagguaaagcga
augauuagagguaccuauucucaaacuuuaaauauguaagacgcccuugu
cgcuuaauuggacgugggccaucgaaugagaguuucuagugggccauuuu
ugguaagcagaacuggcgaugcgggaugaaccgaacgugagguuaaggug
ccggaauguacgcucaucagacaccagaaaagguguuaguucgaacuagc
ccugaaaauggauggcgcuuaagcguacuacccauaccucaccgucuggg
uuaagaagcucagacgaguaggcaggcguggagguuugugacgaagccuu
gggcgugagccugggucgaacagccucuagugcagaucuugguggaagua
gcaaauauucaaaugagaacuuugaagacugaaguggggaaagguuccau
gugaacagcaguuggacauggguuagucgauccuaagagauagggaagcu
ccguaugaaaggcacgauuuuucgugccuaucgaaagggaauccgguuaa
uauuccggaaccagaagguggaaucaacacggcaacguaaaugaaguugg
agacgucggcgggagcccugggaagaguucucuuuucuuuuuaacaaacc
acacccugaaaucgguuuauccggagcuaggguaugguguuuggaagagu
ucagcggcugaauccggugcgcucucgacggcccuugaaaauccaacgga
aguguuuucacaucuggucguacucauaaccgcagcaggucuccaaggug
aacagccucuaguugauagaacaauguagauaagggaagucggcaaaaug
gauccguaacuucgggauaaggauuggcucuaaggguaucaacuuagaac
ugguacggacaaggggaaucugacugucuaauuaaaacauagcauugcga
uggccagaaagugguguugacgcaaugugauuucugcccagugcucgaau
gucaaagugaagaaauucaaccaagcgcggguaaacggcaagguagccaa
augccucgucaucuaacuagugacgcgcaugaauggauuaacgagauucc
cacugucccuaucuacuaucuagcgaaaccacagccuggggaacgggcca
ggcaaaaucagcggggaaagaagacccuguugagcuugacucuaguuuga
cauugugaauuacuuaaucaaugaagcggaauuucuagcguuaaaguuuc
gcgaacugauccgcguugaugacauugucagguggggaguuuggcugggg
cggcacaucuguuaaaagauaacgcagguguccuaagggggacucaucga
gaaucucgaguagaauaaaaggguaaaaguccccuugauuuugauuuuca
gugugaauacaaaccaugaaaguguggccuaucgauccuuuguucccucg
aaauuugaggacagaggugccagaaaaguuaccacagggauaacuggcuu
cagccaagacguugcauucuucgaugucggcucuuccuaucauaccgaau
ucgguaagcguuggauuguucacccacuaauagggaacgugagcuggguu
uagaccgucgugagacagguuaguuuuacccuacugaugaagugucgucg
caaugguaauucaacuuaguacgggaaccguugauucagaucauugguau
uugcggcugccugacaaggcaaugccgcggagcuaucaucugccggauaa
cggcugagccagaauccgugccagaaagcgacgauuuauguauaaaaaua
gaguccuguaucguagaagaugggcgaugguugaacggaaguguuuugac
uuguccaugaaauuccagaauccauugcauacgacuuuaauguggaacgg
gguauuguaagcaguagagaucugcugagauuaagccuuuguucccaaga
uuugu

........................<<<<<<.....<......>......>
>>>>.>...<.....<<....>>..<<<<<......<<.....>>.....
>>>>>..<<<...........<<.<<<<<<<.......>>>>>>>.>>..
...........<<<<<.<<<<<..<<<<<..<<<<.<<<<.....<<<<.
.....>>>>.((......<<<.....))>>>......>>>>.>>>>..>>
>>>.....>>>>>>>>.>>........<<<<<<<........>>>>>>>.
....<<<<<..((((((>>>>>..>>>....>..................
...........<<....>>...............<<<<....>>>>....
..............................<<<<<<<<<<<.<...<..>
.<<<<<<<.<<<<<<<<<<<<<<<.....<.<<<<<<<<.....<....>
......>>>>>>>>....>.>>>>>.>>>>>>>>>>........<<<..>
>>.....>>>>>>>....>>>>>>>>>>>>..<<<<........>>>><<
<<<<....<<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>>..
.<<....>>.......<<<<<..<<<<<..>>>>>...>>>>>.<<<<<<
<<....<<<....>>>....>>>.>>>.>>....>>>>>>.>>>>>>..<
<.<...<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>
>>>.....>>>...<<<..<<.<<........>>>>.>>>....>>>>>>
>>>...<<.......>>......>>>>>>......>.>>..<<<<<<...
.<.<<<....>>>.><<<<<<<<<<...<<<.<<<<<<<.....<<<...
.<<<....<<<..>>>....>>>..>.>>.....>>>>>...<<<<.<<<
<.<......>>>>>.>>>>.....>>>>>>>>>>>>>.>>..<<<<.<<<
<<.....>>>>>.>>>>....<<<......>>>...<<<<<<<...<<<(
((.....<<<<<<......<..........<<<.<<<<<<<..>>>>>>>
>>>..........))).>....>>>>>>.>>>...>>>>>>><<<<<<<<
<......>>>>>>>>>....>>>>>><.<<<<<<<<.<<......<<<<.
<<<<....>>>>>>>>...>>>>>>>>>>..>.....>>>>>><<.....
<<.....<<<<<<.......>>>>>><<<<.....<<<<<.<<...<<<<
<....<<.....>>..>>>>>....((<<<<<<...<<....<<<....>
>>........<<<<<<......>>>>>>..>>....>>...<<<<<....
....>>>>>))<<<<..<<<<<<<....<<<....>>>.....((<<<<<
<...<<<<.<<<<<<<.<<<<<<<<<<...[[..>>>>>><...<<<<<.
..<<<<<<....<<<.....>>>....>>>>>>.....>>>>>....><<
<<<<<.>>>>>>>>>>>...>>>>>>>.>>>>.........>>>>>>...
...>>>>.>>>.>>>>>>>>..>>.....<<......>>>>>>>.<<<<.
....>>>>..>>>>..<<<<.....<<<<<<<.<<...............
<<<<<....<....>.....>>>>><<.<<<<<<<<..>>..>>>>>>.>
>............<<<<<<<<<..<<<.........>>>..<<<<<<<..
..<<<<.....>>>>......>>>>>>>...<<...<<..<<<<<<<..<
<<....<<<<<....>>>>.>...>>>>>>>>..>>>>.......<....
.......<<<<<.......>>>>>.....>.....>>....>>.>>>>>>
>.....>>.>>>>>>>..>>>>>>....>>....<<<<<........>>>
>>....<<<<<..<<<.<<......<<<<.<<<<<<..<<<<<.<<<<<<
<<..<<<...>>>...<<<<<.....<<<<..........))..<<<<<.
..>>>>>..>>>>..>>>>>.....>>>>>>>>..>>>>>...<<<....
.>>>..<<<<<<...........>>>>>>..<<...<<<<<<<...<<<<
<<.>>>>>>.....<<...(((>>....>>>>>>>...<<<.<<<<<...
....>>>>.>>>>.<<<....>>>.>>)))......<<<<<<<..<<<<.
))))))>>>>>>>>>>>.........>>>>..>>>>>>.......<<...
<<<<......>>>>..>.>.......<<<<<.<<<<<<..<.<<<<<<..
>>>>>>...>.<<<.........>>>.....>>>>>..>...>>>.>>..
..<<<<<<<....>>>.>>>>....>>..>>>.>>>>>.....<<<<<<<
<..<<<<...<<<<<..............>>>>>..<<<<<...<<<<..
..<<<<<<<<<<]]..>>>>>..>>>>>...>>>>..>>>>>.<<<<...
.<<<<.>>>>....>>>>.>>>>....>>>>>>>>......<<.......
<<.>>....>>........<<<<<....<<<<.<<<......>>>..>>>
>..>>>>>.........<<<<<.<.............>.....<<<<<<<
<<.<<<....<<<<.<....>>>>>...>>>....>>>>.>>>>>...>>
>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8eui Chromatin localization of nucleophosmin organizes ribosome biogenesis.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): G2 I3 I5 H8 H9 K11 K12 S13 R15 A19 S20 F34 R37 R38 R42 F43 K49 Q53 S54 K55 T56 S62 S64 A68 R72 K73 S74 A75 T87 D90 D91 E92 R93 T108 K109 G135 S136 N137 V141 R142 K144 K145 H146 A147 R148 E149 R152 F154 G155 P158 H161 K162 A163 P164 Y165 R167 R171 K172 F173 E174 R175 R177 R180 K181 S182 R183 A184 F185 K186
• Binding residue (residue number reindexed from 1): G1 I2 I4 H7 H8 K10 K11 S12 R14 A18 S19 F33 R36 R37 R41 F42 K48 Q52 S53 K54 T55 S61 S63 A67 R71 K72 S73 A74 T86 D89 D90 E91 R92 T107 K108 G134 S135 N136 V140 R141 K143 K144 H145 A146 R147 E148 R151 F153 G154 P157 H160 K161 A162 P163 Y164 R166 R170 K171 F172 E173 R174 R176 R179 K180 S181 R182 A183 F184 K185

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0043232 intracellular non-membrane-bounded organelle
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8eui, PDBe:8eui, PDBj:8eui
• PDBsum: 8eui
• PubMed: 36423630
• UniProt: Q10192|RL18A_SCHPO Large ribosomal subunit protein eL18A (Gene Name=rpl1801)