Structure of PDB 8c90 Chain Q Binding Site BS01

Receptor Information

>8c90 Chain Q (length=117) Species: 562 (Escherichia coli)

ARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQYAYRDRR
QRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVF
DKVAFTALVEKAKAALA

Ligand information

>8c90 Chain A (length=2100)

gguuaagcgacuaagcguacacgguggaugcccuggcagucagaggcgau
gaaggacgugcuaaucugcgauaagcgucgguaaggugauaugaaccguu
auaaccggcgauuuccgaauggggaaacccaguguguuucgacacacuau
cauuaacugaauccauagguuaaugaggcgaaccgggggaacugaaacau
cuaaguaccccgaggaaaagaaaucaaccgagauucccccaguagcggcg
agcgaacggggagcagcccagagccugaaucaguguguguguuaguggaa
gcgucuggaaaggcgcgcgauacagggugacagccccguacacaaaaaug
cacaugcugugagcucgaugaguagggcgggacacgugguauccugucug
aauauggggggaccauccuccaaggcuaaauacuccugacugaccgauag
ugaaccaguaccgugagggaaaggcgaaaagaaccccggcgaggggagug
aaaaagaaccugaaaccguguacguacaagcagugggagcacgcuuaggc
gugugacugcguaccuuuuguauaaugggucagcgacuuauauucuguag
caagguuaaccgaauaggggagccgaagggaaaccgagucuuaacugggc
guuaaguugcaggguauagacccgaaacccggugaucaccgggagauagc
ugguucuccccgaaagcuauuuagguagcgccucgugaauucaucuccgg
ggguagagcacuguuucggcaacccgaugcaaacugcgaauaccggagaa
uguuaucacgggagacacacggcgggugcuaacguccgucgugaagaggg
aaacaacccagaccgccagcuaaggucccaaagucaugguuaagugggaa
acgaugugggaaggcgucggccugcgcggaagauguaacggggcuaaacc
augcaccgaagcugcggcagcgacgcuuaugcguuguuggguaggggagc
guucuguaagccugcgaaggugugcugugaggcaugcuggagguaucaga
agugcgaaugcugacauaaguaacgauaaagcgggugaaaagcccgcucg
ccggaagaccaaggguuccuguccaacguuaaucggggcagggugagucg
accccuaaggcgaggccgaaaggcguagucgaugggaaacagguuaauau
uccuguacuugguguuacugcgaaggggggacggagaaggcuauguuggc
cgggcgacgguugucccgguuuaagcguguaggcugguuuuccaggcaaa
uccggaaaaucaaggcugaggcgugaugacgaggcacuacggugcugaag
caacaaaugcccugcuuccaggaaaagccucuaagcaucagguaacauca
aaucguaccccaaaccgacacagguggucagguagagaauaccaaggcgc
uugagagaacucgggugaaggaacuaggcaaaauggugccggcacgcuga
uauguaggugaggucccucgcggauggagcugaaaucagucgaagauacc
agcuggcgccaggcugucuccacccgagacucagugaaauugaacucgcu
gugaagaugcaguguacccgcggcaagacggaaaguccuccuaaagagua
acggaggagcacgaagguuggcuaauccuggucggacaucaggagguuag
ugcaauggcauaagccagcuugacugcgagcgugacggcgcgagcaggug
cgaaagcaggucauagugauccggugguucugaauggaagggccaucgcu
caacgucccuaucugccgugggcgcuggagaacugaggggggcugcuccu
aguacgagaggaccggaguggacgcaucacugguguucggguugucaugc
caauggcacugcccgguagcuaaaugcggaagagauaagugcugaaagca
ucuaagcacgaaacuugccccgagaugaguucucccugacccuuuaaggg
uccugaaggaacguugaagacgacgacguugauaggccggguguguaagc
gcagcgaugcguugagcuaaccgguacuaaugaaccgugaggcuuaaccu

<<<<<<<<......<<<<<<<<<<<....<<<..<<<<<<<<<<<<<...
...<<<<<<....(((.>>>.....<<<<<<<..<<<)))....>>>...
...>>>>>>>...>>>....<<<....>>><<<<<<<....>>>>>>>.<
<<<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<<....>
>......>>>>>.>.........<<....>>..<<<<<<.....<<....
.>>....>>>>>>...<<..<<<<......<<<<<<<<<<<<<.<<<...
<<<<<......>>>>>((......<<<.....))>>>...>>>...>>>>
>>>>>>>>>...>>>>..........<<<<<<.........>>>>>>...
.....<<<<<..((((>>>>>..>>.......>>.>>>>>>>.>>.....
..........<<....>>....>>.........<<<<.....>>>>....
.........>....>>>>>>>>>>>.......<<<<..<<<<........
>>>>..>>>>...<.<........>.>.<<<<<<....<<<<<<<<<<<<
<..<<<...<<.....>>...>>>...<<....>>..<<<<.....>>>>
.....>>>>>>>>>>>>>..<<.<..<<<<<<<....>>>>>>...>..>
.>>..<<<<<<....<<<<<....>>>>>.<<<<<<<<...<<<<<<<<.
<....<<<...<<<.<<<<....>>>>.>>>..>>.>.....>>>>>>.>
>>...>>>>>>>.>...<<<<<<<<<........>>>>>>>>>....<<<
......>>>...<<<<.<<<...<<<(((.....<<<<<<..........
.<<..<<<<<..<<..>>..>>>>>.>>...........))).....>>>
>>>.>>>...>>>>>>><<<<<<.<<....>>.>>>>>>....>>>>>><
.<<<<<...<<<......<<<<<<<<....>>>>>>>>...>>>..>>>>
>..>.....>>>>>><......<<.....<<<<<<.......>>>>>><<
<<....<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<
<.<<<...<<....<<<....>>>....>>...>>>..<<<<<.......
.>>>>>))<<<<<<<<<<<.<...<<<<....<<<<..<<<..<<<<<<<
<<<<....[[[...>>>>>><<..<<<<...<<<<<<<<<<<<.<<....
.>>>>>>>>>>..>>>>...>>>>...>><..<<<<<<..>>>>>>...>
>>>>>...>>>...>>>.>........>>>>...>.....>>>>>>>>>>
>.>>>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>.
.<<<<....<<<<<<<<.<<.........<.....<<<<..>>>><<<<<
<.....<<<...<<.<<....>>>>...>>>...>>>>>>..........
..<<<<..>>>>..>..>>.>>>>>>>>.>>>>>>.....>.....<<<<
<........>>>>>..<<<<<<<<.<<<.<<....<<<<<<.........
..>>>>>>..<<...<<<<<<<...<<<<<.........>>>>>.....<
<...(((>>....>>>>>>>...<<<<..<<<<....>>>>..>>>>.<<
<....>>>.>>))).......<<<<<<<.<<..))))..>>>>>>>>>..
.......>>..>>>>>>>>>>>....<<<<<<<...<<<<....<<<<<.
....<....>....>>>>>...<<<<<...<<<<...<<<<<<<<<<<]]
].>>>>>>..>>>>>...>>>>.>>>>>...<<<....<<<<<.......
....>>>>>....>>>.>>>>.......>>>>>>>..<<<<<.....>>>
>><....<<..<<<<<.......>>>>>....<<<<<<<<..<<....<<
<<<....>>>>>...>>...>>>>>.>>>.....>>..>..>>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8c90 Cryo-EM captures early ribosome assembly in action.
• Resolution: 3.15 Å
• Binding residue (original residue number in PDB): A1 R2 V3 K4 R5 G6 R10 R12 H13 K14 K18 G22 Y23 Y24 G25 A26 R27 R29 V30 Y31 R32 F35 Q36 A41 Y44 Y46 R47 D48 R49 R50 Q51 R52 K53 R54 Q55 F56 R57 W60 I61 N65 R69 Y75 S76 I79 N80 D90 R91 K92
• Binding residue (residue number reindexed from 1): A1 R2 V3 K4 R5 G6 R10 R12 H13 K14 K18 G22 Y23 Y24 G25 A26 R27 R29 V30 Y31 R32 F35 Q36 A41 Y44 Y46 R47 D48 R49 R50 Q51 R52 K53 R54 Q55 F56 R57 W60 I61 N65 R69 Y75 S76 I79 N80 D90 R91 K92

Gene Ontology

Molecular Function

• GO:0000900 mRNA regulatory element binding translation repressor activity
• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding
• GO:0070180 large ribosomal subunit rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0017148 negative regulation of translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8c90, PDBe:8c90, PDBj:8c90
• PDBsum: 8c90
• PubMed: 36797249
• UniProt: P0A7L3|RL20_ECOLI Large ribosomal subunit protein bL20 (Gene Name=rplT)