Structure of PDB 7yla Chain Q Binding Site BS01

Receptor Information

>7yla Chain Q (length=134) Species: 562 (Escherichia coli)

YVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNAKTDSIEKGLPIPV
VITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKISRAQLQ
EIAQTKAADMTGADIEAMTRSIEGTARSMGLVVE

Ligand information

>7yla Chain I (length=2876)

gguuaagcgacuaagcguacacgguggaugcccuggcagucagaggcgau
gaaggacgugcuaaucugcgauaagcgucgguaaggugauaugaaccguu
auaaccggcgauuuccgaauggggaaacccaguguguuucgacacacuau
cauuaacugaauccauagguuaaugaggcgaaccgggggaacugaaacau
cuaaguaccccgaggaaaagaaaucaaccgagauucccccaguagcggcg
agcgaacggggagcagcccagagccugaaucaguguguguguuaguggaa
gcgucuggaaaggcgcgcgauacagggugacagccccguacacaaaaaug
cacaugcugugagcucgaugaguagggcgggacacgugguauccugucug
aauauggggggaccauccuccaaggcuaaauacuccugacugaccgauag
ugaaccaguaccgugagggaaaggcgaaaagaaccccggcgaggggagug
aaaaagaaccugaaaccguguacguacaagcagugggagcacgcugcgug
ugacugcguaccuuuuguauaaugggucagcgacuuauauucuguagcaa
gguuaaccgaauaggggagccgaagggaaaccgagucuuaacugggcguu
aaguugcaggguauagacccgaaacccggugaucuagccaugggcagguu
gaagguuggguaacacuaacuggaggaccgaaccgacuaauguugaaaaa
uuagcggaugacuuguggcugggggugaaaggccaaucaaaccgggagau
agcugguucuccccgaaagcuauuuagguagcgccucgugaauucaucuc
cggggguagagcacuguuucggcaagggggucacuuaccaacccgaugca
aacugcgaauaccggagaauguuaucacgggagacacacggcgggugcua
acguccgucgugaagagggaaacaacccagaccgccagcuaaggucccaa
agucaugguuaagugggaaacgaugugggaaggcccagacagccaggaug
uuggcaagcagccaucauuuaaagaaagcguaauagcucacuggucgagu
cggccugcgcggaagauguaacggggcuaaaccaugcaccgaagcugcgg
cagcgacgcuuaugcguuguuggguaggggagcguucuguaagccugcga
aggugugcugugaggcaugcuggagguaucagaagugcgaaugcugacau
aaguaacgauaaagcgggugaaaagcccgcucgccggaagaccaaggguu
ccuguccaacguuaaucggggcagggugagucgaccccuaaggcgaggcc
gaaaggcguagucgaugggaaacagguuaauauuccuguacuugguguua
cugcgaaggggggacggagaaggcuauguuggccgggcgacgguuguccc
gguuuaagcguguaggcugguuuuccaggcaaauccggaaaaucaaggcu
gaggcgugaugacgaggcacuacggugcugaagcaacaaaugcccugcuu
ccaggaaaagccucuaagcaucagguaacaucaaaucguaccccaaaccg
acacagguggucagguagagaauaccaaggcgcuugagagaacucgggug
aaggaacuaggcaaaauggugccguaacuucgggagaaggcacgcugaua
uguaggugaggucccucgcggauggagcugaaaucagucgaagauaccag
cuggcugcaacuguuuauuaaaaacacagcacugugcaaacacgaaagug
gacguauacggugugacgccugcccggugccggaagguuaauugaugggg
uuagcgcaagcgaagcucuugaucgaagccccgguaaacggcggccgguc
cuaagguagcgaaauuccuugucggguaaguuccgaccugcacgaauggc
guaaugauggccaggcugucuccacccgagacucagugaaauugaacucg
cugugaagaugcaguguacccgcggcaagacggaaagaccccgugaaccu
uuacuauagcuugacacugaacauugagccuugauguguaggauaggugg
gaggcuuugaaguguggacgccagucugcauggagccgaccuugaaauac
cacccuuuaauguuugauguucuaacguugacccguaauccggguugcgg
acagugucugguggguaguuugacuggggcggucuccuccuaaagaguaa
cggaggagcacgaagguuggcuaauccuggucggacaucaggagguuagu
gcaauggcauaagccagcuugacugcgagcgugacggcgcgagcaggugc
gaaagcaggucauagugauccggugguucugaauggaagggccaucgcuc
aacggauaaaagguacuccggggauaacaggcugauaccgcccaagaguu
cauaucgacggcgguguuuggcaccucgaugucggcucaucacauccugg
ggcugaaguaggucccaaggguauggcuguucgccauuuaaagugguacg
cgagcuggguuuagaacgucgugagacaguucggucccuaucugccgugg
gcgcuggagaacugaggggggcugcuccuaguacgagaggaccggagugg
acgcaucacugguguucggguugucaugccaauggcacugcccgguagcu
aaaugcggaagagauaagugcugaaagcaucuaagcacgaaacuugcccc
gagaugaguucucccugacccuuuaaggguccugaaggaacguugaagac
gacgacguugauaggccggguguguaagcgcagcgaugcguugagcuaac
cgguacuaaugaaccgugaggcuuaa

..<<<<<<......<<<<<<<<<<<.....[[..[[<<<<<<<[[<<...
...<<<<<<....(((.>>>.....<<<<<<<..<<<)))....>>>...
...>>>>>>>...>>>....<<<....>>><<<<<<<....>>>>>>>.<
<<<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<<....>
>......>>>>>.>.........<<....>>..<<<<<<.....<<....
.>>....>>>>>>..(((..<<<<......<<<<<<<<<<<<<.<<<...
<<<<<......>>>>>((......<<<.....))>>>...>>>...>>>>
>>>>>>>>>...>>>>........<<<<<<<<.........>>>>>>>>.
.....<<<<<..<<<<>>>>>..)))......]].>>>>>>>.]].....
..........<<....>>....]].........<<<<.....>>>>....
..............>>>>>>>>>>>.......<<<<..<<<<<<.>>>>>
>..>>>>...<.<........>.>.<<<<<<....<<<<<<<<<<<<<..
<<<...<<.....>>...>>>...<<....>>..<<<<.....>>>>...
..>>>>>>>>>>>>>..<<.<...<<<<<<.....<<<<<<<<<..<<<<
...<<<<<........>>>>>.>...>>>...<<<.<<<<<........>
>>>>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>....
..>.>>..<<<<<<....<<<<<....>>>>>.<<<<<<<<...<<<<<<
<<<.....<<<...<<<.<<<<...<<.<<<.>>>..>>...>>>>.>>>
..>>.>.....>>>>>>.>>>...>>>>>>>.>...<<<<<<<<<<....
..>>>>>>>>>>....<<<......>>>...<<<<.<<<...<<<(((..
...<<<<<<...........<<..<<<<<..<<........<<<<<.<<.
..<<.......>>...>>........<<<......>>>..>>>>>....>
>..>>>>>.>>...........))).....>>>>>>.>>>...>>>>>>>
<<<<<<.<<....>>.>>>>>>....>>>>>>..<<<<<...<<<.....
.<<<<<<<<....>>>>>>>>...>>>..>>>>>........>>>>>><.
.....<<.....<<<<<<.......>>>>>><<<<....<<<<<.<<...
<<<<<<...<<.....>>.>>>>>>....((<<<.<<<...<<<...<<<
....>>>...>>>...>>>..<<<<<........>>>>>))<<<<<<<<<
<<<<...<<<<....<<<<..<<<..<<<<<<<<<<<....(((...>>>
>>><<..<<<<...<<<<<<<<<<<<.<<.....>>>>>>>>>>..>>>>
...>>>>...>><..<<<<<....>>>>>...>>>>>>...>>>...>>>
>.........>>>>...>>....>>>>>>>>>>>.>>>..>>.....<<.
.....>>>>>>>.<<<<.....>>>>..>>>>..<<<<....<<<<<<<<
.<<...............<<<<<....<<..>>.....>>>>><<<<<<.
....<<<...<<<<.....>>>>...>>>...>>>>>>............
<<<<<<<<<..<<<.........>>>..<<<<<<<.....<<<....>>>
.......>>>>>>>.<<<<<.<<<..<<<<<<<..<<<........<<<<
<<.<<....>>..>>>>>>........>>>>>>>>..>>>>><<<..>>>
........<...........<<<<<<.....>>>>>>.....>....>>>
>>..>>.>>>>>>>.....>>.>>>>>>>>.>>>>>>.....>.....<<
<<<........>>>>>..<<<<<<<<.(((.[[......<<<<.[[<<<<
..<<<<<.<<<.<<<<<.<<<<<<<<<<...<<............<<<<<
..<<<......<<<<<.<<....>>.>>>>>...>>>............>
>>>>...>>...>>>>>>>>>>...<<<.<<<<<<.....>>>>>>>>>.
...>>>>>.>>>..>>>>>..<<<<.....>>>><<<<<<..........
.>>>>>>.(<<...<<<<<<<...<<<<<<.......>>>>>>.....<<
)..(((>>....>>>>>>>...<<<<..<<<<....>>>>..>>>>.<<<
....>>>.>>))).......<<<<<<<.((..>>>>..))>>>>>>>...
..........>>>>..]]>>>>.......<<<<.<.<<<<<<...<<...
....>>...>>>>>>...>>>>.>.......<<<<<.<<<<<<..<<<<<
<<<<......>>>>>>>..>>.<<<<<.....>>>>>.....>>>>>..>
...>>>.>>....<<<<<<<....>>>.>>>>....]]..)))>>>>>>>
>....<<<<<<<...<<<<..<<<<<<<...............>>>>>>>
.<<<<<...<<<<...<<<<<<<<<<<))).>>>>>>..>>>>>...>>>
>.>>>>>...<<<....<<<<<...........>>>>>....>>>.>>>>
.......>>>>>>>..<<<<<.....>>>>><....<<..<<<<<.....
..>>>>>....<<<<<<<<..<<....<<<<<....>>>>>...>>...>
>>>>.>>>.....>>..>..>>>>>>

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7yla Cryo-EM structure of 50S-HflX complex
• Resolution: 2.52 Å
• Binding residue (original residue number in PDB): Y8 V9 K10 L11 Q12 P75 K92 P93 N94 R127 S128 R134 S135
• Binding residue (residue number reindexed from 1): Y1 V2 K3 L4 Q5 P68 K85 P86 N87 R120 S121 R127 S128

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding
• GO:0070180 large ribosomal subunit rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0006415 translational termination
• GO:0015968 stringent response

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7yla, PDBe:7yla, PDBj:7yla
• PDBsum: 7yla
• UniProt: P0A7J7|RL11_ECOLI Large ribosomal subunit protein uL11 (Gene Name=rplK)