Structure of PDB 7s9u Chain Q Binding Site BS01

Receptor Information

>7s9u Chain Q (length=117) Species: 1423 (Bacillus subtilis)

PRVKGGTVTRKRRKKVLKLAKGYFGSKHTLYKVANQQVMKSGNYAFRDRR
QKKRDFRKLWITRINAAARMNGLSYSRLMHGLKLSGIEVNRKMLADLAVN
DLTAFNQLADAAKAQLN

Ligand information

>7s9u Chain A (length=2436)

uuaaguuagaaagggcgcacgguggaugccuuggcacuaggagccgauga
aggacgggacgaacaccgauaugcuucggggagcuguaagcaagcuuuga
uccggagauuuccgaauggggaaacccaccacucguaauggagugguauc
cauaucugaauucauaggauaugagaaggcagacccggggaacugaaaca
ucuaaguacccggaggaagagaaagcaaaugcgauucccugaguagcggc
gagcgaaacgggaucagcccaaaccaagaggcuugccucuugggguugua
ggacacucuguacggaguuacaaaggaacgagguagaugaagaggucugg
aaaggcccgccauaggagguaacagcccuguagucaaaacuucguucucu
ccugaguggauccugaguacggcggaacacgugaaauuccgucggaaucc
gggaggaccaucucccaaggcuaaauacucccuagugaccgauagugaac
caguaccgugagggaaaggugaaaagcaccccggaaggggagugaaagag
auccugaaaccgugugccuacaaguagucagagcccguuaacgggugaug
gcgugccuuuuguagaaugaaccggcgaguuacgaucucgugcaagguua
agcagaagaugcggagccgcagcgaaagcgagucugaauagggcgcauga
guacguggucguagacccgaaaccaggugaucuacccauguccaggguga
aguucagguaacacugaauggaggcccgaacccacgcacguugaaaagug
cggggaugagguguggguaggggugaaaugccaaucgaaccuggagauag
cugguucucuccgaaauagcuuuagggcuagccucaagguaagagucuug
gagguagagcacugauuggacuaggcgaauucagucaaacuccgaaugcc
aaugacuuauccuugggagucagacugcgagugauaagauccguagucga
aagggaaacagcccagaccgccagcuaaggucccaaaguauacguuaagu
ggaaaaggauguggaguugacucugcgccgaaaauguaccggggcuaaac
guaucaccgaagcugcggacuguucuucgaacagugguaggagagcguuc
uaagggcugugaagccagaccggaaggacugguggagcgcuuagaaguga
gaaugccgguaugaguagcgaaagaggggugagaauccccuccaccgaau
gccuaagguuuccugaggaaggcucguccgcucaggguuagucgggaccu
aagccgaggccgaaaggcguaggcgauggacaacagguugauauuccugu
accaccuccucaccauuugagcaauggggggacgcaggaggauaggguaa
gcgcgguauuggauauccgcguccaagcaguuaggcugggaaauaggcaa
auccguuucccauaagccugagcugugauggcgagcgaaauauaguagcg
aaguuccugauuccacacugccaagaaaagccucuagcgaggugagaggu
gcccguaccgcaaaccgacacagguaggcgaggagagaauccuaagguga
ucgagagaacucucguuaaggaacucggcaaaaugaccccguaacuucgg
gagaaggggugcucuguuagggugcaagcccgagagagccgcagugaaua
ggcccaggcgacuguuuagcaaaaacacaggucucugcgaagccguaagg
cgaaguauaggggcugacgccuaggcgcaacgaucugggcacugucucaa
cgagagacucggugaaauuauaguaccugugaagaugcagguuacccgcg
acaggacggaaagaccccguggagcuuuacugcagccugauauugaaugu
ccuucuaacccgccgcccuuaucgggcggggagacagugucaggugggca
guuugacuggggcggucgccuccuaaaagguaacggaggcgcccaaaggu
ucccucagaaugguuggaaaucauucgcagaguguaaaggcacaagggag
cuugacugcgagaccuacaagucgagcagggacgaaagucgggcuuagug
auccggugguuccgcauggaagggccaucgcucaacggauaaaagcuacc
ccgguccgucgcgggcgcaggaaauuugagaggagcuguccuuaguacga
gaggaccgggauggacgcaccgcugguguaccaguuguucugccaagggc
aucgcuggguagcuaugugcggacgggauaagugcugaaagcaucuaagc
augaagccccccucaagaugagauuucccauuccgcaaggaaguaagauc
ccugaaagaugaucagguugauaggucugagguggaaguguggcgacaca
uggagcugacagauacuaaucgaucgaggacuuaac

<<<<<<......<<<<<<<<<<<....<<<..<<<<<<<<<<<<<.....
.<<<<<<.....((.>>>.....<<<<<<<..<<<)).....>>>.....
>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<
<<<<<<<.........>>>>>>>.>>.>>....<<<<<<.....<.....
.>......>>>>>.>.........<<....>>...<<<<......<<...
..>>.....>>>>...<<<....<<<<<<<<....>>>>>>>>.......
<<<<<<<<.....<<<<.......<<<<<<<<<<.<<<.....<<<<...
...>>>>.((......<<<.....))>>>...>>>...>>>>>>>>>>>>
>>.>>>>>..>>>......<<<<<<<<.........>>>>>>>>......
<<<<<..((((>>>>>..>>>......>>.>>>>>>>.>>..........
.....<<....>>....>>.........<<<<....>>>>..........
...>....>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>
>>...<.<........>.>.<<<<<<....<<<<<<<.<<<<<..<<<..
.<<<.....>>>...>>>...<<....>>..<<<<.....>>>>......
>>>>>.>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<...
.<<<<<<......>>>>>>.....>>>...<<<.<<<<<........>>>
>>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>...>..
>.>>..<<<<<<....<<<<<....>>>>><<.<<<<<<...<<<<<<<<
<......<<...<<<.<<.............>>.>>>..>>.......>>
>>.>>>>>..>>>>>>..>>..<<<<<<<.<<......>>.>>>>>>>..
..<<<......>>>...<<<<.<<<...<<<(((.....<<<<<<.....
......<<..<<<<<<<..>>>>>>>.>>...........))).....>>
>>>>.>>>...>>>>>>><<<<<<<...>>>>>>>....>>>>>><.<<<
<<<<.<<......<<<<<.<<....>>.>>>>>...>>.>>>>>>>..>.
....>>>>>><......<<....<<<<<<.......>>>>>><<<<....
<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<.<<.
..<<<...<<<....>>>...>>>...>>...<<<<<........>>>>>
))<<<<<.<<<<<<......<<...<<<<....<<<<..<<<..<<<.<.
<<<.<.....[....>.>>>.<...<.....<<<<<.<<<<<<<.<<...
..>>>>>>>>>....>>>>>.....>....><..<<...........>>.
..>>.>>>...>>>...>>>>.........>>>>..>>..>>>>>>>>>>
>>>>>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>.
.<<<<....<<<<<<<..<<<........<.....<<<<<....<<..>>
.....>>>>><<<<.....<<<<....>>>>....>>>>...........
.<<<<<<<<<..<<<.........>>>..<<<<<<<.....<<<....>>
>.......>>>>>>>..<<<<..>>>>...>>.>>>>>>>..>.>>>..>
>>>>>>.>>>>>>.....>.....<<<<<........>>>>>..<<<<<<
<<.<<<..............<<<<<<..<<<<<.<<<<<<<<<.<<....
...>>....<<.<<<<<.......>>>>>>>.....>>>>>>>>>..>>>
>>..<<<.......>>><<<<<<...........>>>>>>..<<.....<
<<<<....<<<<<.......>>>>>......<..[.(((.>....>>>>>
.....<<<<..<<<......>>>..>>>>.<<<....>>>.>>)))]...
...<<<<<<<.<<..))))..>>>>>>>>>.............>>>>..>
>...>>>>>>>>>>>....<<<<<<<...<<<<..<<<<<<<.....<..
..>....>>>>>>>.<<<<<...<<<<...<<<<<<<<<<<.]..>>>>>
>..>>>>>...>>>>.>>>>>...<<<.<..<<<<<...........>>>
>>...>>>>.>>>>.......>>>>>>>..<<<<....>>>>....<<<<
<<<<<.......>>>>>....<<<<<<<<.<<<....<<<<<....>>>>
>...>>>..>>>>>.>>>...>>>>....>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7s9u RbgA ensures the correct timing in the maturation of the 50S subunits functional sites.
• Resolution: 3.2 Å
• Binding residue (original residue number in PDB): P2 R3 V4 R11 R13 R14 G23 Y24 F25 S27 K33 Q37 Q38 S42 Y45 R48 D49 R51 K53 K54 R55 F57 R58 N66 R70 S77 K84 R92 K93
• Binding residue (residue number reindexed from 1): P1 R2 V3 R10 R12 R13 G22 Y23 F24 S26 K32 Q36 Q37 S41 Y44 R47 D48 R50 K52 K53 R54 F56 R57 N65 R69 S76 K83 R91 K92

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7s9u, PDBe:7s9u, PDBj:7s9u
• PDBsum: 7s9u
• PubMed: 35141754
• UniProt: P55873|RL20_BACSU Large ribosomal subunit protein bL20 (Gene Name=rplT)