Structure of PDB 7ohu Chain Q Binding Site BS01

Receptor Information

>7ohu Chain Q (length=131) Species: 559292 (Saccharomyces cerevisiae S288C)

HRTAPKSDNVYLKLLVKLYTFLARRTDAPFNKVVLKALFLSKINRPPVSV
SRIARALKQEGAANKTVVVVGTVTDDARIFEFPKTTVAALRFTAGARAKI
VKAGGECITLDQLAVRAPKGQNTLILRGPRN

Ligand information

>7ohu Chain 1 (length=1692)

guuugaccucaaaucagguaggaguacccgcugaacuuauaucaauaagc
ggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaagcgg
caaaagcucaaauuugaaaucugguaccuucggugcccgaguuguaauuu
ggagagggcacuuuggggccguuccuugucuauguuccuuggaacaggac
gucauagagggugagaaucccguguggcgaggagugcgguucuuuguaaa
gugccuucgaagagucgaguuguuugggaaugcagcucuaaguggguggu
uccaucuaaagcuaaauauuggcgagagaccgauagcgaacaaguacagu
gauggaaagaugaaaagaacuuugaaaagagagugaaaaaguacgugaaa
uuguugaaagggaagggcauuugaucagacaugguguuuuguggggaauc
ucgcauuucacugggccagcaucaguuuugguggcaggauaaauccauag
gaauguagcuugccucgguaaguauuauagccugugggaauacugccagc
ugggacugaggacugcgacguaagucaaggaugcuggcauaaugguuaua
ugccgccgucuugaaacacggaccaagggucuaacgucuaugcgaguguu
uggguguaaaacccauacgcguaaugaaagugaacguagguuggggccuc
gcaagaggugcacaaucgaccgauccugaggauggauuugaguaagagca
uagcuguugggacccgaaagauggugaaaccaucuaguacugguuccugc
cgaaguuucccucaggauagcagaagcucguaucaguuuuauuaaaauga
agagcuuuuagugggccauuuuugguaagcagaacuggcgaugcgggaug
aaccgaacguagaguuaaggugccggaauacacgcucaucagaccaaaag
guguuaguucaucuagacagccggacgguggccauggaagucggaauccg
cuaaggaguguguaacaacucaccggccgaaugaacuagcccugaauggc
gcucaagcguguuaccuauacucuaccgucaggguugauaugaugcccug
acgaguaggcaggcguggaggucagugacgaagccuagaccguaaggucg
ggucgaacggccucuagugagaucuuggugguaguagcagcgaaaccaca
gccaagggacgggcuuggcagaaucagcgggggcuuguggcagucaagcg
gcgacauugcucauaccgaagcagaauucgguaagcgucuacugaugaug
uuaccgcaauaguaauugaacuuaguacgagaggaacaguucauucggau
aauugguuuuugcggcugucugaucaggcagccgcgaagcuaccauccgc
uggauuauggcugaacgccucuaagucagauccaugcuagaacgcgguga
uuucuuugcuccacacaauauagauggauacgaauaaggcguccuugugg
cgucgcugaaccauagcaggcuagcaacggugcacuuggcggaaaggccu
ugggugcuugcuggcgaauugcaaugucauuuugcguggggauaaaucau
uuguauacgacuuagauguacaacgggguauuguaagcaguagaguagcc
ugguuacgaucugcugagauuaaccuuuguugucugauuugu

..........................<<<<<<...............>>>
>>.>.........<<....>>..<<<<<......<<.....>>.....>>
>>>..<<<..<........<<.<<<<<<...>>>>>>.>>........>.
...<<<<<<<<<<..<<<<<<<<<<<<<<<.....<<<<......>>>>.
((......<<<.....))>>>....>>>>>>>>..>>>>>>>......>>
>>>>>>>>........<<<<<<<........>>>>>>>.....<<<<<<.
.>>>>>>..>>>..................................<<..
..>>...............<<<<....>>>>...................
...............<<<<<.<<<<<.<....<<<<...<<<........
..>>>...>>>>..<<<<<<<.<<<<<<<<<<<<<<<.....<<<<<<<<
<......<<<<<......>>>>>.......>>>>>>>.>.>.>>>>>.>>
>>>>>>>>........<<<....>>>.....>>>>>>>....>>>>>>.>
>>>>.<<<<........>>>><<<<<<...<<<<<<.<<<<<<..<<<..
.<<<<.....>>>>...>>>..................<<<<<..<<<<<
....>>>>>...>>>>>.<<<<<<<<.......>>>.>>>.>>....>>>
>>>.>>>>>>..<<.<...<<<<<<...>>>>>>.....>.>>..<<<<<
<....<.<<......>>.><<<<<<<<<....<<<.<<<<...>>>>>>>
..>>>>>>>.>>..<<<<.<<<<<.....>>>>>.>>>>....<<<....
..>>>...<<<<<<<..<<<<(((.....<<<<<<......<......<<
<.<<<<<<<<<<.......<<<<<.<<....<<.........>>....>>
.........<<......>>...>>>>>...>>>>>>>>>>>>>....)))
.>....>>>>>>.>>>>..>>>>>>><<<<<<<<<.........>>>>>>
>>>....>>>>>><.<<<<<<<<.<<......<<<<.<<<<....>>>>>
>>>...>>>>>>>>>>..>....>>>>>><<.....<<.>>....>>...
.<<<<<.......>>>>>....<<<<<..<.........<<<<....<<.
>>....>>>>...<<<<<<<......>>>>>>>......>.>>>>>...<
<<<<<<<..<<<<.<.<<<<<<...............>>>>>>.><<<<<
...<<......<<<<<<<<........>>>>>>>>.....>>..>>>>>.
<<<<...<<<<<...........>>>>>..>>>>.>>>>....>>>>>>>
>.......<<<<<<.<<<.<<....<<..........<<<<<<.....>>
>>>>......>>.......<<<<<<...<<<<<.<..<<<......>>>.
.>.>>>>>.>>>>>>.............>>.>>>.>>>>>>..<<<<<.<
.............>.....<<<<<<<<<.<<<....<<<<....<<.<<.
..>>.>>...>>>>...>>>...>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7ohu Analysis of subunit folding contribution of three yeast large ribosomal subunit proteins required for stabilisation and processing of intermediate nuclear rRNA precursors.
• Resolution: 3.7 Å
• Binding residue (original residue number in PDB): K20 S21 K31 R38 P43 F44 K50 S55 K56 I57 N58 S63 S65 R66 R69 Q73 E74 K79 T86 T88 D89 D90 R92 T107 A108 Q135 N136 T137 I139 R141 R144
• Binding residue (residue number reindexed from 1): K6 S7 K17 R24 P29 F30 K36 S41 K42 I43 N44 S49 S51 R52 R55 Q59 E60 K65 T72 T74 D75 D76 R78 T93 A94 Q121 N122 T123 I125 R127 R130

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0043232 intracellular non-membrane-bounded organelle
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7ohu, PDBe:7ohu, PDBj:7ohu
• PDBsum: 7ohu
• PubMed: 34813592
• UniProt: P0CX49|RL18A_YEAST Large ribosomal subunit protein eL18A (Gene Name=RPL18A)