Structure of PDB 7as9 Chain Q Binding Site BS01

Receptor Information

>7as9 Chain Q (length=135) Species: 224308 (Bacillus subtilis subsp. subtilis str. 168)

MLLPKRVKYRREHRGKMRGRAKGGTEVHFGEFGIQALEASWITNRQIEAA
RIAMTRYMKRGGKVWIKIFPSKPYTAKPLEVRMGSGKGAPEGWVAVVKPG
KVLFEISGVSEEVAREALRLASHKLPIKTKFVKRE

Ligand information

>7as9 Chain A (length=2812)

guuaaguuagaaagggcgcacgguggaugccuuggcacuaggagccgaug
aaggacgggacgaacaccgauaugcuucggggagcuguaagcaagcuuug
auccggagauuuccgaauggggaaacccaccacucguaauggagugguau
ccauaucugaauucauaggauaugagaaggcagacccggggaacugaaac
aucuaaguacccggaggaagagaaagcaaaugcgauucccugaguagcgg
cgagcgaaacgggaucagcccaaaccaagaggcuugccucuugggguugu
aggacacucuguacggaguuacaaaggaacgagguagaugaagaggucug
gaaaggcccgccauaggagguaacagcccuguagucaaaacuucguucuc
uccugaguggauccugaguacggcggaacacgugaaauuccgucggaauc
cgggaggaccaucucccaaggcuaaauacucccuagugaccgauagugaa
ccaguaccgugagggaaaggugaaaagcaccccggaaggggagugaaaga
gauccugaaaccgugugccuacaaguagucagagcccguuaacgggugau
ggcgugccuuuuguagaaugaaccggcgaguuacgaucucgugcaagguu
aagcagaagaugcggagccgcagcgaaagcgagucugaauagggcgcaug
aguacguggucguagacccgaaaccaggugaucuacccauguccagggug
aaguucagguaacacugaauggaggcccgaacccacgcacguugaaaagu
gcggggaugagguguggguaggggugaaaugccaaucgaaccuggagaua
gcugguucucuccgaaauagcuuuagggcuagccucaagguaagagucuu
ggagguagagcacugauuggacuaggggcccggguuaccgaauucaguca
aacuccgaaugccaaugacuuauccuugggagucagacugcgagugauaa
gauccguagucgaaagggaaacagcccagaccgccagcuaaggucccaaa
guauacguuaaguggaaaaggauguggaguugcuuagacaaccaggaugu
uggcuuagaagcagccaccauuuaaagagugcguaauagcucacuggucg
agugacucugcgccgaaaauguaccggggcuaaacguaucaccgaagcug
cggacuguucgaacagugguaggagagcguucuaagggcugugaagccag
accggaaggacugguggagcgcuuagaagugagaaugccgguaugaguag
cgaaagaggggugagaauccccuccaccgaaugccuaagguuuccugagg
aaggcucguccgcucaggguuagucgggaccuaagccgaggccgaaaggc
guaggcgauggacaacagguugauauuccuguaccaccuccucaccauuu
gagcaauggggggacgcaggaggauaggguaagcgcgguauuggauaucc
gcguccaagcaguuaggcugggaaauaggcaaauccguuucccaaaggcu
gagcugugauggcgagcgcgaaguuccugauuccacacugccaagaaaag
ccucuagcgaggugagaggugcccguaccgcaaaccgacacagguaggcg
aggagagaauccuaaggugaucgagagaacucucguuaaggaacucggca
aaaugaccccguaacuucgggagaaggggugcucuguuagggugcaagcc
cgagagagccgcagugaauaggcccaggcgacuguuuagcaaaaacacag
gucucugcgaagccguaaggcgaaguauaggggcugacgccugcccggug
cuggaagguuaagaggagcgcuuagcguaagcgaaggugcgaauugaagc
cccaguaaacggcggccgucgguccuaagguagcgaaauuccuugucggg
uaaguuccgacccgcacgaaaggcgcaacgaucugggcacugucucaacg
agagacucggugaaauuauaguaccugugaagaugcagguuacccgcgac
aggacggaaagaccccguggagcuuuacugcagccugauauugaauguug
guuugaccuucuaacccgccgcccuuaucgggcggggagacagugucagg
ugggcaguuugacuggggcggucgccuccuaaaagguaacggaggcgccc
aaagguucccucagaaugguuggaaaucauucgcagaguguaaaggcaca
agggagcuugacugcgagaccuacaagucgagcagggacgaaagucgggc
uuagugauccggugguuccgcauggaagggccaucgcucaacggauaaaa
gcuaccccggggauaacaggcuuaucucccccaagaguccacaucgacgg
ggagguuuggcaccucgaugucggcucaucgcauccuggggcuguagucg
gucccaaggguugggcuguucgcccauuaaagcgguacgcgagcuggguu
cagaacgucgugagacaguucggucccuauccgucgcgggcgcaggaaau
uugagaggagcuguccuuaguacgagaggaccgggauggacgcaccgcug
guguaccaguuguucugccaagggcaucgcuggguagcuaugugcggacg
ggauaagugcugaaagcaucuaagcaugaagccccccucaagaugagauu
ucccauuccgcaaggaaguaagaucccugaaagaugaucagguugauagg
ucugagguggaaguguggcgacacauggagcugacagauacuaaucgauc
gaggacuuaacc

<<<<<<<......<<<<<<<<<<<....{[[..[[<<<<<<<[[<<....
..<<<<<<..[..((.>>>.....<<<<<<<..<<.))..]...>>....
.>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<
<<<<<<<<.........>>>>>>>.>>.>>....<<<<<<.....<<...
.>>......>>>>>.>.........<<....>>...<<<<......<<..
...>>.....>>>>...(((....<<<<<<<<....>>>>>>>>......
<<<<<<<<<.....<<<<.......<<<<<<<<<<.<<<.....<<<<..
....>>>>.((......<<<.....))>>>...>>>...>>>>>>>>>>>
>>>.>>>>>..>>>>.....<<<<<<<<.........>>>>>>>>.....
.<<<<<..<<<<>>>>>..)))......]].>>>>>>>.]].........
......<<....>>....]].........<<<<....>>>>.........
....}....>>>>>>>>>>>.......<<<<..<<<<......>>>>..>
>>>...<.<........>.>.<<<<<<....<<<<<<<.<<<<<..<<<.
..<<<.....>>>...>>>...<<....>>..<<<<.....>>>>.....
.>>>>>.>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<..
..<<<<<<......>>>>>>.....>>>...<<<.<<<<<........>>
>>>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>...>.
.>.>>..<<<<<<....<<<<<....>>>>><<<<<<<<<...<<<<<<<
<<.....<<<...<<<<<<<<...<<.<<<..>>>..>>...>>>>>>>>
..>>.>.....>>>>.>>>>>..>>>>>>>.>>..<<<<<<<.<<.....
.>>.>>>>>>>....<<<......>>>...<<<<.<<<...<<<(((...
..<<<<<<...........<<..<<<<<<<<<<.......<<<<<.<<..
.<<<<.......>>>>...>>.........<<......>>...>>>>>..
.>>>>>>>>>>.>>...........))).....>>>>>>.>>>...>>>>
>>><<<<<<..>>>>>>....>>>>>><.<<<<<<<.<<......<<<<<
.<<....>>.>>>>>...>>.>>>>>>>..>.....>>>>>><......<
<....<<<<<<.......>>>>>><<<<....<<<<<.<<...<<<<<<.
..<<.....>>.>>>>>>....((<<<<.<<...<<<...<<<....>>>
...>>>...>>....<<<<........>>>>.))<<<<..<<<<<<....
..<<...<<<<....<<<<..<<<..<<<.<<<<<<<<...(((....>>
>>>><<..<<<<<..<<<<<<<<<<<.<<.....>>>>>>>>>...>>>>
..>>>>>...>><.........>>>>>>...>>>...>>>>.........
>>>>..>>..>>>>>>.>>>>>>>>..>>.....<<......>>>>>>>.
<<<<.....>>>>..>>>>..<<<<....<<<<<<<<.<<..........
.....<<<<<...............>>>>><<<<<....<<<<....>>>
>...>>>>>............<<<<<<<<<..<<<.........>>>..<
<<<<<<.....<<<....>>>.......>>>>>>>..<<<<.<<<..<<<
<<<<..<<<...<<...<<<<<<.<<....>>..>>>>>>...>>...>>
>>>>>>..>>>>><<<<<.>>>>>........<...........<<<<<<
.....>>>>>>.....>....>>>>...>>.>>>>>>>.....>>.>>>>
>>>>.>>>>>>.....>.....<<<<<........>>>>>..<<<<<<<<
.(((.[[......<<<<.[[<<<<..<<<<<.<<<<<<<<<.<<<.<<<.
....>>>.>>>....<<.<<<<<<.....>>>>>>>>.....>>>>>>>>
>..>>>>>..<<<<.....>>>><<<<<<...........>>>>>>..<<
...<<<<<<<...<<<<<<.......>>>>>>.....<<.[.(((>>...
.>>>>>>>...<<<<..<<<......>>>..>>>>.<<<....>>>.>>)
))]......<<<<<<<.((..>>>>..))>>>>>>>.............>
>>>..]]>>>>.......<<<<.<.<<<<<<...<<.......>>...>>
>>>>...>>>>.>.......<.<<<.<<<<<<..<<<<<<<<<......>
>>>>>..>>>.<<<<<.....>>>>>.....>>>>>..>...>>>..>..
..<<<<<<<....>>>.>>>>....]]..)))>>>>>>>>....<<<<<<
<...<<<<..<<<<<<<...............>>>>>>>.<<<<<...<<
<<...<<<<<<<<<<<.)))>>>>>>..>>>>>...>>>>.>>>>>...<
<<.<..<<<<<...........>>>>>...>>>>.>>>>.......>>>>
>>>..<<<<....>>>>....<<<<<<<<<.......>>>>>....<<<<
<<<<.<<<....<<<<<....>>>>>...>>>..>>>>>.>>>...>>>>
....>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7as9 Structural Basis for Bacterial Ribosome-Associated Quality Control by RqcH and RqcP.
• Resolution: 3.5 Å
• Binding residue (original residue number in PDB): P4 K5 R6 K8 Y9 R10 R11 E12 H13 R14 G15 K16 M17 R18 K22 G23 G24 H28 F29 W41 R45 Q46 R56 W65 F69 K77 E80 V81 R82 M83 G84 S85 G86 K87 R119 L120 H123 K124 L125 P126 K128
• Binding residue (residue number reindexed from 1): P4 K5 R6 K8 Y9 R10 R11 E12 H13 R14 G15 K16 M17 R18 K22 G23 G24 H28 F29 W41 R45 Q46 R56 W65 F69 K77 E80 V81 R82 M83 G84 S85 G86 K87 R119 L120 H123 K124 L125 P126 K128

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7as9, PDBe:7as9, PDBj:7as9
• PDBsum: 7as9
• PubMed: 33259810
• UniProt: P14577|RL16_BACSU Large ribosomal subunit protein uL16 (Gene Name=rplP)