Structure of PDB 6wru Chain Q Binding Site BS01

Receptor Information

>6wru Chain Q (length=64) Species: 1280 (Staphylococcus aureus)

PKMKTHRGAAKRVKRTASGQLKRSRAFTSHLFANKSTKQKRQLRKARLVS
KSDMKRVKQLLAYK

Ligand information

>6wru Chain 1 (length=2665)

uaaguuauuaagggcgcacgguggaugccuuggcacuagaagccgaugaa
ggacguuacuaacgacgauaugcuuuggggagcuguaaguaagcuuugau
ccagagauuuccgaauggggaaacccagcaugaguuaugucauguuaucg
auaugugaauacauagcauaucagaaggcacacccggagaacugaaacau
cuuaguacccggaggaagagaaagaaaauucgauucccuuaguagcggcg
agcgaaacgggaagagcccaaaccaacaagcuugcuuguugggguuguag
gacacucuaguuacaaaggacgacauuagacgaaucaucuggaaagauga
aucaaagaagguaauaauccuguagucgaaaauguugucucucuugagug
gauccugaguacgacggagcacgugaaauuccgucggaaucugggaggac
caucuccuaaggcuaaauacucucuagugaccgauagugaaccaguaccg
ugagggaaaggugaaaagcaccccggaaggggagugaaauagaaccugaa
accgugugcuuacaaguagucagagcccguuaaugggugauggcgugccu
uuuguagaaugaaccggcgaguuacgauuugaugcaagguuaagcaguaa
auguggagccguagcgaaagcgagucugaauagggcguuuaguauuuggu
cguagacccgaaaccaggugaucuacccuuggucagguugaaguucaggu
aacacugaauggaggaccgaaccgacuuacguugaaaagugagcggauga
acugaggguagcggagaaauuccaaucgaaccuggagauagcugguucuc
uccgaaauagcuuuagggcuagccucaagugaugauuauuggagguagag
cacuguuuggacucagacaaacuccgaaugccaauuaauuuaacuuggga
gucagaacaugggugauaagguccguguucgaaagggaaacagcccagac
caccagcuaaggucccaaaauauauguuaaguggaaaaggauguggcguu
gccgugacacugcgccgaaaauguaccggggcuaaacauauuaccgaagc
uguggauuguccuuuggacaaugguaggagagcguucuaagggcguugaa
gcaugaucguaaggacauguggagcgcuuagaagugagaaugccggugug
aguagcgaaagacgggugagaaucccguccaccgauugacuaagguuucc
agaggaaggcucguccgcucuggguuagucggguccuaagcugaggccga
caggcguaggcgauggauaacagguugauauuccuguaccaccuauaucg
auggggggacgcaguaggauaggcgaagcgugcgauuggauugcacgucu
aagcaguaaggauccgguacgcugagcugugauggggagaagacauugug
ucuucgagucguugauuucacacugccgagaaaagccucuagggugcccg
uaccgcaaaccgacacagguagucaagaugagaauucuaaggugagcgag
cgaacucucguuaaggaacucggcaaaaugaccccguaacuucgggagaa
ggggugcucuuuagggucccagaagagccgcagugaauaggcccaagcga
cuguuuaucaaaaacacaggucucugcuaaaccguaaggugauguauagg
ggcugacgccugcccagugcuggaagguuaagaggagugguuagcuucug
cgaagcuacgaaucgaagccccaguaaacggcggccguaacuauaacggu
ccuaagguagcgaaauuccuugucggguaaguuccgacccgcacgaaagg
cguaacgauuugggcacugucucaacgagagacucggugaaaucauagua
ccugugaagaugcagguuacccgcgacaggacggaaagaccccguggagc
uuuacuguagccugauauugaaaugcuuucuaacccgcaccacuuaucgu
ggugggagacagugucaggcgggcaguuugacuggggcggucgccuccua
aaagguaacggaggcgcucaaagguucccucagaaauucauagaguguaa
aggcauaagggagcuugacugcgagaccuacaagucgagcagggucgaaa
gacggacuuagugauccggugguuccgcauggaagggccaucgcucaacg
gauaaaagcuaccccggggauaacaggcuuaucucccccaagaguucaca
ucgacggggagguuuggcaccucgaugucggcucaucgcauccuggggcu
guagucggucccaaggguugggcuguucgcccauuaaagcgguacgcgag
cuggguucagaacgucgugagacaguucggucccuauccgucgugggcgu
aggaaauuugagaggagcuguccuuaguacgagaggaccgggauggacau
accucugguguaccaguugucgugccaacggcauagcuggguagcuaugu
guggacgggauaagugcugaaagcaucuaagcaugaagccccccucaaga
ugagauuucccaacuucgguuauaagaucccucaaagaugaugagguuaa
uagguucgagguggaagcauggugacauguggagcugacgaauacuaauc
gaucgaagacuuaau

<<<<<......<<<<<<<<<<<....{[[..[[<<<<<<<[[<<......
<<<<<<.[...((.>>>.....<<<<<<<..<<<))...].>>>.....>
>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<<
<<<<<<.........>>>>>>>.>>.>>....<<<<<......<......
>.......>>>>.>.........<<....>>..<<<<<......<<....
.>>.....>>>>>..(((....<<<<<<........>>>>>>......<<
<<<<<<<...........<.<<<<<<<.<<<...<<<<<......>>>>>
((......<<<.....))>>>...>>>...>>>>>>>..>.....>>>>>
..>>>>.....<<<<<<<<.........>>>>>>>>......<<<<<..<
<<<>>>>>..)))......]].>>>>>>>.]]...............<<.
...>>....]].........<<<<....>>>>.............}....
>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>>>...<.<
........>.>.<<<<<<....<<<<<<<.<<<<<..<<<...<<<....
.>>>...>>>...<<....>>..<<<<.....>>>>.....>>>>>.>>>
>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<....<<<<<<..
....>>>>>>.....>>>...<<<.<<<<<........>>>>>>>>....
>>>>>>>>>...<<<.....>>>......>>>>>>...>..>.>>..<<<
<<<....<<<<<....>>>>><<<<<<<<<...<<<<<<<<<.....<<<
...<<<<<<<..>>>>>>>..>>.>.....>>>>.>>>>>..>>>>>>>.
>>..<<<<<<<<<<......>>>>>>>>>>....<<<......>>>...<
<<<.<<<...<<<(((.....<<<<<<...........<<..<<<<.<<<
<...>>>>.>>>>.>>...........))).....>>>>>>.>>>...>>
>>>>><<<<<<.....>>>>>>....>>>>>><.<<<<<<<.<<......
<<<<<.<<....>>.>>>>>...>>.>>>>>>>..>.....>>>>>><..
....<<....<<<<<<.......>>>>>><<<<....<<<<<.<<...<<
<<<<...<<.....>>.>>>>>>....((<<<<.<<...<<<...<<<..
..>>>...>>>...>>...<<<<<........>>>>>))<<<<.......
..<<<<....<<<<...<<...<....<.<<<.....{...>>>.>.<..
..<<<<...<<..........>>...>>>>.....>.....<<.......
..>>........>....>>...>>>>.........>>>.>..>>>>>>>>
..>>.....<<......>>>>>>>.<<<.......>>>..>>>>..<<<<
....<<<<<<<<.<<.........<.....<<<<<....<....>.....
>>>>><<<<<<<.<<<.>>>.>>>>>>>............<<<<<<<<<.
.<<<.........>>>..<<<<<<<.....<<<....>>>.......>>>
>>>>.<<<<<.<<<..<<<<<<<..<<<<..<<...<<<<<<.<<....>
>..>>>>>>...>>..>>>>>>>>>..>>>>><<<<<<.......>>>>>
>........<...........<<<<<.......>>>>>.....>....>>
>>>..>>.>>>>>>>..>..>>.>>>>>>>>.>>>>>>.....>.....<
<<<<........>>>>>..<<<<<<<<.(((.[[......<<<<.[[<<<
<..<<<<<.<<<<<<<<<.<<<<...>>>>....<<.<<<<<<.....>>
>>>>>>.....>>>>>>>>>..>>>>>..<<<<.....>>>><<<<<<..
.........>>>>>>..<<...<<<<<<<...<<<.>>>.....<<.[.(
((>>....>>>>>>>...<<<<..<<<......>>>..>>>>.<<<....
>>>.>>)))]......<<<<<<<.((..>>>>..))>>>>>>>.......
......>>>>..]]>>>>.......<<<<.<.<<<<<<...<<.......
>>...>>>>>>...>>>>.>.......<.<<<.<<<<<<..<<<<<<<<<
......>>>>>>..>>>.<<<<<.....>>>>>.....>>>>>..>...>
>>..>....<<<<<<<....>>>.>>>>....]]..)))>>>>>>>>...
.<<<<<<<...<<<<..<<<<<<<...............>>>>>>>.<<<
<<...<<<<...<<<<<<<<<<<.}..>>>>>>..>>>>>...>>>>.>>
>>>...<<<.<..<<<<<...........>>>>>...>>>>.>>>>....
...>>>>>>>.<<<....>>>....<<<<<<<<<.......>>>>>....
<<<<<<<<.<<<....<<<<<....>>>>>...>>>..>>>>>.>>>...
>>>>....>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6wru Characterization of the Core Ribosomal Binding Region for the Oxazolidone Family of Antibiotics Using Cryo-EM.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): P2 K3 M4 K5 T6 H7 R8 K12 T17 A18 S19 R24 R26 A27 F28 S30 H31 F33 A34 N35 Q40 R45 S53 R57 Y64
• Binding residue (residue number reindexed from 1): P1 K2 M3 K4 T5 H6 R7 K11 T16 A17 S18 R23 R25 A26 F27 S29 H30 F32 A33 N34 Q39 R44 S52 R56 Y63

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6wru, PDBe:6wru, PDBj:6wru
• PDBsum: 6wru
• PubMed: 32566908
• UniProt: Q2FXQ0|RL35_STAA8 Large ribosomal subunit protein bL35 (Gene Name=rpmI)