Structure of PDB 4wfa Chain Q Binding Site BS01

Receptor Information

>4wfa Chain Q (length=88) Species: 93061 (Staphylococcus aureus subsp. aureus NCTC 8325)

EARDILKRPVITEKSSEAMAEDKYTFDVDTRVNKTQVKMAVEEIFNVKVA
SVNIMNYKPKKKRMGRYQGYTNKRRKAIVTLKEGSIDL

Ligand information

>4wfa Chain X (length=2711)

gauuaaguuauuaagggcgcacgguggaugccuuggcacuagaagccaau
gaaggacguuacuaacgacgauaugcuuuggggagcuguaaguaagcuuu
gauccagagauuuccgaauggggaaacccagcaugaguuaugucauguua
ucgauauguauacauagcauaucagaaggcacacccggagaacugaaaca
ucuuaguacccggaggaagagaaagaaaauucgauucccuuaguagcggc
gagcgaaacgggaagagcccaaaccaacaagcuugcuuguugggguugag
gacacucuaugaguuacaaaggacgacauuagacgaaucaucuggaaaga
ugaaucaaagaagguaauaauccuguagucgaaaauguugucucucuuga
guggauccugaguacgacggagcacgugaaauuccgucggaaucugggag
gaccaucuccuaaggcuaaauacucucuagugaccgauagugaaccagua
ccgugagggaaaggugaaaagcaccccggaaggggagugaaauagaaccu
gaaaccgugugcuuacaaguagucagagcccguuaaugggugauggcgug
ccuuuuguagaaugaaccggcgaguuacgauuugaugcaagguuaagcag
uaaauguggagccguagcgaaagcgagucugaauagggcguuuaguauuu
ggucguagacccgaaaccaggugaucuacccuuggucagguugaaguuca
ggcugaauggaggaccgaaccgacuuacguugaaaagugagcggaugaac
ugaggguagcggagaaauuccaaucgaaccuggagauagcugguucucuc
cgaaauagcuuuagggcuagccucaagugaugauuauuggagguagagca
cuguuuggacgaggggguuaccgaauucagacaaacuccgaaugccaauu
aauuuaacuugggagucagaacaugggugauaagguccguguucgaaagg
gaaacagcccagaccaccagcuaaggucccaaaauauauguuaaguggaa
aaggauguggcguugcccagacaacuagcucacuagucgagugacacugc
gccgaaaauguaccggggcuaaacauauuaccgaagcuguggauuguccu
uggacaaugguaggagagcguucuaagggcguugaagcaugaucguaagg
acauguggagcgcuuagaagugagaaugccggugugaguagcgaaagacg
ggugagaaucccguccaccgauugacuaagguuuccagaggaaggcucgu
ccgcucuggguuagucggguccuaagcugaggccgacaggcguaggcgau
ggauaacagguugauauuccuguaccaccuauaaucguaucgaugggggg
acgcaguaggauaggcgagcgugcgauuggaugcacgucuaagcaguagg
cugaguaaaauccgguacucggcugagcugugauggggagaagagagucg
uugauuucacacugccgagaaaagccucuagauagaauaggugcccguac
cgcaaaccgacacagguagucaagaugagaauucuaaggugagcgagcga
acucucguuaaggaacucggcaaaaugaccccguaacuucgggagaaggg
gugcucuuuaggguuaacgcccagaagagccgcagugaauaggcccaagc
gacuguuuaucaaaaacacaggucucugcuaaaccguaaggugauguaua
ggggcugacgccugcccgugcuggagguuaagaggagugguuagcugcga
agcuacgaaucgaagccccaguaaacggcggccuaagguagcgaaauucc
uugucggguaaguuccgacccgcacgaaaggcguaacgauuugggcacug
ucucaacgagagacucggugaaaucauaguaccugugaagaugcagguua
cccgcgacaggacggaaagaccccguggagcuuuacuguagccugauauu
gaaauucuggcuuucuaacccgcaccacuuaucguggugggagacagugu
caagcgggcaguuugacuggggcggucgccuccuaaaagguaacggaggc
gcucaaagguucccucagaaugguuggaaaucauucauagaguguaaagg
cauaagggagcuugacugcgagaccuacaagucgagcagggucgaaagac
ggacuuagugauccggugguuccgcauggaagggccaucgcucaacggau
aaaagcuaccccggggauaacaggcuuaucucccccaagaguucacaucg
acggggagguuuggcaccucgaugucggcucaucgcauccuggggcugua
gucggucccaaggguugggcuguucgcccauuaaagcgguacgcgagcug
gguucagaacgucgugagacaguucggucccuauccgucgugggcguagg
aaauuugagaggagcuguccuuaguacgagaggaccgggauggacauacc
ucugguguaccaguugucgugccaacggcauagcuggguagcuaugugug
gacgggauaagugcugaaagcaucuaagcaugaagccccccucaagauga
gauuucccaacuugguuauaagaucccucaaagaugaugagguuaauagg
uucgagguggaagcauggugacauguggagcugacgaauacuaaucgauc
gaagacuuaau

..<<<<<<......<<<<<<<<<<<.....[[..[[<<<<<<<[[<<...
...<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>...
..>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.
<<<<<<<<<.......>>>>>>>.>>.>>....<<<<<......<<....
>>.......>>>>.>.........<<....>>..<<<<<......<<...
..>>.....>>>>>..(((....<<<<<<<<....>>>>>>>>.....<<
<<<<<<<...<..........<<<<<<<<<.<<<...<<<<<......>>
>>>((......<<<.....))>>>...>>>...>>>>>>>>.>...>.>>
>>>..>>>>.....<<<<<<<<.........>>>>>>>>......<<<<<
..<<<<>>>>>..)))......]].>>>>>>>.]]...............
<<....>>....]].........<<<<....>>>>...............
...>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>>>...
<.<........>.>.<<<<<<....<<<<<<<.<<<<<..<<<...<<..
.....>>...>>>...<<....>>..<<<<.....>>>>.....>>>>>.
>>>>>>>..<<.<...<<<<<<.....<<<<<<<<<..<<<....<<<<.
....>>>>.....>>>...<<<.<<<<<........>>>>>>>>....>>
>>>>>>>...<<<.....>>>......>>>>>>......>.>>..<<<<<
<....<<<<<....>>>>><<<<<<<<<...<<<<<<<<<.....<<<..
.<<<<<<<<...<<......>>...>>>>>>>>..>>.>.....>>>>.>
>>>>..>>>>>>>.>>..<<<<<<<<<<......>>>>>>>>>>....<<
<......>>>...<<<<.<<<...<<<(((.....<<<<<<.........
..<<..<<<<.<<<<........<<<<......>>>>....>>>>.>>>>
.>>...........))).....>>>>>>.>>>...>>>>>>><<<<<<<.
.>>>>>>>....>>>>>>..<<<<<<<.<<......<<<<<.<<....>>
.>>>>>...>>.>>>>>>>........>>>>>><......<<....<<<<
<<.......>>>>>><<<<....<<<<<.<<...<<<<<<...<<.....
>>.>>>>>>....((<<<<.<<...<<<...<<<....>>>...>>>...
>>...<<<<<........>>>>>))<<<<<<<......<<<...<<<<..
..<<<<...<<...<...<.<<<...{.{..>>>.>.<....<<<<<.<<
<.<<.<..........>.>>.>>>..>>>>>.....>.............
.>....>>...>>>>.........>>>.>.>>>...>>>>>>>>>>>..>
>.....<<......>>>>>>>.<<<<....>.>>>..>>>>..<<<<...
.<<<<<<<<.<<...............<<<<<.....<..>......>>>
>><<<<<<<.<<<<....>>>>.>>>>>>>............<<<<<<<<
<..<<<.........>>>..<<<<<<<.....<<<....>>>.......>
>>>>>>.<<<<<..<<.<<<<<<<.<<<...<<...<<<<<..<<.>>..
.>>>>>...>>...>>>>>>>>..>>>>............<.........
..<<<<<<.....>>>>>>.....>....>>>>>..>>.>>>>>>>....
.>>.>>>>>>>>.>>>>>>.....>.....<<<<<........>>>>>..
<<<<<<<<.(((.[[......<<<<.[[<<<<..<<<<..<<.<<<<<<.
<<<<.......>>>>....<<.<<<<<<.....>>>>>>>>.....>>>>
>>.>>...>>>>..<<<<.....>>>><<<<<<...........>>>>>>
..<<...<<<<<<<...<<<<<<.......>>>>>>.....<<.[.(((>
>....>>>>>>>...<<<<..<<<......>>>..>>>>.<<<....>>>
.>>)))]......<<<<<<<.((..>>>>..))>>>>>>>..........
...>>>>..]]>>>>.......<<<<.<.<<<<<<...<<.......>>.
..>>>>>>...>>>>.>.......<<<<<.<<<<<<..<<<<<<<<<...
...>>>>>>>..>>.<<<<<.....>>>>>.....>>>>>..>...>>>.
>>....<<<<<<<....>>>.>>>>....]]..)))>>>>>>>>....<<
<<<<<...<<<<..<<<<<<<...............>>>>>>>.<<<<<.
..<<<<...<<<<<<<<<<<.}.}>>>>>>..>>>>>...>>>>.>>>>>
...<<<....<<<<<...........>>>>>....>>>.>>>>.......
>>>>>>>.<<<<.>>>>....<<<<<<<<<.......>>>>>....<<<<
<<<<.<<<....<<<<<....>>>>>...>>>..>>>>>.>>>...>>>>
....>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4wfa Structural insights into species-specific features of the ribosome from the pathogen Staphylococcus aureus.
• Resolution: 3.392 Å
• Binding residue (original residue number in PDB): T13 N34 K35 T36 N54 I55 M56 Y58 K59 M65 Y68 G70 Y71 N73 K74 R76 I79
• Binding residue (residue number reindexed from 1): T12 N33 K34 T35 N53 I54 M55 Y57 K58 M64 Y67 G69 Y70 N72 K73 R75 I78

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:4wfa, PDBe:4wfa, PDBj:4wfa
• PDBsum: 4wfa
• PubMed: 26464510
• UniProt: Q2FW08|RL23_STAA8 Large ribosomal subunit protein uL23 (Gene Name=rplW)