Structure of PDB 4d5l Chain Q Binding Site BS01

Receptor Information

>4d5l Chain Q (length=139) Species: 9986 (Oryctolagus cuniculus)

QSVQVFGRKKTATAVAHCKRGNGLIKVNGRPLEMIEPRTLQYKLLEPVLL
LGKERFAGVDIRVRVKGGGHVAQIYAIRQSISKALVAYYQKYVDEASKKE
IKDILIQYDRTLLVADPRRCESKKFGGPGARARYQKSYR

Ligand information

>4d5l Chain 1 (length=1742)

uaccugguugauccugccaguagcauaugcuugucucaaagauuaagcca
ugcaugucuaaguacgcacggccgguacagugaaacugcgaauggcucau
uaaaucaguuaugguuccuuuggucgcucgcuccucucccacuuggauaa
cugugguaauucuagagcuaauacaugccgacgggcgcugacccccuucg
cgggggggaugcgugcauuuaucagaucaaaaccaacccguagauaaccu
cgggccgaucgcacgccccccguggcggcgacgacccauucgaacgucug
cccuaucaacuuucgaugguagucgccgugccuaccauggugaccacggg
ugacggggaaucaggguucgauuccggagagggagccugagaaacggcua
ccacauccaaggaaggcagcaggcgcgcaaauuacccacucccgacccgg
ggagguagugacgaaaaauaacaauacaggacucuuucgaggcccuguaa
uuggaaugaguccacuuuaaauccuuuaacgaggauccauuggagggcaa
gucuggugccagcagccgcgguaauuccagcuccaauagcguauauuaaa
guugcugcaguuaaaaagcucguaguuggaucuugggagcgcgccccuug
ccucucggcgcgcccgaagcguuuacuuugaaaaaauuagaguguucaaa
gcaggcccgagccgccuggauaccgcagcuaggaauaauggaauaggacc
gcgguucuauuuuguugguuuucggaacugaggccaugauuaagagggac
ggccgggggcauucguauugcgccgcuagaggugaaauucuuggaccggc
gcaagacggaccagagcgaaagcauuugccaagaauguuuucauuaauca
agaacgaaagucggagguucgaagacgaucagauaccgucguaguuccga
ccauaaacgaugccgaccggcgaugcggcggcguuauucccaugacccgc
cgggcagcuuccgggaaaccaaagucuuuggguuccggggggaguauggu
ugcaaagcugaaacuuaaaggaauugacggaagggcaccaccaggagugg
agccugcggcuuaauuugacucaacacgggaaaccucacccggcccggac
acggacaggauugacagauugauagcucuuucucgauuccguggguggug
gugcauggccguucuuaguugguggagcgauuugucugguuaauuccgau
aacgaacgagacucuggcaugcuaacuaguuacgcgacccccgagcgguc
ggcgucccccaacuucuuagagggacaaguggcguucagccacccgagau
ugagcaauaacaggucugugaugcccuuagauguccggggcugcacgcgc
gcuacacugacuggcucagcgugugccuacccuacgccggcaggcgcggg
uaacccguugaaccccauucgugauggggaucggggauugcaauuauucc
ccaugaacgaggaauucccaguaagugcgggucauaagcuugcguugauu
aagucccugcccuuuguacacaccgcccgucgcuacuaccgauuggaugg
uuuagugaggcccucggaucggccccgccggggucggcccacggccucgg
cggagcgcugagaagacggucgaacuugacuaucuagaggaaguaaaagu
cguaacaagguuuccguaggugaaccugcggaaggaucauua

...<<<<..{.[[[[.>>>><<<.<<<<<<...<.<<..<......<<<.
<<<..<<...<<<.....................>>>>>......<<...
.....<<<......<<....<<<......................<....
<<.<<.......>>.>>......>...........<<<...<<<<<....
..>>>>>...>>>.....<<<<<........((((((.....>>>>>...
........<<<<..<<<......>>>.>>>>....>>>...>>....>>>
.<<<....<<<....<<<<<<<.........>>>>>>>>>>......>>>
...<<<<<<<<....>>>>...>>>>.>>.<<.<<<..........>>>.
>>.<.<<....>>.>...>>>>>>.........<<<....<<<<...>>>
>..>>>..>...>>.>.....<<<<<<<<<.<<<....>>>..>>>>>.>
>>>......<<..<...........>..>>.........<<<<<((....
..<<<<.....<<..))>>.......>>>>.>>>>>..>>>>>>.>>>..
.......<.<[[.....<.<<...<<<.<<....<<<.<<<>>>>>>...
....<<<<.<..>>>>>.....<<<<<..<.......<<...<.......
>..<<<.......>>>....>>......>..>>..>>>..........<<
.<<<<<<<.....))))))....>>>>>>>.>>......>>....<<<<<
<......<<<<..<<..<<<<<<<....<<<......>>>.......>>>
>>>>..>>.......<<....>>...>>>>.....>>>.>>>...>>>..
.>>.>....<<<<<<<...<...<<<<.........>>>>...>>>>>>>
>..........<<<.<<.<<<....<<<<<<.<<<<......>>>>>>>>
>>....>>>...<<..]]>>...>>.....>>>.>.>.<<<......<<<
<....>>>>....>>>..]]]].}<<<<<.<<<<<<<..<<..<<<<<..
<<<.<<<<........<<........>>..........<<<<<.<....<
<<<<........<<.<<<........>>>.>>......>>>>>...<<.<
<<..<<.<<<<<<....<<<.<<<<<....>>>...<<<......>>>..
.>>.>>>....<<<...<...<<<...<.<<....<<...<<<<....>>
>>..>>....>>.>...>>>..>.....<<<<<.....>>>>>.......
.>>>....>>>.>>>.....>>>>>>>.....>.>>>>>......>>>>.
>>>.....<<.<<<................<<<.<<<<....>>>>.>>>
..............>>>.>>......<.....<<<<<..........>>>
>>.....>.....>>>>>.....<<<<<<<<.......>>>>>>>>....
..>>...>>>>>>>>>>.>>.......<<.<..<<<<.<<....<<<<<<
<<.<<<..<<<<.<<...<<<<<.<<<<<<<<<<<<.....>>>>>>>>>
>>>...>>>>>...>>.>>>>..>>>.>>>>>>>>...>>.>>>>...>.
>>.........<<<<<<<<<....>>>>>>>>>.........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4d5l Cryo-Em of Ribosomal 80S Complexes with Termination Factors Reveals the Translocated Cricket Paralysis Virus Ires.
• Resolution: 9.0 Å
• Binding residue (original residue number in PDB): Q11 F13 K17 T18 V22 H24 L31 K33 G36 R37 E43 R69 R71 K73 G74 G76 H77 V78 A79 Q80 R125 R126 C127 E128 S129 K130 K131 F132 A137 R138 A139 R140 Y141 Q142 K143 Y145 R146
• Binding residue (residue number reindexed from 1): Q4 F6 K10 T11 V15 H17 L24 K26 G29 R30 E36 R62 R64 K66 G67 G69 H70 V71 A72 Q73 R118 R119 C120 E121 S122 K123 K124 F125 A130 R131 A132 R133 Y134 Q135 K136 Y138 R139

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006364 rRNA processing
• GO:0006412 translation
• GO:0042274 ribosomal small subunit biogenesis
• GO:1990830 cellular response to leukemia inhibitory factor

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022626 cytosolic ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:0032040 small-subunit processome
• GO:0045202 synapse
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:4d5l, PDBe:4d5l, PDBj:4d5l
• PDBsum: 4d5l
• PubMed: 25601755
• UniProt: G1SGX4|RS16_RABIT Small ribosomal subunit protein uS9 (Gene Name=RPS16)