Structure of PDB 7oyb Chain P1 Binding Site BS01

Receptor Information

>7oyb Chain P1 (length=152) Species: 7955 (Danio rerio)

VRYSLDPENPTKSCKSRGSNLRVHFKNTRETAQAIKGMHIRKANKYLKDV
IVKHQCVPFRRYNGGVGRCAQAKQHDWTQGRWPKKSAEFLLHMLKNAESN
AELKGLDVDSLVIEHIQVNKAPKMRRRTYRAHGRINPYMSSPCHIEMILT
EK

Ligand information

>7oyb Chain 51 (length=3263)

cacgaccucagcucagacgagaagacccgcugaauuuaagcauauuacua
agcggaggaaaagaaaccaaccgggauucccccaguagcggcgagcgaag
agggaaaaguccagcgccgaauccccgccccagggcgagggaccuguggc
guacggagggccgccucucucggcgcgggggccaaaguccuucugaugga
ggcuuagcccgcggacggugugaggccggugucggcccccgccccgccgg
ggugcgguuccucccggagucggguuguuugggaaugcagcccaaagcgg
gugguaaacuccaucuaaggcuaaauaccggcacgagaccgauagcggac
aaguaccgugagggaaaguugaaaagaacuuugaagagagaguucaacag
ggcgugaaaccguuaagagguaaacggguggggaccgcccgcccggugga
uucagcccggcggggcggggucggcccguccgguggcucccuucgcgggg
uugacgcccgggcgaaggcucggccgccgccgggugcauuuccgccgcgg
uggagcgccgcgaccggcuccgguucggcuuggaagggucagggggcgaa
gguuuuacagcgcccucccgccccgacgccgcuugcucuccggggccgcg
ggcccucucugcccucccucugcggggacggggucccccgcccccggcgu
ggcgcgacagggguggacuguccucaguccgcugcgccgcgccgcgggcg
ggggacccacguucgggcgcccgagguccgcggcgacgccggccucccac
ccgacccgucuugaaacacggaccaaggaguccaacgcgcgcgcgaguca
gaggguggcucgcgagccccugcggcgcaaugaaggugagggugggaucc
ccccgccccggcggggggcgcaccaccggcccgccucgcgacccuccggg
gggcagguggaguuggagcgcgcgcgauggcacccgaaagauggugaacu
augccugggcagggcgaagccaggggaaacucugguggaggcccgccgcg
guccugacgugcaaaucggucguccgaccugggcauaggggcgaaagacu
aaucgaaccaucuaguagcugguucccuccgaaguuucccucaggauagc
uggcgcucgccgaucaagcaguuuuauccgguaaagccaaugacuagagg
caccuauucucaaacuuuaaauggguaagaggcccggcucgcuggcgcug
gagccgggcguggaaugcgacgcgccuagugggccauuuuugguaagcag
aacuggugcugcgggaugaaccgaacgccggguuaaggcgcccgaugccg
acgcucaucagaccccauaaaagguguugguugauauagacagcccgaau
caacuagcccugaaaauggauggcgcuggagcgucgggcccauacccggc
cgucgacggcagcugcaagccucgacgaguaggagggccgccgcgguggc
gccgaagcccagggcgcgggcccggguggagccgccgcgggcgcagaucu
uggugguaguagcaaauauucaaacgagagcuuugaaggccgaaguggag
aaggguuccaugugaacagcaguugaacaugggugagccgguccuaaggg
acgggccuacgccguucggaggggagggccccgcucgaccgaaagggagu
cggguccagauccccgagcccggagcggcggagacgggcggcccagugcg
gugacgcaaacgaacccggagaugccggcgggugccccgggaaagaguuc
ucuuuucuuugugaagggcagggcgcccuggaacggguucgccccgagag
aggggcccgcgcccuggaaagcgccgcgcugcggcguccggugagcucuc
gucggcccuugaaaauccgggggagaagguguaaaucucgcgccgggccg
uacccauauccgcagcaggucuccaaggugaacagccucuggcguguugg
aacaaggcagaguaagggaagucggcaagucagauccguaacuucgggau
aaggauuggcucuaagggcugagcagccagcuuagaacugucgcggacca
ggggaauccgacuguuuaauuaaaacaaagcaucgcgaaggcccucggcg
gguguugacgcgaugugauuucugcccagugcucugaaugucaaagugaa
gaaauucaacgaagcgcggguaaacggcggucuuaagguagccaaaugcc
ucgucaucuaauuagugacgcgcaugaauggaugaacgagauucccacug
ucccuaccugcuaucuagcgaaaccacagccaagggaacgggcuuggcag
aaucagcggggaaagaagacccuguugagcuugacucuagucuggcccug
ugaagccucacuuacccggugaggcggguucuggcgucaagcgccccgcg
gggcgcgacccgccccggggacagcgucagguggggaguuugacuggggc
gguacaccugucaaacgguaacgcagguguccuaaggcgagcucaggggg
gacagaaaccucccguagagcagaagggcaaaagcucgcuugaucuugau
uuucaguaugaguacggaccgcgaaagcggggccucacgauccuuccggc
uuuugggguuuuaagcgggaggugucagaaaaguuaccacagggauaacu
ggcuuguggcggccaagcguucauagcgacgucgcuuuuugauccuucga
ugucggcucuuccuaucauugugaagcagaauucaccaagcguuggauug
uucacccacuaacagggaacgugagcuggguuuagaccgucgugagacag
guuaguuuuacccuacugaugugagccguuguugcaauaguaaucccgcu
caguacgagaggaaccgcggguucagacauuugguucgugcgcuuggcug
aggagccacuggcgcgaagccaccaucugcgggauuaugacugaacgccu
cuaagucagaaucccgccuaaaagcaacgauagcgccgucggaucuccga
uaggccccggguaaccgggaccccggcgcggagagccauucgugacagga
gcggcuagaacgagcgccgcccccucuuccagugacgcaccgcauguuug
uggggaacccggugcuuaaaugacucguagacgaccugauucugggucgg
ggugucgugcguggcagagcagcgcugcgauccauugaaagucagcccuc
gauccaaguuuug

.........................<<<<<<.....<......>......
>>>>>.>.........<<....>>..<<<<<......<<.....>>....
.>>>>>..<<<..<<<<<...<<<<<<<<<..>>>>>.>>>>.....>>>
>>..<<<<<<<<<....<<<.<..<<<<<<<<<.....<<<<......>>
>>.....((......<<<.....))>>>.....>>>>>>>>..>..>.>>
>...>>>>.>>>>>.......<<<<<<<........>>>>>>>.....<<
<<<..((((((>>>>>..>>>.............................
.....<<....>>...............<<<......>>>..........
........................<<<<<<<<..<<<..<<<<.<<<<<<
....<<<<<<<<<.....<.<.<.<.<<<<<<...<<....<<<...>>>
.....>>.>>>>>>..>..>.>.>..>>>>>>>>>.....>>>>>>..>>
>>.....<...<.<.<.<<<.<<...<....<<..<<...<<<<.<<...
.........>>.>>>>....>>......>>.>.....>>..>>>.>.>..
......<.<<...<<<<.<..>.>>>>..<<<<<<..<<.<.<.<<<<<<
...<<<<......<<<<<<<....>>>>>>>.>.>>>.>>>>>>...>.>
.>>>>>>>.>.....>>.>.......>....>.......>>>...>>>>>
>>>..<<<<........>>>><<<<<<....<<<.<<<<<<<<<..<<<.
..<<<<.........>>>>....>>>...<<....>>...<<<<<...<<
<<<<<<....>>>>>>>>....>>>>><<<<<<<<<.....<........
>...>>>>>>.>>>...>>>>>>>>>.>>>..<<.<..<<<<<<<.....
<<<<<<<<<..<<<....<<<<<<<....>>>>>>>.....>>>...<<<
..<<.<<........>>>>.>>>....>>>>>>>>>...<<.......>>
......>>>>>>...>..>.>>..<<<<<<....<.<<<....>>>.><<
.<<<<<<<.........<<<.<<<<<<<<<...<<<....<<<..<.<<<
..>>>.>..>>>..>.>>...>>>>>>>...<<<<<<<<<..........
>>>>>>>>>....>>>>>>>.>>>>>..>>..<<<<.<<<<<.....>>>
>>.>>>>....<<<......>>>...<<<<<<<...<<<(((.......<
<<<<......<..........<<<.<<<<<<<<<<.......<.>...>>
>>>>>>>>>>>..........))).>....>>>>>..>>>...>>>>>>>
<<<<<<.<<<........>>>>>>>>>....>>>>>>.<.<<<<<<<<<<
......<<<<.<<<<....>>>>>>>>...>>>>>>>>>>..>.....>>
>>>><<.....<<.....<<<<<<.......>>>>>>.<<<.....<<<<
<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<<<...<<.
...<<<....>>>..........<.<...>.>......>>....>>...<
<<<<........>>>>>))<<<<....<<.<....<<<<.>>>>...<<<
....>>>...<...<<<<<<...<<<<<<<<<.<<<<<<<.<<<<<<..[
[[..>>>>>>....<<<<<.<<<<.<<<<.....<<<.....>>>.....
>>>>>>>>..>>>>>.....<<<<<<<...>>>>>>>>>>>.>.>>.>>>
>>>>>>.........>>>>>>...>..........>...>>.>>>>>>>>
..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>....<<<
<....<<<<<.<<<.<<.........<.....<<<<<....<....>...
..>>>>><<.<<<<<<<<<<<..>>>>>..>>>>>>.>>...........
.<<<<<<<<<..<<<.........>>>..<<<<<<<....<<<<.....>
>>>......>>>>>>>...<<..<<<..<<<<<<....<<<.....<<<<
....>>>>.....>>>.>>>>..>>>>>............<.........
..<<<<<.......>>>>>.....>.....>>....>>.>>>>>>>..>.
.>>.>>>>>>>>.>>>>>>....>>....<<<<<........>>>>>...
.<<<<<..<<<.<<......<<<<.<<<<<<..<<<<<.<<<<<<<...<
<<<....>>>>...<<<<<.....<<<.............<<<<<<<..>
>>>>>>...>>>..>>>>>......>>>>>>>..>>>>>...<<<.....
>>>..<<<<<<...........>>>>>>..<<...<<<<<<<...<<<<<
<.......>>>>>>.....<<.[.(((>>....>>>>>>>...<<<..<<
<<.......>>>>..>>>.<<<....>>>.>>)))].....<<<<<<<..
<<<..)))))).>>>>>>>>>>.........>>>>..>>>>>>.......
<<.<...<<<<<<...<<.......>>...>>>>>>....>.>.>.....
..<.<<<.<<<<<<..<.<.<<<<<......>>>>>...>.>.<<<....
.....>>>.....>>>>>..>...>>>..>....<<<<<<<....>>>.>
>>>....>>..>>>.>>>>>.......<<<<<<<<..<<<....<<<<<.
................>>>>>..<<<<....<<<<....<<<<<<<<<<]
]].>>>>>..>>>>>...>>>>...>>>><<<<<...<<<<<........
...>>>>>...>>>>>.>>>...>>>>>>>>.<<<<<...<<.<<<<<..
..<<..........<<<<<..>>>>>.........>>...<<<....<<.
<<<<<..........>>>>>.......>>.....>>>.............
.>>>>>.>>.>>>>>..<<<...<.............>....<<<<<<<<
<<..<.....<<<<....<<<<..>>>>...>>>>....>.....>>>>.
>>>>>>...>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7oyb A molecular network of conserved factors keeps ribosomes dormant in the egg.
• Resolution: 2.4 Å
• Binding residue (original residue number in PDB): V2 R3 Y4 L6 K16 S17 R18 S20 R23 H25 F26 K27 N28 R30 Q34 K37 K43 K46 K54 R62 Y63 N64 G65 G66 V67 G68 R69 C70 A71 Q72 K74 Q75 W78 T79 Q80 R82 W83 P84 K85 K86 K96 N97 S100 N101 Q118 V119 K121 M125 R127 T129 Y130 R131 A132 H133 G134 R135 I136 N137 P138 Y139 M140 S141
• Binding residue (residue number reindexed from 1): V1 R2 Y3 L5 K15 S16 R17 S19 R22 H24 F25 K26 N27 R29 Q33 K36 K42 K45 K53 R61 Y62 N63 G64 G65 V66 G67 R68 C69 A70 Q71 K73 Q74 W77 T78 Q79 R81 W82 P83 K84 K85 K95 N96 S99 N100 Q117 V118 K120 M124 R126 T128 Y129 R130 A131 H132 G133 R134 I135 N136 P137 Y138 M139 S140

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7oyb, PDBe:7oyb, PDBj:7oyb
• PDBsum: 7oyb
• PubMed: 36653451
• UniProt: Q7T1K0