Structure of PDB 8eui Chain P Binding Site BS01

Receptor Information

>8eui Chain P (length=173) Species: 4896 (Schizosaccharomyces pombe)

VRYSASPALETKCAKARGAYLRTHFKNSREVAFTINGMSLKKAFIFLDNV
KEHKQAVPFRRFNGGVGRTAQGKEFGVTQARWPVKSVKFFYDLLKNAEAN
AEAKGLDMDKLIIKHVQVNAAPKQRRRTYRAHGRVTAYLSSPSHIEIIVA
EEEVSRVSLKQGAKARNLAARKA

Ligand information

>8eui Chain 1 (length=2855)

uugaccucaaaucagguaggacuacgcgcugaacuuaagcauaucaauaa
gcgcaggaaaagaaaauaaccaugauucccucaguaacggcgagugaagc
gggaaaagcucaaauuugaaaucuggcaacauuucuuuuguuguccgagu
uguaauuucaagaagcugcuuugaguguagacgaucggucuaaguuccuu
ggaacaggacgucagagagggugagaaccccgucuuuggucgauuggaua
ugccauauaaagcgcuuucgaagagucgaguuguuugggaaugcagcucu
aaaugggugguaaauuucaucuaaagcuaaauauuggcgagagaccgaua
gcgaacaaguagagugaucgaaagaugaaaagaacuuugaaaagagaguu
aaauaguacgugaaauugcugaaagggaagcauuggaaaucagucuuaua
ggucagcaucaguuuucgggggcggaaaaagaauaagggaagguggcugu
uuauagcccuuguuguaauacguccacuggggacugaggacugcggcuug
ccaaggaugcugacauaaugguuuucaauggcccgucuugaaacacggac
caaggagucuagcaucuaugcgaguguuugggugaaaaacccauccgcga
aaugaaagugaaugcaggugggaacgcccuggcgugcaccaucgaccgac
ccggaaguuugucaauggaaggguuugaguaagagcauagcuguugggac
ccgaaagauggugaacuaugccugaauagggugaagccagaggaaacucu
gguggaggcucguagagauucugacgugcaaaucgaucuucaaauuuggg
uauaggggcgaaagacuaaucgaaccaucuaguagcugguuccugccgaa
guuucccucaggauagcagaaacucagaucaguuuuaugagguaaagcga
augauuagagguaccuauucucaaacuuuaaauauguaagacgcccuugu
cgcuuaauuggacgugggccaucgaaugagaguuucuagugggccauuuu
ugguaagcagaacuggcgaugcgggaugaaccgaacgugagguuaaggug
ccggaauguacgcucaucagacaccagaaaagguguuaguucgaacuagc
ccugaaaauggauggcgcuuaagcguacuacccauaccucaccgucuggg
uuaagaagcucagacgaguaggcaggcguggagguuugugacgaagccuu
gggcgugagccugggucgaacagccucuagugcagaucuugguggaagua
gcaaauauucaaaugagaacuuugaagacugaaguggggaaagguuccau
gugaacagcaguuggacauggguuagucgauccuaagagauagggaagcu
ccguaugaaaggcacgauuuuucgugccuaucgaaagggaauccgguuaa
uauuccggaaccagaagguggaaucaacacggcaacguaaaugaaguugg
agacgucggcgggagcccugggaagaguucucuuuucuuuuuaacaaacc
acacccugaaaucgguuuauccggagcuaggguaugguguuuggaagagu
ucagcggcugaauccggugcgcucucgacggcccuugaaaauccaacgga
aguguuuucacaucuggucguacucauaaccgcagcaggucuccaaggug
aacagccucuaguugauagaacaauguagauaagggaagucggcaaaaug
gauccguaacuucgggauaaggauuggcucuaaggguaucaacuuagaac
ugguacggacaaggggaaucugacugucuaauuaaaacauagcauugcga
uggccagaaagugguguugacgcaaugugauuucugcccagugcucgaau
gucaaagugaagaaauucaaccaagcgcggguaaacggcaagguagccaa
augccucgucaucuaacuagugacgcgcaugaauggauuaacgagauucc
cacugucccuaucuacuaucuagcgaaaccacagccuggggaacgggcca
ggcaaaaucagcggggaaagaagacccuguugagcuugacucuaguuuga
cauugugaauuacuuaaucaaugaagcggaauuucuagcguuaaaguuuc
gcgaacugauccgcguugaugacauugucagguggggaguuuggcugggg
cggcacaucuguuaaaagauaacgcagguguccuaagggggacucaucga
gaaucucgaguagaauaaaaggguaaaaguccccuugauuuugauuuuca
gugugaauacaaaccaugaaaguguggccuaucgauccuuuguucccucg
aaauuugaggacagaggugccagaaaaguuaccacagggauaacuggcuu
cagccaagacguugcauucuucgaugucggcucuuccuaucauaccgaau
ucgguaagcguuggauuguucacccacuaauagggaacgugagcuggguu
uagaccgucgugagacagguuaguuuuacccuacugaugaagugucgucg
caaugguaauucaacuuaguacgggaaccguugauucagaucauugguau
uugcggcugccugacaaggcaaugccgcggagcuaucaucugccggauaa
cggcugagccagaauccgugccagaaagcgacgauuuauguauaaaaaua
gaguccuguaucguagaagaugggcgaugguugaacggaaguguuuugac
uuguccaugaaauuccagaauccauugcauacgacuuuaauguggaacgg
gguauuguaagcaguagagaucugcugagauuaagccuuuguucccaaga
uuugu

........................<<<<<<.....<......>......>
>>>>.>...<.....<<....>>..<<<<<......<<.....>>.....
>>>>>..<<<...........<<.<<<<<<<.......>>>>>>>.>>..
...........<<<<<.<<<<<..<<<<<..<<<<.<<<<.....<<<<.
.....>>>>.((......<<<.....))>>>......>>>>.>>>>..>>
>>>.....>>>>>>>>.>>........<<<<<<<........>>>>>>>.
....<<<<<..((((((>>>>>..>>>....>..................
...........<<....>>...............<<<<....>>>>....
..............................<<<<<<<<<<<.<...<..>
.<<<<<<<.<<<<<<<<<<<<<<<.....<.<<<<<<<<.....<....>
......>>>>>>>>....>.>>>>>.>>>>>>>>>>........<<<..>
>>.....>>>>>>>....>>>>>>>>>>>>..<<<<........>>>><<
<<<<....<<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>>..
.<<....>>.......<<<<<..<<<<<..>>>>>...>>>>>.<<<<<<
<<....<<<....>>>....>>>.>>>.>>....>>>>>>.>>>>>>..<
<.<...<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>
>>>.....>>>...<<<..<<.<<........>>>>.>>>....>>>>>>
>>>...<<.......>>......>>>>>>......>.>>..<<<<<<...
.<.<<<....>>>.><<<<<<<<<<...<<<.<<<<<<<.....<<<...
.<<<....<<<..>>>....>>>..>.>>.....>>>>>...<<<<.<<<
<.<......>>>>>.>>>>.....>>>>>>>>>>>>>.>>..<<<<.<<<
<<.....>>>>>.>>>>....<<<......>>>...<<<<<<<...<<<(
((.....<<<<<<......<..........<<<.<<<<<<<..>>>>>>>
>>>..........))).>....>>>>>>.>>>...>>>>>>><<<<<<<<
<......>>>>>>>>>....>>>>>><.<<<<<<<<.<<......<<<<.
<<<<....>>>>>>>>...>>>>>>>>>>..>.....>>>>>><<.....
<<.....<<<<<<.......>>>>>><<<<.....<<<<<.<<...<<<<
<....<<.....>>..>>>>>....((<<<<<<...<<....<<<....>
>>........<<<<<<......>>>>>>..>>....>>...<<<<<....
....>>>>>))<<<<..<<<<<<<....<<<....>>>.....((<<<<<
<...<<<<.<<<<<<<.<<<<<<<<<<...[[..>>>>>><...<<<<<.
..<<<<<<....<<<.....>>>....>>>>>>.....>>>>>....><<
<<<<<.>>>>>>>>>>>...>>>>>>>.>>>>.........>>>>>>...
...>>>>.>>>.>>>>>>>>..>>.....<<......>>>>>>>.<<<<.
....>>>>..>>>>..<<<<.....<<<<<<<.<<...............
<<<<<....<....>.....>>>>><<.<<<<<<<<..>>..>>>>>>.>
>............<<<<<<<<<..<<<.........>>>..<<<<<<<..
..<<<<.....>>>>......>>>>>>>...<<...<<..<<<<<<<..<
<<....<<<<<....>>>>.>...>>>>>>>>..>>>>.......<....
.......<<<<<.......>>>>>.....>.....>>....>>.>>>>>>
>.....>>.>>>>>>>..>>>>>>....>>....<<<<<........>>>
>>....<<<<<..<<<.<<......<<<<.<<<<<<..<<<<<.<<<<<<
<<..<<<...>>>...<<<<<.....<<<<..........))..<<<<<.
..>>>>>..>>>>..>>>>>.....>>>>>>>>..>>>>>...<<<....
.>>>..<<<<<<...........>>>>>>..<<...<<<<<<<...<<<<
<<.>>>>>>.....<<...(((>>....>>>>>>>...<<<.<<<<<...
....>>>>.>>>>.<<<....>>>.>>)))......<<<<<<<..<<<<.
))))))>>>>>>>>>>>.........>>>>..>>>>>>.......<<...
<<<<......>>>>..>.>.......<<<<<.<<<<<<..<.<<<<<<..
>>>>>>...>.<<<.........>>>.....>>>>>..>...>>>.>>..
..<<<<<<<....>>>.>>>>....>>..>>>.>>>>>.....<<<<<<<
<..<<<<...<<<<<..............>>>>>..<<<<<...<<<<..
..<<<<<<<<<<]]..>>>>>..>>>>>...>>>>..>>>>>.<<<<...
.<<<<.>>>>....>>>>.>>>>....>>>>>>>>......<<.......
<<.>>....>>........<<<<<....<<<<.<<<......>>>..>>>
>..>>>>>.........<<<<<.<.............>.....<<<<<<<
<<.<<<....<<<<.<....>>>>>...>>>....>>>>.>>>>>...>>
>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8eui Chromatin localization of nucleophosmin organizes ribosome biogenesis.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): R18 Y21 R23 H25 F26 K27 R30 K43 K55 R62 F63 G65 G66 V67 G68 R69 A71 Q72 K74 G77 T79 Q80 R82 W83 P84 V85 K86 N97 A100 N101 H116 Q118 V119 R127 T129 Y130 R131 A132 H133 G134 R135 V136 T137 A138 Y139 L140 S141 R166 S168 L169 K170 A173 K174 R176 N177 R181
• Binding residue (residue number reindexed from 1): R17 Y20 R22 H24 F25 K26 R29 K42 K54 R61 F62 G64 G65 V66 G67 R68 A70 Q71 K73 G76 T78 Q79 R81 W82 P83 V84 K85 N96 A99 N100 H115 Q117 V118 R126 T128 Y129 R130 A131 H132 G133 R134 V135 T136 A137 Y138 L139 S140 R156 S158 L159 K160 A163 K164 R166 N167 R171

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8eui, PDBe:8eui, PDBj:8eui
• PDBsum: 8eui
• PubMed: 36423630
• UniProt: O14339|RL17A_SCHPO Large ribosomal subunit protein uL22A (Gene Name=rpl1701)