Structure of PDB 7unr Chain P Binding Site BS01

Receptor Information

>7unr Chain P (length=120) Species: 208964 (Pseudomonas aeruginosa PAO1)

MRHRKSGRHLSRTSAHRKAMFQNMAVSLFEHELIKTTLPKAKELRRVAEP
LITLAKEDSVANRRLAFDRTRSKAAVGKLFNDLGKRYANRPGGYLRILKC
GFRAGDNAPMAYVELVDRPV

Ligand information

>7unr Chain A (length=2884)

ggucaagugaagaagcgcauacgguggaugccuuggcagucagaggcgau
gaaagacgugguagccugcgaaaagcuucggggagucggcaaacagacuu
ugauccggagaucucugaaugggggaacccaccuaggauaaccuagguau
cuuguacugaauccauaggugcaagaggcgaaccaggggaacugaaacau
cuaaguacccugaggaaaagaaaucaaccgagauucccuuaguaguggcg
agcgaacggggauuagcccuuaagcuucauugauuuuagcggaacgcucu
ggaaagugcggccauagugggugauagccccguacgcgaaaggaucuuug
aagugaaaucgaguaggacggagcacgagaaacuuugucugaacaugggg
ggaccauccuccaaggcuaaauacuacugacugaccgauagugaaccagu
accgugagggaaaggcgaaaagaaccccggagaggggagugaaauagaac
cugaaaccguaugcguacaagcagugggagccuacuuguuaggugacugc
guaccuuuuguauaaugggucagcgacuuauauucaguggcaagcuuaac
cguauaggguaggcguagcgaaagcgagucuuaauagggcguuuagucgc
uggguauagacccgaaaccgggcgaucuauccaugagcagguugaagguu
agguaacacugacuggaggaccgaacccacucccguugaaaagguagggg
augacuuguggaucggagugaaaggcuaaucaagcucggagauagcuggu
ucuccucgaaagcuauuuagguagcgccucauguaucacucuggggggua
gagcacuguuucggcuagggggucaucccgacuuaccaaaccgaugcaaa
cuccgaauacccagaagugccgagcaugggagacacacggcgggugcuaa
cguccgucgugaaaagggaaacaacccagaccgccagcuaaggucccaaa
guugugguuaagugguaaacgaugugggaaggcuuagacagcuaggaggu
uggcuuagaagcagccacccuuuaaagaaagcguaauagcucacuagucg
agucggccugcgcggaagauguaacggggcucaaaccacacaccgaagcu
gcgggugucacggugacgcgguagaggagcguucuguaagccugugaagg
ugaguugagaagcuugcuggagguaucagaagugcgaaugcugacaugag
uaacgacaaugggugugaaaaacacccacgccgaaagaccaaggguuccu
gcgcaacguuaaucgacgcaggguuagucgguuccuaaggcgaggcugaa
aagcguagucgaugggaaacagguuaauauuccuguacuucugguuacug
cgauggagggacggagaaggcuaggccagcuuggcguugguuguccaagu
uuaaggugguaggcugaaaucuuagguaaauccgggguuucaaggccgag
agcugaugacgagucgucuagaugacgaagugguugaugccaugcuucca
agaaaagcuucuaagcuucagguaaccaggaaccguaccccaaaccgaca
cagguggucggguagagaauaccaaggcgcuugagagaacucgggugaag
gaacuaggcaaaauggcaccguaacuucgggagaaggugcgccggcuagg
gugaaggauuuacuccguaagcucuggcuggucgaagauaccaggccgcu
gcgacuguuuauuaaaaacacagcacucugcaaacacgaaaguggacgua
uagggugugacgccugcccggugccggaagguuaauugaugggguuagcg
caagcgaagcucuugaucgaagccccgguaaacggcggccguaacuauaa
cgguccuaagguagcgaaauuccuugucggguaaguuccgaccugcacga
auggcguaacgauggcggcgcugucuccacccgagacucagugaaauuga
aaucgcugugaagaugcaguguauccgcggcuagacggaaagaccccgug
aaccuuuacuguagcuuugcacuggacuuugagccugcuuguguaggaua
ggugggaggcuuugaagcguggacgccaguucgcguggagccauccuuga
aauaccacccuggcaugcuugagguucuaacucugguccguaauccggau
cgaggacaguguauggugggcaguuugacuggggcggucuccuccuaaag
aguaacggaggaguacgaaggugcgcucagaccggucggaaaucggucgc
agaguauaaaggcaaaagcgcgcuugacugcgagacagacacgucgagca
gguacgaaaguaggucuuagugauccggugguucuguauggaagggccau
cgcucaacggauaaaagguacuccggggauaacaggcugauaccgcccaa
gaguucauaucgacggcgguguuuggcaccucgaugucggcucaucacau
ccuggggcugaagccggucccaaggguauggcuguucgccauuuaaagug
guacgcgagcuggguuuagaacgucgugagacaguucggucccuaucugc
cguggacguuugagauuugagaggggcugcuccuaguacgagaggaccgg
aguggacgaaccucugguguuccgguugucacgccaguggcauugccggg
uagcuauguucggaaaagauaaccgcugaaagcaucuaagcgggaaacuu
gccucaagaugagaucucacugggaacuugauuccccugaagggccgucg
aagacuacgacguugauaggcuggguguguaagcguugugaggcguugag
cuaaccaguacuaauugcccgugaggcuugacca

<<<<<<<<......<<<<<<<<<<<....{[[..[[<<<<<<<[[<<...
...<<<<<<.[..(((.>>>.....<<<<<<<..<<<)))...].>>>..
...>>>>>>>...>>>....<<<....>>><<<<<<<....>>>>>>>.<
<<<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<......
>......>>>>>.>.........<<....>>...<<<<<.....<<....
.>>....>>>>>...(((.....<<<<<<..<<<<<..<<<...<<<.<.
.....>.>>>((......<<<.....))>>>...>>>....>>>>>..>>
>>>>...........<<<<<<<<.........>>>>>>>>......<<<<
<..<<<<>>>>>..)))......]].>>>>>>>.]]..............
.<<....>>....]].........<<<<.....>>>>.............
}....>>>>>>>>>>>.......<<<<..<<<<<.....>>>>>..>>>>
...<.<........>.>.<<<<<<....<<<<<<<<<<<<<..<<<...<
<.....>>...>>>...<<....>>..<<<<.....>>>>.....>>>>>
>>>>>>>>..<<.<...<<<<<<.....<<<<<<<<<..<<<....<<<<
<<......>>>>>>.....>>>...<<<.<<.<<........>>.>>>>>
....>>>>>>>>>...<<<.....>>>......>>>>>>......>.>>.
.<<<<<<....<<<<<....>>>>>.<<<<<<<<..<<<<<<<<<.....
<<<...<<<.<<<<...<<.<<<.......>>>..>>...>>>>.>>>..
>>.>.....>>>>>>.>>>....>>>>>>>.>...<<<<<<<<<<.....
.>>>>>>>>>>....<<<......>>>...<<<<.<<<...<<<(((...
..<<<<<<...........<<..<<<<<..<<........<<<<<.<<<.
.<<<<.......>>>>..>>>........<<<......>>>..>>>>>..
..>>..>>>>>.>>...........)))......>>>>>>.>>>...>>>
>>>><<<<<<<.>>>>>>>....>>>>>><.<<<<<...<<<......<<
<<<<<<....>>>>>>>>...>>>..>>>>>..>.....>>>>>><....
..<<.....<<<<<<.......>>>>>><<<<....<<<<<.<<...<<<
<<<...<<.....>>.>>>>>>....((<<<<<<<...<<<...<<<...
.>>>...>>>...>>>..<<<<<........>>>>>))<<<<<<<<<<<<
<...<<<<....<<<<..<<<..<<<<<<<<<<<...((((...>>>>>>
<<..<<<....<<<<<<<<<<<..<<.....>>.>>>>>>>..>>>>...
.>>>...>><..<<<<<<..>>>>>>...>>>>>>...>>>...>>>.>.
.......>>>>...>>....>>>>>>>>>>>>>>>..>>.....<<....
..>>>>>>>.<<<<.....>>>>..>>>>..<<<<....<<<<<<<<.<<
.........<.....<<<<<.....<..>......>>>>><<<<<<<<<<
<<...<<<.....>>>....>>>>>>>>>>>>............<<<<<<
<<<..<<<.........>>>..<<<<<<<.....<<<....>>>......
.>>>>>>>.<<<<<.<<<..<<<<<<<..<<<<.......<<<<<<.<<.
...>>..>>>>>>.......>>>>>>>>>..>>>>><<<<<<.......>
>>>>>........<...........<<<<<<.....>>>>>>.....>..
..>>>>>..>>.>>>>>>>..>..>>.>>>>>>>>.>>>>>>.....>..
...<<<<<........>>>>>..<<<<<<<<.(((.[[......<<<<.[
[<<<<..<<<<<.<<<.<<<<<.<<<<<<<<<<<.<.<............
<<<<...<............<<<<....>>>>.........>........
.....>>>>...>.>.>>>>>>>>>>>...<<<.<<<<<<.....>>>>>
>>>>....>>>>>.>>>..>>>>>..<<<<.....>>>><<<<<<.....
......>>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>..
...<<.[.(((>>....>>>>>>>...<<<<..<<<......>>>..>>>
>.<<<....>>>.>>)))]......<<<<<<<.((..>>>>..))>>>>>
>>.............>>>>..]]>>>>.......<<<<...<<<<<<...
<<.......>>...>>>>>>....>>>.>.......<<<<<.<<<<<<..
<<<<<<<<<......>>>>>>..>>>.<<<<<.....>>>>>.....>>>
>>..>...>>>.>>....<<<<<<<....>>>.>>>>....]]..)))>>
>>>>>>....<<<<<<<...<<<<..<<<<<<<...............>>
>>>>>.<<<<<...<<<<...<<<<<<<<<<<))))>>>>>>..>>>>>.
..>>>>.>>>>>...<<<....<<<<<...........>>>>>....>>>
.>>>>.......>>>>>>>..<<<<<.....>>>>><....<<<<<<<<<
.......>>>>>....<<<<<<<<..<<....<<<<<....>>>>>...>
>...>>>>>.>>>...>>>>..>..>>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7unr Compact IF2 allows initiator tRNA accommodation into the P site and gates the ribosome to elongation
• Resolution: 2.9 Å
• Binding residue (original residue number in PDB): M1 R2 H3 R4 K5 S6 R8 H9 S11 R12 S14 H16 K18 N23 H31 I34 K35 T36 P39 K42 R45 T53 R63 R64 F67 D68 R69 R71 K73 R90 P91 G92 G93 R96 K99 R103 G105 D106 N107
• Binding residue (residue number reindexed from 1): M1 R2 H3 R4 K5 S6 R8 H9 S11 R12 S14 H16 K18 N23 H31 I34 K35 T36 P39 K42 R45 T53 R63 R64 F67 D68 R69 R71 K73 R90 P91 G92 G93 R96 K99 R103 G105 D106 N107

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7unr, PDBe:7unr, PDBj:7unr
• PDBsum: 7unr
• UniProt: O52761|RL17_PSEAE Large ribosomal subunit protein bL17 (Gene Name=rplQ)