Structure of PDB 7ohx Chain P Binding Site BS01

Receptor Information

>7ohx Chain P (length=127) Species: 559292 (Saccharomyces cerevisiae S288C)

NPAKSASARGSYLRVSFKNTRETAQAINGWELTKAQKYLEQVLDHQRAIP
FRRFNRTAQGKEKARWPAKSVKFVQGLLQNAAANAEAKGLDATKLYVSHI
QVNQAPKESSPSHIELVVTEKEEAVAK

Ligand information

>7ohx Chain 1 (length=1561)

guuugaccucaaaucagguaggaguacccgcugaauaucaauaagcggag
gaaaagaaaccaaccgggauugccuuaguaacgcgagugaagcggcaaaa
gcucaaauuugaaaucugguaccuucggugcccgaguuguaauuuggaga
gggcacuuuggggccguuccuugucuauguuccuuggaacaggacgucau
agagggugagaaucccguguggcgaggagugcgguucuuuguaaagugcc
uucgaagagucgaguuguuuggaugcagcucuaagugggugguuccaucu
aaagcuaaauauuggcgagagaccgauagcgaacaguacagugauggaaa
gaugaaaagaacuuugaaaagagagugaaaaaguacgugaaauuguugaa
aggaagggcauuugaucagacaugguguuuugugcccucugcuccuugug
gguaggggaucucgcauuucacugggccagcaucaguuuugguggcagga
uaaauccauaggaauguagcuugccgguaaguauuauagccugugggaau
acugccagcugggacugaggacugcgacguaagucaaggaugcuggcaua
augguuauaugccgcccgucuugaaacacggaccaaggagucuaacgucu
augcgaguguuuggguguaaaacccauacgcguaauugaacguagguugg
ggccucgcaagaggugcacaaucgaccgauccuggauggauuugguaaga
gcauagcuguugggacccgaaagauggugaaaaccaucuaguacugguuc
cugccgaaguuucccucaggauagcagaagcucguaucaguuuuauguaa
gaaugaagagcuuuuagugggccauuuuugguaagcagaacuggcgaugc
gggaugaaccgaacguagaguuaaggugccggaauacacgcucaagaaca
aaauaguucaucuagaccggguggccauggaagucggaauccgcuaagga
guguguaacaaccggaugaacuagcccugggcgcucaagcguguuaccua
uacucuaccgucaggguugauaugaugcccugacgaguaggcaggcgugg
aggucagugacgaagccuagaccguaaggucgggucgaacggccucuagu
gcagaucuuggugguaguagcagcgaaaccacaccaaggggcuuggcuac
cgaagcagaaguaauguuaccgcaauaguaauugaacuuaguacgagagg
aacaguucauucggauaauugguuuuugcggcugucucaggcagccgcga
agcuaccauccgcuggauuauggcugaacccucuaagucagauccaugcu
agaagcggugaugcuccacacagauggauacgaauaaggcguccuugugg
cgucgcugaaccauagcaggcuagcaacggugcacuuggcggaaaggccu
ugggugcuugcuggcgaauugcaaugcguggggauaaaucauuuguauac
gacuuagauguacaacgggguauuguaagcgcugagauuaagccuuuguu
gucugauuugu

..........................<<<<<<...........>>>>>.>
.........<<....>>..<<<<<......<<....>>.....>>>>>..
<<<...........<<.<<<<<<...>>>>>>.>>.............<<
<<<<<<<<<<<<<<<<<<<<<<<<<<....<<<<......>>>>.((...
...<<<.....))>>>..>.>>>>>>>>>..>>>>>>>....>>>>>>>>
>>>........<<<<<<<......>>>>>>>.....<<<<<<..>>>>>>
..>>>.................................<<....>>....
...........<<<......>>>...........................
......<<<<<.<<<<<<.....<<<<...<<<..<<<<<<<<<.....>
>>>>>>>>.....>>>...>>>>..<<<<<<<.<<<<<<<<<<<<<<<..
...<<<<<<<<<......<<<<<....>>>>>.......>>>>>>>.>.>
.>>>>>.>>>>>>>>>>........<<<....>>>.....>>>>>>>...
.>>>>>>.>>>>>..<<<<........>>>><<<<<<....<<<<<<.<<
<<<<..<<<...<<<<.....>>>>...>>>.............<<<<<.
.<<<<<....>>>>>...>>>>>.<<<...<<.....>>....>>>....
>>>>>>.>>>>>>..<<.<...<<<<<<....>>>>>>.....>.>>..<
<<<<<......<<......>>..<<<<<<<<<....<<<.<<<<<..>>>
.>>>>>..>>>>>>>.>>..<<<<.<.<<<.....>>>>..>>>>....<
<<......>>>...<<<<<<<..<<<<(((.....<<<<<<....<....
...<<<<<<<<......<<.....<<.........>>.............
..<<......>>>>.>>>>>>>>......))).>....>>>>>>.>>>>.
.>>>>>>><<<<<<<<<.........>>>>>>>>>....>>>>>><.<<<
<<<<<.<<......<<<<.<<<<....>>>>>>>>...>>>>>>>>>>..
>.....>>>>>><<.....<<.>>....>>...<<<<<...>>>>>.<<<
..........>>>..<<<<<<<<..<<<<.<.<<<<<<............
...>>>>>>.><<<<<...<<<<....<<<<<...........>>>>>..
.>>>>..>>>>>.<<<<...<<<<<..........>>>>>..>>>>.>>>
>...>>>>>>>>.<<<<<<......<<..........<<<<<<.....>>
>>>>......>>.......<<<<<<...<<<<<.<..<<<......>>>.
.>.>>>>>.>>>>>>............>>>>>>.<<<<<<<<........
.....>.....<<<<<<<<<.<<<....<..>...>>>....>>>.>>>>
>>.>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7ohx Analysis of subunit folding contribution of three yeast large ribosomal subunit proteins required for stabilisation and processing of intermediate nuclear rRNA precursors.
• Resolution: 3.3 Å
• Binding residue (original residue number in PDB): S16 A17 R18 S20 Y21 S25 F26 K27 R30 Q34 K43 R62 F63 R69 K74 P84 A85 K86 N97 A100 N101 Q118 V119 Q121 K124 S141 K153 V157 A158 K159
• Binding residue (residue number reindexed from 1): S7 A8 R9 S11 Y12 S16 F17 K18 R21 Q25 K34 R53 F54 R56 K61 P67 A68 K69 N80 A83 N84 Q101 V102 Q104 K107 S109 K121 V125 A126 K127

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000448 cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:0030687 preribosome, large subunit precursor
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7ohx, PDBe:7ohx, PDBj:7ohx
• PDBsum: 7ohx
• PubMed: 34813592
• UniProt: P05740|RL17A_YEAST Large ribosomal subunit protein uL22A (Gene Name=RPL17A)