Structure of PDB 7ohw Chain P Binding Site BS01

Receptor Information

>7ohw Chain P (length=137) Species: 559292 (Saccharomyces cerevisiae S288C)

NPAKSASARGSYLRVSFKNTRETAQAINGWELTKAQKYLEQVLDHQRAIP
FRRFNSSIGRTAQGKEFGVTKARWPAKSVKFVQGLLQNAAANAEAKGLDA
TKLYVSHIQVNQAPKESSPSHIELVVTEKEEAVAKAA

Ligand information

>7ohw Chain 1 (length=1639)

guuugaccucaaaucagguaggaguacccgcugaacuuauaucaauaagc
ggaggaaaagaaaccaaccgggauugccuuaguaacgcgagugaagcggc
aaaagcucaaauuugaaaucugguaccuucggugcccgaguuguaauuug
gagagggcaacuuuggggccguuccuugucuauguuccuuggaacaggac
gucauagagggugagaaucccguguggcgaggagugcgguucuuuguaaa
gugccuucgaagagucgaguuguuugggaaugcagcucuaaguggguggu
uccaucuaaagcuaaauauuggcgagagaccgauagcgaacaaguacagu
gauggaaagaugaaaagaacuuugaaaagagagugaaaaaguacgugaaa
uuguugaaagggaagggcauuugaucagacaugguguuuugugcccucug
cuccuuguggguaggggaaucucgcauuucacugggccagcaucaguuuu
gguggcaggauaaauccauaggaauguagcuugccucgguaaguauuaua
gccugugggaauacugccagcugggacugaggacugcgacguaagucaag
gaugcuggcauaaugguuauaugccgcccgucuugaaacacggaccaagg
agucuaacgucuaugcgaguguuuggguguaaaacccauacgcguaauga
acgagguuggggccucgcaagaggugcacaaucgaccgauccugaggaug
gauuugguaagagcauagcuguugggacccgaaagauggugaaaaccauc
uaguagcugguuccugccgaaguuucccucaggauagcagaagcucguau
caguuuuaugaaaaugaagagcuuuuagugggccauuuuugguaagcaga
acuggcgaugcgggaugaaccgaacguagaguuaaggugccggaauacac
gcucaucagacacaaaagguguuaguucaucuagacagccggacgguggc
cauggaagucggaauccgcuaaggaguguguaacaacucaccggccgaau
gaacuagcccugaggauggcgcucaagcguguuaccuauacucuaccguc
aggguugauaugaugcccugacgaguaggcaggcguggaggucagugacg
aagccuagaccguaaggucgggucgaacggccucuagugcagaucuuggu
gguaguagcagcgaaaccacagccaaggaacgguugggcuugggcaguca
agcgcgacauugcuauaccgaagcagaauucgguaauguuaccgcaauag
uaauugaacuuaguacgagaggaacaguucauucggauaauugguuuuug
cggcugucuaucaggcauugccgcgaagcuacauccgcuggauuauggcu
gaacgccucuaagucagaauccaugcuagaacgcggugaugcuccacaca
gauggauacgaauaaggcguccuuguggcgucgcugaaccauagcaggcu
agcaacggugcacuuggcggaaaggccuugggugcuugcuggcgaauugc
aaugcguggggauaaaucauuuguauacgacuuagauguacaacggggua
uuguaagcagcugcugagauuccuuuguugucugauuug

..........................<<<<<<.....<..>......>>>
>>.>.........<<....>>..<<<<<......<<....>>.....>>>
>>..<<<..<........<<.<<<<<<...>>>>>>.>>........>..
..<<<<<<<.<<<<<<<<<<<<<<<<<<<<.....<<<<......>>>>.
((......<<<.....))>>>....>>>>>>>>>..>>>>>>>....>>>
>>>>>>>>........<<<<<<<........>>>>>>>.....<<<<<<.
.>>>>>>..>>>..................................<<..
..>>...............<<<......>>>...................
...............<<<<<.<<<<<<.....<<<<...<<<..<<<<<<
<<<.....>>>>>>>>>......>>>...>>>>..<<<<<<<.<<<<<<<
<<<<<<<<.....<<<<<<<<<......<<<<<......>>>>>......
.>>>>>>>>..>.>>>>>.>>>>>>>>>>........<<<....>>>...
..>>>>>>>....>>>>>>.>>>>>..<<<<........>>>><<<<<<.
...<<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>>.......
....<<<<<..<<<<<....>>>>>...>>>>>.<<<<<<<<.......>
>>>.>>>>....>>>>>>.>>>>>>..<<.<..<<<<<<<....>>>>>>
>.....>.>>..<<<<<<....<.<........>.><<<<<<<<<....<
<<.<<<<...>>>>>>>..>>>>>>>.>>..<<<<.<<<.<.....>.>>
>.>>>>....<<<......>>>...<<<<<<<..<<<<(((.....<<<<
<<.....<<.......<<<.<<<<<<<<<<.......<<<<<.<<....<
<.........>>....>>.........<<......>>...>>>>>...>>
>>>>>>>>>>>......))).>>...>>>>>>.>>>>..>>>>>>><<<<
<<<<<.........>>>>>>>>>....>>>>>><.<<<<<<<<.<<....
..<<<<.<<<<....>>>>>>>>...>>>>>>>>>>..>.....>>>>>>
<<.....<<.>>....>>....<<<<.......>>>>.....<<<<<...
.<..>....>>>>>.<<<<<<<......>>>>>>>..<<<<<<<<..<<<
<.<.<<<<<<...............>>>>>>.><<<<<...<<<<....<
<<<<<<<.<...>.>>>..>>>>>...>>>>.>>>>>.<<<<...<<<<<
...........>>>>>...>>>>.>>>>....>>>>>>>>.<<<<<<...
...<<..........<<<<<<.....>>>>>>......>>.......<<<
<<<...<<<<<.<..<<<......>>>..>.>>>>>.>>>>>>.......
.....>>>>>>.<<<<<<.<..<.......>..>.....<<<<<<<<<.<
<<....<<<..>>>...>>>.>>>.>>>>>>..>>>>>>

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7ohw Analysis of subunit folding contribution of three yeast large ribosomal subunit proteins required for stabilisation and processing of intermediate nuclear rRNA precursors.
• Resolution: 3.5 Å
• Binding residue (original residue number in PDB): S16 R18 S20 Y21 S25 F26 K27 R30 Q34 R62 F63 S65 S66 R69 K74 P84 Q96 N97 A100 N101 H116 Q118 V119 Q121 K124 S141 K153 A158 K159
• Binding residue (residue number reindexed from 1): S7 R9 S11 Y12 S16 F17 K18 R21 Q25 R53 F54 S56 S57 R60 K65 P75 Q87 N88 A91 N92 H107 Q109 V110 Q112 K115 S117 K129 A134 K135

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000448 cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:0030687 preribosome, large subunit precursor
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7ohw, PDBe:7ohw, PDBj:7ohw
• PDBsum: 7ohw
• PubMed: 34813592
• UniProt: P05740|RL17A_YEAST Large ribosomal subunit protein uL22A (Gene Name=RPL17A)