Structure of PDB 7a18 Chain P Binding Site BS01

Receptor Information

>7a18 Chain P (length=129) Species: 243230 (Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539)

QTFRNKKQRKQQVKLRKPGFAVAKYVRMSPRKVRLVVDVIRGKSVQDAED
LLRFIPRSASEPVAKVLNSAKANALHNDEMLEDRLFVKEAYVDAGPTLKR
LIPRARGSANIIKKRTSHITIIVAEKGNK

Ligand information

>7a18 Chain X (length=2699)

ggucaagauaguaaggguccacgguggaugcccuggcgcuggagccgaug
aaggacgcgauuaccugcgaaaagccccgacgagcuggagauacgcuuug
acucggggauguccgaauggggaaacccaccucguaagagguauccgcaa
ggaugggaacucagggaacugaaacaucucaguaccugaaggagaagaaa
gagaauucgauuccguuaguagcggcgagcgaacccggaucagcccaaac
cgaaacgcuugcguuucgggguuguaggaccaucaagccgaaguggcugg
aaagcuacaccucagaaggugagaguccuguaggcgaacgagcccugagu
aggucguuguucgugaaacgaugacugaauccgcgcggaccaccgcgcaa
ggcuaaauacucccagugaccgauagcgcauaguaccgugagggaaaggu
gaaaagaaccccgggaggggagugaaagagaaccugaaaccguggacuua
caagcagucauggcaccuuaugcguguuauggcgugccuauugaagcaug
agccggcgacuuagaccugacgugcgagcuuaaguugaaaaacggaggcg
gagcgaaagcgaguccgaauagggcggcauuaguacgucgggcuagacuc
gaaaccaggugagcuaagcaugaccagguugaaacccccgggggcggagg
accgaaccggugccugcugaaacagucucggaugaguuguguuuaggagu
gaaaagcuaaccgaaccuggagauagcuaguucuccccgaaauguauuga
gguacagccucggauguugaccauguccuguagagcacucacaaggcuaa
aaccuuaugaaacuccgaaggggcacgcguuuaguccgggagugaggcug
cgagagcuaacuuccguagccgagagggaaacaacccagaccaucagcua
aggucccuaaaugaucgcucagugguuaaggaugugucgucgcauagaca
gccaggagguuggcuuagaagcagccacccuucaaagagugcguaauagc
ucacuggucgagugacgaugcgccgaaaaugaucggggcucaagugaucu
accgaagcuauggauucaacucggaguugucugguaggggagcguucagu
ccgcggagaagccauaccggaaggagugguggagccgacugaagugcgga
ugccggcaugaguaacgauaaaagaagugagaaucuucuucgccguaagg
acaaggguuccuggggaagggucguccgcccagggaaagucgggaccuaa
ggugaggccgaacggcgcagccgauggacagcaggucaagauuccugcac
cgaucauguggagugauggagggacgcauuacgcuauccaaugccaagcu
auggcuaugcugguugguacgcucaagggcgaucgggucagaaaaucuac
cggucugccucagacguaucgggagccgaaguuggaaacgcgacggugcc
aagaaaagcuucuaaacguugaaacaugauugcccguaccgcaaaccgac
acagguguccgagugucaaugcacuaaggcgcgcgagagaacccucguua
aggaacuuugcaaucucaccccguaacuucggaagaagggguccccacgc
ugcguggggcgcagugaauaggcccaggcgacuguuuaccaaaaucacag
cacucugccaacacgaacaguggacguauagggugugacgccugcccggu
gccggaaggucaaguggagcggugcaagcugcgaaaugaagccccgguga
acggcggccuaagguagcgaaauuccuugucggguaaguuccgaccugca
cgaaaggcguaacgaucugggcgcugucucaacgagggacucggugaaau
ugaauuggcuguaaagaugcggccuacccguagcaggacgaaaagacccc
guggagcuuuacuauagucuggcauugggauucggguuucuagaaacuug
gauuucuaaccugaaaaaucacuuucggggaccgugcuuggcggguaguu
ugacuggggcggucgccucccaaaauguaacggaggcgcccaaaggucac
cucaagacgguuggaaaucgucuguagagcgcaaagguagaagguggcuu
gacugcgagacugacacgucgagcagggaggaaacucgggcuuagugaac
cggugguaccguguggaagggccaucgaucaacggauaaaaguuaccccg
gggauaacaggcugaucucccccgagaguccauaucggcggggagguuug
gcaccucgaugucggcucgucgcauccuggggcugaagaaggucccaagg
guugggcuguucgcccauuaaagcggcacgcgagcuggguucagaacguc
gugagacaguucggucucuauccgcuacgggcgcaggagaauugagggga
guugcuccuaguacgagaggaccggagugaacggaccgcuggucucccug
cugucguaccaacggcacaugcaggguagcuauguccggaacggauaacc
gcugaaagcaucuaagcgggaagccagccccaagaugaguucucccacug
aagguaagacucccggaagaccaccggguuaagaggccaggcgugcacgc
auagcaauguguucagcggacuggugcucaucagucgaggucuugacca

<<<<<<<<......<<<<<<<<<<<.....<<..<<<<<<<<<<<<....
..<<<<<<<((..((>>>>.....<<<<<<...<<.))..))..>>....
..>>>>>>...>>>....<<<....>>><<<<......>>>><<<<....
>>>>>>...<<<<<<.....<......>......>>>>>.>.........
<<....>>...<<<<......<<.....>>.....>>>>...<<<....<
<<<<<<<....>>>>>>>>......<<<....<<..<<<...<<<<<...
...>>>>>((......<<<.....))>>>...>>>....>>..>>>....
.<<<<<<<<.........>>>>>>>>......<<<<<..((((>>>>>..
>>>......>>.>>>>>>.>>..............<<....>>....>>.
........<<<<....>>>>..................>>>>>>>>>>>.
......<<<<<<<<<<.......>>>>>>>>>>...<............>
.<<<<<<....<<<.<<<<<<<<<<..<<<...<<<....>>>...>>>.
..<<....>>...<<......>>.........>>>>>>>>>>>>>..<<.
<...<<<<<<.....<<<<<<<<<..<<<.....<<<<..>>>>......
>>>...<<<..<.<<........>>.>.>>>....>>>>>>>>>...<<<
.....>>>......>>>>>>......>.>>..<<<<<<....<<<<<...
.>>>>><<<<<<<<<...<<<<.<<<<<<...<<<...<<<.<<<<....
..>>>>.>>>..>>.>...>>>>>>.>.>>>..>>>>>>>.>>..<<<<<
<<.<<......>>.>>>>>>>....<<<......>>>...<<<<.<<<..
.<<<(((.....<<<<<<...........<<..<<<<<<<<<........
<<<<<.<<.....<.........>.....>>..........<......>.
...>>>>>....>>>>>>>>>.>>..........)))......>>>>>>.
>>>...>>>>>>><..<<<<<...>>>>>..>....>>>>>><.<<<<<<
<.<<......<<<<<.<<....>>.>>>>>...>>.>>>>>>>..>....
.>>>>>><......<<.....<<<<<<.......>>>>>><<<<....<<
<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<.<<...
<<<...<<<<..>>>>...>>>...>>...<<<<<........>>>>>))
<<<<<<<<<...<<...<<<<....<<<<..<<<..<<<<<.<<<<.<<.
..[[....>>>>>><<<<<<<.<<..<<<<<<<<<<...<<.....>>.>
>>>>>.>>>>..>>>>>>>>>..........>>>>>...>>>...>>>>.
........>>>>...>>......>>>>>>>>>>>>>..>>.....<<...
...>>>>>>>.<<<<<....>>>>>..>>>>..<<<<....<<<<<<<<.
<<...............<<<<<....<....>.....>>>>><<<<<<<<
.>>>>>>>>............<<<<<<<<<..<<...........>>..<
<<<<<<.....<<<.....>>>.......>>>>>>>.<<<<<.<<<..<<
<<<<<..<<<...<<...<<<<<....>>>>>...>>...>>>>>>>>..
>>>>>...........<...........<<<<<<.....>>>>>>.....
>....>>>>>..>>.>>>>>>>.....>>.>>>>>>>>.>>>>>>.....
>.....<<<<<........>>>>>..<<<<<<<<.<<<.........<<<
<.<<<<<<..<<<<<.<<<.<<<<<.<<<<<<<..<<<......>>>..>
>>>>>>...<<<<<<.......>>.>>>>....>>>>>.>>>..>>>>>.
.<<<<.....>>>><<<<<<...........>>>>>>..<<...<<<<<<
<...<<<<<<.......>>>>>>.....<<...(((>>....>>>>>>>.
..<<<<..<<<......>>>..>>>>.<<<....>>>.>>))).......
<<<<<<<.<<..))))..>>>>>>>>>.............>>>>..>>>>
>>.......<<<<...<<<<<<...<<.......>>...>>>>>>....>
>>.>.......<<<<<.<<<<<<..<<<<<<<<<......>>>>>>>..>
>.<<<<<.....>>>>>.....>>>>>..>...>>>.>>....<<<<<<<
....>>>.>>>>........>>>>>>>>>>>....<<<<<<<...<<<<.
.<<<<<<<...............>>>>>>>.<<<<<...<<<<...<<<<
<<<<<<<.]].>>>>>>...>>>>>...>>>>.>>>>>....<<....<<
<<<...........>>>>>....>>..>>>>.......>>>>>>>..<<.
.>>....<<<<<<<<<.......>>>>>....<<<<<<<<.<<<....<<
<<<....>>>>>...>>>..>>>>>.>>>...>>>>....>>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7a18 Ribosome-binding and anti-microbial studies of the mycinamicins, 16-membered macrolide antibiotics from Micromonospora griseorubida.
• Resolution: 3.4 Å
• Binding residue (original residue number in PDB): R9 K11 K12 R14 K15 Q16 K19 R21 K22 V27 Y30 R32 S34 R36 K37 R62 K70 N73 S74 A77 N78 H81 N82 D98 A99 R105 P108 R109 A110 R111 G112 S113 A114 N115 I117 K118 K119 R120
• Binding residue (residue number reindexed from 1): R4 K6 K7 R9 K10 Q11 K14 R16 K17 V22 Y25 R27 S29 R31 K32 R57 K65 N68 S69 A72 N73 H76 N77 D93 A94 R100 P103 R104 A105 R106 G107 S108 A109 N110 I112 K113 K114 R115

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7a18, PDBe:7a18, PDBj:7a18
• PDBsum: 7a18
• PubMed: 34417608
• UniProt: Q9RXJ7|RL22_DEIRA Large ribosomal subunit protein uL22 (Gene Name=rplV)