Structure of PDB 4wj3 Chain P Binding Site BS01

Receptor Information
>4wj3 Chain P (length=589) Species: 208964 (Pseudomonas aeruginosa PAO1) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
MRSHYCGQLNESLDGQEVTLCGWVHRRRDHGGVIFLDVRDREGLAQVVFD
PDRAETFAKADRVRSEFVVKITGKVRLRPEGARNPNMASGSIEVLGYELE
VLNQAETPPFPLDEYSDVGEETRLRYRFIDLRRPEMAAKLKLRARITSSI
RRYLDDNGFLDVETPILGRPTPEGARDYLVPSRTYPGHFFALPQSPQLFK
QLLMVAGFDRYYQIAKCFRDEDLRADRQPEFTQIDIETSFLDESDIIGIT
EKMVRQLFKEVLDVEFDEFPHMPFEEAMRRYGSDKPDLRIPLELVDVADQ
LKEVEFKVFSGPANDPKGRVAALRVPGAASMPRSQIDDYTKFVGIYGAKG
LAYIKVNERAKGVEGLQSPIVKFIPEANLNVILDRVGAVDGDIVFFGADK
AKIVCDALGALRIKVGHDLKLLTREWAPMWVVDFPMFEENDDGSLSALHH
PFTSPKCTPAELEANPGAALSRAYDMVLNGTELGGGSIRIHDKSMQQAVF
RVLGIDEAEQEEKFGFLLDALKYGAPPHGGLAFGLDRLVMLMTGASSIRE
VIAFPKTQSAGDVMTQAPGSVDGKALRELHIRLREQPKA
Ligand information
>4wj3 Chain T (length=76) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
uccgcgauagcucagucgguagagcaaaugacuguuaaucauuggguccc
ugguucgaguccaggucgcggagcca
<<<<<<<..<<<<........>>>>.<<<<<.......>>>>>.....<<
<<<.......>>>>>>>>>>>>....
Receptor-Ligand Complex Structure
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PDB4wj3 Structure of the Pseudomonas aeruginosa transamidosome reveals unique aspects of bacterial tRNA-dependent asparagine biosynthesis
Resolution3.705 Å
Binding residue
(original residue number in PDB)		R27 R29 D30 H31 G33 V34 F36 R65 A83 N85 E94 Y116
Binding residue
(residue number reindexed from 1)		R26 R28 D29 H30 G32 V33 F35 R64 A82 N84 E93 Y115
Enzymatic activity
Catalytic site (original residue number in PDB) R220 E222 R228 Q229 E483 G486 R538
Catalytic site (residue number reindexed from 1) R219 E221 R227 Q228 E482 G485 R537
Enzyme Commision number 6.1.1.23: aspartate--tRNA(Asn) ligase.
Gene Ontology
Molecular Function
GO:0000166 nucleotide binding
GO:0003676 nucleic acid binding
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004815 aspartate-tRNA ligase activity
GO:0005524 ATP binding
GO:0016874 ligase activity
GO:0050560 aspartate-tRNA(Asn) ligase activity
Biological Process
GO:0006412 translation
GO:0006418 tRNA aminoacylation for protein translation
GO:0006422 aspartyl-tRNA aminoacylation
Cellular Component
GO:0005737 cytoplasm

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Biological Process
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Cellular Component
External links
PDB RCSB:4wj3, PDBe:4wj3, PDBj:4wj3
PDBsum4wj3
PubMed25548166
UniProtQ51422|SYDND_PSEAE Aspartate--tRNA(Asp/Asn) ligase (Gene Name=aspS)

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