Structure of PDB 3j3w Chain P Binding Site BS01

Receptor Information

>3j3w Chain P (length=112) Species: 224308 (Bacillus subtilis subsp. subtilis str. 168)

KLIEDITKEQLRTDLPAFRPGDTLRVHVKVVEGNRERIQIFEGVVIKRRG
GGISETFTVRKISYGVGVERTFPVHTPKIAKIEVVRYGKVRRAKLYYLRE
LRGKAARIKEIR

Ligand information

>3j3w Chain A (length=2685)

gguuaaguuagaaagggcgcacgguggaugccuuggcacuaggagccgau
gaaggacgggacgaacaccgauaugcuucggggagcuguaagcaagcuuu
gauccggagauuuccgaauggggaaacccaccacucguaauggaguggua
uccauaucugaauucauaggauaugagaaggcagacccggggaacugaaa
caucuaaguacccggaggaagagaaagcaaaugcgauucccugaguagcg
gcgagcgaaacgggauuagcccaaaccaagaggcuugccucuugggguug
uaggacacucuguacggaguuacaaaggaacgagguagaugaagaggucu
ggaaaggcccgccauaggagguaacagcccuguagucaaaacuucguucu
cuccugaguggauccugaguacggcggaacacgugaaauuccgucggaau
ccgggaggaccaucucccaaggcuaaauacucccuagugaccgauaguga
accaguaccgugagggaaaggugaaaagcaccccggaaggggagugaaag
agauccugaaaccgugugccuacaaguagucagagcccguuaacggguga
uggcgugccuuuuguagaaugaaccggcgaguuacgaucccgugcaaggu
uaagcagaagaugcggagccgcagcgaaagcgagucugaauagggcgcau
gaguacguggucguagacccgaaaccaggugaucuacccauguccagggu
gaaguucagguaacacugaauggaggcccgaacccacgcacguugaaaag
ugcggggaugagguguggguaggggugaaaugccaaucgaaccuggagau
agcugguucucuccgaaauagcuuuagggcuagccucaagguaagagucu
uggagguagagcacugauuggacuaggggccccuaccggguuaccgaauu
cagucaaacuccgaaugccaaugacuuauccuugggagucagacugcgag
ugauaagauccguagucgaaagggaaacagcccagaccgccagcuaaggu
cccaaaguauacguuaaguggaaaaggauguggaguugcuuagacaacca
ggauguuggcuuagaagcagccaccauuuaaagagugcguaauagcucac
uggucgagugacucugcgccgaaaauguaccggggcuaaacguaucaccg
aagcugcggacuguucuucgaacagugguaggagagcguucuaagggcug
ugaagccagaccggaaggacugguggagcgcuuagaagugagaaugccgg
uaugaguagcgaaagaggggugagaauccccuccaccgaaugccuaaggu
uuccugaggaaggcucguccgcucaggguuagucgggaccuaagccgagg
ccgaaaggcguaggcgauggacaacagguugauauuccuguaccaccucc
ucaccauuugagcaauggggggacgcaggaggauaggguaagcgcgguau
uggauauccgcguccaagcaguuaggcugggaaauaggcaaauccguuuc
ccauaaggcugagcugugauggcgagcgaaauauaguagcgaaguuccug
auuccacacugccaagaaaagccucuagcgaggugagaggugcccguacc
gcaaaccgacacagguaggcgaggagagaauccuaaggugaucgagagaa
cucucguuaaggaacucggcaaaaugaccccguaacuucgggagaagggg
ugcucuguuagggugcaagcccgagagagccgcagugaauaggcccaggc
gacuguuuagcaaaaacacaggucucugcgaagccguaaggcgaaguaua
ggggcugacgccugcgcggccguaacuauaacgguccuaagguagcgaaa
ggcgcaacgaucugggcacugucucaacgagagacucggugaaauuauag
uaccugugaagaugcagguuacccgcgacaggacggaaagaccccgugga
gcuuuacugcagccugauauugaauguugguacagcuuguacaggauagg
uaggagccuuggaaaccggagcgccagcuucgguggaggcaucgguggga
uacuacccuggcuguauugaccuucuaacccgccgcccuuaucgggcggg
gagacagugucaggugggcaguuuggugauccggugguuccgcauggaag
ggccaucgcucaacggauaaaagcuaccccggggauaacaggcuuaucuc
ccccaagaguccacaucgacggggagguuuggcaccucgaugucggcuca
ucgcauccuggggcuguagucagucccaaggguugggcuguucgcccauu
aaagcgguacgcgagcuggguucagaacgucgugagacaguucggucccu
auccgucgcgggcgcaggaaauuugagaggagcuguccuuaguacgagag
gaccgggauggacgcaccgcugguguaccaguuguucugccaagggcauc
gcuggguagcuaugugcggacgggauaagugcugaaagcaucuaagcaug
aagccccccucaagaugagauuucccauuccgcaaggaaguaagaucccu
gaaagaugaucagguugauaggucugagguggaaguguggcgacacaugg
agcugacagauacuaaucgaucgaggacuuaacca

<<<<<<<<......<<<<<<<<<<<.....<<.<<<<<<<.<<<<<<...
...<<<<<<.....((.>>>.....<<<<<<<..<<<)).....>>>...
..>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.
<<<<<<<<<.........>>>>>>>.>>.>>....<<<<<<.....<...
...>......>>>>>.>.........<<....>>...<<<<......<<.
....>>.....>>>>...<<<....<<<<<<<<....>>>>>>>>.....
.<<<.<<<<<...................<.<<<<<.<<<.....<<<<.
.....>>>>.........<<<.......>>>...>>>...>>>>>..>..
.....>>>>>...>>>.....<<<<<<<<.........>>>>>>>>....
..<<<<<..((((>>>>>..>>>......>>.>>.>>>>.>>>.......
.......<<....>>....>>.........<<<<....>>>>........
..........>>>>>>>>>>>.......<<<<..<<<<......>>>>..
>>>>...<............>.<<<<<<....<<<<<<<.<<<<...<<<
...<<<.....>>>...>>>...<<....>>..<<<<.....>>>>....
...>>>>.>>>>>>>..<<.<...<<<<<<....<<<<<<<<<<..<<<.
...<<<<<<......>>>>>>.....>>>...<<<.<<<<<........>
>>>>>>>....>>>>>>>>>>..<<<.....>>>......>>>>>>....
..>.>>..<<<<<<....<<<<<....>>>>><<<<<<<<<......<.<
<<<.<...<<<...<<<<<<<<...<<.<<<.......>>>..>>...>>
>>>>>>..>>.>...>.>>>>.>......>>>>>>>.>>..<<<<<<<.<
<......>>.>>>>>>>....<<<......>>>....<<<.<<<...<<<
(((.....<<<<<<...........<<..<<<<<<<<<............
........<...........>...............<<......>>....
........>>>>>>>>>.>>...........))).....>>>>>>.>>>.
..>>>>>>..<<<<<.....>>>>>.....>>>>>><.<<<<<<..<<..
....<<<<<..........>>>>>...>>..>>>>>>..>.....>>>>>
><......<.....<<<<<<.......>>>>>><<<<....<<<<<.<<<
..<<<<<<...<<.....>>.>>>>>>....((<<<<.<<...<<<...<
<<....>>>...>>>...>>...<<<<<........>>>>>))<<<<..<
<<<<<...........<<<<....<<<<..<<<..<<<....<<<<<...
[[[....>>>>>.<<<...<.....<.<<<<<<<<.<<.....>>>>>>>
>>.....>.>......>..>>>......<.......>.........>>>.
..>>>...>>>>.........>>>>......>>>>>>.>>>>>>>>.>>>
.....<<......>>>>>>>.<<<<.....>>>>..>>>>..<<<<....
<<<<<<<<.<<...............<<<<<...............>>>>
><<<<<.<<..<<<....>>>.>>.>>>>>............<<<<<<<<
<..<<...........>>..<<<<<<<.....<<<....>>>.......>
>>>>>>..<<<......<<<<<<.......>>>>>>..............
.>>>...>>.>>>>>>>.....>>.>>>>>>>>.>>>>.>.....>....
.<<<<<........>>>>>..<<<<<<<<.<<<.........<<<<.<<<
<<<..<<<<<.<<<<<<<<<.<<<.<<<.<<<<<<<..............
<<...<.........<<<<<<<....>>>>>>>......>..........
...>>.....>>>>>>>.>>>.>>>....<<.<<<<<<.....>>>>>>>
>.....>>>>>>>>>..>>>>>.........<<<<<<.<<<..))))..>
>>>>>>>>.............>>>>..>>>>>>.......<<<<...<<<
<<<...<<.......>>...>>>>>>....>>>.>.........<<<.<<
<<<<..<<<<<<<<<......>>>>>>>..>>.<<<<<.....>>>>>..
...>>>>>..>...>>>.......<<<<<<<....>>>.>>>>.......
.>>>>>>>>>>>....<<<<<<<...<<<<..<<<<<<<...........
....>>>>>>>.<<<<<...<<<<...<<<<.<<<<<<.]]]>>>>>>..
.>>>>...>>>>.>>>>>...<<<.<..<<<<<...........>>>>>.
..>>>>.>>>>.......>>>>>>>....<......>......<<<<<<<
<.........>>>>....<<<<<<<<..<<....<<<<<....>>>>>..
.>>...>>>>>.>>>...>>>>....>>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 3j3w Cryo-EM structures of the late-stage assembly intermediates of the bacterial 50S ribosomal subunit
• Resolution: 10.7 Å
• Binding residue (original residue number in PDB): K3 R21 I55 S56 E57 T58 H77 R88 K91 R93 R94 A95 K96 L97 Y98 Y99 L100 R101 E102 R109 R114
• Binding residue (residue number reindexed from 1): K1 R19 I53 S54 E55 T56 H75 R86 K89 R91 R92 A93 K94 L95 Y96 Y97 L98 R99 E100 R107 R112

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:3j3w, PDBe:3j3w, PDBj:3j3w
• PDBsum: 3j3w
• PubMed: 23700310
• UniProt: O31742|RL19_BACSU Large ribosomal subunit protein bL19 (Gene Name=rplS)