Structure of PDB 7qep Chain O5 Binding Site BS01

Receptor Information

>7qep Chain O5 (length=121) Species: 284813 (Encephalitozoon cuniculi GB-M1)

KIEASALRQLGIKQIEERAAEIKADLAALRQKKNSGDVGANDIKTAKKNL
ARALTVRREKILEELVEAYRGTPVSKLPKELRPKLNRSKRRALTKTQLRR
KTRRQRARMSKFPRVIFAYNE

Ligand information

>7qep Chain 1 (length=2319)

ucucuguucacgggauugauuggcauuagcggcgaugaaggacgugcagg
aggacgauaugcguuguuguugugacggugucugaauugugugcggugug
cacggggaccccuuggacuuaagcauaucaguaaagggaggagaagaaac
caaaugggauugccugaguagcggcgagugaaauggcaugagccugugug
guaacugcguggcggguuguguuuguggcagcagcggcgagugcgauuga
auucggaugcggagagggcgaugugccuguaggcgcggcggcuuuuuuug
ugaguaguguugcuugguagugcagcgcgaaugcggugguggaugcaccg
aaggcuaaauauggcugaugaccgauagcggauaaguagagugauugaag
ugugaaaaggaggaugaugagaaaugaaagugaauggagugaauggggga
uguucccgcccgucuugaaacacggaccaaggagacgacggcaggauugu
uuuccgagaggggaggaugcugcggucggacccgaaagauggugagcuau
guguguguugauugaagggaugcgaaaguguucuggaggaucuaagccgu
ucugacgugcaacucgauggcaugacaugugcauaggggcgaaagaccaa
acgaaccaucugguagcugguucccucugaaaugucucucaggacagcgg
cgacggguguaagauguuguaaagcacuaaugcgcugcguuaaacuuuga
agaugucuuuuaaguuguugccagugggcauacugugguaagcauggugu
gcgagaagggaugaaccuuuaagcgggcuaaggagucuuaugcaagguga
auccggcggccugauuuugacagccgaauugggcggcggagaaaauggaa
gggauugaauugcauuccgaugccugcgggggaguaucugaguaaggggg
cgguaucguucugcugaagguccugcgugagcggggguggaggggcgaua
ugugcagaucuugguagcaguaacaaggaaaugauuugcgcuggacgagg
guucccuguggcaggggugaaucgacuguggauacggaggugcauugaua
guuguguguauugcggcugggaggcgacuuggcugugacggaugggggcg
gcccuguccaggauagcagaacuggaugucugcugcggauuguuaugaug
cgaagcagcgaugggcgagccuguuccgaugaaaaagaggcugcuuuguc
guaccuagaccgcaucugguguccacuguuugccgcggaaguguuuugug
caagggccguaagggaagucggcaagauugauguguaacuucgggagaag
gauuggcucuagcgugcuagaacuguuuguuguggggaaucugacuguuu
acuaaaaacauagcuuuguguguggacgcaaagugaauucugcccagugc
acuuuuaguuacggccuguugggcuaagcgaguguaaacggcgggaguaa
cuaugacucucuuaagguagccaaaugccucgucauguaauuagugacgc
gcaugaauggagcaacgagauucccacugucccuacggccugucuuguga
accuacagccaagggaacgggcuugggcugaucagcggggaaagaagacc
cuguugagcuugacucuagcaugugugaagugcugggugcccagugcuuu
guggauggauguggggaguuuggcuggggcggcacgacugcuaaagacua
acgcagacguccuaaggcgggcucagcggggaaggaaaccacgcguggag
cacaagggcacaagccugcuugagacuguuccuauugcgggacuguuugg
gggaaacccuggccuagagaucccauguccuauugugcgugggacauggg
ugacagaaaaguuaccacagggauaacuggcuuguagcagccgagcgacc
auagcgacgcugcuuuuugauucuucgaugucggcucuuccuaucaucgc
gaagcagagauugcgaaguguuggauuguucacccaccaacagggaacgu
gagcuggguuuagaccgucgugagacagguuaguuuuacccuacugugug
gacugugcagcagaucuugauaguacgagaggaacucuugauugcgaccu
cugguggagcggcgugccugcuggcugcugcggagcuacgucgggaguau
aauggcugaaggccucuaagccugaagaccgacugcgcgguguguguggc
ggaagacgaccgcugugugugcaagcugugcggauggaggaggauuccga
cauuuugaggcugugcauu

[<<<<<<<<<<.......[..<<<<<<<<<..<......<<<<<<.....
...>>>.....<<<<<......>>>>>...>>>.....<<<<<<...>>>
>>>>....<<<<<<.....<......>......>>>>>.>.........<
<....>>...<<<<......<<.....>>.....>>>>...<<<......
....<<<<...>>>>...........<<<.<<.<<<.<.....<<<....
..>>>..((......<<<.....))>>>...>.>>>.>>.>>>.......
......<<<<<<<........>>>>>>>.....<<<<<..<.<<<>>>>>
..>>>........>>>>>>>.>>...............<<....>>....
]............................>>>>>>>>>>....<<<<...
....>>>>.<<<<........>>>><<<<<<....<<<<.<<<<.<<<..
...<<....>>....>>>.>>>>.>>>>..<<.<...<<<<<<.....<<
<<<<<<<..<<<....<<<<<<<....>>>>>>>.....>>>...<<<..
<<.<<........>>>>.>>>....>>>>>>>>>...<<.......>>..
....>>>>>>......>.>>..<<<<<<....<<<<<....>>>>><<<<
<<<<<....<<<<<<<<<...<<<...<<<<<<<.>>>>>>>..>.>>..
.>>>>>>>..>>...>>>>>>>.>>..<<<<<<<<<<.....>>>>>>>>
>>...<<<<......>>>>...<<<<<<...<<<.(....<<<<<.....
.<<<<...<<<<<<<............>>>>>>>..>>>>..........
.).......>>>>>.>>>...>>>>>>..<<<....>>>.....>>>>>>
<.<<<<<<<<<<......<.<<<<<<....>>>>>>.>...>>>>>>>>>
>..>.....>>>>>><<.....<<<<.<<<....>>><<<.<<<<<<.<<
...<<<<<<.>>>>>>....((<<<<<<......>>....<<<<......
..>>>>.))...<<<<<...<<<....>>>.........<<<<<<<....
<<<<<<<<<<<...((((...>>>>>>><.<<<<<<...<<.....>>..
....>>>>>>>.>>>>...>>>>>>>.............>>>>>...>>>
>..>>....<<......>>>>>>>><<<<.....>>>>...>>>......
...<<<<<<<<.<<.........<.....<<<.<....<<..>>.....>
.>>><<.<<<<<<..>>>>>>.>>..........<<<<<<<<<..<<<..
.......>>>..<<<<<<<........>>>>>>>...<<..<<<..<<<<
<<<<...<<<..<<<<<...>>>>>.>>>>>>>>>..>>>>><<<<<<..
.....>>>>>>........<...........<<<<<.......>>>>>..
...>.....>>....>>.>>>>>>>..>..>>.>>>>>>>>...>>>>..
..>>....<<<<<........>>>>>....].<<<..<<<.<<......<
<<<.<<<<<<..<<<<<.<<<<.<<..<<<<<<<<<<..>>>>>>>>>>.
.....>>.>>>>..>>>>>...<<<.....>>>..<.<<<<.........
..>>>>.>..<<...<<<<<<<...<<<.<<.......>>.>>>.....<
<...(((>>....>>>>>>>...<<<.<<<<<......>>>>>.>>>..<
<<....>>>.>>))).......<<<<<<<<<..>>>.>..>>>>>>>>>.
.........>>>>..>>>>>>.......<<.<.<.<<<<<<...<<....
...>>...>>>>>>...>>..>>.......<<<<<.<<<<<<..<<<<<<
<<........>>>>>..>>>.<<<.........>>>.....>>>>>..>.
..>>>.>>....<<<<<<<....>>>.>>>>....>>..>>>.>>>....
.<<<<<<<<<..<<<<..<<...............>>..>>>>.<<<<..
..<<<...<<<<<<.<<.)))).>>>>>>>>...>>>..>>>>...<<.<
...<<<<...........>>>>....>>>...>>>>>>>>>......<<<
<<.......>>>>>....<<<<<<<<<.....<<<<..<<<....>>>..
>>>>....>>>>.>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7qep Adaptation to genome decay in the structure of the smallest eukaryotic ribosome
• Resolution: 2.7 Å
• Binding residue (original residue number in PDB): R10 K35 A42 K50 A53 R54 T57 R60 K81 K86 R89 R93 R101 K103 T104 R105 R106 Q107 R108 A109 R110 M111 S112
• Binding residue (residue number reindexed from 1): R8 K33 A40 K48 A51 R52 T55 R58 K79 K84 R87 R91 R99 K101 T102 R103 R104 Q105 R106 A107 R108 M109 S110

Gene Ontology

Molecular Function

• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7qep, PDBe:7qep, PDBj:7qep
• PDBsum: 7qep
• UniProt: Q8ST62|RL351_ENCCU Large ribosomal subunit protein uL29A (Gene Name=RPL35A)