Structure of PDB 7ls1 Chain O2 Binding Site BS01

Receptor Information

>7ls1 Chain O2 (length=101) Species: 10090 (Mus musculus)

QVLKFTLDCTHPVEDGIMDAANFEQFLQERIKVNGKAGNLGGGVVTIERS
KSKITVTSEVPFSKRYLKYLTKKYLKKNNLRDWLRVVANSKESYELRYFQ
I

Ligand information

>7ls1 Chain A2 (length=3707)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggaaaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccgcgcgucgcggcgugggaaau
guggcguacggaagacccacuccccggcgccgcucguggggggcccaagu
ccuucugaucgaggcccagcccguggacggugugaggccgguagcggccc
ccggcgcgccgggcucgggucuucccggagucggguugcuugggaaugca
gcccaaagcgggugguaaacuccaucuaaggcuaaauaccggcacgagac
cgauagucaacaaguaccguaagggaaaguugaaaagaacuuugaagaga
gaguucaagagggcgugaaaccguuaagagguaaacgggugggguccgcg
caguccgcccggaggauucaacccggcggcgcguccggccgugccggugg
uccggcggaucuuucccgcgcgggggaccgcccccggccggcgaccggcc
gccgccgggcgcacuuccaccguggcggugcgccgcgaccggcuccggga
cggccgggaaggcccgguggggaagguggcucggggggggcucaccccga
guguuacagcccuccggccgcgcuuuucgccgaaucccggggccgaggaa
gcccgucgccgcgcucucccucucccgggugggggccgggccgccccucc
cacggcgcgaccgcucggggcggacuguccccagugcgccccgggcgucg
ucgcgccgucgggucccgggggcgucggucacgcgugacgaagccgagcg
cacggggucggcggcgaugucggcuacccacccgacccgucuugaaacac
ggaccaaggagucuaacgcgugcgcgagucaggggcucguccgaaagccg
ccguggcgcaaugaaggugaagggccccgcccgggggcccgaggugggau
cccgaggccucuccaguccgccgagggcgcaccaccggcccgucucgccc
gccgcgccggggagguggagcacgagcguacgcguuaggacccgaaagau
ggugaacuaugcuugggcagggcgaagccagaggaaacucugguggaggu
ccguagcgguccugacgugcaaaucggucguccgaccuggguauaggggc
gaaagacuaaucgaaccaucuaguagcugguucccuccgaaguuucccuc
aggauagcuggcgcucucgcuacgcaguuuuauccgguaaagcgaaugau
uagaggucuuggggccgaaacgaucucaaccuauucucaaacuuuaaaug
gguaagaagcccggcucgcuggcguggagccgggcguggaaugcgagugc
cuagugggccacuuuugguaagcagaacuggcgcugcgggaugaaccgaa
cgccggguuaaggcgcccgaugccgacgcucaucagaccccagaaaaggu
guugguugauauagacagcaggacgguggccauggaagucggaauccgcu
aaggaguguguaacaacucaccugccgaaucaacuagcccugaaaaugga
uggcgcuggagcgucgggcccauacccggccgucgccggcagucggaacg
gaacgggacgggagcggcgucgggccccgcggagccuacgccgcgacgag
uaggagggccgcugcggugagccuugaagccuagggcgcgggcccgggug
gagccgccgcaggugcagaucuuggugguaguagcaaauauucaaacgag
aacuuugaaggccgaaguggagaaggguuccaugugaacagcaguugaac
augggucagucgguccugagagaugggcgagugccguuccgaagggacgg
gcgauggccuccguugcccucggccgaucgaaagggagucggguucagau
ccccgaauccggaguggcggagaugggcgccgcgaggcguccagugcggu
aacgcgaccgaucccggagaagccggcgggagccucggggagaguucucu
uuucuuugugaagggcagggcgcccuggaauggguucgccccgagagagg
ggcccgugccuuggaaagcgucgcgguuccggcggcguccggugagcucu
cgcuggcccuugaaaauccgggggagaggguguaaaucucgcgccgggcc
guacccauauccgcagcaggucuccaaggugaacagccucuggcauguug
gaacaauguagguaagggaagucggcaagccggauccguaacuucgggau
aaggauuggcucuaagggcugggucggucgcggccggcgccuagcagccg
acuuagaacuggugcggaccaggggaauccgacuguuuaauuaaaacaaa
gcaucgcgaaggcccgcggcggguguugacgcgaugugauuucugcccag
ugcucugaaugucaaagugaagaaauucaaugaagcgcggguaaacggcg
ggaguaacuaugacucucuuaagguagccaaaugccucgucaucuaauua
gugacgcgcaugaauggaugaacgagauucccacugucccuaccuacuau
ccagcgaaaccacagccaagggaacgggcuuggcggaaucagcggggaaa
gaagacccuguugagcuugacucuagucuggcacggugaagagacaugag
agguguagaauaagugggaggcccggccgccggugaaauaccacuacucu
caucguuuuuucacugacccggugaggcgggggggcgagccccgaggggc
ucucgcuucuggcgccaagcguccgucccgcgcgugcgggcgggcgcgac
ccgcuccggggacagugccagguggggaguuugacuggggcgguacaccu
gucaaacgguaacgcagguguccuaaggcgagcucagggaggacagaaac
cucccguggagcagaagggcaaaagcucgcuugaucuugauuuucaguac
gaauacagaccgugaaagcggggccucacgauccuucugaccuuuugggu
uuuaagcaggaggugucagaaaaguuaccacagggauaacuggcuugugg
cggccaagcguucauagcgacgucgcuuuuugauccuucgaugucggcuc
uuccuaucauugugaagcagaauucaccaagcguuggauuguucacccac
uaauagggaacgugagcuggguuuagaccgucgugagacagguuaguuuu
acccuacugaugauguguuguugccaugguaauccugcucaguacgagag
gaaccgcagguucagacauuugguguaugugcuuggcugaggagccaaug
gggcgaagcuaccaucugugggauuaugacugaacgccucuaagucagaa
ucccgcccaggcggaacgauacggcagcgccgaggagccucgguuggccc
cggauagccggguccccguccugccccgcggccgggcgucgggaccgggg
uccggugcggagagccguucgucuugggaaacggggugcggccggaaagg
gggccgcccucucgcccgucacguugacgcacguucguguggaaccuggc
gcuaaaccauucguagacgaccugcuucugggucgggguuucguacguag
cagagcagcucccucgcugcgaucuauugaaagucagcccucgacacaag
gguuugu

.........................<<<<<<<....<......>.....>
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..>>>.>..<<<<<<<<<....<<<<<<<<.<<<....<<<<<<<<....
<<<<......>>>>.....((......<<<.....))>>>...>.>>>>>
>>>>>>>>>>>>>...>>>>>>>>>.......<<<<<<<........>>>
>>>>.....(((((..<<<<<<)))))..]]]..................
................<<....>>...............<<<......>>
>..................................<<<<<<<<..<<<.<
<...<<<<<<<<..<......<<<<<<<<<....<<<.<<<.<<<<..<<
....<<<<.<<<..<<<<<>>>>>>>>>>>>>>.>>>>>>>....>>>..
>>>>>>>>>........>.>>>.>>>>>...<<<<<<<.<<<<<<<<<<.
<<.....<<.<<<<.<<.<.<<......<<<<<<<<.<<..>>.>>>>>>
>>........>>>>>>>>>.......>>..>>...>>>>>>>>>>.....
..........<<<<<<.<<<<<.<<..>>.>>>>>...<<<..<<<<.<<
<<<<<<<<<<<<<<<<<<<<<<<...........>.>>>>>>>>>>>..>
>>>>>>>>>.>>>....>>>>..>>>.....<<.....>>.....>>>>>
>......>>.>>>>>...>>>>>...>>>>>>>>..<<<<........>>
>><<<<<<....<<<<<<<<<<<<<..<<<...<<<<........>>>>.
....>>>...<<....>>...<<<<<<<.....>>>>>>>..<<<<<...
<<<..<<<...(((.[[..>>>..>>>....>>>>>.<<<<<<<<<.<<<
<......>>>>>>>>>>>.>>....>>>>>>>>>>>>>..<<.<..<<<<
<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>>>.....>
>>...<<<..<..<<........>>.>.>>>....>>>>>>>>>...<<.
......>>......>>>>>>...>..>.>>..<<<<<<....<.<<<...
.>>>.><<<<<<<<<<.......<<<.<<<<<<<<<.<...<....<<<.
...<<<<.....<<..........>>..>>>>....>>>..>.....>>>
>>>>>...<<<<<<<<<.<......>>>>>>>>>>....>>>>>>>>>>>
>.>>>..<<<<.<<<<<.....>>>>>.>>>>....<<<......>>>..
.<<<<<<<...<<<(((.......<<<<<.....<<..........<<<.
<<<<<<<<<<.......<<<<<.<<....<<.........>>....>>..
.......<<......>>...>>>>>...>>>>>>>>>>>>>.........
.))).>>...>>>>>..>>>...>>>>>>><<<<<<.<<<..........
..]].)))...<<<...>.>>..................>>>>>>>>>..
..>>>>>>.<.<<<<<<<..<<......<<<<.<<<<....>>>>>>>>.
..>>.>>>>>>>..>.....>>>>>><<<....<<.....<<<<<<....
...>>>>>>.<<<.....<<<<<.<<...<<<<<<...<<.....>>.>>
>>>>....((<<<<<<...<<....<<<....>>>..........<<.<<
<<<.<<<...>>>.>>>>>>>......>>....>>...<<<<<.......
.>>>>>))<<<<....<<<<<...<<<<<<<....>>>>>>>...<<<..
..>>>...<..<<<<<<<...<<<<<<<<<<<<..<<<<<<<<<..{{{.
.>>>>>>....<<<<<.<<<<.<<<......<<<.....>>>......>>
>>>>>..>>>>>.....<<<<<<<.......>>>>>>>>>>....>>>>>
>>>>>>>.........>>>>>>>..>..........>>.>>>.>>>>>>>
>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>....<<
<<.....<<<<<<<.<<.........<.....<<<<<....<....>...
..>>>>><<.<<<<<<<<<<<...<..........>........>>>>>.
.>>>>>>.>>............<<<<<<<<<..<<<.........>>>..
<<<<<<<....<<<<.....>>>>......>>>>>>>....<<.<<<..<
<<<<<....<<<.....<<<<....>>>>.....>>>.>>>>..>>>>>.
<<<<<.......>>>>>.........<...........<<<<<.......
>>>>>.....>.....>>....>>.>>>>>>>..>..>>.>>>>>>>..>
>>>>>...>>>....<<<<<........>>>>>....[[[[[..{{{.{{
......[[[[.{{[[[[..<<<<<.<<<<<<<<<.<<<<<<<<<<<<..<
<<...........<..............................>.>>>.
.>>.>>>>>>>>>>...<<<<<.....<.<<<....<<<<.<<...>>>>
>>................<<<<<<<.<<<<<....>>>>>>>>>>>>..>
>>>..>>>>>....>>>>>>>>>..>>>>>...<<<.....>>>..<<<<
<<...........>>>>>>..<<....<<<<<<...<<<<<<.......>
>>>>>.....<<...(((>>....>>>>>>....<<<..<<<<.......
>>>>..>>>.<<<....>>>.>>))).......((((((..(((..>>>>
>>.)))))))))..........]]]]..}}]]]].......<<.<.<.<<
<<<<...<<.......>>...>>>>>>...>>..>>.......<<<<<.<
<<<<<..<.<.<<<<<......>>>>>...>.>.<<<.........>>>.
....>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>....}}
..}}}.]]]]].....<<<<<<..<.<<<..<.<<<<<............
.....>>>>>.><<<<....<<<<....<<.<<<<<<<}}}.>>>>>..>
>.>>...>>>>...>>>><<<<....<<<<<...........>>>>>...
.>>>>.>>>...>.>>>>>>.....<<<<<<.<<<..<<.<....<<<..
......<<<<<<.<<<<<..<<<.....<<<>>>.....>>.>..>>>>>
>>>>>>.......>>>...<<<..<<.<....<<<<.<<<<<<.......
.>>>>>>>>>>....>>>..>>>...............>.>>..>>>>>>
>>><<<<<.<.............>....<<.<<<<<<<.<<.....<<<<
....<<<<<......>>>>>...>>>>....>>....>>>>.>>>.>>..
>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7ls1 Functionally distinct roles for eEF2K in the control of ribosome availability and p-body abundance.
• Resolution: 3.3 Å
• Binding residue (original residue number in PDB): R46 K48 N50 S79 K80 R81 Y82 K84 Y85 K89 K92 V103 A104 K107 Y110
• Binding residue (residue number reindexed from 1): R30 K32 N34 S63 K64 R65 Y66 K68 Y69 K73 K76 V87 A88 K91 Y94

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0008201 heparin binding
• GO:0042802 identical protein binding

Biological Process

• GO:0006412 translation
• GO:0046632 alpha-beta T cell differentiation
• GO:0140236 translation at presynapse
• GO:0140242 translation at postsynapse

Cellular Component

• GO:0005634 nucleus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:0045202 synapse
• GO:0098793 presynapse
• GO:0098794 postsynapse
• GO:0098978 glutamatergic synapse
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7ls1, PDBe:7ls1, PDBj:7ls1
• PDBsum: 7ls1
• PubMed: 34815424
• UniProt: P67984|RL22_MOUSE Large ribosomal subunit protein eL22 (Gene Name=Rpl22)