Structure of PDB 8cah Chain O Binding Site BS01

Receptor Information

>8cah Chain O (length=312) Species: 580240 (Saccharomyces cerevisiae W303)

EVLVLRGTLEGHNGWVTSLATSAGQPNLLLSASRDKTLISWKLTGDDQKF
GVPVRSFKGHSHIVQDCTLTADGAYALSASWDKTLRLWDVATGETYQRFV
GHKSDVMSVDIDKKASMIISGSRDKTIKVWTIKGQCLATLLGHNDWVSQV
RVVPNEKADDDSVTIISAGNDKMVKAWNLNQFQIEADFIGHNSNINTLTA
SPDGTLIASAGKDGEIMLWNLAAKKAMYTLSAQDEVFSLAFSPNRYWLAA
ATATGIKVFSLDPQYLVDDLRPEFAGYSKAAEPHAVSLAWSADGQTLFAG
YTDNVIRVWQVM

Ligand information

>8cah Chain 2 (length=1745)

uaucugguugauccugccaguagucauaugcuugucucaaagauuaagcc
augcaugucuaaguauaagcaauuuauacagugaaacugcgaauggcuca
uuaaaucaguuaucguuuauuugauaguuacaugguauaacugugguaau
ucuagagcuaauacaugcuuaaaaucucgacccuuuggaagagauguauu
uauuagauaaaaaaucaaugucuucggacucuuugaugauucauaauaac
uuuucgaaucgcauggccuugugcuggcgaugguucauucaaauuucugc
ccuaucaacuuucgaugguaggauaguggccuaccaugguuucaacgggu
aacggggaauaaggguucgauuccggagagggagccugagaaacggcuac
cacauccaaggaaggcagcaggcgcgcaaauuacccaauccuaauucagg
gagguagugacaauaaauaacgauacagggcccauucgggucuuguaauu
ggaaugaguacaauguaaauaccuuaacgaggaacaauuggagggcaagu
cuggugccagcagccgcgguaauuccagcuccaauagcguauauuaaagu
uguugcaguuaaaaagcucguaguugaacuuugggcccgguuggccgguc
ggauuuccaacggggccuuuccuucuggcuaaccuugagcuuggcgaacc
aggacuuuuacuuugaaaaaauuagaguguucaaagcaggcguauugcuc
gaauauauuagcauggaauaauagaauaggacguuugguucuauuuuguu
gguuucuaggaccaucguaaugauuaauagggacggucgggggcaucagu
auucaauugucagaggugaaauucuuggauuuauugaagacuaacuacug
cgaaagcauuugccaaggacguuuucauuaaucaagaacgaaaguuaggg
gaucgaagaugaucagauaccgucguagucuuaaccauaaacuaugccga
cuagggaucgggugguguuuuuuuaaugacccacucggcaccuuacgaga
aaucaaagucuuuggguucuggggggaguauggucgcaaggcugaaacuu
aaaggaauugacggaagggcaccaccaggaguggagccugcggcuuaauu
ugacucaacacggggaaacucaccagguccagacacaauaaggauugaca
gauugagagcucuuucuugauuuugugggugguggugcauggccguucuu
aguugguggagugauuugucugcuuaauugcgauaacgaacgagaccuua
accuacuaaauaguggugcuagcauuugcugguuauccacuucuuagagg
gacuaucgguuucaagccgauggaaguuugaggcaauaacaggucuguga
ugcccuuagacguucugggccgcacgcgcgcuacacugacggagccagcg
agucuaaccuuggccgagaggucuugguaaucuugugaaacuccgucgug
cuggggauagagcauuguaauuauugcucuucaacgaggaauuccuagua
agcgcaagucaucagcuugcguugauuacgucccugcccuuuguacacac
cgcccgucgcuaguaccgauugaauggcuuagugaggccucaggaucugc
uuagaucagagcggagaauuuggacaaacuuggucauuuagaggaacuaa
aagucguaacaagguuuccguaggugaaccugcggaaggaucauu

...<<<<<.{.((((>>>>><<<<.<<<<<<...<.<...<......<<<
.<<<..<<....<<....<<..........>>...>>.>>......<<..
......<<<..<<..<<....<<<..........<.....<<.<<<....
.>>>.>>......>.........<<<<<..<<....>>..>>>>><...<
<<<<<......(((.{((.........................>>>>>>.
....>...<<<<..<<<.....>>>.>>>>....>>>...>>>>..>>>.
<<<....<<<....<<<<<<<<.......>>>>>>>>>>>......>>>.
..<<<.<<<<....>>>>....>>>.>>.<<.<<<..........>>>.>
>.<.<<....>>.>...>>>>>>.........<<<....<<<.....>>>
..>>>..>....>.>.....<<<<<<<...<<......>>...>>>>.>>
>......<<..<...........>..>>.........<<<<<((......
<<<<.....<<..))>>.......>>>>.>>>>>..>>>>>>>>>>....
.....<<<[[.....<.<<...<<<.<<....<<<<<<.<<<<<......
....>..>>>>.>>>>>>.....<<<<<....................>>
>>>....<<<<<.<<.......<<...<.......>..<<<.....>>>.
...>>......>>.>>..>>>.........<<<..<<<<<<<<.....))
.}))).>>>>>>>>.>>>.....>>....<<<<<<.<<...<<<<..<<.
.<<<<<<.<...<<<......>>>......>.>>>>>>..>>.......<
<....>>...>>>>...>>>>>.>>>...>>>...>>.>....<<<<<<<
...<...<<<<.<.....>.>>>>...>>>>>>>>..........<<<.<
<.<<<..<<<<<<<<.<<<........>>>>>>>>>>>..>>>...<<..
]]>>...>>.....>>>.>>>.<<<......<<<<....>>>>....>>>
..)))).}<<<<<.<<<<<<<..<<.<<<<<<..<<<.<<<<<<......
<<........>>..........<<<<<.<....<<<<<........<<.<
<<........>>>.>>......>>>>>...<<.<<<..<<.<<<<<<...
.<<<.<<<<<....>>>...<<<......>>>...>>.>>>....<<<<<
.<<..<<<<..<<<<<.<<<<<<....>>>>>>...>>>>>..>>>>.>>
....<<<<<<.....>>>>>>.......>>>>>....>>>.>>>.....>
>>>>>>.....>.>>>>>...>>.>>>>.>>>.....<<<<<<<......
<<.....<<..<<<<....>>>>..>>....>>.......>>>>>>>...
...<....<<<<<<..........>>>>>>....>.....>>>>>>....
<<<<<<<<.......>>>>>>>>......>>...>>>>>>>>>>.>>...
.<..<<.<..<<<<.<<....<<<<<<<<.<<<..<.<<..<...<<<<<
<<.<..>.>>>>>>>...>..>>.>..>>>.>>>>>>>>...>>.>>>>.
..>.>>...>.....<<<<<<<<<<..>>>>>>>>>>........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8cah Molecular basis for recognition and deubiquitination of 40S ribosomes by Otu2.
• Resolution: 3.0 Å
• Binding residue (original residue number in PDB): G63 H66 R102 K283 A284
• Binding residue (residue number reindexed from 1): G59 H62 R98 K279 A280

Gene Ontology

Molecular Function

• GO:0001965 G-protein alpha-subunit binding
• GO:0005080 protein kinase C binding
• GO:0005092 GDP-dissociation inhibitor activity
• GO:0043022 ribosome binding
• GO:0045182 translation regulator activity

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006521 regulation of cellular amino acid metabolic process
• GO:0007186 G protein-coupled receptor signaling pathway
• GO:0010629 negative regulation of gene expression
• GO:0017148 negative regulation of translation
• GO:0061157 mRNA destabilization
• GO:0070651 nonfunctional rRNA decay
• GO:0072344 rescue of stalled ribosome
• GO:1902660 negative regulation of glucose mediated signaling pathway
• GO:1990116 ribosome-associated ubiquitin-dependent protein catabolic process
• GO:1990145 maintenance of translational fidelity
• GO:2001125 negative regulation of translational frameshifting

Cellular Component

• GO:0005634 nucleus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8cah, PDBe:8cah, PDBj:8cah
• PDBsum: 8cah
• PubMed: 37169754
• UniProt: P38011|GBLP_YEAST Small ribosomal subunit protein RACK1 (Gene Name=ASC1)