Structure of PDB 8azw Chain O Binding Site BS01

Receptor Information

>8azw Chain O (length=49) Species: 4097 (Nicotiana tabacum)

AKSKNHTAHNQSYKAHRNGIKKPRKHRHSSTKGMDPKFLRNQRYARKHN

Ligand information

>8azw Chain A (length=3196)

gcgaccccaggucaggcgggacuacccgcugaguuuaagcauaucaauaa
gcggaggaaaagaaacuuacaaggauuccccuaguaacggcgagcgaacc
gggaacagcccagccuuagaaucgggcggcuccgccguucgaauuguagu
cuggagaagcguccucagcggcggaccgggcccaaguccccuggaagggg
gcgccagagagggugagagccccguugugcccggacccugucgcaccacg
aggcgcugucuacgagucggguuguuugggaaugcagcccaaaucgggcg
gugaauuccguccaaggcuaaauacgggcgagagaccgauagcgaacaag
uaccgcgagggaaagaugaaaaggacuuugaaaagagagucaaagagugc
uugaaauugucgggagggaagcggaugggggccggcgaugcgccccgguc
ggauguggaacggugacgagccgguccgccgaucgacucggggcguggac
cagcguggauugggggggcggccaaagcccgggcuuucgauacgcccgug
gaacgccgucuccuugauugugguaggcagcgcgcgccuccggcgugcuu
cggcaucugcgcgcuccggacgcuggccugugggcuccccauucgacccg
ucuugaaacacggaccaaggagucugacaugugugcgagucaacgggcga
guaaacccguaaggcguaaggaagcugauuggugggaucccccugagggg
ugcaccgccgaccgaccuugaucuucugugaaggguucgagugugagcau
accugucgggacccgaaagauggugaacuaugccugagcggggcgaagcc
agaggaaacucugguggaggcccgcagcgauacugacgugcaaaucguuc
gucugacuuggguauaggggcgaaagacuaaucgaaccgucuaguagcug
guucccuccgaaguuucccucaggauagcuggagcucgcgugcgaguucu
aucggguaaagccaaugauuagaggcaucgggggcgcaacgcccucgacc
uauucucaaacuuuaaauagguaggacggugcggcugcuuuguugagccg
caccacggaaucaagagcuccaagugggccauuuuugguaagcagaacug
gcgaugcgggaugaaccggaagccggguuacggugccaaacugcgcgcua
accuagaucccacaaaggguguuggucgauuaagacagcgccgaaucaac
uagccccgaaaauggauggcgcuuaagcgcgcgaccuacacccggccguc
ggggcaagugccaggccccgaugaguaggagggcgcggcggucgcugcaa
aaccuugggcgcgagccugggcggagcggccgucggugcagaucuuggug
guaguagcaaauauucaaaugagaacuuugaaggccgaagaggggaaagg
uuccaugugaacggcacuugcacauggguuagucgauccuaagggucggg
ggaaccccgacagauagcgcguuucgcgcguacuccgaaagggaaucggg
uuaaaauuccugaaccgggacguggcgguugacggcaacguuaggaaguc
cggagacgucggcgggggccucgggaagaguuaucuuuucuguuuaacag
ccugcccacccuggaaacgacucagucggagguaggguccagcggcugga
agagcaccgcacgucgcgugguguccggugcgcccccggcggcccuugaa
aauccggaggaccgagugccguccacgcccggucguacucauaaccgcau
caggucuccaaggugaacagccucuggucgauggaacaauguaggcaagg
gaagucggcaaaauggauccguaacuucgggaaaaggauuggcucugagg
gcugggaacagccaacucagaacugguacggacaaggggaauccgacugu
uuaauuaaaacaaagcauugcgauggucccugcggauguuuacgcaaugu
gauuucugcccagugcucugaaugucaaagugaagaaauucaaccaagcg
cggguaaacggcgggaguaacuaugacucucuuaagguagccaaaugccu
cgucaucuaauuagugacgcgcaugaauggauuaacgagauucccacugu
cccugucuacuauccagcgaaaccacagccaagggaacgggcuuggcaga
aucagcggggaaagaagacccuguugagcuugacucuaguccgacuuugu
gaaaugacuugagaggguaguauaagugggagccggcgaaagugaaauac
cacuacuuuuaacguuauuuuacuuauuccgugaaucggaagcggggcac
ugccccucuuuuuggacccaaggcuugcuugcaggccgauccgggcggaa
gacauugucagguggggaguuuggcuggggcggcacaucuguuaaaagau
aacgcagguguccuaagaugagcucaacgagaacagaaaucucgugugga
acagaaggguaaaagcucguuugauucugauuuccaguacgaauacgaac
cgugaaagcguggccuaacgauccuuuagaccuucggaauucgaagcuag
aggugucagaaaaguuaccacagggauaacuggcuuguggcagccaagcg
uucauagcgacguugcuuuuugauccuucgaugucggcucuuccuaucau
ugugaagcagaauucaccaaguguuggauuguucacccaccaauagggaa
cgugagcuggguuuagaccgucgugagacagguuaguuuuacccuacuga
ugacagugucgcaauaguaauucaaccuaguacgagaggaaccguugauu
cgcacaauuggucaucgcgcuugguugaaaagccaguggcgcgaagcuac
cgugcgcuggauuaugacugaacgccucuaagucagaauccgggcuagaa
gcgacgcaugcgcccgccguccgcuugccgacccgcaguaggggccuuug
gcccccaagggcacgugucguuggcuaagucaucguggcggaagagccgc
gguggccgccuugaaguacaauuuccaucgagcggcggguagaauccuuu
gcagacgacuuaaauacgcgacgggguauuguaaguggcagaguggccuu
gcugccacgauccacugagauucagcccuuugucgcuccgauucgu

........................<<<<<<.....<......>......>
>>>>.>.........<<....>>..<<<<<.......<.....>......
>>>>>..[[[....<......<<<<<<<<<...>>>>>>>>>......>.
....<<<<<<<<.<<<.<<<<<<<.<<<<<<.<<...<<<<......>>>
>.((......<<<.....))>>>..>>.>>>>>>...>>>>>>>.....>
>>>>>>>.>>>.......<<<<<<<........>>>>>>>.....(((((
..<<<<<<)))))..]]]................................
..<<....>>...............<<<<....>>>>.............
.....................<<<<<<<<<<<<......<<<<<<<<<<<
<...<<<<<.<<<<.....>>>>.>>>>>....>>>>.>>>>>>>>.<.<
<<<<<..<<<<<<<<<<<<<<<.....<<<<<<<.........>>>>>.>
>...>>>>>>>>>>>>>>>.........<<<<<<<<<<...>>>..<<..
..>>....>>>>>>>.....>>>>>>.>....>>>.>>>>>>>>>..<<<
<........>>>><<<<<<.....<<<<<.<<<<<<..<<<...<<<...
.....>>>....>>>...<<....>>....<<<<<...<<<<....>>>>
....>>>>>.<<<<<<<.....<....>.....>>.>>>.>>....>>>>
>>.>>>>>...<<.<..<<<<<<<.....<<<<<<<<<..<<<....<<<
<<<<....>>>>>>>.....>>>...<<<<.<<.<<........>>>>>>
>>....>>>>>>>>>...<<.......>>......>>>>>>...>..>.>
>..<<<<<<....<.<<<....>>>.><<<<<<<<<<......<<.<<<<
<<<.....<<<....<<<....<<<....<<<<<<.....>>>>>>..>>
>....>>>..>.>>.....>>>>>...<<<<<<<<<.<......>>>>>>
>>>>....>>>>..>>>>>>>>.>>..<<<<.<<<<<.....>>>>>.>>
>>....<<<......>>>...<<<<<<<...<<<(((.....<<<<<<..
.....<..........<<<.<<<<<<.<<<.......<..>...>>>.>>
>>>>>>>..........))).>....>>>>>>.>>>...>>>>>>><<<<
<<<<<.........>>>>>>>>>....>>>>>><.<<<<<<<<<<.....
..<<<.<<<<....>>>>>>>....>>>>>>>>>>..>.....>>>>>><
<.....<<.....<<<<<<.......>>>>>><<<<.....<<<<<.<<.
..<<<<<<...<<.....>>.>>>>>>....((<<<<<<...<<....<<
<....>>>........<<<<<...>>>>>.....>>....>>...<<<<<
........>>>>>))<<<<.<<<<<<<<...<<<....>>>...<...<<
<<<<...<<<<.<<<<<<<.<<<<<.<<<<.......>>>>.><...<<<
<<.....<<<<<<....<<<<...>>>>....>>>>>>.....>>>>>..
..><<<<<<<.......>>>>>>>>>>>...>>>>>>>.>>>>.......
..>>>>>>...>......>>>>.>>>>>>>>>>>>..>>.....<<....
..>>>>>>>.<<<<.....>>>>..>>>>..<<<<.....<<<<<<<.<<
.........<.....<<<<<....<<..>>.....>>>>><<.<<<<<<<
<<<<....>>>>>..>>>>>>.>>............<<<<<<<<<..<<<
.........>>>..<<<<<<<....<<<<....>>>>......>>>>>>>
...<<..<<<..<<<<<<.<..<<<.....<<<<....>>>>.....>>>
>>>>>..>>>>><<<<<<.......>>>>>>........<..........
.<<<<<.......>>>>>.....>.....>>....>>.>>>>>>>..>..
>>.>>>>>>>..>>>>>>....>>....<<<<<........>>>>>....
[[[[[..{{{.{{......[[[[.{{[[[[..<<<<<.<<<.<<<...<<
<<<<<<<<.<<<<<<.........<<<<.<.<<<>>>............>
>>>>.>>>>>>..>>>>>>>>>>...<<<<<.....<<<<...<<<<<..
.>>>>>...............<<<<<<....>>>>>>..>>>>..>>>>>
......>>>.>>>..>>>>>...<<<.....>>>..<<<<<<........
...>>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....
<<...(((>>....>>>>>>>...<<<.<<<<<.......>>>>.>>>>.
<<<....>>>.>>))).......((((((..((((.>>>>>>))))))))
))..........]]]]..}}]]]].......<<.<.<.<<<<<<...<<.
......>>...>>>>>>...>>.>.>.......<<<<<.<<<<<<..<<<
.<<<<<......>>>>>...>>>.<<<.........>>>.....>>>>>.
.>...>>>.>>....<<<<<<<....>>>.>>>>....}}..}}}.]]]]
]....<<<<<<<..<<<<.<.<<<<<.................>>>>>.>
<<<<<...<<<<....<<<<<<<<<......>>>>..>>>>>...>>>>.
.>>>>>.<<<<...<<<<<...........>>>>>...>>>>.>>>>...
>>>>>>>....<<<<<<<<<.<<.<......<<.......<<<<<<...>
>>>>>....>>...........<<<...<<<<<<<<<<<......>>>>>
>>>>>>.>>>................>.>>.>>>>>>>>>.<<<<<.<..
...........>.....<<<<<<<<<.<<<....<<<<.<..<<<<<...
...>>>>>..>>>>>...>>>....>>>>..>>>>>...>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8azw Structure of the actively translating plant 80S ribosome at 2.2 angstrom resolution.
• Resolution: 2.14 Å
• Binding residue (original residue number in PDB): A2 K3 K5 N6 H7 T8 A9 H10 N11 Q12 S13 K15 H17 R18 N19 R25 K26 H27 R28 H29 S31 T32 D36 P37 K38 R41 N42 Q43 R44 Y45 A46 R47 H49 N50
• Binding residue (residue number reindexed from 1): A1 K2 K4 N5 H6 T7 A8 H9 N10 Q11 S12 K14 H16 R17 N18 R24 K25 H26 R27 H28 S30 T31 D35 P36 K37 R40 N41 Q42 R43 Y44 A45 R46 H48 N49

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8azw, PDBe:8azw, PDBj:8azw
• PDBsum: 8azw
• PubMed: 37156858
• UniProt: A0A1S3YR12